Nano Today最新文献

{"title":"In situ nanometer-resolution strain and orientation mapping for gas-solid reactions via precession-assisted four-dimensional scanning transmission electron microscopy","authors":"Yongwen Sun ,&nbsp;Ying Han ,&nbsp;Dan Zhou ,&nbsp;Athanassios S. Galanis ,&nbsp;Alejandro Gomez-Perez ,&nbsp;Ke Wang ,&nbsp;Stavros Nicolopoulos ,&nbsp;Hugo Pérez-Garza ,&nbsp;Yang Yang","doi":"10.1016/j.nantod.2025.102784","DOIUrl":"10.1016/j.nantod.2025.102784","url":null,"abstract":"<div><div>Chemomechanical interactions in gas or liquid environments are crucial for the functionality and longevity of various materials used in sustainable energy technologies, such as rechargeable batteries, water-splitting catalysts, and next-generation nuclear reactors. A comprehensive understanding of nanoscale strain evolution involved in these processes can advance our knowledge of underlying mechanisms and facilitate material design improvements. However, traditional microscopy workflows face challenges due to trade-offs between field of view (FOV), spatial resolution, temporal resolution, and electron beam damage, particularly in gas or liquid environments. Here, we demonstrate <em>in situ</em> nanometer-resolution strain and orientation mapping in a temperature-controlled gas environment with a large FOV. This is achieved by integrating a microelectromechanical system (MEMS)-based closed-cell TEM holder, precession-assisted four-dimensional scanning transmission electron microscopy (4D-STEM), and a direct electron detector (DED). Using the strain evolution during zirconium initial oxidation as a case study, we first outline critical strategies for focused ion beam (FIB) gas-cell sample preparation and gas-phase TEM workflows to enhance experimental success. We then show that integrating DED with precession electron diffraction (PED) and optimizing gas pressure substantially improve the quantity and quality of the detected Bragg peaks in nano-beam electron diffraction (NBED) patterns, enabling more precise strain and orientation measurements. Furthermore, we introduce a practical protocol to pause the reactions, allowing sufficient time for 4D-STEM data collection while ensuring the temporal resolution needed to resolve material dynamics. Our methodology and workflow provide a robust framework for quantitative analysis of chemomechanical evolutions in materials exposed to gas or liquid environments, paving the way for improved material design in energy-related applications.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102784"},"PeriodicalIF":13.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiphase flow-enabled microfluidic sorting reveals cellular chain length-dependent pathogenic heterogeneity","authors":"Yike Li ,&nbsp;Ziwei Han ,&nbsp;Ludan Zhang ,&nbsp;Ya Zhao ,&nbsp;Yan Li ,&nbsp;Shaokun Dong ,&nbsp;Liqin Zhang ,&nbsp;Chao Yuan ,&nbsp;Yuguang Wang ,&nbsp;Chao Liu ,&nbsp;Jiashu Sun","doi":"10.1016/j.nantod.2025.102802","DOIUrl":"10.1016/j.nantod.2025.102802","url":null,"abstract":"<div><div>Delineating morphology-related pathogenic heterogeneity of micro- and nano-sized pathogens remains challenging due to the absence of precise and gentle sorting methods. Here, we report a multiphase flow-enabled microfluidic approach for label-free, high-resolution sorting of <em>Streptococcus mutans</em> (<em>S. mutans</em>) based on cellular morphology. By co-flowing viscoelastic fluid (<em>S. mutans</em> spiked with polyethylene oxide, PEO) and Newtonian fluid (PBS) in the microchannel, the synergistic effect of elastic and inertial lift forces leads to chain length-dependent migration and sorting of <em>S. mutans</em> (0.5 μm in diameter) into short- (0.6–2 μm), medium- (2–4.5 μm), and long-chain (4.5–15 μm) subpopulations, achieving separation efficiencies &gt; 77.8 %, purities &gt; 85 %, and viabilities &gt; 98.5 %. Notably, we reveal that the cellular chain length is a critical factor for pathogenicity of <em>S. mutans</em>. The long-chain subpopulation exhibits enhanced biofilm formation, stronger resistance to phagocytosis, and higher MIC₅₀ of antibacterials compared to medium- and short-chain subpopulations. Our microfluidic sorting method offers a new tool for studying pathogenic heterogeneity.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102802"},"PeriodicalIF":13.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acid-tolerant and hypoxia-responsive drug delivery system based on sulphobetaine-modified azocalix[4]arene for treating ulcerative colitis","authors":"Jing Liu ,&nbsp;Juan-Juan Li ,&nbsp;Hai-Qing Li ,&nbsp;Meiqi Yang ,&nbsp;Kai Zhang ,&nbsp;Fangqian Yin ,&nbsp;Yanbin Ren ,&nbsp;Mengqiong Ming ,&nbsp;Dong-Sheng Guo ,&nbsp;Ke-Rang Wang","doi":"10.1016/j.nantod.2025.102801","DOIUrl":"10.1016/j.nantod.2025.102801","url":null,"abstract":"<div><div>Oral administration has been considered a conventional therapeutic strategy for ulcerative colitis (UC) due to better safety profiles, convenience of administration and high patient compliance. However, the application faces challenges of premature drug release and degradation in the harsh gastrointestinal environment, resulting in a diminished therapeutic efficacy. Herein, we customized an acid-stable and hypoxia-responsive macrocyclic carrier, zwitterionic sulphobetaine modified azocalix[4]arene (SBAC4A). SBAC4A possesses the hypoxia-responsiveness ability due to the azo groups, the acid resistance ability in the acidic environment because of the zwitterionic parts and phenolic-OH groups, and the capacity for effective internalization by colon epithelial cells on account of its zwitterionic nature. By loading sulfasalazine (SF), the resultant supramolecular nanoformulation SF@SBAC4A demonstrates enhanced anti-inflammatory efficacy. When applied in UC in vivo, the therapeutic effect of SF@SBAC4A was significantly enhanced compared to free SF, as it effectively alleviated colon inflammation and promoted mucosal repairment. Importantly, this design principle provides a supramolecular strategy for developing oral delivery systems for anti-inflammatory drugs.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102801"},"PeriodicalIF":13.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multistage microRNA nanotherapeutic to address fibrosis of bacterial keratitis","authors":"Rui Zhao ,&nbsp;Yujing Zheng ,&nbsp;Kang Xu ,&nbsp;Linwei Huang ,&nbsp;Jiayi Ding ,&nbsp;Xunjie Shang ,&nbsp;Xiangyu Tao ,&nbsp;Songlin Xin ,&nbsp;Qinxiang Zheng ,&nbsp;Yuna Qian ,&nbsp;Jianliang Shen","doi":"10.1016/j.nantod.2025.102800","DOIUrl":"10.1016/j.nantod.2025.102800","url":null,"abstract":"<div><div>Bacterial keratitis (BK) is a leading cause of visual impairment and blindness. The conventional treatment involves frequent instillation of antibiotics at the lesion site. However, this approach not only leads to bacterial resistance but also suffers from poor bioavailability and often overlooks the damage caused by secondary scarring following corneal infections. In this study, we have developed a multistage nanotherapeutic (DHA-lys@miRNA) to address both anti-infection and secondary scarring hyperplasia during the treatment of bacterial keratitis. Specifically, docosahexaenoic acid (DHA) and ε-polylysine (ε-PL) were successfully self-assembled to form an amphipathic cationic nanocarrier, DHA-lys, which can complex with microRNA to form nanotherapeutics. This nanosystem exhibits potent bacteriostatic effects and amphiphilicity, enabling it to bind to the tear film lipid layer and conjunctival mucosal layer, thereby significantly enhancing its residence time and penetration in the corneal tissue. This, in turn, improves the bioavailability of the drug in bacterial keratitis. Furthermore, we have identified miR-361–3p as a key microRNA through database screening and a series of experiments. This microRNA can specifically inhibit corneal fibrosis by interrupting the crosstalk between corneal stromal cells and myofibroblasts in a BK mouse model <em>in vivo</em>. Overall, this study offers innovative insights into the treatment of infectious eye diseases, leveraging the potential of nucleic acid nanotherapeutics.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102800"},"PeriodicalIF":13.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuning planar and selective Zn evolution promoted by densely grown covalent organic framework towards high performance zinc batteries","authors":"Tiancun Liu ,&nbsp;Ronghan Jiang ,&nbsp;Ling Chen ,&nbsp;Song Lu ,&nbsp;Xiqing Mai ,&nbsp;Xueqin Zhang ,&nbsp;Yi Xu ,&nbsp;Zhixin Yu ,&nbsp;Yong Wang","doi":"10.1016/j.nantod.2025.102806","DOIUrl":"10.1016/j.nantod.2025.102806","url":null,"abstract":"<div><div>Zn dendrites generation and excess electrolyte consumption prevent the practical application of aqueous Zn batteries (AZBs). Herein, zincophilic separators (glass fibers@ covalent organic framework, GF@COF) are synthesized via a facile one-step solvothermal method based on Schiff base reaction. Specifically, the separator surface is densely decorated by in situ grown covalent organic framework (COF). Zincophilic N and O containing groups in COF exhibit excellent binding capability with Zn ions, driving homogenous Zn distribution and boosting Zn-ion flux. COF can promote shielding of (100) and (101) plane of Zn metal and directional exposure of (002) plane, steering selective and planar Zn deposition along (002) plane. The mechanism of achieving non-dendrite growth and reduced electrolyte loss has been confirmed by in situ XRD, in situ FT-IR and theoretical calculation. Electrochemical performances of ultrastable rate capability of 4500 h at ambient condition and high reversible capacity of 78.6 mAh g⁻¹ (capacity retention of 94 %) even after 2000 cycles at a large current of 5 A g⁻¹ for MnO₂ based full cell are observed, which are substantially superior than previous literature.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102806"},"PeriodicalIF":13.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferritin-based targeted delivery of cimifugin to hair cells provides strong protection against hearing loss","authors":"Junying Zhang ,&nbsp;Shengda Cao ,&nbsp;Ming Yang ,&nbsp;Jiaru Zhang ,&nbsp;Haibing Huang ,&nbsp;Zhiwei Zheng ,&nbsp;Kelong Fan ,&nbsp;Xiyun Yan ,&nbsp;Guohui Nie ,&nbsp;Hui Ding ,&nbsp;Yingzi He ,&nbsp;Xiaojun Wang","doi":"10.1016/j.nantod.2025.102790","DOIUrl":"10.1016/j.nantod.2025.102790","url":null,"abstract":"<div><div>Deafness and hearing loss are the most widely distributed sensory organ disabilities worldwide, and their negative impact places them at the top of the global burden of diseases list. Therefore, the need to develop new and efficient prevention and treatment strategies is urgent. Here, we successfully developed a noninvasive drug delivery system involving a ferritin (Fn)-based drug delivery platform. To further increase therapeutic efficacy, we loaded cimifugin (Ci), an active ingredient extracted from <em>Cimicifuga racemosa</em>, into Fn to form the complex Ci@Fn. Ci@Fn was injected through the retroauricular round window membrane and then transported to the basement membrane along with the flow of ectolymphatic and endolymphatic fluids. Further through the targeting function of Fn, Ci@Fn was able to precisely target hair cells (HCs). Subsequently, Ci@Fn is endocytosed by HCs and releases Ci in lysosomes, exerting its protective effect on HCs. Neonatal mouse model experiments revealed that Ci@Fn significantly reduced cisplatin (Cis)-induced cochlear hair cell damage and prevented hair cell loss and apoptosis. In addition, RNA sequencing analysis revealed that Ci@Fn attenuates Cis-induced ototoxicity mainly by modulating the PI3K-Akt signaling pathway. Finally, in validation experiments in an adult mouse model of noise-induced hearing damage, we found that Ci@Fn had a significant hearing-protective effect, superior to the current gold standard treatment (dexamethasone). These findings demonstrate that Ci@Fn is an innovative and efficient solution for hearing loss treatment and lays a solid foundation for future clinical applications.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102790"},"PeriodicalIF":13.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphdiyne oxide in polymer electrolytes: Synergistic non-covalent interactions boosting Li-ion transport and LiF-rich solid electrolyte interface","authors":"Kaihang Wang ,&nbsp;Chao Jiang ,&nbsp;Luwei Zhang ,&nbsp;Ru Li ,&nbsp;Ze Yang ,&nbsp;Chunfang Zhang ,&nbsp;Ning Wang","doi":"10.1016/j.nantod.2025.102803","DOIUrl":"10.1016/j.nantod.2025.102803","url":null,"abstract":"<div><div>Solid-state polymer electrolytes (SPEs) face challenges including low lithium-ion transference numbers (t_Li⁺) and uncontrolled lithium dendrite growth. Herein, a design concept for the synergic regulation of the non-covalent interactions within SPEs is proposed. Graphdiyne oxide (GDYO), featuring abundant carboxyl groups, is incorporated into polyethylene oxide (PEO)-based SPEs. GDYO engages in hydrogen bonding with PEO and electrostatic interactions with LiTFSI, facilitating the transfer of Li⁺ in SPEs. Furthermore, a LiF-rich solid electrolyte interphase (SEI) is formed, effectively suppressing dendrite formation. The optimized GDYO/PEO SPE achieves a high t_Li⁺ of 0.75 and stable Li plating/stripping over 1300 h in Li||Li symmetric cells. GDYO/PEO SPE enables coin full cells to exhibit superior cycling stability, rate performance, and high-voltage suitability, while also offering superior safety and flexibility for pouch cells. Our results highlight the pivotal role of synergistic non-covalent interactions in SPEs, providing a novel strategy for improving the performance of energy storage batteries.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102803"},"PeriodicalIF":13.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perivascular delivery of photothermal-immunotherapy nanovaccine bypasses blood circulation to enhance the antitumor immune response of breast cancer","authors":"Nan Hu ,&nbsp;Yuling Liu ,&nbsp;Yilong Du ,&nbsp;Yanxia Gao ,&nbsp;Yinghan Wang ,&nbsp;Huizhen Sun ,&nbsp;Yanbin Meng ,&nbsp;Dong Han ,&nbsp;Xiaoli Shi","doi":"10.1016/j.nantod.2025.102798","DOIUrl":"10.1016/j.nantod.2025.102798","url":null,"abstract":"<div><div>Nanovaccines, with the synergistic effect of photothermal-immunotherapy, are one of the most advanced approaches for improving antitumor outcomes. However, targeted nanovaccine delivery to the tumor and draining lymph nodes remain critical obstacles due to the complex tumor microenvironment. Herein, we discovered a new drug delivery route of intervaginal space injection (ISI) to target tumor tissue and draining lymph nodes to bypass blood circulation. The synthesized nanoplatform (PDA/ICG/CpG, PIC) was integrated with polydopamine (as a photosensitizer), indocyanine green (as the imaging probe), and CpG ODN (as the immune adjuvant) for fluorescence imaging-guided photothermal-immunotherapy. After being injected via ISI, the nanovaccine accumulated excellently in the tumor and drained the lymph node, owing to the integrity of the interstitial structure and fluid flow. The tumor microenvironment, which has severely hindered drug delivery in recent research, may facilitate transportation via ISI. Under laser irradiation, tumor cells were killed by hyperthermia, whereby tumor-related antigens were released to promote the maturation of dendritic cells (DCs), evoking T lymphocyte activation. Aided by CpG, DC maturation, and CD8⁺ T-cell infiltration into the tumor, draining lymph nodes and spleen were significantly amplified. PDA/ICG/CpG injected via the interstitial space of the carpal tunnel effectively inhibited tumor growth and metastasis in a 4T1-bearing mammary model. This study provided different insights into targeted drug delivery. Nanovaccines with photothermal-immunotherapy of a suitable size that is administered via ISI have the potential to enable precise cancer treatment.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102798"},"PeriodicalIF":13.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhance efferocytosis capacity of dendritic cells for diabetic wound healing by nanozyme-loaded nanofiber dressings","authors":"Danfeng Jian ,&nbsp;Kai Ye ,&nbsp;Limo Wang ,&nbsp;Lulu Jin ,&nbsp;Huang Yang ,&nbsp;Lie Ma ,&nbsp;Chenggang Yi ,&nbsp;Zhengwei Mao ,&nbsp;Jindan Wu","doi":"10.1016/j.nantod.2025.102787","DOIUrl":"10.1016/j.nantod.2025.102787","url":null,"abstract":"<div><div>Improving efferocytosis of dendritic cells (DCs) by inhibiting membrane transporter protein SLC7A11 has been explored for healing diabetes wounds. However, the administration of SLC7A11 inhibitors has been linked to the generation of reactive oxygen species (ROS), which may compromise the viability and functionality of DCs. To mitigate this adverse effect, a nanozyme-loaded nanofiber dressing, termed GIPH, was developed. This dressing encapsulates a specific SLC7A11 inhibitor (HG106) in the core layer and incorporates an efficient nanozyme, Ir NPs-PVP, in the shell layer. The Ir NPs-PVP nanozyme effectively neutralizes ROS produced by HG106, thereby enhancing the viability of DCs and further promoting their efferocytosis. Our <em>in vitro</em> experiment results demonstrated that GIPH-treated Bone Marrow-Derived Dendritic Cells (BMDCs) exhibited a 1.68-fold increase in survival and a 1.52-fold enhancement in efferocytosis rate compared to those treated with HG106 alone. Furthermore, in a diabetic mouse wound model, the GIPH group showed superior healing outcomes, with wound nearly achieving complete recovery by day 12 (97.5 %). This research highlights the therapeutic potential of nanozyme-loaded nanofiber dressings while providing valuable insights for future investigations into the DCs-mediated wound healing process.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102787"},"PeriodicalIF":13.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered bacteria with inserted CAR cell membranes for enhanced tumor targeting and biotherapy","authors":"Si-Min Zeng,&nbsp;Ke-Wei Chen,&nbsp;Ting Pan,&nbsp;Yun-Xia Sun,&nbsp;Jun Feng,&nbsp;Xian-Zheng Zhang","doi":"10.1016/j.nantod.2025.102799","DOIUrl":"10.1016/j.nantod.2025.102799","url":null,"abstract":"<div><div>Although bacteria have been widely applied in treating various tumors, their inadequate tumor-specific targeting and in vivo controllability often result in suboptimal therapeutic outcomes and inevitable side effects post-administration. Here, we develop an acid-responsive engineered bacteria system (CARM-Bac^ClyA) with inserted chimeric antigen receptor (CAR) cell membranes (M) for enhanced tumor targeting and biotherapy. Specifically, we genetically engineer attenuated <em>Escherichia coli</em> to construct acid-responsive therapeutic bacteria (Bac^ClyA) that express cytotoxic cytolysin A (ClyA) proteins. Simultaneously, we genetically engineer cells to construct cells that express CARs specifically targeting tumor antigens. Subsequently, CARM fragments are inserted into Bac^ClyA during plasmolysis and deplasmolysis (P/deP) to obtain CARM-Bac^ClyA. As a proof-of-concept study, in the mouse solid B-cell lymphoma model, the enrichment of CARM-Bac^ClyA at tumor sites is significantly increased to 2.5-fold that of naked Bac^ClyA after systemic administration. Furthermore, the acidic pH triggers Bac^ClyA to express ClyA proteins, leading to perforation-induced tumor cell death, thereby inhibiting tumor growth. This work introduces a strategy for manipulating engineered bacterial behaviors, and proves the potential of CARM-inserted engineered bacteria for enhanced tumor targeting and biotherapy.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"64 ","pages":"Article 102799"},"PeriodicalIF":13.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}