An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration

Antisense-DNA technologies are new strategy for the treatment of prion infections. This stra-te gy requires prolonged administrations of the drugs, which are likely to alter cell redox processes. Aim. The evaluation of cell survival and intensity of oxidative processes in vitro under the influence of antisense-oligodeoxynucleotides (asODNs) as cell prion inhibitors (PrP C ) complexed with cationic polyelectrolyte. Methods. Free diffusion in agarose gel, study of cytotoxic action on model cells (bull semen), polarography and potentiometric measurement of oxygen uptake, statistical analysis. Results. Poly(dimethylaminoethyl methacrylate)[-]based surfactants form complexes with asODNs. Polyethylene glycol containing surfactants increase oxygen uptake by cells: by 18 % ( VI ), by 37 % ( IV ) and 2.6-fold for V . An addition of the IV -asODNs complex into [the] cell medium did not affect the oxygen absorption; however, it increased reduction processes. Interpolyelectrolyte complex V -asODNs increased the cell respiration by 1.95 times. VI separately increased the cell absorption of oxygen by 18 % and in the complex with asODNs — by 36 %. VI possessed the lowest cytotoxicity. Conclusions. New cationic polyelectrolytes form complexes with asODNs. VI causes the smallest effect on the RedOx processes of model cells and possesses the lowest cytotoxic effect.