Validation of aflatoxin B1 MIP membrane-based smartphone sensor system for real sample applications

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2021-10-30 DOI:10.7124/bc.000a60

D. Yarynka, T. Sergeyeva, E. Piletska, R. Linnik, M. Antonyuk, O. Brovko, S. A. Piletsky, A. El'skaya

{"title":"Validation of aflatoxin B1 MIP membrane-based smartphone sensor system for real sample applications","authors":"D. Yarynka, T. Sergeyeva, E. Piletska, R. Linnik, M. Antonyuk, O. Brovko, S. A. Piletsky, A. El'skaya","doi":"10.7124/bc.000a60","DOIUrl":null,"url":null,"abstract":"Aim. To calibrate and validate of innovative MIP membrane-based smartphone sensor system for aflatoxin B1 detection in real cereal samples. Methods. Porous molecularly imprinted polymer (MIP) membranes were synthesized according to the technique of molecular imprinting using the method of in situ polymerization. As a dummy template, ethyl-2-oxocyclopentanecar-boxylate was used. Acrylamide was used as a functional monomer according to the results of molecular dynamics. Porous 60 µm-thick MIP membranes were obtained using UV polymerization for 30 min (Sergeyeva et al. 2017). The Fluorescent sensor responses of AFB1 binding to the selective MIP membrane surface were recorded by the spectrofluorimeter as well as smartphone camera and further analyzed by smartphone application Spotxel® Reader (Sicasys Software GmbH, Germany). Results. The calibration plots for both MIP membrane-based fluorescent sensor system and smartphone sensor system were obtained. The possibility of successful application of the proposed sensor systems to analyze AFB1 in real samples was demonstrated. Conclusions. The MIP membrane-based smartphone sensor was calibrated and validated for reliable and robust aflatoxin B1 detection in the real cereal samples. The proposed MIP membrane-based smartphone sensor system provides affordable, sensitive, and equipment-free technique for AFB1 analysis compared to the traditional analytical methods.","PeriodicalId":39444,"journal":{"name":"Biopolymers and Cell","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biopolymers and Cell","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7124/bc.000a60","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 2

Abstract

Aim. To calibrate and validate of innovative MIP membrane-based smartphone sensor system for aflatoxin B1 detection in real cereal samples. Methods. Porous molecularly imprinted polymer (MIP) membranes were synthesized according to the technique of molecular imprinting using the method of in situ polymerization. As a dummy template, ethyl-2-oxocyclopentanecar-boxylate was used. Acrylamide was used as a functional monomer according to the results of molecular dynamics. Porous 60 µm-thick MIP membranes were obtained using UV polymerization for 30 min (Sergeyeva et al. 2017). The Fluorescent sensor responses of AFB1 binding to the selective MIP membrane surface were recorded by the spectrofluorimeter as well as smartphone camera and further analyzed by smartphone application Spotxel® Reader (Sicasys Software GmbH, Germany). Results. The calibration plots for both MIP membrane-based fluorescent sensor system and smartphone sensor system were obtained. The possibility of successful application of the proposed sensor systems to analyze AFB1 in real samples was demonstrated. Conclusions. The MIP membrane-based smartphone sensor was calibrated and validated for reliable and robust aflatoxin B1 detection in the real cereal samples. The proposed MIP membrane-based smartphone sensor system provides affordable, sensitive, and equipment-free technique for AFB1 analysis compared to the traditional analytical methods.

查看原文本刊更多论文

黄曲霉毒素B1 MIP膜智能传感器系统在实际样品应用中的验证

目标校准和验证创新的基于MIP膜的智能手机传感器系统，用于检测真实谷物样品中的黄曲霉毒素B1。方法。根据分子印迹技术，采用原位聚合方法合成了多孔分子印迹聚合物（MIP）膜。使用2-氧代环戊烷羧酸乙酯作为假模板。根据分子动力学的结果，采用丙烯酰胺作为功能单体。使用紫外线聚合30分钟获得60µm厚的多孔MIP膜（Sergeyeva等人，2017）。AFB1与选择性MIP膜表面结合的荧光传感器响应由荧光光谱仪和智能手机相机记录，并由智能手机应用程序Spotxel®Reader（Sicasys Software GmbH，德国）进一步分析。后果获得了基于MIP膜的荧光传感器系统和智能手机传感器系统的校准图。证明了所提出的传感器系统成功应用于实际样品中AFB1分析的可能性。结论。基于MIP膜的智能手机传感器经过校准和验证，能够在真实的谷物样品中可靠、稳健地检测黄曲霉毒素B1。与传统的分析方法相比，所提出的基于MIP膜的智能手机传感器系统为AFB1分析提供了经济实惠、灵敏且无设备的技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.