Science of the Total Environment最新文献

{"title":"Cumulative effects of fire in the Fraser River basin on freshwater quality and implications for the Salish Sea","authors":"Emily Brown ,&nbsp;Brian P.V. Hunt","doi":"10.1016/j.scitotenv.2025.179416","DOIUrl":"10.1016/j.scitotenv.2025.179416","url":null,"abstract":"<div><div>The movement of water from land to the ocean serves as a major biogeochemical link between terrestrial and marine systems, through which terrestrial disturbances can impact freshwater quality and coastal oceans. Wildfire is a major terrestrial disturbance, however its influence on water quality in large freshwater systems and the ocean is understudied. As anthropogenic pressures change fire regimes globally, it is important that this connection is better understood. The Fraser River's basin has significant wildfire history, and the Fraser River has major influence on its receiving waters of the Salish Sea, making this an ideal system in which to investigate the influence of fire on water quality from freshwater to the ocean. This study assessed cumulative impacts of wildfire on Fraser River water quality using historical water quality and fire data. Wildfire in the Fraser River basin explained up to 16.3 % of variance in water quality, and fires burning closer to major waterways had immediate influence on water quality, while farther away wildfires had a delayed influence on water quality. For water quality variables of particular importance in the Salish Sea, wildfire was linked to short term decreases in the riverine concentrations of some constituents, and delayed increases in the concentrations of nearly all assessed water quality variables. These findings have implications for coastal ocean productivity and contamination, and identify fire as an important agent of biogeochemical cycling between land and ocean.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179416"},"PeriodicalIF":8.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel seaweed detection image processing and validation framework: A pragmatic study on natural seaweed beds along North-West Coast of India","authors":"D. Bhavana ,&nbsp;A. Rooha Tej ,&nbsp;G. Jyothi Swaroop ,&nbsp;Ramesh Kumar Mojjada ,&nbsp;Abdul Azeez Pokkathappada ,&nbsp;Suresh Kumar Mojjada ,&nbsp;Swathi Lekshmi Perumal Sundaram ,&nbsp;Aarsha Subramanian ,&nbsp;Prachi Siddharth Bagde ,&nbsp;Mayur Shivdas Tade ,&nbsp;T.S. Ramshad ,&nbsp;Dhanush Janarthanan ,&nbsp;Muktha Menon ,&nbsp;Suresh Vettath Raghavan ,&nbsp;Grinson George ,&nbsp;D. Divu ,&nbsp;Devanaboyina Venkata Ratnam","doi":"10.1016/j.scitotenv.2025.179296","DOIUrl":"10.1016/j.scitotenv.2025.179296","url":null,"abstract":"<div><div>Seaweeds play a pivotal role in global ecology contributing significantly to biodiversity conservation and coastal habitat of marine ecosystems. In particular, natural seaweed beds are increasingly vulnerable to climate change and commercial exploitation, necessitate the effective monitoring, especially given the extensive coastlines. Thus, this study presents a novel framework that integrates remote sensing, image processing techniques, and on-site validation methods to standardize indices for seaweed cover changes and abundance detection across three selected natural coastal seaweed beds along the biodiversity-rich North-West Coast of India. We introduce a novel in-situ validation method to assess seaweed abundance while standardizing three remote sensing indices i.e. the Normalized Difference Vegetation Index (NDVI), the Floating Algae Index (FAI), and the Seaweed Enhancing Index (SEI). By correlating ground-truth measurements of seaweed biomass with values derived from remote sensing indices, we enable detailed estimations of both presence and abundance. Our findings reveal that the natural seaweed beds along the Veraval coast exhibit the highest levels of vegetation cover, followed by other selected sites at Kelwa and Porbandar, with robust correlations observed across all indices. Notably, the SEI demonstrated superior accuracy in identifying seaweed habitats compared to NDVI and FAI. Ground-truth validation substantiates the reliability of our results, signifying positive correlations between the index outputs and actual seaweed abundance. Also, this study establishes a robust framework for future research by introducing indices standardization methodologies for remote sensing and image processing of seaweed habitats. By demonstrating the efficacy of in-situ validation and grid-based assessments, we have effectively quantified seaweed density and distribution. Furthermore, the integration of advanced remote sensing data from Landsat-8 not only facilitates long-term monitoring but also provides a valuable baseline for spatio-temporal analyses of seaweed habitat dynamics.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179296"},"PeriodicalIF":8.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species invasion has a larger impact on stickleback reproduction than warming of breeding habitat","authors":"Tawfiqur Rahman,&nbsp;Ulrika Candolin","doi":"10.1016/j.scitotenv.2025.179447","DOIUrl":"10.1016/j.scitotenv.2025.179447","url":null,"abstract":"<div><div>The invasion of foreign species into ecosystems is a growing human-induced problem. Global warming is expected to magnify the problem by facilitating invasions and amplifying the ecological impact of invaders. We investigated if rising water temperature influences the impact of an invading shrimp <em>Palaemon elegans</em> on the reproductive success of a native fish, the threespine stickleback <em>Gasterosteus aculeatus</em>. Using a 2 × 2 factorial design, we found the shrimp to reduce the probability that stickleback males built a nest, defended it against a perceived intruder, courted females and gained matings. This reduced the number of offspring they produced, independently of temperature. Males lost more weight in the presence of the shrimp, which indicates that the shrimp can reduce the number of breeding cycles that males can complete. Thus, the shrimp has both direct negative effects and the potential for indirect delayed effects. The impact of the shrimp on stickleback reproduction overrode that of warming, most likely because the stickleback has adapted to temperature fluctuations in shallow coastal waters, but not to a high abundance of the non-native shrimp. Interestingly, the shrimp did not reduce egg hatching success, which indicates that the stickleback overestimates the risk posed by the invader. Refraining from reproduction in the presence of shrimp may have been an adaptive strategy in the past, but not in the current environment where shrimp-free habitats may no longer be found. Thus, the past reaction norm – to avoid nesting in the presence of shrimp – may have become an ‘evolutionary trap’. These results emphasise the immediate threat that invading species can pose, which can be larger than the impact of warming, and stresses the importance of controlling their invasions.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179447"},"PeriodicalIF":8.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis of factors that influence the spatial pattern of faecal matter flow in unsewered cities","authors":"M Sufia Sultana ,&nbsp;Toby Waine ,&nbsp;Niamul Bari ,&nbsp;Sean Tyrrel","doi":"10.1016/j.scitotenv.2025.179440","DOIUrl":"10.1016/j.scitotenv.2025.179440","url":null,"abstract":"<div><div>The management of sanitation systems in unsewered cities in low and middle income countries is a critical issue, yet it is unclear where the risk hotspots are and where interventions should be focused. This study utilised a prototype model, developed by the authors, to map the spatial pattern of faecal flow in Rajshahi city, a secondary city in northwest Bangladesh with a population around a million. This city serves as a representative example of 60 such secondary cities in Bangladesh and hundreds more in the economically developing region in Asia, Africa and Latin America. The model relies on assumptions that carry significant uncertainties; hence, the study employed a sensitivity analysis with multiple plausible scenarios to characterise these uncertainties, aiming to identify ways to improve the model further. Five major influencing factors on the spatial pattern of faecal flow were identified: the emptying of septic tanks, the use of soak pits, and sludge removal from drains, variations in faecal matter production by building types, and the presence or absence of toilets. These factors were shown to collectively have a significant impact (almost 50 % changed) on the model outcome, depending upon the assumptions made. The study offers insights that will guide future data collection efforts by emphasising the need to understand these specific influencing factors and their spatial pattern. Consequently, this research has broader implications for urban sanitation management as well as associated public health research like wastewater surveillance, risk assessment, and disease dynamics in similar urban settings, offering insights into areas of uncertainty that need to be addressed in future modelling efforts.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179440"},"PeriodicalIF":8.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of SO2 and CO2 emission rates from coal-fired power plants in the Korean peninsula via airborne measurements","authors":"Jeonghwan Kim ,&nbsp;Gangwoong Lee ,&nbsp;Jongbyeok Jun ,&nbsp;Beom-Keun Seo ,&nbsp;Yongjoo Choi","doi":"10.1016/j.scitotenv.2025.179430","DOIUrl":"10.1016/j.scitotenv.2025.179430","url":null,"abstract":"<div><div>A reliable ensemble averaging method was developed to quantify sulfur dioxide (SO₂) and carbon dioxide (CO₂) emission rates from the Taean and Dangjin power plants in South Korea. This method integrated mass balance and Gaussian footprint approaches while accounting for individual uncertainties and deviations arising from distinct modeling assumptions and measurement variability. Eighteen representative spiral flights in 2022 (9 cases) and 2023 (9 cases) were conducted to evaluate emission rates and revealed several optimal conditions for achieving accurate quantification, including a small spiral radius with a fine vertical resolution under unstable atmospheric conditions. Validation of the estimated SO₂ emission rate revealed comparable correlation coefficients (<em>R</em> &gt; 0.72) between the two methods and the real time automatic telemonitoring system (CleanSYS). The ensemble averaging method mitigated the sensitivity of the Gaussian footprint to the effects of meteorological conditions and high uncertainty in the mass balance, which resulted in an improved correlation of the estimated SO₂ emission rate with that measured by the CleanSYS (<em>R</em> &gt; 0.78). When the same approach was applied, the CO₂ emission estimates from both methods showed a high correlation (<em>R</em> &gt; 0.78) and confirmed the robustness of our ensemble averaging method. Although there was no significant difference between monthly electricity production in 2022 (October and November) and 2023 (May, October and November), the SO₂ emission rates decreased by 37 % and 29 % compared with the ensemble averaging method and CleanSYS, respectively; however, CO₂ emission rates increased by approximately 62 % at Taean and 83 % at Dangjin. This could be attributed to the use of carbon-intensive fuel sources, more intensive operations during research flight, and the desulfurization process, which aimed to reduce SO₂ emissions and release CO₂ as a byproduct. This study highlights the broad application of our ensemble averaging method for emission monitoring and regulatory compliance, particularly for CO₂, when real-time emission monitoring systems are absent.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179430"},"PeriodicalIF":8.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of mobile resistance elements in extended-spectrum β-lactamase producing gram-negative bacteria from aquatic environment","authors":"Yang Zhong ,&nbsp;Jocelyn Qi-Min Teo ,&nbsp;Siyao Guo ,&nbsp;Joergen Schlundt ,&nbsp;Andrea Lay-Hoon Kwa ,&nbsp;Rick Twee-Hee Ong","doi":"10.1016/j.scitotenv.2025.179353","DOIUrl":"10.1016/j.scitotenv.2025.179353","url":null,"abstract":"<div><div>Extended-spectrum β-lactamase producing (ESBL) bacteria from aquatic environments can pose potential threats to public health due to their capability of spreading antimicrobial resistance (AMR) genes through mobile genetic elements (MGEs), such as plasmids, insertion sequences (ISs), transposons, and integrons. Currently, there is no policy for routine monitoring of AMR genes in aquatic environments and their roles in transmission are therefore unknown. Previous metagenomic and PCR-based culture-independent approaches are limited in recovering AMR resistant aquatic bacteria isolates and the data resolution generated are not able to provide detailed genetic comparison with known human pathogens particularly for determining genetic islands harbouring AMR genes. To address these gaps, we thus investigated the genetic profiles of ESBL-producing gram-negative aquatic bacteria found from water body sites within Singapore, examining the AMR genes carried and their associated MGEs. In total, 16 ESBL-producing gram-negative bacteria were identified, of which 8 were <em>Escherichia coli</em>, 3 <em>Klebsiella pneumoniae</em>, and 5 <em>Aeromonas spp.</em> Whole genome sequencing (WGS) analysis revealed the presence of 12 distinct classes of AMR genes, including 16 distinct variants of β-lactamase, of which <em>bla</em>_CTX-M was the dominant beta-lactamase genotype in all 11 <em>Enterobacterales</em>. The AMR genetic islands in the aquatic bacteria were also found to share similar genetic structures similar to those of circulating ESBL bacteria causing human infections. These findings underscore the potential role of aquatic ESBL bacteria as AMR reservoirs for human pathogens, suggesting that aquatic bacteria may facilitate the hidden transmission of AMR mediated by MGEs through horizontal gene transfer across different sources and species, highlighting the importance of integrating environmental AMR monitoring into local surveillance strategies.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179353"},"PeriodicalIF":8.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rising occurrence of compound droughts and heatwaves in the Arabian Peninsula linked to large-scale atmospheric circulations","authors":"Md Saquib Saharwardi ,&nbsp;Waqar Ul Hassan ,&nbsp;Hari Prasad Dasari ,&nbsp;Harikishan Gandham ,&nbsp;Prajeesh Ag ,&nbsp;Ibrahim Hoteit","doi":"10.1016/j.scitotenv.2025.179433","DOIUrl":"10.1016/j.scitotenv.2025.179433","url":null,"abstract":"<div><div>Compound droughts and heatwaves (CDHWs) have emerged as a critical threat to global populations, with serious implications for water resources, agriculture, and ecosystems. Despite their significance, the occurrence and drivers of CDHW events remain inadequately understood, particularly in arid regions such as the Arabian Peninsula (AP). Using fifth-generation ECMWF reanalysis (ERA5) data, we analyze the spatiotemporal dynamics and variability of CDHW events over the AP from 1980 to 2023. Our results reveal a substantial increase in the frequency and intensity of CDHW events over this period, with a fourfold increase in the affected area since 1998. The northern parts of the AP are particularly vulnerable to CDHW events, due to the strong synchronization of heatwaves and droughts, derived by dominant role of temperature. We identify a robust linkage between CDHWs and large-scale circulation indices, notably the positive phase of Atlantic Multidecadal Oscillation (AMO) and the negative phase of Pacific Decadal Oscillation (PDO) have favored pronounced CDHWs over the past two decades. The AMO variability primary influences the mid-tropospheric pressure system leading to substantial temperature variations in the AP. However, the PDO largely impacts upper-level zonal winds, which modulate the strength of the subtropical westerly jet and subsequently changes the AP precipitation. Our findings highlight the urgent need for adaptive strategies and resilient measures to mitigate the adverse effects of CDHWs in a rapidly changing climate.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179433"},"PeriodicalIF":8.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and potential causes of a whiting event in the Mar Menor coastal lagoon (Mediterranean, SE Spain)","authors":"Marijn Oosterbaan ,&nbsp;Francisco Gómez-Jakobsen ,&nbsp;Gonzalo G. Barberá ,&nbsp;Jesús M. Mercado ,&nbsp;Isabel Ferrera ,&nbsp;Lidia Yebra ,&nbsp;Blas Valero-Garcés ,&nbsp;Antonio Delgado-Huertas ,&nbsp;Marta Álvarez ,&nbsp;Lázaro Marín-Guirao ,&nbsp;Pedro Martínez Martínez ,&nbsp;Victor Orenes-Salazar ,&nbsp;Marcel Galofré ,&nbsp;Arsenio Granados ,&nbsp;Cristóbal Verdugo ,&nbsp;Ana M. Cabello ,&nbsp;María Teresa Camarena-Gómez ,&nbsp;Carlota R. Gazulla ,&nbsp;Sophia Ouaissa ,&nbsp;Elena Pérez-Rubín ,&nbsp;Juan M. Ruíz","doi":"10.1016/j.scitotenv.2025.179391","DOIUrl":"10.1016/j.scitotenv.2025.179391","url":null,"abstract":"<div><div>The Mar Menor is a hypersaline coastal lagoon in the southeast of Spain, which has experienced several eutrophication episodes in recent years. In 2022, an area of milky white turbid water appeared covering about 10 % of its surface, and it has been there ever since. A comprehensive field research was set up to assess the origin of this possible whiting event, including identification of continental water inputs, satellite imagery and analyses of physico-chemical properties of the water column, sediment, and the planktonic and benthic communities. Our observations indicate that the water turned white due to precipitation of authigenic (formed where it is currently located) calcite as described in whiting events occurring in other marine areas. In addition to very high turbidity, the affected area showed increased nutrient and chlorophyll <em>a</em> concentrations compared to the rest of the lagoon. The altered conditions in this area also affected the planktonic communities and led to a complete disappearance of the previously abundant benthic vegetation (<em>Caulerpa prolifera</em>), thus influencing the biogeochemical cycles. Satellite images coupled with sediment core analysis suggest that this whiting event, which appears to have begun in 2022, is unprecedented in the Mar Menor. The enhanced alkalinity due to the semi-enclosed nature of the lagoon, along with the inflow of continental water may be producing favorable conditions for calcium carbonate supersaturation since rising groundwater levels in the adjacent aquifer seem to have intensified the groundwater discharge, which contains high concentrations of HCO₃^<em>−</em>. This rise is likely due to a long-term increase in recharge, induced by the expansion of irrigation practices, in combination with oscillations in rainfall cycles.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179391"},"PeriodicalIF":8.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal patterns and influences of monthly, seasonal and annual temperatures on methane emissions in Greece, Armenia and Russia over two decades","authors":"Abhishek Singh ,&nbsp;Anil Kumar Singh ,&nbsp;Sapna Rawat ,&nbsp;Vishnu D. Rajput ,&nbsp;Karen Ghazaryan ,&nbsp;Vicky Anand ,&nbsp;Tatiana Minkina ,&nbsp;Athanasios Alexiou ,&nbsp;Mohamed S. Elshikh ,&nbsp;João Ricardo Sousa ,&nbsp;Henrique Trindade ,&nbsp;Rupesh Kumar Singh ,&nbsp;Arunava Pradhan","doi":"10.1016/j.scitotenv.2025.179428","DOIUrl":"10.1016/j.scitotenv.2025.179428","url":null,"abstract":"<div><div>This study explores methane emission trends across Greece, Armenia, and Rostov Oblast region of Russia from 2004 to 2023. Our analyses, based on remote sensing and advanced statistical techniques, showed a 1.3–1.8 °C increase in mean annual temperature over this 20-year period in all these three regions, with the highest and the lowest rates of annual warming in Armenia (0.104 °C) and Rostov Oblast of Russia (0.052 °C), respectively. Mean annual methane concentrations increased distinctly in these regions over this period. Greece showed the trend of highest correlations between methane emissions and temperatures, including mean annual and seasonal temperatures, highlighting substantial role of climate change in emission trends. The emission trends with on-ground observations revealed intricate connections between reduced precipitations, farming practices, waste disposal methods, and naturally occurring emissions in Greece. In contrast, Armenia exhibited weak correlations between temperature and methane emissions, with its farming, waste management, energy and manufacturing sectors playing a significant role in determining emission quantities. The Rostov Oblast of Russia demonstrated weaker association between methane emissions and temperatures than Greece and Armenia, with emission trends being primarily shaped by agricultural activities and natural discharges from wetlands. The forecast models predicted further rise in methane emissions over the 7-year period (2024–2030), with the highest elevation rate estimated for Russia. This study emphasizes the need for tailored mitigation strategies to address methane emissions effectively, considering region-specific factors. Advanced monitoring technologies provide crucial insights into the assessment and management of methane emissions in these diverse geomorphological regions.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179428"},"PeriodicalIF":8.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish","authors":"Ulises G. Díaz-Avello ,&nbsp;Vasso Skouridou ,&nbsp;Xhensila Shkembi ,&nbsp;Jaume Reverté ,&nbsp;Manolis Mandalakis ,&nbsp;Panagiota Peristeraki ,&nbsp;Mònica Campàs ,&nbsp;Ciara K. O'Sullivan","doi":"10.1016/j.scitotenv.2025.179419","DOIUrl":"10.1016/j.scitotenv.2025.179419","url":null,"abstract":"<div><div>Tetrodotoxin (TTX) is a highly potent marine toxin which can cause severe poisoning following consumption of contaminated fish and seafood. Thus, a sensitive, reliable and simple test is required for rapid screening of samples and prevention of intoxication. Herein, we translated a previously reported microtiter plate hybrid aptamer-antibody assay into a rapid lateral flow assay (LFA) test. The test relies on an aptamer immobilized on the membrane and an antibody conjugated with gold nanoparticles to provide a visual result when TTX is present in the sample. The optimized test is simple (one-step), rapid (&lt;20 min), highly sensitive (visual limit of detection of 0.3 ng/mL TTX in buffer corresponding to 0.78 mg TTX/kg tissue), specific, reproducible and with long storage life. It was validated by analyzing contaminated pufferfish tissue extracts and it successfully detected TTX below the current limits set by official bodies. The analysis performed with this device in combination with a simple LFA reader for quantification was in excellent agreement with other established methods, further demonstrating the value of this test as a simple, low-cost and reliable analytical tool to ensure food safety, protect human health, and broaden the knowledge on the correlation between biological parameters and environmental data.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"978 ","pages":"Article 179419"},"PeriodicalIF":8.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}