Science of the Total Environment最新文献

{"title":"Synergistic effects of heavy-metal-tolerant plant-growth-promoting bacteria on tall fescue growth and the phytoremediation of multi-metal-contaminated soils","authors":"Soo Yeon Lee,&nbsp;Minseo Yoon,&nbsp;Kyung-Suk Cho","doi":"10.1016/j.scitotenv.2026.181527","DOIUrl":"10.1016/j.scitotenv.2026.181527","url":null,"abstract":"<div><div>Heavy metal–contaminated soils pose serious threats to ecosystems, increasing the demand for sustainable and cost-effective remediation strategies. Although phytoremediation is an environmentally friendly approach, its efficiency is often limited under multi-metal stress, and the interactions among multiple plant growth–promoting bacteria (PGPB) remain insufficiently understood. In this study, we evaluated the effects of single and combined inoculation with three heavy metal–tolerant PGPB—<em>Acinetobacter</em> sp. ME1, <em>Kosakonia</em> sp. W18 and <em>Burkholderia</em> sp. ZF6—on the phytoremediation performance of tall fescue grown in soil co-contaminated with Cr, Zn, Cd and Pb. Dual inoculation with ME1 and W18 (D1 treatment) achieved over 30% removal efficiency for all four metals. Indole-3-acetic acid and dehydrogenase activities in the D1 and ME1 + ZF6 (D2) treatments were 1.1–1.3-fold higher than those in single-strain inoculations. The translocation factor in the D1 treatment was 1.7-fold higher than that of the uninoculated control, indicating enhanced metal uptake and translocation. Plant growth was also promoted, with root biomass exceeding 40 g. Microbial network and functional gene analyses revealed strengthened rhizosphere–endosphere connectivity and increased abundance of Cd/Zn tolerance genes under the D2 treatment. In contrast, triple-strain inoculation did not result in further improvements in metal removal, microbial functional indicators or plant growth compared with the dual-strain treatments. These results suggest that functional complementarity, rather than the number of inoculated strains, governs remediation efficiency. Overall, this study demonstrates that strategically designed multi-PGPB inoculation can enhance soil–plant–microbe interactions and improve phytoremediation performance in soils contaminated with multiple heavy metals.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181527"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological distribution and functional characterization of polyethylene-degrading enzymes from diverse metagenomes","authors":"Ning Wang ,&nbsp;Meiling Jin ,&nbsp;Zixuan Zhu ,&nbsp;Yuxin Wang ,&nbsp;Xinyu Li ,&nbsp;Jing Xu ,&nbsp;Shijunyi Cheng ,&nbsp;Yuanying Zhu ,&nbsp;Ruiqi Wang ,&nbsp;Tong Xu ,&nbsp;Furong Yin ,&nbsp;Xinyue Li ,&nbsp;Yue Ke ,&nbsp;Haitao Yue","doi":"10.1016/j.scitotenv.2026.181486","DOIUrl":"10.1016/j.scitotenv.2026.181486","url":null,"abstract":"<div><div>Polyethylene (PE), the most widely produced synthetic polymer, is highly resistant to degradation and poses long-term ecological risks due to its accumulation in terrestrial and aquatic ecosystems. Although biological degradation pathways have been investigated, research has primarily concentrated on heavily polluted environments, leaving the ecological distribution of PE-degrading enzymes largely unknown. In this study, we systematically screened more than 4.57 billion metagenomic sequences from diverse ecological sources—including farmland soils, Przewalski's horse gut microbiota, insect symbionts, and human oral microbiomes—for homologs of known PE-degrading enzymes. A total of 701 candidate sequences were identified using an integrated pipeline combining sequence homology, structural modeling, and molecular docking. Thirty-two representative enzymes were heterologously expressed and tested on pristine PE films and microspheres, among which 25 exhibited measurable activity, inducing surface erosion, up to ∼1.5% mass loss (<em>w</em>/w) of PE films over 30 days, and oxidative modifications. These degradative effects were validated by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), and stable carbon isotope (δ¹³C) analysis, collectively supporting molecular-level oxidation and early-stage carbon turnover associated with enzymatic PE degradation. Notably, PE-degrading activity was observed in microbiomes from relatively minimally disturbed environments, suggesting that microbial communities may adaptively evolve plastic-degrading capabilities in response to chronic, low-level exposure. These findings expand our understanding of plastic pollution's ecological footprint and highlight naturally occurring enzymes as promising candidates for sustainable bioremediation.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181486"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving bioleaching of Cu and Zn from e-waste with graphene and activated carbon with Acidithiobacillus bacteria","authors":"Hamideh Badri ,&nbsp;Mahdokht Arshadi ,&nbsp;Fatemeh Pourhossein ,&nbsp;Mahya Kheirandish ,&nbsp;Soheila Yaghmaei","doi":"10.1016/j.scitotenv.2026.181357","DOIUrl":"10.1016/j.scitotenv.2026.181357","url":null,"abstract":"<div><div>The rapid growth of e-waste generation poses significant environmental hazards and represents a potential source of valuable metals. This study investigates the bioleaching of copper (Cu) and zinc (Zn) from television printed boards (TVPCBs) using <em>Acidithiobacillus ferrooxidans (A. ferrooxidans)</em> and <em>Acidithiobacillus thiooxidans (A. thiooxidans)</em>, both separately and mixture of them. The use of graphene and powdered activated carbon as catalysts was investigated to enhance metal recovery. Response surface methodology (RSM) with a central composite design (CCD) was applied to optimize critical variables, including initial pH, graphene concentration, and activated carbon concentration. The mixed bacterial culture improved Cu recovery, reaching values 18 % higher than those obtained with <em>A. ferrooxidans</em> alone and 15 % higher than with <em>A. thiooxidans</em> alone. Cu recovery increased by approximately 20 % when using graphene and powdered activated carbon as catalysts. Under optimal conditions (initial pH 2.0, 1.5 g/L graphene, and 1.5 g/L activated carbon), 100 % simultaneous recovery of Cu and Zn was achieved within 12 days. Catalysts greatly enhanced bacterial activity, which was evidenced by the increased metal recovery. This effect may be attributed to the increased concentrations of sulfate and ferric ions.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181357"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-mediated niche differentiation promotes comammox-dominated nitrification in aquaponic rhizospheres","authors":"Mingchen Xu ,&nbsp;Liang Chen ,&nbsp;Hua Zou","doi":"10.1016/j.scitotenv.2026.181517","DOIUrl":"10.1016/j.scitotenv.2026.181517","url":null,"abstract":"<div><div>Sustainable aquaculture requires efficient nitrogen management and reduced N₂O emissions. Integrating plants into these systems (aquaponics) represents a promising strategy to achieve these goals. In this study, a tilapia–chive aquaponics model was used to investigated the mechanisms by which plant root exudates regulate rhizosphere nitrifier microbial communities, with a focus on complete ammonia oxidizers (comammox). Over 70 days, we probed how aquaponics and hydroponic conditions reshape the rhizosphere microbiome using qPCR, qFISH, and selective inhibitor assays to quantify the functional contributions of comammox, AOA, and AOB to nitrification and N₂O production. Results showed that the aquaponics system enhanced root exudation, increasing rhizosphere total organic carbon and creating a mildly acidic micro-environment that selectively enriched comammox. Comammox ultimately dominated the ammonia-oxidizing community (99% in abundance) and activity (98% of nitrification capacity) by the end of the experiment. Consequently, the aquaponics system achieved a sustained reduction in the N₂O conversion rate compared to the hydroponic controls, exceeding 50% in all sampling stages after day 7. This mitigation is attributed to minimized nitrite (NO₂⁻-N) accumulation and the inherently low N₂O-yielding potential of comammox. These findings reveal a plant-driven mechanism that selects for comammox-dominated nitrification, effectively curbing N₂O emissions while maintaining efficient N conversion. The results offer a potential strategy for improving nitrogen use efficiency and emission control in integrated aquaculture systems.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181517"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wastewater-based surveillance as a tool for monitoring and estimating COVID-19 incidence and trends: Insights from Germany, 2022–2024","authors":"Susan Abunijela ,&nbsp;Peter Pütz ,&nbsp;Timo Greiner,&nbsp;Ann-Sophie Lehfeld,&nbsp;Alexander Schattschneider,&nbsp;Udo Buchholz ,&nbsp;Jakob Schumacher","doi":"10.1016/j.scitotenv.2025.181290","DOIUrl":"10.1016/j.scitotenv.2025.181290","url":null,"abstract":"<div><h3>Background</h3><div>Wastewater-based surveillance complements case-based surveillance systems by capturing pathogen signals shed in stool and other bodily excretions, enabling population-level monitoring independent of clinical testing. Its utility during the COVID-19 pandemic has been widely explored, but its responsiveness and interpretability relative to case-based systems remain insufficiently understood.</div></div><div><h3>Methods</h3><div>We analyzed German nationwide data on COVID-19 or SARS-CoV-2 from July 2022 to December 2024, using wastewater surveillance and four case-based surveillance systems. These comprise syndromic surveillance systems at the population as well as the primary care level, and mainly laboratory-confirmed notification data, all aimed at monitoring COVID-19 incidence in Germany. We assessed agreement between wastewater viral load and disease incidence using visual inspection, cross-correlation analysis, and an estimated prevalence dynamic informed by a fecal shedding model. We derived retrospective translation factors and compared week-to-week trend directions between systems. Finally, we tested the predictive power of wastewater data using classification models to anticipate current week incidence trends.</div></div><div><h3>Results</h3><div>Wastewater SARS-CoV-2 viral load closely correlates with COVID-19 incidence trends from case-based systems, showing similar timing of peaks and troughs without notable time lags. Cross-correlation coefficients are highest with syndromic surveillance systems (up to 0.87) and lowest with notification data (0.43). Retrospective translation into incidence estimates works well on average, but week-to-week translation varies considerably. Wastewater-based models correctly predict the current week’s trend, as indicated by at least three of the four case-based systems, with about 68 % probability.</div></div><div><h3>Conclusion</h3><div>Wastewater surveillance correlates well with COVID-19 incidence, but real-time translation to incidence lacks precision. Trend prediction for the current week may demonstrate improved accuracy and may be valuable when case reporting is limited or delayed.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181290"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “The chemical evolution of tributaries to Lake George (Essex County), New York (USA), 1970–2024: Recovery from acidic atmospheric deposition and the impact of road salt” [Sci. Total Environ., 1015 (2026), 181402]","authors":"J.W. Sutherland ,&nbsp;S.A. Norton ,&nbsp;B.J. Cosby ,&nbsp;C. Navitsky","doi":"10.1016/j.scitotenv.2026.181575","DOIUrl":"10.1016/j.scitotenv.2026.181575","url":null,"abstract":"","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181575"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Mechanical upcycling of single-use face mask waste into high-performance composites: An ecofriendly approach with cost-benefit analysis” [Sci. Total Environ., 919 (2024), 170469]","authors":"Omid Zabihi ,&nbsp;Rebecca Patrick ,&nbsp;Mojtaba Ahmadi ,&nbsp;Mike Forrester ,&nbsp;Rachel Huxley ,&nbsp;Minoo Naebe","doi":"10.1016/j.scitotenv.2026.181557","DOIUrl":"10.1016/j.scitotenv.2026.181557","url":null,"abstract":"","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181557"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and diversity of human enteric viruses in bivalve mollusks from the Amazon mangrove ecosystem in Brazil","authors":"Isabella Rodrigues Negreiros ,&nbsp;Maria Luisa Rabelo Evas ,&nbsp;Guilherme Caetano Lanzieri Tavares ,&nbsp;Natália Lourenço dos Santos ,&nbsp;Adriana Gonçalves Maranhão ,&nbsp;·. Bruna Barbosa de Paula ,&nbsp;Flávia Freitas de Oliveira Bonfim ,&nbsp;Livia Melo Villar ,&nbsp;Tulio Machado Fumian ,&nbsp;Nancyleni Pinto Chaves Bezerra ,&nbsp;Alcina Vieira de Carvalho-Neta ,&nbsp;José Paulo Gagliardi Leite ,&nbsp;Marize Pereira Miagostovich ,&nbsp;Carina Pacheco Cantelli","doi":"10.1016/j.scitotenv.2026.181489","DOIUrl":"10.1016/j.scitotenv.2026.181489","url":null,"abstract":"<div><div>Coastal mangroves are vital ecosystems located at the land-sea interface and, thus, highly vulnerable to anthropogenic contamination. Among their rich biodiversity, these areas host bivalve mollusks recognized as waterborne pathogen bioindicators. In this sense, this study aimed to assess the occurrence and genetic diversity of multiple human enteric viruses and the presence of the human fecal bioindicator crAssphage in bivalve mollusks from the Amazon Mangrove Region in Brazil, a shellfish harvesting and consumption hotspot. Oysters and mussels were sampled from three Maranhão Island municipalities, in the state of Maranhão, Northeastern Brazil, between August 2023 and July 2024. Eighty-nine samples were processed according to the ISO 15216-1:2017 standard. Viral screening was performed by the TaqMan real-time PCR assay, and molecular characterization by Sanger sequencing. Rotavirus group A and norovirus GII were the most prevalent enteric viruses (18%, each), followed by human mastadenovirus (14.6%), norovirus GI (6.7%), and hepatitis A virus (3.4%), while RVA showed the highest viral load (2.6 × 10⁶ gc/g). Detected genotypes included norovirus GII.8 and GI.5, RVA G3, P[8]-III, and I2, HAdV-F41, and HAV IA-III. Viral occurrence was higher during the rainy season, coinciding with increased runoff and sewage inputs. CrAssphage exhibited the highest detection frequency (38.2%) and showed significant positive associations with multiple enteric viruses. These findings represent the first report of multiple enteric viruses in bivalves from natural Amazon mangrove banks in Maranhão, highlighting a recognized food safety hazard associated with shellfish consumption.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181489"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “The effect of fire on the carbon fluxes and productivity of Brazilian woodland savannas” [Science of The Total Environment volume 987 (2025) 179626]","authors":"Francisco Navarro-Rosales ,&nbsp;Maria Antonia Carniello ,&nbsp;Wesley Jonatar Alves da Cruz ,&nbsp;Flavio de Campos Oliveira ,&nbsp;Huanyuan Zhang-Zheng ,&nbsp;Valéria Lucélia de Oliveira Corrêa ,&nbsp;Marcelo Leandro Feitosa de Andrade ,&nbsp;Yadvinder Malhi ,&nbsp;Andrew Hector ,&nbsp;Imma Oliveras Menor","doi":"10.1016/j.scitotenv.2026.181538","DOIUrl":"10.1016/j.scitotenv.2026.181538","url":null,"abstract":"","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181538"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attributing PM sources within a swine production facility via PMF modeling of particle size distribution data","authors":"Pradeep Kumar ,&nbsp;Seyit Uguz ,&nbsp;Shalini Tiwari ,&nbsp;Youwen Yang ,&nbsp;Zonggang Li ,&nbsp;Yuanhui Zhang ,&nbsp;Xufei Yang","doi":"10.1016/j.scitotenv.2026.181519","DOIUrl":"10.1016/j.scitotenv.2026.181519","url":null,"abstract":"<div><div>Particulate matter (PM) in swine barns poses a significant health risk to both animals and caretakers, yet the contributions of individual sources remain poorly understood. This study measured PM concentrations, as well as particle size distributions (PSDs), in a wean-to-finish production room and a farm office using a Grimm 11-D optical particle counter (OPC) during both summer and winter. Source attribution was conducted through PMF modeling of obtained PSD data. PM concentrations, including PM_2.5, PM₁₀, and total suspended particles (TSP), were higher in the production room during winter and lower in the office during summer. In all scenarios, PMF modeling effectively identified the potential sources of PM and quantified their relative contributions. In the production room during winter, the primary contributors to TSP were animal feed (41.6%), settled dust (29.7%), and feces (15.0%). Although PM introduced through ventilation and infiltration was a minor contributor to overall TSP, it accounted for a substantial portion of submicron particles. Additionally, an Alphasense OPC-N3 sensor was evaluated as a low-cost alternative to the Grimm 11-D for PM source attribution and yielded promising results. This study supports the use of PSD-based PMF modeling as a cost-effective approach for PM source attribution in animal production environments and is anticipated to facilitate animal agriculture air quality management.</div></div>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":"1018 ","pages":"Article 181519"},"PeriodicalIF":8.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}