Yike Li , Ziwei Han , Ludan Zhang , Ya Zhao , Yan Li , Shaokun Dong , Liqin Zhang , Chao Yuan , Yuguang Wang , Chao Liu , Jiashu Sun
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引用次数: 0
Abstract
Delineating morphology-related pathogenic heterogeneity of micro- and nano-sized pathogens remains challenging due to the absence of precise and gentle sorting methods. Here, we report a multiphase flow-enabled microfluidic approach for label-free, high-resolution sorting of Streptococcus mutans (S. mutans) based on cellular morphology. By co-flowing viscoelastic fluid (S. mutans spiked with polyethylene oxide, PEO) and Newtonian fluid (PBS) in the microchannel, the synergistic effect of elastic and inertial lift forces leads to chain length-dependent migration and sorting of S. mutans (0.5 μm in diameter) into short- (0.6–2 μm), medium- (2–4.5 μm), and long-chain (4.5–15 μm) subpopulations, achieving separation efficiencies > 77.8 %, purities > 85 %, and viabilities > 98.5 %. Notably, we reveal that the cellular chain length is a critical factor for pathogenicity of S. mutans. The long-chain subpopulation exhibits enhanced biofilm formation, stronger resistance to phagocytosis, and higher MIC50 of antibacterials compared to medium- and short-chain subpopulations. Our microfluidic sorting method offers a new tool for studying pathogenic heterogeneity.
期刊介绍:
Nano Today is a journal dedicated to publishing influential and innovative work in the field of nanoscience and technology. It covers a wide range of subject areas including biomaterials, materials chemistry, materials science, chemistry, bioengineering, biochemistry, genetics and molecular biology, engineering, and nanotechnology. The journal considers articles that inform readers about the latest research, breakthroughs, and topical issues in these fields. It provides comprehensive coverage through a mixture of peer-reviewed articles, research news, and information on key developments. Nano Today is abstracted and indexed in Science Citation Index, Ei Compendex, Embase, Scopus, and INSPEC.