Alejandro Godinez, Mohammed Alazawi, Milagros Neyra, Brianna Hanson, Dana Neigel, Kirsten St George, Dan Lang, David A Larsen
{"title":"Lessons learned from upstream wastewater sampling in response to poliovirus in New York State.","authors":"Alejandro Godinez, Mohammed Alazawi, Milagros Neyra, Brianna Hanson, Dana Neigel, Kirsten St George, Dan Lang, David A Larsen","doi":"10.1016/j.scitotenv.2025.180216","DOIUrl":"10.1016/j.scitotenv.2025.180216","url":null,"abstract":"<p><p>Emerging infectious diseases present a serious challenge for local health departments, and wastewater testing for pathogen surveillance is one approach to improve response preparedness. Sample collection is routinely conducted at wastewater treatment plants, and samples from wastewater treatment plants can conveniently be used for infectious disease surveillance. The sensitivity of wastewater surveillance for detecting emerging pathogens, however, is lower in high-population areas. In response to a paralytic polio case in Rockland County, the New York State wastewater surveillance network implemented wastewater surveillance in the immediate and neighboring counties, and additional wastewater surveillance at sampling points upstream from the wastewater treatment plants where positive samples were detected. The purpose of this paper is to describe the lessons learned from the rapid emergency deployment of upstream sampling in response to the poliovirus outbreak. Sampling upstream from the treatment plants at manholes in the system reduced the total sampled population in the primary treatment plant serving Rockland County from roughly 201,000 to sub-area populations ranging from roughly 3100 to 78,300, enabling more precise identification of where polio transmission was occurring. Detections across several of these upstream areas confirmed that poliovirus was not isolated to a single individual case or limited to a specific community. Maintaining upstream sampling was challenged by a lack of dedicated staff and funding. Implementation of upstream sampling highlighted the importance of preparation, including underlying data of the sewer infrastructure, coordination between government agencies, and readiness with sampling equipment. As the threat of emerging infectious diseases increases, due in part to increased vaccine hesitancy, well-planned upstream wastewater sampling strategies by health departments should be considered as additional outbreak response tools.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180216"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pham Duy Quang Dao, Seong Weon Lee, Ahsan Ali, Geondo Park, Sunghwan Kim, Chan Sik Cho, Ho-Jin Lim
{"title":"Oligomeric amide formation in secondary organic aerosol from the photooxidation of aromatics in the presence of ammonia.","authors":"Pham Duy Quang Dao, Seong Weon Lee, Ahsan Ali, Geondo Park, Sunghwan Kim, Chan Sik Cho, Ho-Jin Lim","doi":"10.1016/j.scitotenv.2025.180203","DOIUrl":"10.1016/j.scitotenv.2025.180203","url":null,"abstract":"<p><p>This study aims to enhance our understanding of the chemical composition and potential formation pathways of oligomeric amides in relation to the photooxidation of aromatic volatile organic compounds (VOCs) in atmospheric aerosol. Aromatic hydrocarbons (e.g., toluene, m-xylene, and ethylbenzene) undergo complex photooxidation processes that interact with NH<sub>3</sub>, leading to the formation of complex N-containing organic compounds. In this study, we successfully synthesized and characterized several oligomer amides, which were employed as standards to detect N-containing secondary organic aerosol (SOA) generated in a smog chamber under simulated atmospheric conditions. The results revealed the presence of oligomeric amides in the SOA, indicating their formation during the photooxidation process in the presence of NH<sub>3</sub> and providing significant insights related to the chemical formation pathway of SOA.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180203"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hojun Lee, Taeyeon Hong, Junho Park, Junhun Kweon, Shinhye Kim, Gwonhwa Song, Wooyoung Jeong, Whasun Lim
{"title":"Methomyl-induced developmental and cardiovascular toxicity in zebrafish via immune response, oxidative stress, and apoptosis.","authors":"Hojun Lee, Taeyeon Hong, Junho Park, Junhun Kweon, Shinhye Kim, Gwonhwa Song, Wooyoung Jeong, Whasun Lim","doi":"10.1016/j.scitotenv.2025.180215","DOIUrl":"10.1016/j.scitotenv.2025.180215","url":null,"abstract":"<p><p>Methomyl, a widely used carbamate pesticide, is frequently detected in aquatic ecosystems, due to its high water solubility and long environmental half-life. Although carbamate pesticides have been implicated in cardiovascular toxicity, the specific effects of methomyl on cardiovascular development remain largely unknown. In this study, we investigated the environmental and developmental effects of methomyl using Danio rerio (zebrafish) as an in vivo model. Methomyl exposure lowered cell viability and morphological abnormalities. Impaired cardiac development and disrupted vascular formation in zebrafish were confirmed using cmlc2:dsRed and fli1a:EGFP transgenic models. Furthermore, blood flow defects and erythrocyte accumulation were observed in gata1:dsRed transgenic model, suggesting compromised circulation. In addition, mpeg1:EGFP model revealed that methomyl activates the innate immune response in zebrafish. Molecular analyses revealed that methomyl exposure altered the expression of key genes involved in cardiac development, angiogenesis, and erythropoiesis, implicating oxidative stress and immune activation as potential underlying mechanisms. Human umbilical vein endothelial cells (HUVECs) were used as an in vitro model. We found that methomyl exhibited vascular toxicity in HUVECs, further supporting its role as a cardiovascular disruptor. These findings provide novel insights into the environmental and toxicological effects of methomyl and highlight its potential risk of accumulation in aquatic systems.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180215"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The ecology of cyanobacteria and their synergism with bacterioplankton in benthic mats under nutrient limitations in the Virgin River in Zion's National Park.","authors":"Abeer Sohrab, Shadman Kaiser, Bishav Bhattarai, Rosalina Stancheva, Ramesh Goel","doi":"10.1016/j.scitotenv.2025.180194","DOIUrl":"10.1016/j.scitotenv.2025.180194","url":null,"abstract":"<p><p>Nitrogen (N) and Phosphorus (P) are essential nutrients which support cyanobacterial growth in lakes (as suspended cells or flocs) and flowing waters (as benthic mats). However, some toxic cyanobacteria can thrive even under depleted nutrient conditions. In this study, we investigated benthic toxic cyanobacterial mats in the Virgin River, Zion National Park, at two sites where visible benthic mats were observed at three different times. Our study focused on phosphorus and nitrogen cycling within these mats. The reactive P was below detection limits (<0.05 mg/L as P). Likewise, the dissolved inorganic N varied from below the detection limit to 0.06 mg/L as N. The relative abundance (based on total sequenced reads) of cyanobacteria ranged from 16.87 % to 50.02 % for samples from the Visitor's Center site and 10.34 % to 32.68 % for samples from the North Creek site with the presence of genera Microcoleus, Pseudoanabaena, Oscillatoria, Microcystis, and Cyanobium. The Visitors Center and North Creek sites shared several key heterotrophic bacteria, with Sandarakinorhabdus, Planctomyces, Brevundimonas, Thiomonas, and Pseudomonas being the most prominent ones. Notably, a toxic Microcoleus strain was identified in the nutrient-limited conditions of the Virgin River. This strain harbored an anatoxin-a biosynthesis gene cluster but lacked the anaK gene, suggesting limited toxin variability. We investigated the role of heterotrophic bacteria in nutrient cycling and their interactions with cyanobacterial populations within benthic mats of the Virgin River. Microcoleus and other cyanobacteria employed the Pho regulon, expressing genes such as pstS and alkaline phosphatase, to efficiently acquire organic phosphorus in low-nutrient environments. Nitrogen cycling also played a crucial role, with coexisting heterotrophic bacteria contributing to nitrogen fixation (nif gene) and assimilation, as well as denitrification (nosZ, nir, and nar genes). This research signifies the importance of integrated molecular approaches in unraveling nutrient cycling dynamics and toxin production mechanisms in benthic cyanobacterial mats. These insights are critical for understanding harmful cyanobacterial benthic blooms in freshwater ecosystems.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180194"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diana Obregon, Daniela Hernandez Correal, Brian Eshenaur, Alejandro A Calixto
{"title":"Global use of the Environmental Impact Quotient: A tool to indicate potential pesticide risk.","authors":"Diana Obregon, Daniela Hernandez Correal, Brian Eshenaur, Alejandro A Calixto","doi":"10.1016/j.scitotenv.2025.180235","DOIUrl":"10.1016/j.scitotenv.2025.180235","url":null,"abstract":"<p><p>The Environmental Impact Quotient (EIQ) is a weighted, multi-component pesticide risk indicator (PRI) developed in 1992 by the New York State Integrated Pest Management Program (NYSIPM) at Cornell University. It serves as a decision-support tool to help growers, educators, and policymakers choose less hazardous pesticide options for farm workers, consumers, and non-target organisms. This systematic review presents data from 113 studies published between 1992 and 2024, along with Google Analytics metrics from the EIQ website, on the global use of the EIQ. Our findings indicate that the EIQ has been widely adopted, particularly in North America, and is commonly used to compare the application of Integrated Pest Management (IPM) strategies or the implementation of genetically modified crops. The EIQ is also used to track potential pesticide risk through time and across different localities. Corn, cotton, tomato, wheat, and soybeans are the crops with the highest number of studies implementing the EIQ. The EIQ has been compared to other PRIs and shows strong agreement with those incorporating similar environmental compartments. Many authors report selecting the EIQ over other PRIs due to its straightforward implementation across diverse systems. We also highlight the limitations of the EIQ, including the asymmetric weighting of its components, the limited scoring system, and the high correlation with the application rate. Recently, the NYSIPM introduced a wider scoring system to the EIQ based on institutional hazard classifications. While the EIQ remains a widely used tool, structural updates are needed to maintain its relevance and improve its performance.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180235"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melissa A H Turcotte, Heather E Jamieson, Michael B Parsons, Ryan Tappero, Derek R Knaack
{"title":"Arsenic fate in the historic Cobalt Mining Camp, Ontario: Geochemical, mineralogical, and vegetation-related controls on metal(loid) mobility.","authors":"Melissa A H Turcotte, Heather E Jamieson, Michael B Parsons, Ryan Tappero, Derek R Knaack","doi":"10.1016/j.scitotenv.2025.180171","DOIUrl":"10.1016/j.scitotenv.2025.180171","url":null,"abstract":"<p><p>This study investigates the controls on metal(loid) mobility from mine tailings and pond sediments near Cobalt, Ontario with a focus on arsenic (As), which is present at high concentrations throughout this former mining district. Mineralogical and geochemical analyses were performed on tailings from four abandoned mill sites and nearby pond sediments. The mass of readily soluble metal(loid)s in the tailings and sediments was evaluated using shake flask experiments. Horsetails (Equisetum spp.) growing on these tailings were collected for chemical characterization, backscattered electron imaging, and synchrotron-based chemical analysis. Results indicate that mineralogical hosts of As vary between depositional environments. Authigenic, oxidized phases (e.g., erythrite and an iron- and calcium-bearing arsenate) sequester As in near-surface environments. Ore minerals (e.g., cobaltite and safflorite) and authigenic reduced phases (e.g., realgar) host As in submerged tailings and anoxic pond sediments. Short-term exposure of tailings and sediments to oxidized, ultrapure water releases As from the ore minerals and reduced phases (1.31-325 mg L<sup>-1</sup> As; median = 19.1 mg L<sup>-1</sup> As). Horsetails growing on these tailings sequester As via formation of an oxidized iron-bearing plaque on the outside of the plant roots and shoots. This study demonstrates that As mobility in mine-impacted environments is controlled by geochemical reactions, mineral dissolution and precipitation, and vegetation growth. These processes must be considered when developing long-term management decisions for legacy mine sites.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180171"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Release of potentially harmful elements from excavated rocks with surface alteration under dry-wet cycles.","authors":"Yuto Yoshida, Hirofumi Sakanakura, Masahiko Katoh","doi":"10.1016/j.scitotenv.2025.180153","DOIUrl":"10.1016/j.scitotenv.2025.180153","url":null,"abstract":"<p><p>In construction projects, rocks containing potentially harmful elements (PHEs) may be excavated and treated for reuse. Thus, understanding the mechanisms of PHE release from excavated rocks that are to be reused is crucial. Embankments constructed using excavated rocks are primarily affected by exposure to dry-wet cycles. Herein, we aimed to 1) understand the causal relation between the surface alteration of excavated rocks caused by processes such as pyrite oxidation, fine granulation, and calcite and gypsum formation and changes in the PHE release from excavated rocks exposed to dry-wet cycles and 2) identify the changes causing variations in the amounts of different PHE species released from the involved excavated rocks. In the first month of the dry-wet cycles, pyrite oxidation, fine granulation, and gypsum formation were maximal. Progressive fine granulation with continuing dry-wet cycles led to further pyrite oxidation; however, no gypsum was formed after the first month. Fine granulation and gypsum formation did not change the amounts of PHE released. Arsenic, selenium, and cadmium were released from the excavated rocks as pyrite oxidation and fine granulation continued during dry-wet cycles. The amounts of arsenic and lead released during pyrite oxidation were minimized by iron-(hydr)oxides that precipitated, whereas the amounts of released selenium and cadmium, present in soluble forms, were not reduced. The continuous pyrite oxidation and fine granulation mainly controlled the phases of PHE and their release. Overall, the findings suggest that the changes in the amount of PHEs released due to surface alteration after the reuse of excavated rocks must be considered when evaluating their environmental risks.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180153"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In situ dosing of monochloramine in a hospital hot water system results in drastic microbial communities changes.","authors":"Marianne Grimard-Conea, Elliston Vallarino Reyes, Xavier Marchand-Senécal, Sébastien P Faucher, Michèle Prévost","doi":"10.1016/j.scitotenv.2025.180204","DOIUrl":"10.1016/j.scitotenv.2025.180204","url":null,"abstract":"<p><p>Understanding changes in microbial composition under selective pressures is crucial to assess the emergence of resistant taxa and the survival of drinking water-associated pathogens. This study evaluated the impact of in situ monochloramine disinfection in a hospital hot water system on bacterial (16S rRNA gene amplicon sequencing, 112 samples) and eukaryotic communities (18S rRNA gene amplicon sequencing, 103 samples), and on general microbial measurements (180 samples), including adenosine triphosphate (ATP) and flow cytometry counts. After the onset of treatment, ATP decreased by 1.2- and 3.5-fold, and total cell counts (TCC) dropped by 1- and 2-log at distal and system sites, respectively. During the dosage interruption (27-day), TCC rebounded to pre-treatment levels, but viability percentage decreased, indicating that cells were predominantly damaged. Low-use sites (e.g., showerheads) showed elevated ATP (>15 pg/mL) and TCC (10<sup>5</sup>-10<sup>6</sup> cells/L). Monochloramine drastically altered bacterial and eukaryotic communities. Alpha-diversity showed increased amplicon sequence variant richness during treatment, driven by new, low-abundant taxa, while Beta-diversity revealed distinct shifts in community composition over time, with tight or looser clusters corresponding to each treatment phase. Post-treatment, temporal and spatial heterogeneity was evident across distal sites, while elevated temperatures, consistent flow, and higher monochloramine concentrations in the hot water system resulted in more uniform communities at system sites. Additionally, the persistence of potential pathogenic strains belonging to Legionella and Mycobacterium genera highlights the value of comprehensive risk assessments. These findings emphasize the need to understand microbial shifts under disinfection stress and their public health implications, offering new insights into how treatment interventions shape microbial ecology and pathogen dynamics.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180204"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lennart V J van Melis, Arjuna R Persad, Anneloes M Peerdeman, Manon J H van Rossum, Regina G D M van Kleef, J Pepijn Wopken, Juliette Legler, Remco H S Westerink
{"title":"The effects of acute and chronic exposure to bisphenols and PFAS on neuronal activity and network development.","authors":"Lennart V J van Melis, Arjuna R Persad, Anneloes M Peerdeman, Manon J H van Rossum, Regina G D M van Kleef, J Pepijn Wopken, Juliette Legler, Remco H S Westerink","doi":"10.1016/j.scitotenv.2025.180214","DOIUrl":"10.1016/j.scitotenv.2025.180214","url":null,"abstract":"<p><p>Exposure to bisphenols and perfluoroalkyl substances (PFAS) is linked to various health impairments, including (developmental) neurotoxicity. Evidence indicates that bisphenols and PFAS can impact early neurodevelopmental processes such as proliferation, migration, and differentiation, although little is known about the effects of these compounds on neuronal activity and network development. Therefore, we assessed the effects of acute and chronic exposure to different bisphenols (bisphenol-A (BPA), bisphenol-F (BPF), and bisphenol-S (BPS)) and PFAS (perfluorooctanoate (PFOA), perfluorooctanesulfonate (PFOS), and perfluorohexanesulfonate (PFHxS)) on neuronal activity and network development in rat primary cortical cultures using micro-electrode array recordings. Acute exposure to BPA and BPF decreased neuronal activity, while BPS had no effect. Chronic exposure to 100 μM BPA decreased network development, while chronic exposure to 10 μM BPA, 100 μM BPF, and 100 μM BPS induced a hyperexcitation. Thus, differences in the molecular structure of bisphenols and exposure duration influence the effects of these compounds on neuronal activity and network development. In contrast, both acute and chronic exposure to PFOS, PFOA, and PFHxS had limited effects on neuronal activity and network development. Since bisphenols and PFAS are known endocrine-disrupting compounds, we also evaluated the possible involvement of estrogen, glucocorticoid, thyroid hormone, and aryl hydrocarbon receptor pathways in the observed neurotoxic effects. Our cortical cultures appeared insensitive to endocrine-mediated effects of (ant)agonists of these pathways, making it unlikely that the observed neurotoxic effects are endocrine-mediated. These findings contribute to hazard assessment for toxicological risk assessments and emphasize the need to consider molecular structure in evaluating neurotoxicity.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"997 ","pages":"180214"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hsien H. Khoo, Eugene H.Z. Ho, Ken S. Yap, Yang Zhao, Daren Z.L. Tan, Z. Yeo
{"title":"Life Cycle Assessment of biomass resources for hydrogen production","authors":"Hsien H. Khoo, Eugene H.Z. Ho, Ken S. Yap, Yang Zhao, Daren Z.L. Tan, Z. Yeo","doi":"10.1016/j.scitotenv.2025.180587","DOIUrl":"10.1016/j.scitotenv.2025.180587","url":null,"abstract":"<div><div>Energy demands are steadily increasing due to rising economy and industrial developments. There is a need to replace non-renewable fossil fuels with bio-based hydrogen. This article investigates various bio‑hydrogen production processes from 5 biomass resources: i) rice straw, ii) switchgrass, iii) sugarcane bagasse, iv) wheat straw, and v) corn stalk. Case studies of 12 bio‑hydrogen pathways were evaluated via LCA approach. The LCA impacts consist of Global Warming Potential (GWP), Acidification Potential (AP), Eutrophication Potential (EP), and Photochemical Ozone Creation Potential (POCP). The results demonstrated rice straw (RS) was the least favorable option utilized to produce 1 kg H<sub>2</sub> among all 12 options. Further comparisons were made concerning Energy demands, Water Footprint and Land Footprint. Normalization and Weighted scoring procedure were carried out. Among all 12 cases, the most favorable Normalized and Weighted impact scores were demonstrated by two cases (Impact scores of −7.61 and − 5.07) involving the gasification of switchgrass to produce hydrogen. A wide range of both carbon and water footprint results were displayed with the application of various bioprocesses for hydrogen generation. The wide range of results is due to the sets of varying environmental indicators applied for land and water use in LCA research areas. To further enhance the results, error analysis was also done for both energy requirements and water footprints.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1002 ","pages":"Article 180587"},"PeriodicalIF":8.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}