Nano Today最新文献_第6页

Supramolecular photothermal agents mediated by black hole hosts 由黑洞宿主介导的超分子光热剂

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-10-05 DOI: 10.1016/j.nantod.2024.102520

Jie Gao , Shangli Ding , Ling Chen , Hongyu Li , Wen-Chao Geng , Dong-Sheng Guo , Zeli Yuan

引用次数: 0

Optically active persistent luminescence in supramolecular nanoassemblies constructed from entirely achiral building blocks 完全非手性结构单元构建的超分子纳米组装体中的光学活性持久荧光

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-10-03 DOI: 10.1016/j.nantod.2024.102516

Haolai Mao , Xue Jin , Chengxi Li , Chengyu Jiang , Xuefeng Yang , Zhijie Wang , Da-Wei Fu , Yi Zhang , Pengfei Duan

{"title":"Optically active persistent luminescence in supramolecular nanoassemblies constructed from entirely achiral building blocks","authors":"Haolai Mao , Xue Jin , Chengxi Li , Chengyu Jiang , Xuefeng Yang , Zhijie Wang , Da-Wei Fu , Yi Zhang , Pengfei Duan","doi":"10.1016/j.nantod.2024.102516","DOIUrl":"10.1016/j.nantod.2024.102516","url":null,"abstract":"<div><div>Optically active persistent luminescent materials have attracted significant attention due to their distinctive luminescent characteristics and ability to exhibit rich circular polarization information. Despite extensive efforts to develop circularly polarized persistent luminescence (CPPL) materials using chiral molecules or polymers, fabricating CPPL materials from achiral units remains a big challenge. In this work, we introduce an efficient co-assembly strategy to create CPPL materials using entirely achiral organic molecules. The optical activities of co-assembled complexes are attributed to structural chirality, which arises from chiral nanohelices formed during symmetry breaking in the self-assembly process of <em>C</em><sub>3</sub>-symmetric molecules. Achiral molecules with long-lasting phosphorescence can adhere to these chiral nanostructures via hydrogen bonding, and during the drying phase, form nanocrystals that align along helical fibers, resulting in circularly polarized, long-lasting phosphorescence. Enhanced CPPL efficiency, ranging from blue to yellow with a dissymmetry factor over 1.2 × 10<sup>−2</sup> and lifetime of up to 0.6 s at room temperature, is achieved through hydrogen bonding driven co-assembly. Additionally, the CPPL spectra of these co-assemblies are captured using a homemade time-resolved circularly polarized long afterglow detection platform. This study not only presents a new approach for the high-efficiency design of CPPL materials from achiral building blocks but also significantly broadens the research possibilities in real-time CPPL analysis, offering a horizon in the exploration of CPPL materials.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102516"},"PeriodicalIF":13.2,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ engineering of mRNA-CAR T cells using spleen-targeted ionizable lipid nanoparticles to eliminate cancer cells 利用脾脏靶向可离子化脂质纳米颗粒原位工程设计 mRNA-CAR T 细胞，以消灭癌细胞

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-10-03 DOI: 10.1016/j.nantod.2024.102518

Yuan Li , Yishi Tian , Chanjuan Li , Wenli Fang , Xiaohong Li , Zhangyan Jing , Zhaoxin Yang , Xiaozhou Zhang , Yanlan Huang , Jiaqi Gong , Fanqiang Meng , Lin Qi , Xin Liang , Linlin Hou , Kai Lv , Xudong Zhang

{"title":"In situ engineering of mRNA-CAR T cells using spleen-targeted ionizable lipid nanoparticles to eliminate cancer cells","authors":"Yuan Li , Yishi Tian , Chanjuan Li , Wenli Fang , Xiaohong Li , Zhangyan Jing , Zhaoxin Yang , Xiaozhou Zhang , Yanlan Huang , Jiaqi Gong , Fanqiang Meng , Lin Qi , Xin Liang , Linlin Hou , Kai Lv , Xudong Zhang","doi":"10.1016/j.nantod.2024.102518","DOIUrl":"10.1016/j.nantod.2024.102518","url":null,"abstract":"<div><div>Chimeric antigen receptor (CAR) T cell therapy has implemented impressive advances in the treatment of B-cell lymphoma. However, the complex production process of CAR T cells and hindrance of solid tumor penetration remain substantial challenges. Intriguingly, cell-targeting delivery of messenger RNA (mRNA) with ionizable lipid nanoparticles (mRNA-LNPs) is able to efficiently and precisely engineer T cells and other immune cells <em>in vivo</em> to perform their functions. Herein, we harnessed the ionizable LNPs to encapsulate mRNA encoding anti-tyrosinase related protein 1 (TRP1) CAR (CAR-LNPs) for <em>in vivo</em> generation of mRNA-CAR T cells to eliminate melanoma cells. Specifically, the anti-CD3 antibody (aCD3) armed mRNA-LNPs (CD3-mRNA-LNPs) selectively targeted T cells, resulting in the production of functional and therapeutic levels of CAR T cells both <em>ex vivo</em> and <em>in vivo</em>. These CD3-CAR-LNPs engineered CAR T cells were capable of infiltrating into the solid tumor and effectively eliminating melanoma cells with high TRP1 expression, significantly hindering tumor progression. Critically, CD3–7CAR-LNPs containing mRNA encoding both CAR and interleukin-7 (IL-7) generated 7CAR T cells that secreted IL-7, thereby enhancing the activity and proliferation of both CAR T cells and other intratumoral cytotoxic T cells. Alternatively, the employment of anti-programmed cell death protein 1 antibody (aPD-1) protected mRNA-CAR T cells from exhaustion, especially in combination with CD3–7CAR-LNPs, could significantly enhance the antitumor capability of CAR T cells without causing acute cytokine release syndrome (CRS).</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102518"},"PeriodicalIF":13.2,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long in vivo circulating nanomicelles formed by sharp-contrast Janus star polymers derived from β-cyclodextrin grafted with lipids and polyzwitterions 由与脂质和聚齐聚糖接枝的β-环糊精衍生的尖锐对比 Janus 星聚合物形成的体内循环纳米长小孔

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-10-02 DOI: 10.1016/j.nantod.2024.102511

Miao Zhang , Yuting Wen , Zhongxing Zhang , Xia Song , Jingling Zhu , Chitinart Thedrattanawong , Hongzhen Bai , Guping Tang , Kazushi Ogata , Shin-ichi Yusa , Jun Li

{"title":"Long in vivo circulating nanomicelles formed by sharp-contrast Janus star polymers derived from β-cyclodextrin grafted with lipids and polyzwitterions","authors":"Miao Zhang , Yuting Wen , Zhongxing Zhang , Xia Song , Jingling Zhu , Chitinart Thedrattanawong , Hongzhen Bai , Guping Tang , Kazushi Ogata , Shin-ichi Yusa , Jun Li","doi":"10.1016/j.nantod.2024.102511","DOIUrl":"10.1016/j.nantod.2024.102511","url":null,"abstract":"<div><div>Amphiphilic block polymers have the ability to self-assemble and form nanomicelles, which have been extensively studied as nanocarriers for improving the bioavailability and biodistribution of chemotherapeutic drugs while reducing systemic toxicity. However, polymer micelles often face issues with poor stability in the bloodstream, leading to a short circulation time and leakage of the payload. Here, this work reports a rational design of a sharp-contrast Janus star polymer (SJSP) consisting of multiple arms of superhydrophobic lipid moieties and superhydrophilic polyzwitterion chains attached to a β-cyclodextrin core. The SJSP polymer forms nanomicelles possessing a stable core and a controllable and dense stealth shell that effectively protects them in the bloodstream, preventing payload leakage and blood protein adsorption. It is demonstrated that the hydrophobic/hydrophilic balance can be optimized to achieve strong micellar assembly by adjusting the number of lipid and polyzwitterion arms. The SJSP micelle system shows significantly longer blood circulation time <em>in vivo</em> compared to linear counterparts and other available amphiphilic block copolymer micelle systems. Therefore, the SJSP micelle design offers a promising strategy for developing nanocarriers with potential for translational applications <em>in vivo</em>.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102511"},"PeriodicalIF":13.2,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ternary composite fluorescent films with tunable color and long lifetime based on efficient TS-FRET 基于高效 TS-FRET 的颜色可调、寿命长的三元复合荧光膜

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-10-01 DOI: 10.1016/j.nantod.2024.102515

Li Gao , Zhuoyao Shen , Wensheng Xu , Jiang Liu , Qinglong Jia , Chenyang Jin , Ligong Chen , Bowei Wang

{"title":"Ternary composite fluorescent films with tunable color and long lifetime based on efficient TS-FRET","authors":"Li Gao , Zhuoyao Shen , Wensheng Xu , Jiang Liu , Qinglong Jia , Chenyang Jin , Ligong Chen , Bowei Wang","doi":"10.1016/j.nantod.2024.102515","DOIUrl":"10.1016/j.nantod.2024.102515","url":null,"abstract":"<div><div>Multi-color long-wavelength organic afterglow materials are of great significance in anti-counterfeiting, but their preparation is still challenging. In this paper, a series of room temperature phosphorescence (RTP) films were constructed with polyvinyl alcohol (PVA) as rigid matrix and 9,10-diaminophenanthrene (DAphe) as guest molecule. Surprisingly, by adjusting the doping content of DAphe, their RTP emission peak width could be adjusted accordingly, and the lifetime was up to 3.25 s. Their dopant content dependent and excitation wavelength dependent luminescence characteristics and theoretical calculation results indicated that the observed broad emission peaks of RTP might be attributed to multiple luminescence centers generated by the aggregation of guest molecules. Interestingly, by doping several suitable fluorescent dyes screened as energy acceptors, multi-color, long-lasting afterglow composite films from blue to red were obtained, achieving 0.42 s delayed fluorescence at 661 nm with a fluorescence quantum yield of 32.22 %. In addition, these adjustable afterglow materials had good molding processability, so several cryptographic patterns were achieved to demonstrate their good application prospects in advanced anti-counterfeiting technologies.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102515"},"PeriodicalIF":13.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional dendrimer-peptide conjugates for MET receptor-specific imaging of cancer cells 用于癌细胞 MET 受体特异性成像的多功能树枝状聚合物-多肽共轭物

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-10-01 DOI: 10.1016/j.nantod.2024.102509

Jin Woong Lee , Kwangok P. Nickel , Rachel L. Minne , Justin J. Jeffery , Eduardo Aluicio-Sarduy , Carter Kim , DaWon Kim , Piper A. Rawding , Michael J. Poellmann , Narsimha Mamidi , Jonathan W. Engle , Jung Heon Lee , Hansoo Park , Reinier Hernandez , Randall J. Kimple , Andrew M. Baschnagel , Seungpyo Hong

{"title":"Multifunctional dendrimer-peptide conjugates for MET receptor-specific imaging of cancer cells","authors":"Jin Woong Lee , Kwangok P. Nickel , Rachel L. Minne , Justin J. Jeffery , Eduardo Aluicio-Sarduy , Carter Kim , DaWon Kim , Piper A. Rawding , Michael J. Poellmann , Narsimha Mamidi , Jonathan W. Engle , Jung Heon Lee , Hansoo Park , Reinier Hernandez , Randall J. Kimple , Andrew M. Baschnagel , Seungpyo Hong","doi":"10.1016/j.nantod.2024.102509","DOIUrl":"10.1016/j.nantod.2024.102509","url":null,"abstract":"<div><div>The Mesenchymal Epithelial Transition (MET) receptor tyrosine kinase is frequently upregulated or mutated in various cancers. Targeting MET signaling pathway has been utilized as a treatment for cancer, since MET overexpression is often associated with poor prognosis. Selective imaging of MET-overexpressing tumor cells would thus provide a high diagnostic value; however, it remains elusive due to a lack of targeted imaging contrast agents. Herein, we have developed a multifunctional diagnostic dendrimer-peptide conjugate (DPC) system with a strong avidity to MET-expressing cancer cells. The system was prepared by conjugating MET-inhibiting peptides (C7) to generation 7 (G7) poly(amidoamine) (PAMAM) dendrimers. Due to the dendrimer-mediated multivalent binding effect, the DPCs exhibited a significantly stronger binding to the human MET protein than free C7, as measured using surface plasmon resonance. Confocal microscopy revealed increased binding of the DPCs to the MET-expressing EBC-1 and UW-Lung-21 cells, whereas a MET knock-out cell line showed negligible interactions with the DPCs. The DPCs were then conjugated with Zirconium-89 for positron emission tomography and computed tomography (PET/CT) scanning, demonstrating their selective accumulation to MET-expressing tumors <em>in vivo</em>. Additionally, the plasma half-life of the DPCs was measured at ∼53 hours, which was significantly longer than free C7. These results collectively suggest that this DPC system has potential as a targeted imaging platform specific to MET-expressing tumors, which would be applicable to various cancer types.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102509"},"PeriodicalIF":13.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complement opsonized protein corona activated by precoated immunoglobulin enables neutrophil-hitchhiking for rapid and enhanced drug delivery for acute liver failure recovery 由预包被免疫球蛋白激活的补体溶解蛋白电晕可使中性粒细胞搭便车，从而快速增强药物输送，促进急性肝功能衰竭的恢复

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-09-28 DOI: 10.1016/j.nantod.2024.102512

Keying Chen , Chunxiong Zheng , Yunjuan Lv , Pengkai Zhao , Tong Lin , Yanteng Xu , Huimin Kong , Ke Yi , Qingguo Zhong , Mingqiang Li , Yu Tao , Haixia Wang

{"title":"Complement opsonized protein corona activated by precoated immunoglobulin enables neutrophil-hitchhiking for rapid and enhanced drug delivery for acute liver failure recovery","authors":"Keying Chen , Chunxiong Zheng , Yunjuan Lv , Pengkai Zhao , Tong Lin , Yanteng Xu , Huimin Kong , Ke Yi , Qingguo Zhong , Mingqiang Li , Yu Tao , Haixia Wang","doi":"10.1016/j.nantod.2024.102512","DOIUrl":"10.1016/j.nantod.2024.102512","url":null,"abstract":"<div><div>In acute inflammation, the heightened activation and recruitment of immune cells present an opportunity to leverage them as natural carriers for efficient transport of diagnostic probes and nanotherapeutics. Although complement opsonization facilitates the internalization of nanomedicines by activated neutrophils, the development of strategies to specifically augment complement deposition remains a challenge. Herein, we engineer silymarin-loaded liposomes (Lips) coated with immunoglobulin G (IgG) to enhance complement 3 (C3) deposition in the protein corona, thereby enabling neutrophil-mediated, precise targeting to the site of inflammation. Through the examination of various serum proteins, we discover that IgG adsorption, particularly with its Fc portion exposed, prominently promotes C3 enrichment in the protein corona, resulting in C3 cleavage into iC3b. This facilitates the uptake of C3-enriched Lips by activated neutrophils with elevated C3 receptor expression, thus improving the efficiency and specificity of nanomedicine delivery to inflammatory site. Following the formation of neutrophil extracellular traps, the released nanomedicine effectively mitigates hepatocyte damage by eliminating accumulated reactive oxygen species and inducing a shift in macrophage polarization towards the anti-inflammatory M2 phenotype. Our IgG-modified nanomedicine demonstrates significant therapeutic efficacy against acute liver failure by regulating the protein corona and hitchhiking neutrophils, offering a promising strategy for efficient and precise treatment of inflammation.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102512"},"PeriodicalIF":13.2,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-cascade ROS-trapping bioreaction system reverses stem cell oxidative stress fate for osteogenesis 自级联 ROS 捕获生物反应系统逆转干细胞氧化应激命运，促进骨生成

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-09-28 DOI: 10.1016/j.nantod.2024.102514

Jiawei Yang , Hao Gu , Yuhui Zhu , Jiaojiao Shao , Haishuang Chang , Mingliang Zhou , Jie Wang , Xinquan Jiang

{"title":"Self-cascade ROS-trapping bioreaction system reverses stem cell oxidative stress fate for osteogenesis","authors":"Jiawei Yang , Hao Gu , Yuhui Zhu , Jiaojiao Shao , Haishuang Chang , Mingliang Zhou , Jie Wang , Xinquan Jiang","doi":"10.1016/j.nantod.2024.102514","DOIUrl":"10.1016/j.nantod.2024.102514","url":null,"abstract":"<div><div>Reactive oxygen species (ROS) scavenging is essential for periodontal regeneration. However, the dynamic change of the applied materials within the ROS-rich environment and the residual oxidation products in the host highly impact periodontal regeneration. This study successfully constructs a bioreaction system via thiol-ene click chemistry, leveraging the high affinity of glutathione (GSH) for ROS to attract excess ROS to the crosslinking points. Two minutes after hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment, the ROS level in the G8–0 hydrogel acutely decreases, reaching a 4.4 % reduction within 10 minutes, confirming the ROS-trapping efficacy. Through a ‘bait switch-on’ mechanism, hexagonal boron nitride (hBN) takes over the captured ROS and the oxidation products of pectin further drive the reduction reaction, ultimately restoring the extracellular environment. The self-cascade products, oxidized hBN, reshape the intracellular oxidative stress (OS) environment, achieving a synergistic extra- and intra-cellular treatment. The significantly high reduced to oxidized glutathione (GSH/GSSG) ratio in G8–10 hydrogel (∼80 %) illustrates a reversal of oxidative stress in bone marrow stem cells (BMSCs). On a molecular level, the bioreaction system inhibits the NF-κB pathway, promoting the expression of key antioxidant genes (<em>Nqo1</em> and <em>Nrf2</em>) and osteogenic molecules (ALP and OCN), thereby reversing the detrimental effects of OS on BMSCs. In vivo application demonstrated the system’s strong redox-balancing and osteogenic capabilities in the periodontal inflammation environment. This novel antioxidant bioreaction system, characterized by self-cascade ROS-trapping and product utilization, offers innovative treatment strategies for tissue regeneration under conditions of excessive OS.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102514"},"PeriodicalIF":13.2,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA nanotube-carrying antimicrobial peptide confers improved anti-infective therapy 携带抗菌肽的 DNA 纳米管可提高抗感染治疗效果

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-09-27 DOI: 10.1016/j.nantod.2024.102508

Nier Wu , Shengnan Fu , Chenxi Dai , Lingfei Hu , Jiaxin Li , Fangzhou Chen , Lin Wang , Xin Su , Dongsheng Zhou

{"title":"DNA nanotube-carrying antimicrobial peptide confers improved anti-infective therapy","authors":"Nier Wu , Shengnan Fu , Chenxi Dai , Lingfei Hu , Jiaxin Li , Fangzhou Chen , Lin Wang , Xin Su , Dongsheng Zhou","doi":"10.1016/j.nantod.2024.102508","DOIUrl":"10.1016/j.nantod.2024.102508","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) represent a good alternative for treating infections to overcome increasing antibiotic resistance problems. DNA nanostructures have been utilized as the delivery carrier of AMPs to enhance their performance, but mechanisms of action remain largely unclear. In this work, DNA nanotube (DNT) was chosen as a preferred carrier of AMPs owing to its high binding affinity and loading capacity, and an engineered broad-spectrum AMP named RP557 was screened as the cargo though molecular simulation and subsequent loading experiments. RP557 molecules were then loaded onto DNT through electrostatic interaction to construct RP557@DNT nanocomplex for improved anti-infective therapy. Loaded RP557 possessed the lower cytotoxicity to fibroblasts and epithelial cells and the higher compatibility to red blood cells relative to free RP557 <em>in vitro</em>, and RP557@DNT displayed the highly favored biodegradability and biosafety at the animal level. In addition, compared to free RP557, RP557@DNT endowed better bactericidal activity <em>in vitro</em> and <em>in vivo</em> because loaded RP557 exhibited higher resistance to serum protease degradation and controlled release onto bacterial cell membrane. The high therapeutic effect of RP557@DNT primarily depended on the acceleration of inflammation resolution (involving the reduction in proinflammatory factor production, innate immune cell recruitment, and adaptive immunity) and tissue repair (involving the up-regulation of multiple epidermal and dermal repair pathways). In summary, RP557@DNT showed significantly enhanced anti-enzymolysis, antibacterial activity, and biosafety relative to free RP557, and thus it represented a high-efficiency antibiotics-alternative strategy for treating refractory infections.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102508"},"PeriodicalIF":13.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen nanobubbles enhancing antioxidant activity of glutathione peroxidase: Superiority at the nanoscale over molecular scale 氢纳米气泡增强谷胱甘肽过氧化物酶的抗氧化活性：纳米尺度优于分子尺度

IF 13.2 1区材料科学

Nano Today Pub Date : 2024-09-26 DOI: 10.1016/j.nantod.2024.102510

You Zhang , Xiaofeng Wang , Shu Liu , Jing Wang , Pingping Zheng , Damin Xu , Qian Liu , Liming Wang , Wenhong Fan , Fengchang Wu

{"title":"Hydrogen nanobubbles enhancing antioxidant activity of glutathione peroxidase: Superiority at the nanoscale over molecular scale","authors":"You Zhang , Xiaofeng Wang , Shu Liu , Jing Wang , Pingping Zheng , Damin Xu , Qian Liu , Liming Wang , Wenhong Fan , Fengchang Wu","doi":"10.1016/j.nantod.2024.102510","DOIUrl":"10.1016/j.nantod.2024.102510","url":null,"abstract":"<div><div>As novel antioxidants, hydrogen nanobubbles (NBs) intricately regulate the growth and developmental processes of organisms, bolstering their tolerance to external stresses. Despite their recognized potential, the precise antioxidative mechanisms remain inadequately elucidated. In this study, we present evidence supporting the protective role of hydrogen NBs in an oxidative stress system, utilizing Cu<sup>2+</sup> as a prototypical inducer and <em>Tetrahymena thermophila</em> as a representative model organism. To elucidate the molecular mechanism of this phenomenon, we employed a comprehensive approach, integrating transcriptomic analysis and molecular dynamics simulations. Additionally, intrinsic differential scanning and surface plasmon resonance techniques were applied to unveil the molecular-level response and nanoscale interactions. Our investigation revealed that hydrogen NBs induce a notable upregulation in the expression of glutathione peroxidase (GPx). Moreover, compared to molecular hydrogen, hydrogen NBs have a more pronounced effect on the structural reconfiguration and catalytic efficacy of GPx, as demonstrated by the greater reduction in the distance between the catalytic center amino acids and a significant increase in GPx’s affinity for GSH. In summary, our findings underscore GPx as the targeted molecules through which hydrogen NBs exert their antioxidative effects. These insights contribute to a deeper comprehension of the molecular implications of hydrogen NBs and provide new perspectives for alleviating the toxicity of environmental pollutants.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"59 ","pages":"Article 102510"},"PeriodicalIF":13.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0