Sensing and Bio-Sensing Research最新文献_第5页

Electrochemical molecularly imprinted polymer (MIP) sensor for progressive monitoring of antibody production ex vivo 电化学分子印迹聚合物（MIP）传感器用于体外抗体生产的渐进监测

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2026-03-03 DOI: 10.1016/j.sbsr.2026.100991

Kyung Eun You, Joshua Cheng, Jeong Ok Lim, James J. Yoo, Sang Jin Lee, Shay Soker

{"title":"Electrochemical molecularly imprinted polymer (MIP) sensor for progressive monitoring of antibody production ex vivo","authors":"Kyung Eun You, Joshua Cheng, Jeong Ok Lim, James J. Yoo, Sang Jin Lee, Shay Soker","doi":"10.1016/j.sbsr.2026.100991","DOIUrl":"10.1016/j.sbsr.2026.100991","url":null,"abstract":"<div><div>Dynamic monitoring of antibody production is essential for understanding immune responses. Conventional assays like ELISA offer only static measurements and lack temporal representation. To address this limitation, we developed an electrochemical biosensor using molecularly imprinted polymers (MIPs) for rapid, label-free detection of immunoglobulins (IgGs). Our platform enables progressive assessment of IgG production <em>ex vivo</em> through sequential electrochemical measurements. The MIP sensor was fabricated <em>via</em> electropolymerization of <em>o</em>-phenylenediamine and pyrrole in the presence of the Fc fragment of IgG as a template. Selective IgG recognition cavities were formed through combined chemical and electrochemical template removal. An <em>ex vivo</em> platform was established using hybridoma cells encapsulated in hydrogel beads to simulate continuous antibody production over seven days. The sensor detected mouse IgG in the range of 1.56–50 ng/mL with a strong linearity (R<sup>2</sup> = 0.956) and a limit of detection of 1.54 ng/mL, and it also demonstrated the capability to detect IgG in human serum samples. IgG production was progressively monitored in hybridoma culture medium, with temporal trends consistent with ELISA measurements. By Day 7, the ratio of IgG concentration measured by the MIP sensor to that determined by ELISA was approximately 0.95. This study demonstrates the feasibility of the MIP sensor for fast, label-free IgG detection, providing a practical and efficient platform alternative to traditional assays.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100991"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147419799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waveform-aware deep learning for quantitative agglutination on a low-cost lab-in-tubing microfluidic platform 基于低成本管内实验室微流控平台的定量凝集的波形感知深度学习

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2026-02-19 DOI: 10.1016/j.sbsr.2026.100983

Rohith Jayaraman Krishnamurthy, Justin Farrell, Abdul Basit Zia, Abbas S. Milani, Ian G. Foulds

{"title":"Waveform-aware deep learning for quantitative agglutination on a low-cost lab-in-tubing microfluidic platform","authors":"Rohith Jayaraman Krishnamurthy, Justin Farrell, Abdul Basit Zia, Abbas S. Milani, Ian G. Foulds","doi":"10.1016/j.sbsr.2026.100983","DOIUrl":"10.1016/j.sbsr.2026.100983","url":null,"abstract":"<div><h3>Objective</h3><div>To enable quantitative agglutination readout using an ultra-low-cost (parts cost < $40) lab-in-tubing optical platform.</div></div><div><h3>Methods</h3><div>Droplet waveforms were recorded with dual infrared line-break sensors from biotinylated BSA mixed with streptavidin-coated beads at nine levels (0, 0.0128–1000 μg mL-1; <em>N</em> = 223 droplets, <em>N</em> = 211 after QC). We apply waveform-aware preprocessing (i.e., preprocessing operations defined on the full time-series waveform that preserve concentration-dependent morphology rather than reducing the signal to a single scalar) (length normalization, Hampel spike suppression, offset-drift compensation, quality-control filtering, and blank (0 μg mL-1) reference subtraction) and train a one-dimensional convolutional neural network (1D-CNN) to regress log10(concentration). Results: On a stratified 80/20 validation split, the model achieved MAE = 30.49 μg mL-1 with sub-μg absolute errors at trace-to-mid concentrations and larger but scale-appropriate deviations near the apex (∼8 μg mL-1) and in the prozone. Leave-one-concentration-out (LOCO) experiments show that errors increase primarily at omitted calibration anchors, motivating a minimal three-anchor scheme (low, apex, high/prozone) for stable interpolation. Conclusion: Coupling inexpensive optics with waveform-level modeling supports quantitative agglutination estimation across nearly five orders of magnitude (0.0128–1000 μg mL-1). Multi-day, multi-device, and matrix-effect validation remain future work. LOCO is a special case of leave-group-out cross-validation, and we use it here to emulate missing calibration anchors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100983"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147421382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental and finite element analysis of coil position effects in inductive sensors using a design of experiments approach 采用实验设计的方法对电感式传感器线圈位置效应进行了实验和有限元分析

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2026-02-19 DOI: 10.1016/j.sbsr.2026.100985

Morteza Bagheri, Amir Musa Abazari

{"title":"Experimental and finite element analysis of coil position effects in inductive sensors using a design of experiments approach","authors":"Morteza Bagheri, Amir Musa Abazari","doi":"10.1016/j.sbsr.2026.100985","DOIUrl":"10.1016/j.sbsr.2026.100985","url":null,"abstract":"<div><div>Planar coils offer advantages such as a two-dimensional structure and compact design, making them suitable for various applications. They are widely used in position sensing, proximity detection, and non-contact measurement. Planar coils rely on mutual inductance as a key parameter. The mutual inductance changes with the relative displacement between two coils. The mutual inductance variation is the basis of inductive sensors. This study includes both experimental and finite element investigations of the effect of coil displacement on mutual inductance. In the experimental setup, a special fixture was designed to enable the movement of one coil with six degrees of freedom, allowing precise control of both axial and angular displacements. First, the accuracy of the finite element model was validated by comparing its results with those from a reference study, yielding an R<sup>2</sup> score between 0.9377 and 0.9721. The mutual inductance between two coils was evaluated under different conditions using both methods. The Taguchi method was employed to reduce the number while maintaining accuracy. The signal-to-noise ratio analysis showed that minimizing the vertical gap between the coils enhances magnetic coupling, while x-axis rotation has the least effect.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100985"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147421383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-inspired linseed derived carbon quantum dots as a fluorescent probe for the fluorimetric determination of imeglimin in biological fluids and dosage forms 生物启发亚麻籽衍生的碳量子点作为荧光探针，用于荧光测定生物液体和剂型中的伊米霉素

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2025-12-20 DOI: 10.1016/j.sbsr.2025.100946

Aya Barseem , Reem H. Obaydo , Samar H. Elagamy

{"title":"Bio-inspired linseed derived carbon quantum dots as a fluorescent probe for the fluorimetric determination of imeglimin in biological fluids and dosage forms","authors":"Aya Barseem , Reem H. Obaydo , Samar H. Elagamy","doi":"10.1016/j.sbsr.2025.100946","DOIUrl":"10.1016/j.sbsr.2025.100946","url":null,"abstract":"<div><div>This study demonstrates the first fluorimetric method for the determination of Imeglimin IMG, a novel antidiabetic drug. Since IMG lacks native fluorescence, a novel bio-inspired linseed derived carbon quantum dots LIN-CQDs were utilized as a fluorescent probe for its determination. The LIN-CQDs exhibited strong native emission at 470 nm upon excitation at 380 nm. Comprehensive characterization of the synthesized LIN-CQDs was performed using transmission electron microscopy TEM, energy-dispersive X-ray spectroscopy EDX, and X-ray photoelectron spectroscopy XPS, which confirmed the successful formation of nitrogen-doped CQDs. The fluorescence intensity of LIN-CQDs was quenched progressively with increasing concentrations of IMG. The mechanism of quenching was studied and it was found to follow a static quenching process. The quenching response showed excellent linearity over the concentration range of 0.15–3.0 μg/mL. All experimental conditions influencing the quenching process were carefully optimized. The method was validated in compliance with ICH guidelines, demonstrating high accuracy, precision, and specificity. The results of statistical comparison with a previously reported HPLC method confirmed no evident difference between the two approaches for the assay of the drug in its dosage form. Furthermore, the developed method was successfully applied for the assay of IMG in biological fluids including spiked human plasma and urine. The sustainability of the developed method was evaluated using the Analytical Eco-Scale, AGSA, and ComplexMoGAPI, metrics. Owing to its simplicity, sensitivity, and sustainability, the proposed fluorimetric assay provides a green alternative for routine determination of IMG in pharmaceutical analysis.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100946"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomolecule analysis using portable absorption spectrophotometer with novel spatial filter 新型空间滤波器便携式吸收分光光度计的生物分子分析

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2026-01-07 DOI: 10.1016/j.sbsr.2026.100955

Tomoki Tahara , Mami Akaike , Yutaro Arakaki , Kinichi Morita , Yoichi Saito , Yoshitaka Nakanishi , Yuta Nakashima

{"title":"Biomolecule analysis using portable absorption spectrophotometer with novel spatial filter","authors":"Tomoki Tahara , Mami Akaike , Yutaro Arakaki , Kinichi Morita , Yoichi Saito , Yoshitaka Nakanishi , Yuta Nakashima","doi":"10.1016/j.sbsr.2026.100955","DOIUrl":"10.1016/j.sbsr.2026.100955","url":null,"abstract":"<div><div>Biomolecules reflect the onset and progression of disease as well as overall health status, rendering their quantification essential. Although various techniques exist for biomolecule quantification, colorimetric analysis is widely used owing to its simplicity and rapid results. However, conventional absorption spectrophotometers used in such analyses suffer from measurement errors caused by stray light, such as reflected and scattered light, which compromise accuracy. This study introduces a portable absorption spectrophotometer with a novel spatial filter featuring a light-guiding structure designed to suppress stray light. This innovation enables rapid and highly sensitive quantification of biomolecules in the field. Using the proposed device, biomolecular samples including proteins and amino acids were analyzed. The results demonstrated a high linearity in the calibration curve (<em>R</em><sup>2</sup> > 0.99), confirming a reliable correlation between absorbance and concentration. Real protein samples, such as serum and immune cells, were quantified with accuracies comparable to those of commercial spectrophotometers, further validating the applicability of the device. These findings highlight the device's potential as an effective tool for biomolecule evaluation in various environments, offering portability, sensitivity, and reliability.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100955"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical determination of Pb(II) in paint factory effluent and hot spring water samples using chitosan-based Schiff base modified carbon paste electrode 壳聚糖基希夫碱改性碳糊电极电化学测定油漆厂废水和温泉水样品中的铅

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2025-12-08 DOI: 10.1016/j.sbsr.2025.100928

Kidus Ayalew Alemu , Genet Nurga Ansa , Tolera Marga Goshu , Mamo Gebrezgiabher , Siraye Esubalew Debebe , Tesfu Hailu Halefom

{"title":"Electrochemical determination of Pb(II) in paint factory effluent and hot spring water samples using chitosan-based Schiff base modified carbon paste electrode","authors":"Kidus Ayalew Alemu , Genet Nurga Ansa , Tolera Marga Goshu , Mamo Gebrezgiabher , Siraye Esubalew Debebe , Tesfu Hailu Halefom","doi":"10.1016/j.sbsr.2025.100928","DOIUrl":"10.1016/j.sbsr.2025.100928","url":null,"abstract":"<div><div>Pb(II) is a hazardous and non-biodegradable metal widely used in paint manufacturing and mining industries, posing health risks such as neurological disorders, kidney dysfunction, and increased blood pressure. Therefore, developing an easy, selective, and sensitive sensor for its detection is crucial. In this study, a chitosan-vanillin Schiff base (CVSB) was synthesized, and its successful formation was confirmed using spectroscopic and electrochemical techniques. Both bare and 10 % CVSB-modified carbon paste electrodes (CVSB-CPE) were employed to detect Pb(II), but the CVSB-CPE demonstrate significant electrocatalytic activity. Using an square wave anodic stripping voltammetry (SWASV) the sensor exhibited a linear response to Pb(II) ranging from 0.001 to 100 μM, with a limit of detection (LOD) of 0.57 nM. Additionally, repeatability tests showed a relative standard deviation (RSD) of 2.6 % over 17 consecutive measurements, while reproducibility across four independently prepared electrodes yielded a RSD of 1.05 %. The sensor also displayed high selectivity in the presence of potential interfering species, including Hg<sup>2+</sup>, K<sup>+</sup>, Cd<sup>2+</sup>, Na<sup>+</sup>, Ni<sup>2+</sup>, Fe<sup>3+</sup>, and Cu<sup>2+</sup>. The recovery tests conducted on factory effluent and hot spring water samples demonstrated a recovery ranging from 97.15 % to 113.49 % and 93.05 % to 108.01 %, respectively.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100928"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitivity-enhanced machine learning–assisted terahertz Plasmonic biosensor using hybrid 2D materials for tuberculosis detection 灵敏度增强的机器学习辅助太赫兹等离子体生物传感器，使用混合二维材料用于结核病检测

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2026-01-10 DOI: 10.1016/j.sbsr.2026.100964

K. Rejini , Humaira Nishat , P. Manikandan , P. Ashok , William Ochen

{"title":"Sensitivity-enhanced machine learning–assisted terahertz Plasmonic biosensor using hybrid 2D materials for tuberculosis detection","authors":"K. Rejini , Humaira Nishat , P. Manikandan , P. Ashok , William Ochen","doi":"10.1016/j.sbsr.2026.100964","DOIUrl":"10.1016/j.sbsr.2026.100964","url":null,"abstract":"<div><div>This work introduces, a machine learning-assisted terahertz metasurface biosensor that integrates graphene, gold, MXene and phosphorene within a single hybrid architecture for tuberculosis detection. Unlike conventional single- or dual-material plasmonic sensors, the proposed design exploits multi-material plasmonic hybridization to simultaneously achieve high sensitivity, angular robustness and tunable electromagnetic response. Finite element method (FEM) simulations in COMSOL Multiphysics are used to systematically optimize the sensor by varying the graphene chemical potential (0.1–0.9 eV), incident angle (0°–80°) and geometric parameters. The optimized design achieves a maximum sensitivity of 1000 GHz/RIU, figure of merit (FOM) of 14.286 RIU<sup>−1</sup>, quality factor of 10.014 and a detection limit of 0.022 RIU, indicating superior performance compared with conventional THz biosensors. Electromagnetic field analysis reveals strong field confinement and hybridized plasmonic modes within the 0.4–1.8 THz range, with a peak absorption of 76.935% at 80° incidence. A linear resonance frequency–refractive index relationship (R<sup>2</sup> = 0.98098) confirms reliable quantitative sensing. Furthermore, the incorporation of machine learning–assisted analysis, yielding an R<sup>2</sup> exceeding 90%, enhances predictive accuracy and robustness. The proposed architecture demonstrates high angular stability, compactness and tunability, establishing its novelty and suitability for point-of-care TB diagnostics and real-time biomedical sensing.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100964"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous and ultrasensitive detection of HBV DNA and RNA in CHB by utilizing Cas12a/Cas13a-based dual-target strategy 利用基于Cas12a/ cas13的双靶点策略同时超灵敏检测CHB中的HBV DNA和RNA

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2025-11-27 DOI: 10.1016/j.sbsr.2025.100925

Yuanyuan Li , Yuxin Liu , Lijuan Wu , Yueyue Chen , Mengchun Wang , Hongchun Luo , Juan Chen , Yuan Hu

{"title":"Simultaneous and ultrasensitive detection of HBV DNA and RNA in CHB by utilizing Cas12a/Cas13a-based dual-target strategy","authors":"Yuanyuan Li , Yuxin Liu , Lijuan Wu , Yueyue Chen , Mengchun Wang , Hongchun Luo , Juan Chen , Yuan Hu","doi":"10.1016/j.sbsr.2025.100925","DOIUrl":"10.1016/j.sbsr.2025.100925","url":null,"abstract":"<div><div>Simultaneous detection of low levels of serum HBV DNA plus RNA in HBV-infected patients receiving antiviral therapy is an effective strategy to evaluate the antiviral effect. In this research, we established an ultrasensitive dual PCR-CRISPR fluorescent assay (DPCFS) based on CRISPR-Cas12a/Cas13a system to simultaneous detection of low levels of HBV DNA and RNA. We optimized the DPCFS assay and evaluated the specificity and sensitivity employing the HBV plasmid. Clinical validation and comparison with commercial HBV DNA ultrasensitive qPCR assay or reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) were conducted using 46 serum samples of HBV-infected patients including 22 treatment-naïve and 24 nucleotide/nucleoside analogs (NAs)-treated patients. The limit of detection (LOD) for DPCFS platform was 5.8 copies/μL HBV DNA and 1.4 copies/μL HBV RNA, respectively, and the detection results could be directly visible to the naked eye under blue light without the need for any specialized instruments. Furthermore, clinical validation has 97.06 % sensitivity and 100 % specificity compared with commercial HBV DNA ultrasensitive qPCR assay. For serum HBV RNA detection, the sensitivity of DPCFS assay was 86.84 %, and the specificity was 87.5 % compared with RT-ddPCR. We developed a simultaneous and ultrasensitive detection of lower levels of HBV DNA and RNA in HBV-infected patients receiving antiviral therapy, which provide more accurate and simple strategy to evaluate the antiviral effect and treatment guidance.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100925"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric and time-resolved fluorescence lateral flow immunoassays for rapid screening of thrombosis and accurate quantification of D-dimer in human plasma 比色法和时间分辨荧光侧流免疫分析法用于快速筛选血栓形成和准确定量人血浆中d -二聚体

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2025-12-10 DOI: 10.1016/j.sbsr.2025.100940

Zhenyu Zhou , Xin'er Hu , Xinyi Xia , Meixiang Yu , Ningqi Xia , Jiayu Gu , Yan Cao , Hai Zhang

{"title":"Colorimetric and time-resolved fluorescence lateral flow immunoassays for rapid screening of thrombosis and accurate quantification of D-dimer in human plasma","authors":"Zhenyu Zhou , Xin'er Hu , Xinyi Xia , Meixiang Yu , Ningqi Xia , Jiayu Gu , Yan Cao , Hai Zhang","doi":"10.1016/j.sbsr.2025.100940","DOIUrl":"10.1016/j.sbsr.2025.100940","url":null,"abstract":"<div><div>D-dimer is a soluble fibrin degradation product, and usually serves as a biomarker for screening and diagnosis of venous thromboembolism (VTE). Immunoturbidimetry is the most commonly used detection method in clinical practice, but it cannot achieve point-of-care testing (POCT) and on-site detection. In this study, two lateral flow immunochromatographic assay (LFIA), colloidal gold lateral flow immunoassay (CG-LFIA) and time-resolved fluorescence microsphere lateral flow immunoassay (TRFM-LFIA), were established for rapid qualification and accurate quantification of D-dimer in human plasma. Both analytical methods were rapid, simple, and capable of on-site monitoring without complex sample pretreatment. The CG-LFIA achieved rapid qualitative assessment based on the visible color intensity of the strips and on-site quantitative detection through strip image scanning. Compared with CG-LFIA, the TRFM-LFIA can provide a wider dynamic range and superior sensitivity for D-dimer detection, although it does not allow for rapid qualification. After systematic optimization and rigorous methodological validation, the detection limit of CG-LFIA was 40 ng/mL with a dynamic range of 40–1280 ng/mL, while TRFM-LFIA had a lower detection limit of 6.1 ng/mL and a wider dynamic range of 10–2000 ng/mL. Both methods demonstrated excellent consistency in detecting D-dimer in human plasma in comparison with immunoturbidimetry. Therefore, CG-LFIA is more suitable for rapid on-site screening of clinical thrombosis patients preliminarily, while TRFM-LFIA is more appropriate for rapid and accurate POCT quantification of D-dimer in human plasma. Both methods will provide more convenient testing services for clinical thrombus screening and thrombus progression assessment.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100940"},"PeriodicalIF":4.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic platform combined with a dark field imaging system for quantification of E. coli contamination in water 微流控平台结合暗场成像系统定量测定水中大肠杆菌污染

IF 4.9

Sensing and Bio-Sensing Research Pub Date : 2026-02-01 Epub Date: 2025-12-16 DOI: 10.1016/j.sbsr.2025.100945

Anna Malec , Christoph Haiden , Georgios Kokkinis , Ioanna Giouroudi

引用次数: 0