Biopolymers and Cell最新文献_第6页

Cross-species differential plasma protein binding of Propoxazepam, a novel analgesic agent. 新型镇痛剂丙泊酚的跨物种差异血浆蛋白结合。

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a68

M. Golovenko, A. Reder, V. Larionov, S. A. Andronati, A. S. Akisheva

引用次数: 0

Development of receptor-based protein kinase Cβ (PKCβ) pharmacophore model for the search of inhibitors with potential activity against acute respiratory distress syndrome (ARDS) 基于受体的蛋白激酶Cβ（PKCβ）药效团模型的建立用于寻找对急性呼吸窘迫综合征（ARDS）具有潜在活性的抑制剂

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a6b

S. Starosyla, G. Volynets, M. Protopopov, I. Koleiev, V. Bdzhola, S. Yarmoluk

引用次数: 0

Zearalenone-selective biomimetic-based sensor system and its validation for real samples’ analysis 玉米赤霉烯酮选择性仿生传感器系统及其在实际样品分析中的验证

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a69

D. Yarynka, T. Sergeyeva, E. Piletska, Yevgeny Stepanenko, O. Brovko, S. A. Piletsky, A. El'skaya

引用次数: 1

Benzoxazole styrylcyanine dye as a fluorescent probe for functional amyloid visualization in Staphylococcus aureus ATCC25923 biofilm 苯并恶唑苯乙烯花青染料作为荧光探针在金黄色葡萄球菌ATCC25923生物膜中观察功能性淀粉样蛋白

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a6a

S. Chernii, O. Moshynets, D. Aristova, D. V. Kryvorotenko, M. Losytskyy, O. S. Iungin, S. Yarmoluk, G. Volynets

引用次数: 0

Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines 乌克兰面包小麦品种和品系GLI-B1基因座的遗传变异

Biopolymers and Cell Pub Date : 2021-10-30 DOI: 10.7124/bc.000a63

Yulia Popovych, O. M. Blagodarova, S. Chebotar

{"title":"Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines","authors":"Yulia Popovych, O. M. Blagodarova, S. Chebotar","doi":"10.7124/bc.000a63","DOIUrl":"https://doi.org/10.7124/bc.000a63","url":null,"abstract":"Aim. To investigate polymorphism of Gli-B1 locus in modern Ukrainian bread wheat cultivars, to analyze the distribution of alleles and to compare the received data with the “core-collection of wheat cultivars” presented by Dr. E. Metakovsky. Methods. Eighty one bread wheat cultivars and lines from different plant breeding institutions and stations of Ukraine were tested using allele-specific primers to Gli-B1 locus developed by Zhang et al. [2003]. PCR products were fractionated in polyacrylamide gel (PAG) and then were stained by silver nitrate. Allelic variants of gliadins were analyzed by electrophoresis in acid polyacrylamide gel (APAGE). Results. Nine allelic variants of gliadins were revealed by APAGE and six alleles of Gli-B1 locus were detected by PCR-analysis. In 52 % of modern Ukrainian bread wheat cultivars we revealed Gli-B1b allelic variant, according to PCR - Gli-B1.1 allele with a 369 bp amplification fragment. In the genotypes of Ukrainian wheat cultivars, the 1RS.1BL translocation, which carries resistance genes, is frequent, as was detected by the absence of any amplification fragments with Gli-B1 primers. The correspondence between allelic variants of gliadins and alleles of Gli-B1 locus is discussed. Conclusions. DNA polymorphism of Gli-B1 locus examined in our research coincides with the diversity of allelic variants of gliadins, which were detected by APAGE method for Ukrainian bread wheat cultivar. However, PCR-analysis with applied primers carried out in this study does not distinguish the alleles that correspond to the Gli-B1c, Gli-B1g and Gli-B1e allelic variants of gliadins. The most common allele (52 %) for the in-vestigated Ukrainian wheat varieties is Gli-B1.1 allele, which was characterized by the amplification fragment of 369 bp, and the presence of 1RS.1BL translocation, which corresponds to Gli-B1b and Gli-B1l allelic variants of gliadins obtained by APAGE, respectively.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41645511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination 褪黑素和成纤维细胞生长因子-2增强人脐带多能间充质基质细胞对铜硫酮诱导的小鼠脱髓鞘的影响

Biopolymers and Cell Pub Date : 2021-10-30 DOI: 10.7124/bc.000a62

I. Labunets, N. Utko, O. Toporova, S. Savosko, I. Pokholenko, T. Panteleymonova, G. Butenko

{"title":"Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination","authors":"I. Labunets, N. Utko, O. Toporova, S. Savosko, I. Pokholenko, T. Panteleymonova, G. Butenko","doi":"10.7124/bc.000a62","DOIUrl":"https://doi.org/10.7124/bc.000a62","url":null,"abstract":"Aim. To study the influence of melatonin and recombinant human fibroblast growth factor (rhFGF-2) on human umbilical cord multipotent mesenchymal stromal cells (hMMSCs) effects at experimental demyelination. Methods. Adult mice were fed with neurotoxin cuprizone for 3 weeks. hMMSCs (5 × 10 5 cells) were injected on the 10th day of cuprizone diet. Injections of melatonin or rhFGF-2 were started on the 11th day of cuprizone diet. We used cell culture, flow cytometric, spectrophotometric and histological methods, “open field” and “rotarod” tests. Results. Under the cuprizone influence the motor-, emotional activities and muscle tone decreased. The malondialdehyde (MDA) content in brain increased while the activity of antioxidant enzymes decreased. After injection of hMMSCs the number of crossed squares and grooming activity increased while MDA content decreased. Melatonin and rhFGF-2 injections enhanced the effect of cells on grooming activity and increased the glutathione reductase activity. Melatonin also increased the number of boluses, muscle tone and glutathione peroxidase activity. Conclusion. Melatonin and rhFGF-2 improve the effect of hMMSCs in cuprizone-treated mice. The effects of hMMSCs and melatonin combination is greater than that with rhFGF-2.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43732207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characteristics of regenerated keratin and keratin-based film 再生角蛋白及其基膜的特性

Biopolymers and Cell Pub Date : 2021-10-30 DOI: 10.7124/bc.000a5f

V. Havryliak, V. Mykhaliuk, V. Kochubei

引用次数: 0

Validation of aflatoxin B1 MIP membrane-based smartphone sensor system for real sample applications 黄曲霉毒素B1 MIP膜智能传感器系统在实际样品应用中的验证

Biopolymers and Cell Pub Date : 2021-10-30 DOI: 10.7124/bc.000a60

D. Yarynka, T. Sergeyeva, E. Piletska, R. Linnik, M. Antonyuk, O. Brovko, S. A. Piletsky, A. El'skaya

{"title":"Validation of aflatoxin B1 MIP membrane-based smartphone sensor system for real sample applications","authors":"D. Yarynka, T. Sergeyeva, E. Piletska, R. Linnik, M. Antonyuk, O. Brovko, S. A. Piletsky, A. El'skaya","doi":"10.7124/bc.000a60","DOIUrl":"https://doi.org/10.7124/bc.000a60","url":null,"abstract":"Aim. To calibrate and validate of innovative MIP membrane-based smartphone sensor system for aflatoxin B1 detection in real cereal samples. Methods. Porous molecularly imprinted polymer (MIP) membranes were synthesized according to the technique of molecular imprinting using the method of in situ polymerization. As a dummy template, ethyl-2-oxocyclopentanecar-boxylate was used. Acrylamide was used as a functional monomer according to the results of molecular dynamics. Porous 60 µm-thick MIP membranes were obtained using UV polymerization for 30 min (Sergeyeva et al. 2017). The Fluorescent sensor responses of AFB1 binding to the selective MIP membrane surface were recorded by the spectrofluorimeter as well as smartphone camera and further analyzed by smartphone application Spotxel® Reader (Sicasys Software GmbH, Germany). Results. The calibration plots for both MIP membrane-based fluorescent sensor system and smartphone sensor system were obtained. The possibility of successful application of the proposed sensor systems to analyze AFB1 in real samples was demonstrated. Conclusions. The MIP membrane-based smartphone sensor was calibrated and validated for reliable and robust aflatoxin B1 detection in the real cereal samples. The proposed MIP membrane-based smartphone sensor system provides affordable, sensitive, and equipment-free technique for AFB1 analysis compared to the traditional analytical methods.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49270456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration 治疗性反义寡脱氧核苷酸与阳离子聚合物复合物对细胞呼吸的影响

Biopolymers and Cell Pub Date : 2021-10-30 DOI: 10.7124/bc.000a61

M. Kozak, D. Ostapiv, N. Mitina, I. Petruh, K. Volianiuk, A. Zaichenko, V. Vlizlo

{"title":"An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration","authors":"M. Kozak, D. Ostapiv, N. Mitina, I. Petruh, K. Volianiuk, A. Zaichenko, V. Vlizlo","doi":"10.7124/bc.000a61","DOIUrl":"https://doi.org/10.7124/bc.000a61","url":null,"abstract":"Antisense-DNA technologies are new strategy for the treatment of prion infections. This stra-te gy requires prolonged administrations of the drugs, which are likely to alter cell redox processes. Aim. The evaluation of cell survival and intensity of oxidative processes in vitro under the influence of antisense-oligodeoxynucleotides (asODNs) as cell prion inhibitors (PrP C ) complexed with cationic polyelectrolyte. Methods. Free diffusion in agarose gel, study of cytotoxic action on model cells (bull semen), polarography and potentiometric measurement of oxygen uptake, statistical analysis. Results. Poly(dimethylaminoethyl methacrylate)[-]based surfactants form complexes with asODNs. Polyethylene glycol containing surfactants increase oxygen uptake by cells: by 18 % ( VI ), by 37 % ( IV ) and 2.6-fold for V . An addition of the IV -asODNs complex into [the] cell medium did not affect the oxygen absorption; however, it increased reduction processes. Interpolyelectrolyte complex V -asODNs increased the cell respiration by 1.95 times. VI separately increased the cell absorption of oxygen by 18 % and in the complex with asODNs — by 36 %. VI possessed the lowest cytotoxicity. Conclusions. New cationic polyelectrolytes form complexes with asODNs. VI causes the smallest effect on the RedOx processes of model cells and possesses the lowest cytotoxic effect.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71341839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and evaluation of biological activity of 1-[2-amino-4-methylthiazol-5-yl]-3-arylpropenones 1-[2-氨基-4-甲基噻唑-5-基]-3-芳基丙烯的合成及生物活性评价

Biopolymers and Cell Pub Date : 2021-10-30 DOI: 10.7124/bc.000a64

A. Lozynskyi, I. Yushyn, Y. Konechnyi, O. Roman, O. V. Matiykiv, O. Smaliukh, L. Mosula, S. Polovkovych, R. Lesyk

{"title":"Synthesis and evaluation of biological activity of 1-[2-amino-4-methylthiazol-5-yl]-3-arylpropenones","authors":"A. Lozynskyi, I. Yushyn, Y. Konechnyi, O. Roman, O. V. Matiykiv, O. Smaliukh, L. Mosula, S. Polovkovych, R. Lesyk","doi":"10.7124/bc.000a64","DOIUrl":"https://doi.org/10.7124/bc.000a64","url":null,"abstract":"Aim. To accomplish the synthesis and screening of anticancer and antimicrobial activities of 1-[2-amino-4-methylthiazol-5-yl]-3-arylpropenones 2-10. Methods. The in vitro anticancer activity of compounds 4, 6, 8-10 has been established by DTP(Developmental Therapeutics Program) of the National Cancer Institute. The antibacterial and antifungal activities of synthesized thiazole-based derivatives were evaluated in vitro with the agar diffusion and broth microdilution methods to wards Gram-positive, Gram-negative bacteria and yeasts. For the synthesized compounds, the in silico drug-likeness screening using SwissADME online server is reported. Results. The novel 1-[2-amino-4-methylthiazol-5-yl]-3-arylpropenones were synthesized from 1-[2-amino-4-methylthiazol-5-yl]ethanones and various aromatic aldehydes in the Claisen–Schmidt condensation. The synthesized compound 9 was moderately active against the leukemia CCRF-CEM and HL-60(TB), renal cancer UO-31 and breast cancer MCF7 cell lines. The antimicrobial screening led to identification of the active compound 10 against Staphylococcus aureus , Pseudomonas aeruginosa , and Candida albicans . Conclusions. The results obtained herein provide a platform for structure-based optimization of these newly identified thiazole-based compounds for the anticancer and antibacterial drug design.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47621928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1