细胞抑制治疗后儿童急性淋巴细胞白血病骨髓细胞中嵌合基因的表达

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2022-07-25 DOI:10.7124/bc.000a70

O. Vynnytska, L. Dubey, D. Halytsky

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引用次数: 0

摘要

目标研究骨髓细胞中嵌合基因的表达作为儿童急性淋巴细胞白血病（ALL）病程的预后因素，并评估其在细胞抑制化疗耐药性中的作用。方法。通过RT-qPCR测定AF4-MLL、BCR-ABL、E2A-RVX、TEL-AML1-c himeric基因的表达水平。后果AF4-MLL癌基因在缓解期儿童中不表达，所有其他研究的癌基因的表达均处于低水平。在复发的情况下，TEL-AML癌基因没有表达，BCR-ABL高表达。结论。ALL患者的发展、病程和生存在很大程度上取决于骨髓中AF4-MLL、BCR-ABL、E2A-PBX1、TEL-AML嵌合基因的表达；在检测化疗后患者的早期复发时，应考虑到这一点。

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Expression of chimeric genes in bone marrow cells of children with acute lymphoblastic leukemia upon cytostatic therapy

Aim. To study the expression of chimeric genes in bone marrow cells as prognostic factors of the course of acute lymphoblastic leukemia (ALL) in children and [to] evaluate their role in resistance to cytostatic chemotherapy. Methods. The AF4-MLL, BCR-ABL, E2A-RVX, TEL-AML1 c himeric gene expression level was determined by RT-qPCR. Results. The AF4-MLL oncogene was not expressed in children during remission, and the expression of all other studied oncogenes was at a low level. In conditions of relapse, there was no expression of the TEL-AML oncogene and a high expression of the BCR-ABL. Conclusions. The Development, course, and survival of ALL patients largely depend on the expression of AF4-MLL, BCR-ABL, E2A-PBX1, TEL-AML chimeric genes in the bone marrow; this should be taken into account when detecting early relapses in patients after chemotherapy.

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.