Science of the Total Environment最新文献

{"title":"Mechanistic insights into oxychar’ s role in mitigating ammonia volatilization from fertilised alkaline soils","authors":"Zeyu Zhu ,&nbsp;Wei Liu ,&nbsp;Jing Hu ,&nbsp;Kexin Zhao ,&nbsp;Bo Niu ,&nbsp;Shutao Wang ,&nbsp;Chaoyu Li ,&nbsp;Abbas Z. Kouzani ,&nbsp;Bing Han","doi":"10.1016/j.scitotenv.2025.179502","DOIUrl":"10.1016/j.scitotenv.2025.179502","url":null,"abstract":"<div><div>Mitigating ammonia (NH₃) volatilization from fertilised alkaline soils is crucial for promoting environmental sustainability and safeguarding public health. In this study, “oxychar” was prepared from an agricultural waste via a low-temperature partial-oxidation (LTPO) process under oxygen-sufficient environment. The optimum modification temperature was 220 °C for a duration of 3 h, yielding a maximum NH₃ uptake of 76.4 mg/g by oxychar. The enhanced adsorption capacity and stability are attributed to the increased surface oxygen-containing functional groups (OCFGs) that act as adsorption sites, as well as the presence of ultra-micropores that facilitate effective trapping. When applying optimised oxychar to 21-day incubation studies, it is found that cumulative NH₃ volatilization is reduced by 76.3 %–91.6 % with oxychar application rates of 1 %–3 %, compared to urea treatments. Soil property and microbial community analyses further indicate that oxychar promotes the hydrolysis of urea and the reduction of nitrate to ammonium by dissimilation (DNRA) processes while inhibiting nitrification by modulating microbial communities, resulting in higher ammonium and lower nitrate levels in soil compared to urea treatment. However, the enriched ammonium cannot volatilize freely due to the chemisorption of ammonium and NH₃ by oxychar via ionic and covalent bonds, respectively. Introducing oxychar to soil could be a promising approach to mitigate NH₃ volatilization from fertilised soil to ensure food security and environmental sustainability.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179502"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulation in the Guafo Mouth: The gateway to northern Patagonia","authors":"Lauren Ross ,&nbsp;Iván Pérez-Santos ,&nbsp;Pamela Linford ,&nbsp;Patricio A. Díaz","doi":"10.1016/j.scitotenv.2025.179512","DOIUrl":"10.1016/j.scitotenv.2025.179512","url":null,"abstract":"<div><div>Circulation and exchange are important factors governing material transport in all coastal areas, including Chilean Patagonia, a region that boasts one of the largest aquaculture industries in the world and provides habitat for several protected marine mammal species. Linkages between coastline shape and forces of hydraulic conditions, such as tides, wind, sea level gradients, and river discharge are thereby important considerations when understanding constituent concentrations and transport related to water quality issues, which threaten aquaculture activities and endanger wildlife and humans. The primary connection between the Pacific Ocean and the fjords of Chilean Patagonia is through the Guafo Mouth, however the structure and drivers of circulation, and its impact on water quality, has yet to be studied. The research presented here characterizes circulation patterns and variability in the Guafo Mouth to help inform ocean-fjord exchange. Measurements of current velocities, salinity, temperature, dissolved oxygen, and nutrients were collected from 2020 to 2023 in the channel. These measurements, in addition to a numerical model of the study area and reanalysis data, provide parallel evidence of subtidal flows driven by the competition between barotropic and baroclinic pressure gradients. Depending on the sign of the barotropic pressure gradient, the barotropic flow either augments (when negative) or opposes (when positive) the circulation produced by horizontal density gradients. When the near-bottom subtidal inflow enhances, offshore waters with high nutrient content and low dissolved oxygen can be advected in-channel. This occurred at periods between ∼40 and 70 d concurrent with poleward pulses of the Peru-Chile Undercurrent that is known to transport nutrient rich and low dissolved oxygen waters southward off the coast of Chile. These findings imply that ocean-fjord exchange through the Guafo Mouth, which is controlled by water level gradients from the Pacific Ocean to the inner fjords, directly influences the import of dissolved oxygen and nutrients.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179512"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic signatures of pathogen suppression effect of Baltic eelgrass meadows in surrounding seawater","authors":"Caroline Utermann-Thüsing ,&nbsp;Daniel Mendez ,&nbsp;Paolo Stincone ,&nbsp;Daniel Petras ,&nbsp;Deniz Tasdemir","doi":"10.1016/j.scitotenv.2025.179518","DOIUrl":"10.1016/j.scitotenv.2025.179518","url":null,"abstract":"<div><div>Organic molecules exuded into water column by marine organisms represent a significant portion of marine dissolved organic matter (DOM) that modulates biochemical interactions. Secreted allelochemicals have been suggested to be involved in regulation of pathogen abundance in seagrass meadows, however, seagrass exometabolome has remained unstudied. We aimed to identify seagrass exometabolites, within and outside meadows, and explore their potential involvement in pathogen suppression under varying environmental conditions. We collected seawater (SW) samples from eelgrass (<em>Zostera marina</em>)-vegetated (V) and non-vegetated (NV) areas across 5 locations spanning 270 km of coastline along the German Baltic Sea. Comparative LC-MS/MS-based untargeted computational metabolomics combined with statistical analyses and machine learning tools were employed to pinpoint (exo)metabolomic signatures of eelgrass leaves. Simultaneously, we measured abiotic parameters and the abundance of three common pathogenic taxa in seawater, and investigated spatiotemporal variations. Here we show the correlation of pathogen biomass and eelgrass pathogen reduction effect with increasing seawater temperature, eutrophication and anthropogenic influences. Exometabolomics studies revealed that eelgrass exudates contributed significantly to overall seawater DOM at molecular level, while SW overlying eelgrass meadows contained many chemical features unique to the eelgrass leaf metabolome. We identified four flavone aglycones as key biomarkers distinguishing SW-V and SW-NV samples. Their drastically increased concentrations correlated with the lowest pathogen biomass, suggesting their role in pathogen regulation. These combined analytical and microbiological approaches indicate that flavones are defensive allelochemicals released into eelgrass meadows upon environmental stress and serve as potential bioindicators of eelgrass’ sanitation effect.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179518"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residential indoor temperatures and health: A scoping review of observational studies","authors":"Janelle R. Edwards ,&nbsp;Anneclaire J. De Roos ,&nbsp;Chima C. Hampo ,&nbsp;Wanyu Huang ,&nbsp;Emily Lincoln ,&nbsp;Simi Hoque ,&nbsp;Leah H. Schinasi","doi":"10.1016/j.scitotenv.2025.179377","DOIUrl":"10.1016/j.scitotenv.2025.179377","url":null,"abstract":"<div><div>Adults spend most of their time indoors, especially in higher income countries. Indoor temperature exposures can vary substantially across households, even within a single geographic area. It is therefore critical to understand links between indoor temperature exposures and health or well-being outcomes, and to understand safe maximum indoor residential temperature thresholds that support health, well-being, and comfort. We systematically identified peer-reviewed, observational studies that quantified associations between residential indoor temperatures and mortality/morbidity outcomes. We extracted information on study location; population, health or well-being outcomes; indoor temperature exposure assessment methods; and, when available, empirically quantified safe maximum indoor temperature thresholds. In total, 29 papers were included in the review. The studies were conducted in the following continents: North America (<em>N</em> = 10), Europe (<em>N</em> = 5), Asia (<em>N</em> = 9), Australia (<em>N</em> = 4), and Africa (N = 1). The most common outcomes were cardiovascular morbidity (N = 10) and respiratory morbidity (<em>N</em> = 8) and thermal comfort (<em>N</em> = 9). Exposure assessment methods included data sensors, thermometers, data-driven models, and energy-based simulations. Despite variation in exposure assessment methods and outcomes assessed, results predominately suggested that warmer indoor temperatures were associated with adverse health or well-being outcomes, although in a handful of studies, associations were either null or in the unexpected, protective direction. Empirically identified safe thresholds for indoor temperature ranged from 18 °C to 35 °C and varied according to outcome. Results from this review may be used to inform the design of future studies of associations between indoor temperatures and morbidity or mortality outcomes.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179377"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaccumulation of decabromodiphenylethane (DBDPE) in aquatic and terrestrial biota in the global environment and its toxicological significance assessed by chemical activity","authors":"Kate M. Fremlin ,&nbsp;Nicole Berg ,&nbsp;Frank A.P.C. Gobas","doi":"10.1016/j.scitotenv.2025.179368","DOIUrl":"10.1016/j.scitotenv.2025.179368","url":null,"abstract":"<div><div>Many industrial substances cannot be assessed for their bioaccumulation potential using the octanol-water partition coefficient (<em>K</em>_OW), bioconcentration factor (BCF), or bioaccumulation factor (BAF) criteria typically used in the United Nations Stockholm Convention for Persistent Organic Pollutants (POPs) and in regulations and laws of many countries including Canada, the European Union (EU), the United States (US), and Japan. This presents an impediment to an efficient, effective, and expedient environmental assessment of many substances. However, the Stockholm Convention includes a second criterion for bioaccumulation assessments that is focussed on “<em>Evidence that a chemical presents other reasons for concern, such as high bio-accumulation in other species, high toxicity, or ecotoxicity</em>.” But this criterion has received little attention; is not recognized or used in national regulations; and lacks a framework for evaluation. This study investigates the application of this second criterion to (i) assess the bioaccumulation potential of difficult-to-test and difficult-to-evaluate substances that cannot be adequately evaluated with <em>K</em>_OW, BCF, or BAF due to very low aqueous solubility, ionization poor solubility in lipids, large molecular size, or other reasons; and (ii) present and test a framework for bioaccumulation assessments that aims to determine whether substances bioaccumulate to levels of concern. The framework is tested using evidence of bioaccumulation for decabromodiphenyl ethane (DBDPE; CAS: 84852-53-9), which is a super-hydrophobic substance that is difficult to test in aqueous and dietary bioaccumulation tests. To evaluate DBDPE with this framework, we compiled bioaccumulation and toxicological data from the published literature, evaluated the exposure concentration data for quality, and applied a chemical activity-based evaluation. Our results indicate that based on available information to date, DBDPE does not appear to bioaccumulate to levels of concern that would affect environmental health at a global or local scale. The results also suggest that our evaluative framework may be a useful alternative for other super-hydrophobic and/or ionizing substances like perfluoroalkyl and polyfluoroalkyl substances and/or aromatic brominated flame retardants that cannot be assessed for bioaccumulation potential based on <em>K</em>_OW, BCF, or BAF.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179368"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activated carbon-based hot water-assisted backwashing system for active disinfection of Chironomus riparius larvae","authors":"Rajendra Singh ,&nbsp;Chae-seung Lim ,&nbsp;Sung Soo Park ,&nbsp;Hayoung Kim ,&nbsp;Woo Hyoung Lee ,&nbsp;Sanghwa Park ,&nbsp;Keugtae Kim","doi":"10.1016/j.scitotenv.2025.179475","DOIUrl":"10.1016/j.scitotenv.2025.179475","url":null,"abstract":"<div><div>The presence of midge larvae in tap water is a critical issue for the environmental health of water sources, as well as for water quality and safety. Although methods such as ozone treatment, coagulation and chemical applications have been attempted as primary removal methods, they have not yet succeeded in completely eradicating larvae. This study identified the use of hot water for filter backwashing as an effective method for fully eliminating larvae in water treatment facilities. Despite its apparent simplicity, this approach has not been previously explored for larval disinfection. We developed a bench-scale system that utilizes activated carbon and hot water-assisted backwashing. Our research shows that filter backwashing can achieve total disinfection of <em>Chironomus riparius</em> larvae at 45 °C in just 3 min. Furthermore, a cost assessment was conducted to determine the cost efficiency of the designed system for implementation in water treatment facilities. An economic evaluation based on the Bucheon Water Treatment Plant (with a daily flow capacity of 600,000 m³) revealed an additional cost of $3.65 per 1000 m³ of water to ensure effective larval control. This study provides an efficient and environmentally sustainable solution for removing midge larvae from water treatment facilities.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179475"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ExpoPath: A method for identifying and annotating exposure pathways from chemical co-occurrence networks","authors":"Michael A. Zurek-Ost ,&nbsp;Katherine A. Phillips ,&nbsp;Antony J. Williams ,&nbsp;Adam Edelman-Muñoz ,&nbsp;Nathaniel Charest ,&nbsp;Sakshi Handa ,&nbsp;Kristin K. Isaacs","doi":"10.1016/j.scitotenv.2025.179465","DOIUrl":"10.1016/j.scitotenv.2025.179465","url":null,"abstract":"<div><div>Improving risk evaluation for environmental and human health is of paramount concern for the U.S. Environmental Protection Agency (EPA). This includes the identification and assessment of chemical transport from commercial and industrial sources to environmental and ecological media, where repeated patterns are often categorized as exposure pathways. Utilizing network analysis techniques paired with graph machine learning tools allows for the construction and analysis of a global chemical co-occurrence network with which to identify sets of overlapping or distinct communities that represent likely exposure pathways. Data from several chemical source databases were aggregated and used to generate a chemical co-occurrence network that encoded linkages between source categories and environmental and receptor categories within the EPA's Multimedia Monitoring Database (MMDB). Multiple algorithms were used to detect communities of chemicals within this network, while enrichment of the resulting communities based on presence-in-media information, physicochemical properties, and functional use information helped to annotate likely exposure pathways. This research identified communities of chemicals associated with various pharmaceutical, consumer, pesticide, and persistent chemical pathways. This novel approach to the study of chemical co-occurrence demonstrates the applicability of network analyses and graph machine learning methods for identifying empirical patterns of connectivity within the domain of exposure science.</div></div><div><h3>Synopsis</h3><div>Network analysis and community detection algorithms help reveal linkages among environmental monitoring data and chemical sources while providing supporting evidence for empirically derived exposure pathways.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179465"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative approach for assessing risks and benefits to the supply of ecosystem services in response to human activities","authors":"Dries Lorré ,&nbsp;Sara Vandamme ,&nbsp;Ulrike Braeckman ,&nbsp;Colin R. Janssen ,&nbsp;Jana Asselman","doi":"10.1016/j.scitotenv.2025.179504","DOIUrl":"10.1016/j.scitotenv.2025.179504","url":null,"abstract":"<div><div>Escalating human activities threaten ecosystems and the benefits they provide, known as ecosystem services (ES). Despite the recognized importance of ES for both ecological health and human well-being, integrated methods for evaluating ES within decision-making frameworks remain limited. Current environmental assessments, such as ecological risk assessment (ERA), typically focus on risks to specific endpoints such as survival, growth and reproduction of test species without capturing broader ecosystem risks and benefits. This study introduces a novel method designed to quantitatively assess risks and benefits to ES supply by integrating ES as assessment endpoints within ERA. Using cumulative distribution functions, we establish risk and benefit thresholds and calculate the probability and magnitude of exceeding these following human interventions. The method was tested by quantifying risk and benefit metrics for a regulating ES, waste remediation, in three marine offshore case studies: an existing offshore wind farm, a hypothetical mussel longline culture, and a multi-use scenario combining both. The results enabled detailed comparisons of the probability and magnitude of creating risks and providing benefits across scenarios, demonstrating the utility of cumulative distribution functions for both visualizing and quantifying risks and benefits to ES supply. This generic and broadly applicable method can evaluate ES trade-offs regardless of the ecosystem under study, providing a valuable tool to operationalize the integration of ES into decision-making and environmental management frameworks.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179504"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of phloem transport of foliar-absorbed Cd in wheat","authors":"Liping Li ,&nbsp;Liping Wang ,&nbsp;James A. Ippolito ,&nbsp;Weiqin Xing ,&nbsp;Li Ma ,&nbsp;Kunyan Qiu ,&nbsp;Junxiao Jiang","doi":"10.1016/j.scitotenv.2025.179496","DOIUrl":"10.1016/j.scitotenv.2025.179496","url":null,"abstract":"<div><div>The transport pathway of foliar absorbed Cd in wheat has not been clarified. Hydroponically raised wheat seedling stems were steam girdled to disrupt phloem transport, then the leaves were exposed to Cd solutions (40 or 60 mg L⁻¹) containing 20 mg L⁻¹ Rb (a phloem transport indicator) for 16 h. Plants were harvested 7 days later and divided into roots and leaves, while the stem was divided into two parts above and below the girdling location, with phloem sap collected from the upper part. Samples were analyzed for Cd, Rb, Cu, Zn and Mn concentrations; root glutathione concentration was also analyzed to investigate Cd transport and its effect on other metals. Girdling increased leaf Cd concentrations by &gt;2 folds, reduced root Cd and Rb concentrations by ∼50 %, reduced root glutathione concentration by 21.4 %, and phloem sap Rb concentrations of the girdled plants were 30.8–63.1 % greater than the ungirdled treatments. Root to leaf Cd concentration ratios were in the range of 0.064–0.443, and girdled treatments had lower values than the ungirdled treatments. Negative correlations were found between root Cd and leaf Mn and Cu concentrations in the ungirdled treatments, but not in the girdled treatments. These results indicate that phloem transport plays an important role in the transport of leaf-absorbed Cd in wheat, with leaf absorbed Cd having high mobility. These findings are important for discerning Cd uptake and transport in plants found living in Cd-contaminated conditions.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179496"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial communities and fatty acid markers across acidification and eutrophication extremes in a river influenced by mining activities","authors":"Germán A. Kopprio ,&nbsp;Michael Hupfer ,&nbsp;Martin Graeve ,&nbsp;Susan Mbedi ,&nbsp;Sarah Sparmann ,&nbsp;Tobias Goldhammer","doi":"10.1016/j.scitotenv.2025.179473","DOIUrl":"10.1016/j.scitotenv.2025.179473","url":null,"abstract":"<div><div>Microbial communities in combination with fatty acid and isotopic markers were studied seasonally to assess the effects of acid mine drainage (AMD) and nutrient loads in the Spree river. Negative values of δ¹⁵N, the bacterial and detrital markers 18: 1(n-7) and 18:1(n-9), pH values ∼3 and bacteria of the genera <em>Ferrovum</em>, <em>Thiomonas</em>, <em>Acidocella</em>, <em>Acidiphilum</em>, <em>Syderoxydans</em> and <em>Galionella</em> were characteristic of the AMD extreme. Potential iron-oxidizers may produce ferric ions and their precipitates may influence biogeochemical processes, while potential sulfur-oxidizers may contribute to elevated sulphate concentrations and challenge drinking water production in the Spree catchment. In this river, eutrophication was linked with polyunsaturated fatty acids (PUFA) enrichment and not with PUFA depletion as occurs in other freshwater systems. Elevated concentrations and proportions of PUFA as well as higher relative sequence abundance of cyanobacteria were characteristic of the highly eutrophic station, particularly during the phytoplankton bloom. The 18:5(n-3) from flagellates or dinoflagellates may indicate lipid anabolism and trophic upgrading processes. The dominance of the classes Bacteroidia, Gammaproteobacteria and Actinobacteria suggested eutrophic and changing hydrological conditions in the river. The microbial communities were better markers of seasonality than the biogeochemical markers and their combination offers an excellent resolution for the study of the ecology and biogeochemistry of water courses. The expected decreased runoff under climate-driven scenarios may worsen the AMD pollution and eutrophication problems and signify a considerable challenge for water management.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"979 ","pages":"Article 179473"},"PeriodicalIF":8.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}