Science of the Total Environment最新文献

{"title":"Nitrogen enhancement amplifies the precipitation-driven productivity allocation pattern in temperate and alpine grasslands.","authors":"Xiaoxiao Han, Qun Gang, Qianguang Liu","doi":"10.1016/j.scitotenv.2025.180483","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180483","url":null,"abstract":"<p><p>Grasslands constitute critical carbon sinks, vulnerable to altered precipitation and nitrogen deposition. While hyphae mediate ecosystem carbon allocation pathways, their role remains poorly quantified. We conducted multi-site manipulative experiments across China's temperate and alpine grasslands, quantifying carbon allocation to aboveground biomass, roots, and hyphae under precipitation change and nitrogen (N) enhancement. Our findings showed that along the precipitation gradient increasing, proportional carbon allocation to aboveground productivity (f_ANPP) increased, while allocations to root (f_BNPP) and hyphal (f_HNPP) productivity decreased. Moreover, productivity allocation in alpine grasslands was 1.30-1.50-fold more sensitive to precipitation changes than in temperate grasslands. N enhancement did not alter the precipitation-driven allocation pattern but amplified its sensitivity by 1.18∼-3.25-fold. And community traits, soil and root traits play an important mediating role in regulating productivity allocation through precipitation and nitrogen addition. These findings highlight the differential changes in the productivity allocation of different components driven by global change factors and emphasize the indispensable role of hyphae in regulating the stability of carbon sinks.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180483"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early response of soil bacterial communities to soil burn severity in pine and shrub vegetation after wildfire in temperate-humid climate.","authors":"Juan A Rubiolo, Felipe García-Oliva, José A Vega, Yunuen Tapia-Torres, José M Fernández-Alonso, Paulino Martínez, M Teresa Fontúrbel","doi":"10.1016/j.scitotenv.2025.180621","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180621","url":null,"abstract":"<p><p>Pine forests and shrublands worldwide are increasingly threatened by wildfires driven by global change, and those of temperate-humid ecosystems are no exception. Although the impact of soil bacterial of soil burn severity (SBS) on bacterial communities, has been examined in a number of studies, knowledge about their response across gradients of high SBS levels remains limited. In this study, we assessed the early effect (six weeks post-wildfire) of an SBS gradient, from moderate to extreme, previously defined, on bacterial communities in topsoil (0-2 cm) under adjacent pine and shrubland vegetation in northwestern Spain. Alpha diversity declined under moderate SBS, but no further decrease was observed at higher severity. Beta diversity increased, but community composition stabilized from moderate severity onwards, suggesting a threshold above which further compositional shifts are limited. Burned soils were enriched in Proteobacteria, Bacteroidetes, and Firmicutes, while Acidobacteria and Actinobacteria declined. Known pyrophilic genera (e.g. Massilia, Pedobacter), and others not previously reported with that character, were more abundant in burned soils. However, their increase did not extend beyond moderate SBS, reinforcing the notion of a compositional threshold. This stabilization may reflect the fire selection of fire-adapted communities' composition. Vegetation type, although a weaker driver than SBS, modulated bacterial responses: unburned pine and shrubland soils hosted similar communities that diverged after fire, with shrubland soils showing greater sensitivity. Soil pH, C, and C/N were strongly associated with bacterial composition. Our study provides evidence that moderate SBS may be sufficient to drive major shifts in soil bacterial communities, and that higher SBS levels do not necessarily lead to further changes, although confirmation through new studies is needed. The study findings may be useful for developing methods of accelerating the recovery of burned soils.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180621"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifunctional magnetic Fe/Fe₃O₄@Fe-N-C core-shell catalysts for external magnetic field-assisted enhancement of microbial fuel cell performance.","authors":"Hai-Xia Liao, Dong-Ni Ou, Fan-Li Xiao, En-Xi Ruan, Nan Li","doi":"10.1016/j.scitotenv.2025.180600","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180600","url":null,"abstract":"<p><p>Microbial fuel cells (MFCs) hold significant promise for sustainable energy generation and wastewater treatment. However, their practical performance is often constrained by sluggish cathodic oxygen reduction kinetics and limited anodic bioelectrochemical activity. Although external magnetic fields have been employed to enhance MFC performance, most studies focus on their effect on a single electrode. In this work, we synthesized a core-shell ferromagnetic catalyst, Fe/Fe₃O₄@Fe-N-C, using Fe₃O₄ as the magnetic core and a dopamine-assisted self-polymerization coating strategy. The catalyst exhibits strong magnetic responsiveness and excellent electrocatalytic activity. Under a 140 mT magnetic field, the half-wave potential (E_1/2) of the oxygen reduction reaction (ORR) increases to 0.719 V (vs. 0.705 V without the field), and the kinetic current density at 0.70 V increased by 1.6 times. When applied as both the cathode and anode catalyst in an MFC operating under a bipolar synchronous magnetic field, the system achieves a stable voltage output of 512 ± 14 mV and a maximum power density of 1156.8 ± 38.9 mW m^-2, maintains continuous operation for over 600 h. 16S rDNA sequencing reveals that the magnetic field enriches electroactive bacteria within the anode biofilm, optimizing the microbial community structure. These findings demonstrate that the synergistic integration of magnetic field and ferromagnetic materials can concurrently improve cathodic electrocatalysis and anodic microbial activity, offering a novel strategy for the design of high-performance MFCs.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180600"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping fluoride footprints: Fractionation, bioaccumulation, sources, and sophisticated risk assessment in El-Bardawil Lake, Sinai Peninsula, Egypt.","authors":"Naglaa F Soliman, Mamdouh A Fahmy, Mohamed A Abdelkhaleq, Samir M Nasr","doi":"10.1016/j.scitotenv.2025.180581","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180581","url":null,"abstract":"<p><p>Understanding fluoride (F) behavior in sediments and associated risks in hypersaline environments such as El-Bardawil Lake remains limited. This study investigated fluoride concentrations, distribution, sources, fractionation, mobility, and ecological and human risks to provide a comprehensive environmental assessment of this economically important lake. Sampling was conducted at twelve locations during 2023, targeting sediments, surface water, and two key bioindicator fish species, Sparus aurata and Mugil cephalus. Sequential extraction was applied to characterize sediment fluoride fractions: water-soluble (WsF), exchangeable (ExchF), Fe-Mn oxide-bound (Fe-MnF), organic-bound (OrgF), and residual (ResF). Environmental risk was assessed using contamination factors (CF), geo-accumulation indices (Igeo), individual contamination factors (ICF), risk assessment codes (RAC), bioavailability (BI), mobility indices (MI), availability indices (AI), and pollution coefficients (logK_d). Results indicated residual fluoride dominated sediment fractions (ResF: 64.51 %), followed by Fe-MnF (12.74 %), ExchF (10.59 %), OrgF (7.50 %), and WsF (4.66 %) forms. Sediment contamination was minimal (CF < 1; Igeo < 1; ICF < 0) with moderate ecological risk (RAC 11-15 %). Bioavailability and mobility (BI, MI: 0.1-0.4) and significant availability (AI > 0.4), facilitating transfer to the water column (logK_d < 3). Fluoride bioaccumulation primarily in liver tissues of both fish species. Although hazard quotient (HQ > 1) for Mugil cephalus suggests health risks for children via consumption, exposure via sediments and water (THQ < 1) remains negligible. Multivariate analyses (PCA, correlation matrix) revealed fluoride behavior is governed by sediment characteristics, organic matter content, and physicochemical parameters (pH, salinity). Fe-Mn oxides fraction as intermediate risks under reducing or high TOC conditions, while residual and organic fractions represent more stable or bioavailable pools. These findings provide critical insights for policymakers aiming to safeguard ecosystem health and ensure sustainable management of hypersaline lakes under increasing anthropogenic and climatic pressures.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180581"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge gaps and education opportunities on direct potable reuse: Interviews with customers of a large, southwestern United States water utility.","authors":"Amanda M Wilson, Mehedi Hasan, Yoonhee Jung, Lance Larkin, Yang Zhan, Dawn Morrison, Andrea Achilli","doi":"10.1016/j.scitotenv.2025.180603","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180603","url":null,"abstract":"<p><p>Water scarcity is a global public health threat that has increased urgency in implementing new sustainable practices to protect water supplies, such as the use of direct potable reuse, or \"advanced water purification (AWP)\". The study objective was to use interviews to characterize knowledge gaps and community outreach strategies to increase successful AWP implementation in an arid city in the southwestern United States. Through partnership with a water utility in an urbanized area of Arizona, 6000 individuals were emailed for invitation to participate in interviews. Interviews were conducted over Zoom and transcribed verbatim. Transcripts underwent inductive thematic analysis. Twenty-two individuals participated in interviews, and saturation of themes was reached. Five main themes emerged: 1) Conflation of filters with all treatment and the influence of residential technologies, 2) individual-level control over decisions to use advanced purified water, 3) desire for regulation, testing, and transparency about testing results, 4) concerns about specific chemicals, 5) educational resources to strengthen community engagement. Participants expressed lack of knowledge about how water is delivered to their residences by expressing the desire for opting in or out of system-wide treatment approaches. They also expressed wanting more support in interpreting testing results and having access to multiple outreach modalities. There is a growing body of evidence supporting increased outreach from utilities and governmental entities for water reuse adoption. This work provides insights into why the public may be in support or not of AWP and what information they need to form an opinion.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180603"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanogenic degradation of volatile fatty acids can be enhanced by using highly conductive materials or by forming electroactive biofilms on non-conductive materials.","authors":"Svetlana Shekhurdina, Elena Zhuravleva, Alexandra Laikova, Egor Andreev, Natalia Loiko, Artem Ivanenko, Nadezhda Popova, Emil Kryukov, Andrey Kovalev, Dmitriy Kovalev, Vivekanand Vivekanand, Andrei Eliseev, Yuriy Litti","doi":"10.1016/j.scitotenv.2025.180642","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180642","url":null,"abstract":"<p><p>To gain more insights into the impact of various additives on the stimulation of direct interspecies electron transfer (DIET), this study examined the anaerobic digestion (AD) performance of volatile fatty acids (VFAs) using different conductive (carbon felt (CF), stainless steel mesh (SM)) and non-conductive (polyester felt (PF), fiberglass mesh (FM)) carrier materials. The CH₄ yield in the PF and SM groups did not show a significant difference and was highest at 243.5 ± 6.0 and 224.7 ± 2.1 mL CH₄/g COD, respectively, representing an increase of 47 % and 35 %, respectively, compared to the control group (p < 0.001). The enhanced methanogenic activity of the SM group could be attributed to the highest conductivity of 11.1 ± 1.1 S/cm in SM and the associated DIET process. In contrast, the physicochemical properties of non-conductive PF, combined with the presence of specific functional groups, led to increased biofilm formation and a two-fold increase in extracellular polymeric substances. A significant improvement in the PF biofilm's conductivity and capacitance was observed, reaching 230 % and 20 % respectively, compared to the control group. Additionally, the presence of c-type cytochromes, a high abundance of pili-like structures, a diverse population of hydrogenotrophic methanogens, and the enrichment of syntrophic and potentially electroactive groups suggests the formation of an electroactive community on the PF. These findings imply that non-conductive materials with appropriate physicochemical properties may also foster the development of specific electroactive biofilms and enhance AD through the DIET pathway, similar to highly conductive materials.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180642"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of typhoid fever with floods under climate variability in 82 Belt and Road Initiative countries (2000-2021): A mixed-effects model and implications for water and sanitation infrastructure.","authors":"Joseph Kimuli Balikuddembe, Xiaolong Luo, Baofeng Di, Wen He, Bingwei Tian, Jan D Reinhardt, Josephine Namitala, Andreas Nienkötter, Tingting Yan, Jierui Li, Don Eliseo Lucero Prisno","doi":"10.1016/j.scitotenv.2025.180629","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180629","url":null,"abstract":"<p><p>Using four hierarchical mixed-effects models (MEMs), country-level data were analyzed to quantify longitudinal associations between floods and typhoid in 82 Belt and Road Initiative (BRI) countries (BRCs) under climate variability, adjusting for water, sanitation, hygiene (WASH), and population dynamics (2000-2021). Typhoid incidence (per 100,000) was the outcome variable, with flooding (occurrence, duration, and affected population), climate (including precipitation, temperature, and humidity), and covariates (drinking water, sanitation services, open defecation, and population) as predictors. Data sources included the Global Burden of Disease (typhoid), EM-DAT and FloodBase (floods), Copernicus ERA5 and Global Data Lab (climate), and World Bank and UNICEF (WASH and population). Model 2 (flood + climate variables) emerged as optimal for capturing flood-typhoid associations in BRCs, revealing climate-mediated pathways. Prolonged floods (>30 days) increased typhoid risk in vulnerable BRCs (like Bangladesh: β = 0.0021, *p < 0.001) by contaminating water and disrupting sanitation. Arid regions like the Middle East and North Africa showed null or inverse relationships due to short flood duration and pathogen die-off in dry conditions. Temperature consistently suppressed risk (like Kenya: β = -0.91, *p < 0.001), while precipitation had dual roles: dilution, which reduced risk in humid zones (like Vietnam: β = -0.0010, *p < 0.001) but increased concentrated contaminants in arid countries (like Chad: β = 0.0037, *p < 0.001). WASH covariates were often eclipsed by climate-flood interactions in Model 2. Riverine and flash floods posed higher risks than coastal floods. High-income BRCs exhibited negligible associations, attributable to robust infrastructure. Typhoid risk in most BRCs is predominantly mediated by climate-flood interactions, with Model 2 robustly capturing this pathway. Targeted BRI interventions, including climate- and flood-resilient WASH infrastructure and scaling China's \"Toilet Revolution\"-are critical for typhoid control in high-burden BRCs.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180629"},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accounting for albedo changes in carbon farming schemes.","authors":"Flora Desmet, Petra Sieber, Eric Ceschia, Jens Leifeld","doi":"10.1016/j.scitotenv.2025.180631","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180631","url":null,"abstract":"<p><p>Biogeophysical effects like changes in surface albedo are inherent to land-based climate mitigation projects, including carbon farming. Yet, these effects are typically overlooked in carbon farming schemes. Here we highlight the albedo-induced climate impacts of key carbon farming practices, which have been documented to be at the same order of magnitude as their greenhouse gas mitigation benefits. We discuss existing methods and data, as well as future opportunities and research needs to adequately quantify albedo-induced climate impacts and consider them in carbon farming schemes. Given that albedo effects can lead to cooler or warmer temperatures, carbon farming schemes should consider such biogeophysical co-benefits and trade-offs as part of their sustainability objectives related to climate change mitigation and adaptation.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180631"},"PeriodicalIF":8.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning for air quality prediction and data analysis: Review on recent advancements, challenges, and outlooks.","authors":"Manal Karmoude, Brenton Munhungewarwa, Isaiah Chiraira, Ryan Mckenzie, Jude Kong, Bevan Smith, Gelan Ayana, Nkosiphendule Njara, Thuso Mathaha, Mukesh Kumar, Bruce Mellado","doi":"10.1016/j.scitotenv.2025.180593","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180593","url":null,"abstract":"<p><p>Air quality is a critical determinant of human health, with severe consequences resulting from air pollution. The growing necessity for air quality monitoring has led to the adoption of IoT sensor networks, which provide real-time data for forecasting, issuing warnings, and informing public health interventions. In this context, machine learning (ML) algorithms have proven to be powerful tools for enhancing air quality prediction and addressing monitoring challenges. However, a comprehensive review compiling the research space of ML for air quality is seldom available. This review analyzes over 70 recent studies that apply ML techniques to air quality monitoring, categorizing them based on the type of learning approach employed, with a focus on identifying the most effective algorithms in each category. The findings demonstrate that ensemble models such as Random Forest (RF) and Extreme Gradient Boosting (XGBoost) consistently achieve high accuracy in structured datasets, while deep learning (DL) approaches like Long Short-Term Memory (LSTM) and Convolutional Neural Networks (CNN) excel in capturing temporal dependencies and spatial patterns in pollution forecasting. Unsupervised approaches like clustering and anomaly detection effectively enhance data quality and sensor calibration, whereas reinforcement learning shows promise in adaptive control scenarios, despite challenges related to computational intensity and interpretability. This review is highly significant, offering valuable insights for policymakers and researchers in developing strategies to mitigate air pollution and improve public health using advanced ML techniques.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180593"},"PeriodicalIF":8.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral genetic diversity in surface and groundwater at a non-operational dumpsite and its surrounding neighborhood.","authors":"Sara Kahwage, Natália Maria Lanzarini, Bruna Barbosa de Paula, Enrico Mendes Saggioro, Anne Torres de Faro Motta, Camille Ferreira Mannarino, Marize Pereira Miagostovich","doi":"10.1016/j.scitotenv.2025.180604","DOIUrl":"https://doi.org/10.1016/j.scitotenv.2025.180604","url":null,"abstract":"<p><p>Inappropriate municipal solid waste disposal areas lack waterproof base liners, drainage systems, treatment of waste degradation by-products, mass coverages, and vector control. Leachate is a by-product derived from water percolation through solid waste disposal sites, comprising an environmental and health contamination source when not adequately collected and treated. This study aimed to describe surface and groundwater viral diversity in a dumpsite area non-operational for over a decade and its surrounding neighborhood in the metropolitan Rio de Janeiro region employing shotgun metagenomic analysis. Between May 2021 and March 2023, 100 leachate samples (2 L each) were collected from 21 sampling points and subsequently organized into five pooled samples. Nucleic acids were extracted employing the Biopur Nucleo-mag Virus kit, followed by purification with AMpure XP beads and quantification via Qubit 4.0, Next-Generation Sequencing was performed on a NextSeq 2000 sequencer (Illumina, USA). Sequencing analysis identified 578 operational taxonomic units, of which 312 (54 %) were classified into 46 viral families, while 266 (46 %) remained unclassified, the Microviridae family was the most abundant. Viral sequences linked to pathogenic hosts were detected, including canine circovirus. Comparable viral family profiles were observed between the dumpsite and the residential area suggesting that some of the shared viral families may be influenced by groundwater flow originating from the closed dumpsite. Although irregular land use hinders precise attribution of contamination sources, the detection of potentially pathogenic viruses in waters intended for human use raises significant public health concerns. These findings support long-term virome-based surveillance of groundwater near closed dumpsite.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"180604"},"PeriodicalIF":8.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}