Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2021-10-30 DOI:10.7124/bc.000a62

I. Labunets, N. Utko, O. Toporova, S. Savosko, I. Pokholenko, T. Panteleymonova, G. Butenko

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引用次数: 1

Abstract

Aim. To study the influence of melatonin and recombinant human fibroblast growth factor (rhFGF-2) on human umbilical cord multipotent mesenchymal stromal cells (hMMSCs) effects at experimental demyelination. Methods. Adult mice were fed with neurotoxin cuprizone for 3 weeks. hMMSCs (5 × 10 5 cells) were injected on the 10th day of cuprizone diet. Injections of melatonin or rhFGF-2 were started on the 11th day of cuprizone diet. We used cell culture, flow cytometric, spectrophotometric and histological methods, “open field” and “rotarod” tests. Results. Under the cuprizone influence the motor-, emotional activities and muscle tone decreased. The malondialdehyde (MDA) content in brain increased while the activity of antioxidant enzymes decreased. After injection of hMMSCs the number of crossed squares and grooming activity increased while MDA content decreased. Melatonin and rhFGF-2 injections enhanced the effect of cells on grooming activity and increased the glutathione reductase activity. Melatonin also increased the number of boluses, muscle tone and glutathione peroxidase activity. Conclusion. Melatonin and rhFGF-2 improve the effect of hMMSCs in cuprizone-treated mice. The effects of hMMSCs and melatonin combination is greater than that with rhFGF-2.

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褪黑素和成纤维细胞生长因子-2增强人脐带多能间充质基质细胞对铜硫酮诱导的小鼠脱髓鞘的影响

的目标。目的研究褪黑素和重组人成纤维细胞生长因子(rhFGF-2)对实验性人脐带多能间充质基质细胞(hMMSCs)脱髓鞘作用的影响。方法。成年小鼠灌胃神经毒素铜酮3周。第10天注射hMMSCs (5 × 10 5个细胞)。在铜酮饮食的第11天开始注射褪黑激素或rhFGF-2。我们使用了细胞培养、流式细胞术、分光光度法和组织学方法，“开放场”和“旋转体”试验。结果。在铜酮作用下，运动、情绪活动和肌肉张力下降。脑组织丙二醛(MDA)含量升高，抗氧化酶活性降低。注射hMMSCs后，交叉方格数和梳理活性增加，MDA含量降低。褪黑素和rhFGF-2注射增强了细胞对修饰活性的影响，并增加了谷胱甘肽还原酶的活性。褪黑素还增加了丸数、肌肉张力和谷胱甘肽过氧化物酶活性。结论。褪黑素和rhFGF-2可改善铜酮处理小鼠hMMSCs的作用。hMMSCs与褪黑激素联合使用的效果大于与rhFGF-2联合使用。

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.