Biopolymers and Cell最新文献_第5页

Synthesis and biological evaluation of O-acyloximes of 5-chloro-4-formyl- 1H-pyrrol-3-carboxylates as antimicrobial agents 5-氯-4-甲酰基-1H-吡咯-3-羧酸O-酰亚胺类抗菌剂的合成及生物评价

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a72

A. Grozav, V. Chornous, I. Diichuk, S. Kemskyi, N. Yakovychuk, M. Fedoriv, M. Vovk

引用次数: 1

Transformation of the moss (Ceratodon purpureus) with plasmid DNA delivered by novel block-copolymers of the dimethylaminoethyl methacrylate 新型甲基丙烯酸二甲氨基乙酯嵌段共聚物介导的质粒DNA转化苔藓（Ceratodon purpureus）

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a73

N. Finiuk, N. Mitina, O. Lobachevska, A. Zaichenko, R. Stoika

{"title":"Transformation of the moss (Ceratodon purpureus) with plasmid DNA delivered by novel block-copolymers of the dimethylaminoethyl methacrylate","authors":"N. Finiuk, N. Mitina, O. Lobachevska, A. Zaichenko, R. Stoika","doi":"10.7124/bc.000a73","DOIUrl":"https://doi.org/10.7124/bc.000a73","url":null,"abstract":"Aim. To investigate the potential of poly(2-dimethylamino)ethyl methacrylate (DMAEMA)-based block-like polymers to serve as gene delivery carriers in moss Ceratodon purpureus (Hedw.) Brid. protoplasts, and to evaluate the level of their phytotoxicity. Methods. Organic synthesis; DNA gel retardation assay; adapted PEG-mediated transformation protocol; PCR; light microscopy. Results. The formation of pDNA complex with DMAEMA-based carriers took place at 0.01-0.1 % concentrations of the polymer. The poly-DMAEMA carriers F8-DM1, F8-DM2 (fluorine-containing), LAcr-DM1, LAcr-DM2 (lauryl acrylate-containing), BAcr-DM1, and BAcr-DM2 (butyl acrylate-containing) were effective as carriers of plasmid DNA pSF3 at C. purpureus transformation . PCR analysis confirmed that the transformants of C. purpureus moss contain GFP as a gene of interest after the protoplast transformation by polymers LAcr-DM2, LAcr-DM1, BAcr-DM2, BAcr-DM1 and F8-DM2. The poly-DMAEMA carriers at working concentration (0.0025 %) were relatively non-toxic for protoplasts of C. purpureus moss. 83.1-93.9 % of viable protoplasts of C. purpureus moss were found after the treatment with studied carriers at that dose. However, at 0.25 % i.e. 100 times higher concentration than that used for moss transformation, the poly-DMAEMA carriers reached their IC 50 level. Conclusion. The novel block-like poly-DMAEMA carriers were effective in transformation of C. purpureus moss protoplasts and demonstrated low toxicity.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46534147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrinsic fluorescence of single-tryptophan form of tyrosyl-tRNA synthetase catalytic module with the replacements of Trp 87 and Trp 283 by alanine 用丙氨酸取代Trp 87和Trp 283的单色氨酸形式的酪氨酸- trna合成酶催化模块的固有荧光

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a6d

I. O. Blaschak, V. Zayets, L. A. Kolomiets, A. Kornelyuk

{"title":"Intrinsic fluorescence of single-tryptophan form of tyrosyl-tRNA synthetase catalytic module with the replacements of Trp 87 and Trp 283 by alanine","authors":"I. O. Blaschak, V. Zayets, L. A. Kolomiets, A. Kornelyuk","doi":"10.7124/bc.000a6d","DOIUrl":"https://doi.org/10.7124/bc.000a6d","url":null,"abstract":"Aim. Mammalian tyrosyl tRNA synthetase (TyrRS) is composed of N-terminal catalytic miniTyrRS and non-catalytic C-terminal domain. After cleavage both domains of TyrRS reveal non-canonical cytokine functions. It is important to study the conformational changes of miniTyrRS in the course of ligands binding in different nanocomposite complexes. Fluorescence spectroscopy is a very powerful method to detect the local conformational changes of proteins. The study of single-tryptophan form of the protein can provide important information about flexibility and local conformational changes of the protein functional sites. Methods. Site-directed mutagenesis, bacterial expression, fluorescence spectroscopy. Results. Intrinsic fluorescence characteristics of single-tryptophan Trp40-mini TyrRS were measured, a spectral maximum at 332 nm was revealed, which corresponds to the buried state of Trp40 fluorophore in protein globule. Fluorescence quenching of Trp40 by acrylamide revealed the existence of conformational flexibility of mini TyrRS. Conclusions. Fluorescence studies of the single-tryptophan form of tyrosyl-tRNA synthetase revealed a buried state of Trp40 fluorophore but high conformational flexibility of the enzyme at the nanosecond time scale.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42624500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of chimeric genes in bone marrow cells of children with acute lymphoblastic leukemia upon cytostatic therapy 细胞抑制治疗后儿童急性淋巴细胞白血病骨髓细胞中嵌合基因的表达

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a70

O. Vynnytska, L. Dubey, D. Halytsky

引用次数: 0

The effect of heterocyclic substituent at C-3 position of 1-(4-methyl-piperazin-1-yl)isoquinolines on their anticancer activity 1-(4-甲基哌嗪-1-酰基)异喹啉类化合物C-3位杂环取代基对其抗癌活性的影响

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a71

A. Konovalenko, V. Zhirnov, O. Shablykin, O. Shablykina, V. Moskvina, V. Brovarets

引用次数: 0

The use of probiotic microorganisms in cosmeceuticals 益生菌微生物在药妆中的应用

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a6e

I. Voloshyna, L. Shkotova

引用次数: 0

1-Cycloalkanecarbonyl-substituted thioureas and thiosemicarbazides as effective dihydrofolate reductase inhibitors with antibacterial activity 1-环烷碳羰基取代硫脲和硫代氨基脲作为有效的二氢叶酸还原酶抑制剂具有抗菌活性

Biopolymers and Cell Pub Date : 2022-07-25 DOI: 10.7124/bc.000a6f

O. Kholodniak, M. Tniguer, I. Nosulenko, A. O. Kinichenko, K. I. Kandybey, O. M. Antypenko, S. Kovalenko

{"title":"1-Cycloalkanecarbonyl-substituted thioureas and thiosemicarbazides as effective dihydrofolate reductase inhibitors with antibacterial activity","authors":"O. Kholodniak, M. Tniguer, I. Nosulenko, A. O. Kinichenko, K. I. Kandybey, O. M. Antypenko, S. Kovalenko","doi":"10.7124/bc.000a6f","DOIUrl":"https://doi.org/10.7124/bc.000a6f","url":null,"abstract":"Aim. Search for new antibacterial agents with dihydrofolate reductase-inhibitory activity among N -(R-carbamothiol)cycloalkylcarboxamides using in silico and in vitro methodology, SAR analysis to optimize the synthesis of new potential antinicrobials. Methods. Molecular docking, in vitro DHFR inhibition assay, antimicrobial evaluation, SAR analysis, statistical methods. Results. According to the results of molecular docking to the active center of dihydrofolate reductase (DHFR), namely affinity, the main types of interactions and arrangement in the active center of the enzyme, several N -(R-carbamothioyl)cycloalkylcarboxamides were selected for their inhibitory effect. Based on in vitro screening, few promising compounds with high ability to inhibit DHFR were identified. It was found, that diacylsemicarbazides are more effective inhibitors of DHFR compared to acylthioureas. The studies on antibacterial activity have revealed several promising compounds, namely N -(2-R-hydrazine-1-carbonothioyl)cy-cloalkanecarboxamides, as highly active antimicrobial agents against E. сoli and St. aureus (MIC 3.125–25.0 μg/ml) with high DHFR-inhibitory effect, the activity of which competes with the comparison drug “Nitrofurazone”. This justifies the continuation of systematic research in this direction. Conclusions. A well-founded search among N -(R-carbamothiol)cycloalkyl-carboxamides for new antibacterial agents with dihydrofolate reductase-inhibitory activity, using in silico and in vitro methodology, established relationship between the chemical structure and activity aimed at further design of new potential drug agents.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42374622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein c-phycocyanin, structure, physicochemical and biological properties, methods of extraction 蛋白质c-藻蓝蛋白，结构，理化和生物特性，提取方法

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a66

N. Hudz, V. Turkina, A. Filipska, O. Kuzminov, R. Korytniuk, V. Lubenets, P. Wieczorek, N. Savickienė

引用次数: 0

Donor variability of the Wharton`s jelly-derived MSCs in response to oxidative stress 沃顿果冻来源的骨髓间充质干细胞对氧化应激反应的供体变异性

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a67

M. Kovalchuk, N. Shuvalova, V. Kordium

引用次数: 1

Development of the method of isolation and purification of the recombinant human interleukin-7 重组人白细胞介素-7分离纯化方法的建立

Biopolymers and Cell Pub Date : 2022-03-01 DOI: 10.7124/bc.000a6c

T. Lutsenko, V. Motronenko

引用次数: 0