Microchemical Journal最新文献_第9页

Progress in biosensing applications of isothermal nucleic acid amplification techniques 等温核酸扩增技术在生物传感中的应用进展

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113919

Chaocheng Feng , Yuan Liao , Jinxuan Jia , Lexiang Zhang , Yongxiang Zhao

{"title":"Progress in biosensing applications of isothermal nucleic acid amplification techniques","authors":"Chaocheng Feng , Yuan Liao , Jinxuan Jia , Lexiang Zhang , Yongxiang Zhao","doi":"10.1016/j.microc.2025.113919","DOIUrl":"10.1016/j.microc.2025.113919","url":null,"abstract":"<div><div>Biosensing is an analytical strategy that combines biological recognition elements with physical transduction methods to generate specific quantitative or semi-quantitative analytical information. A major focus in advancing biosensing strategies is the development of efficient signal amplification technologies, which are critical for enhancing detection sensitivity and overall analytical performance. Isothermal amplification technology (IAT) significantly improves biosensing efficiency through rapid and high-yield nucleic acid amplification, and has great potential in on-site, point-of-care and in-situ detection applications. This review aims to summarize recent advancements in various IATs used in biosensing applications. In particular, the mechanism and advantages of IATs in biosensing are introduced, including sensor design principles and the role of signal amplification in improving sensor performance, as well as addressing key technical challenges. Furthermore, the applications and development prospect of integrating IATs and biosensing to realize a variety of small molecules, cancer cells, pathogens, proteins, enzymes as well as nucleic acid analysis are fully discussed, with emphasis on the conversion pathway of the target signal. The primary goal is to highlight the signal amplification capabilities of IATs in biosensing and to provide insight into the design of novel biosensing platforms.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113919"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cu-atom anchoring on covalent organic framework as a wearable flexible electrochemical sensor for acetaminophen 单cu原子锚定共价有机骨架作为对乙酰氨基酚可穿戴柔性电化学传感器

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113923

Zihan Liu , Yao Xu , Yuxin Heng , Fanghua Liu , Chao Tan , Guijiao Wen , Fujian Xu , Juan He , Ke Huang , Xiaoli Xiong , Xue Jiang

{"title":"Single-Cu-atom anchoring on covalent organic framework as a wearable flexible electrochemical sensor for acetaminophen","authors":"Zihan Liu , Yao Xu , Yuxin Heng , Fanghua Liu , Chao Tan , Guijiao Wen , Fujian Xu , Juan He , Ke Huang , Xiaoli Xiong , Xue Jiang","doi":"10.1016/j.microc.2025.113923","DOIUrl":"10.1016/j.microc.2025.113923","url":null,"abstract":"<div><div>In this paper, a wearable flexible patch featuring a non-enzymatic electrochemical sensor based on Cu single-atom catalysts (SACs) anchored on bipyridine-covalent organic framework (COF) electrode was proposed for specific detection of acetaminophen (AP) in human sweat. The electrode modified material is Cu single atom catalytic material (Cu-TpBpy). The bifunctional TpBpy acts as a support for binding individual Cu atoms through abundant and well-organized N-N chelating sites, while simultaneously serving as an active host for specific recognition/adsorption of AP due to its porous structure and active N sites. The resulting Cu-TpBpy with atomically dispersed Cu sites demonstrated high electrochemical activity for AP, attributed to enhanced electron transfer efficiency and reduced binding and adsorption energies. Density functional theory calculations further reveal that the Cu single-atom anchoring on TpBpy provides superior adsorption of AP than TpBpy alone, resulting in lower binding energy, and higher selectivity and sensitivity. The wearable flexible patch, based on Cu-TpBpy, enabled real-time and accurate detection of AP in human sweat and achieved a linear range of 0.3 µM-5 µM and a limit of detection of 13 nM, with satisfied stability and repeatability. This work developed a highly sensitive non-enzymatic sensing method for wearable flexible patches.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113923"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the accuracy of quality assessment for salmon (Salmo salar) by multi-source molecular spectroscopy data fusion and machine learning 基于多源分子光谱数据融合和机器学习的鲑鱼品质评估准确性研究

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113929

Minqiang Guo , Hong Lin , Junlin Chen , Limin Cao , Jianxin Sui , Kaiqiang Wang

{"title":"Improving the accuracy of quality assessment for salmon (Salmo salar) by multi-source molecular spectroscopy data fusion and machine learning","authors":"Minqiang Guo , Hong Lin , Junlin Chen , Limin Cao , Jianxin Sui , Kaiqiang Wang","doi":"10.1016/j.microc.2025.113929","DOIUrl":"10.1016/j.microc.2025.113929","url":null,"abstract":"<div><div>Molecular spectroscopy has emerged as a vital tool for modern food quality monitoring. However, relying on a single spectroscopy technique for non-destructive food quality testing presents limitations in accuracy and comprehensiveness. Here, a novel approach integrating near-infrared (NIR) and Raman spectroscopy data fusion to simultaneously assess salmon (<em>Salmo salar</em>) quality traits under varying storage conditions was developed. By focusing on critical indicators such as Warner-Bratzler shear force (WBSF) and α-helix content, the research aimed to enhance predictive modeling through machine learning, including partial least squares (PLS) and least squares support vector machine (LSSVM), combined with two-dimensional correlation spectroscopy and principal component analysis (PCA). The study revealed significant positive correlations (p < 0.05) between α-helix content and key quality traits, including water-holding capacity (r = 0.66) and WBSF (r = 0.67). WBSF and α-helix were used as potential biomarkers for evaluating the quality of salmon during storage. For predictive modeling, the low-level data fusion LSSVM (LLDF-LSSVM) model demonstrated superior performance in estimating WBSF values (R<sup>2</sup><sub>P</sub> = 0.916, RMSEP = 0.201), outperforming mid-level data fusion (MLDF) and single-spectroscopy models. Additionally, the MLDF-PCA-LSSVM model showed excellent performance in predicting α-helix content (R<sup>2</sup><sub>P</sub> = 0.984, RMSEP = 0.415), enhancing the accuracy of the PCA feature extraction technique for predicting protein secondary structure. By bridging spectroscopy and advanced machine learning, the findings offer novel insights into real-time quality monitoring, supporting enhanced food safety protocols and reducing waste in the seafood supply chain.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113929"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A first in class disposable copolymer-graphite platform for electrocatalytic detection of dipicrylamine explosive in wastewater and soil samples 一种用于电催化检测废水和土壤样品中二苯胺炸药的一次性共聚物-石墨平台

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113930

Ecenur Daşdemir , Nazife Aslan , Nilgün Şen , Lokman Liv

{"title":"A first in class disposable copolymer-graphite platform for electrocatalytic detection of dipicrylamine explosive in wastewater and soil samples","authors":"Ecenur Daşdemir , Nazife Aslan , Nilgün Şen , Lokman Liv","doi":"10.1016/j.microc.2025.113930","DOIUrl":"10.1016/j.microc.2025.113930","url":null,"abstract":"<div><div>A newly developed, cost-effective, easy-to-prepare, disposable, highly selective, and sensitive electrode was successfully created by simultaneously electropolymerizing tartrazine and ponceau monomers on graphite. This electrode was effectively used to detect the hazardous explosive dipicrylamine in real samples. The fabricated sensor was thoroughly characterized using CV, SEM, EDX, and XPS techniques. It demonstrated a significant calibration sensitivity of <span><math><mrow><mn>53.23</mn><mspace></mspace><mi>μ</mi><mi>A</mi><mo>.</mo><msup><mrow><mi>L</mi><mo>.</mo><mi>m</mi><mi>g</mi></mrow><mrow><mo>-</mo><mn>1</mn></mrow></msup><mo>.</mo><msup><mrow><mi>c</mi><mi>m</mi></mrow><mrow><mo>-</mo><mn>2</mn></mrow></msup></mrow></math></span>, a low limit of detection of 48.7 μg/L (visually 25 μg/L) and broad analytical ranges of 0.16–1.00, 2–10, and 14–50 mg/L. Furthermore, the sensor exhibited noteworthy repeatability (<3.8%), reproducibility (<2.3%), and stability, with a maximum 6.7% signal change after 7 days. To assess accuracy, the method was verificated using certified reference materials, including Elements in Soil (UME EnvCRM 03) and Elements in Wastewater (UME CRM 1204). Additionally, the results of the developed method were statistically compared with HPLC analyses using the CRMs.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113930"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of molecularly imprinted polymer encapsulated with quantum dots for detection of crocin as an alternative approach for quality estimation of saffron 用量子点包封分子印迹聚合物检测藏红花素作为藏红花质量评价的替代方法

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113937

Tahir ul Gani Mir , Saurabh Shukla , Jaskaran Singh , Amel Gacem , Suhas Ballal , Krishna Kumar Yadav , Lamjed Mansour , Abhinav Kumar , Mudasir A. Dar , Atif Khurshid Wani

{"title":"Synthesis of molecularly imprinted polymer encapsulated with quantum dots for detection of crocin as an alternative approach for quality estimation of saffron","authors":"Tahir ul Gani Mir , Saurabh Shukla , Jaskaran Singh , Amel Gacem , Suhas Ballal , Krishna Kumar Yadav , Lamjed Mansour , Abhinav Kumar , Mudasir A. Dar , Atif Khurshid Wani","doi":"10.1016/j.microc.2025.113937","DOIUrl":"10.1016/j.microc.2025.113937","url":null,"abstract":"<div><div>Molecularly Imprinted Polymers (MIPs) are widely used for their selective recognition capabilities, synthesized through interactions between template molecules, functional monomers, crosslinkers, and solvents. In this study, MIPs encapsulated with Carbon Quantum Dots (CQDs) were synthesized for detection of crocin, a bioactive carotenoid responsible for saffron’s vivid colour. CQDs were synthesized via thermal pyrolysis and characterized using TEM, FTIR, XRD, XPS, and fluorescence spectroscopy. The MIP-CQDs nanocomposite was prepared by integrating CQDs with functional monomers and crosslinkers, followed by template removal. Comparative non-imprinted polymers (NIPs) were also synthesized. The nanocomposites exhibited excellent optical and morphological properties, with fluorescence quenching used to evaluate crocin interaction. The MIP-CQDs displayed high sensitivity and selectivity for crocin in a linear range of 2–175 μM, with a detection limit of 2.1 μM. Optimal conditions included pH 7.0 and a 5-minute incubation time. The fluorescence quenching mechanism was attributed to Forster Resonance Energy Transfer (FRET) between crocin and MIP-CQDs. Notably, MIP-CQDs showed strong resistance to interference from structurally similar compounds and co-existing ions, ensuring reliable performance in complex matrices. The study underscores the significance of tailored nanocomposite designs for advancing molecular sensing applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113937"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted fluorescence sensor based on Fe3O4/CDs@MIPs for detecting the gastric cancer marker p-Hydroxybenzoic acid 基于Fe3O4/CDs@MIPs的分子印迹荧光传感器检测胃癌标志物对羟基苯甲酸

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113935

Rui Jie , Xiaochen Zhu , Haiyan Qi , Tao Jing , Jun Li , Yunpeng Zhao , Jinzhi Yu , Chenyu Qi

{"title":"Molecularly imprinted fluorescence sensor based on Fe3O4/CDs@MIPs for detecting the gastric cancer marker p-Hydroxybenzoic acid","authors":"Rui Jie , Xiaochen Zhu , Haiyan Qi , Tao Jing , Jun Li , Yunpeng Zhao , Jinzhi Yu , Chenyu Qi","doi":"10.1016/j.microc.2025.113935","DOIUrl":"10.1016/j.microc.2025.113935","url":null,"abstract":"<div><div>With the rapid development of metabolomics, p-Hydroxybenzoic acid in urine has been identified as a crucial biomarker for the early detection and screening of gastric cancer. In this study, we designed and synthesized a novel magnetic hollow carbon dot molecularly imprinted fluorescence sensor (Fe<sub>3</sub>O<sub>4</sub>/CDs@MIPs) to achieve high sensitivity and selectivity detection of this biomarker. The incorporation of Fe<sub>3</sub>O<sub>4</sub> nanoparticles into the sensor significantly improved its recognition capability and analytical performance. The fluorescence sensor exhibited a strong linear response within the concentration range of 0–100 μmol/L, with an impressive detection limit of 0.37 μmol/L. The sensor demonstrated excellent specificity, showing minimal interference from common cations, anions, amino acids, and structural analogs, underscoring its high selectivity for p-Hydroxybenzoic acid. In practical applications, spike recovery experiments conducted on urine samples from both gastric cancer patients and healthy individuals revealed recovery rates ranging from 95.00 % to 106.67 % and relative standard deviations between 0.76 % and 2.80 %. These results confirmed the sensor’s outstanding accuracy, reproducibility, and stability. Overall, the Fe<sub>3</sub>O<sub>4</sub>/CDs@MIPs sensor provides a robust, non-invasive instrument for detecting gastric cancer biomarkers in urine, offering significant potential for early diagnosis and monitoring of gastric cancer.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113935"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated data exploration of α-pyrrolidinoisohexiophenone metabolites in Upcyte human hepatocytes reveals potential individual-specific metabolic profiles upcell人肝细胞α-吡咯烷二异己烯酮代谢物的自动数据探索揭示了潜在的个体特异性代谢谱

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113908

Francesc A. Esteve-Turrillas , Alicia Trigueros-Sancho , Ramiro Jover , David Pérez-Guaita , Guillermo Quintás

{"title":"Automated data exploration of α-pyrrolidinoisohexiophenone metabolites in Upcyte human hepatocytes reveals potential individual-specific metabolic profiles","authors":"Francesc A. Esteve-Turrillas , Alicia Trigueros-Sancho , Ramiro Jover , David Pérez-Guaita , Guillermo Quintás","doi":"10.1016/j.microc.2025.113908","DOIUrl":"10.1016/j.microc.2025.113908","url":null,"abstract":"<div><div>Synthetic cathinones, a major class of new psychoactive substances, pose significant public health challenges due to their toxicity and frequent involvement in overdoses. However, detecting them in biological samples remains challenging because of their structural similarities and a lack of metabolic data. This study analyses the <em>in vitro</em> toxicity and metabolism of the synthetic cathinone α-pyrrolidinoisohexiophenone (α-PiHP) using Upcyte Human Hepatocytes (UHH) as hepatic cell model. Liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-HRMS/MS) was employed to analyze both cell media and intracellular contents. An automatic data mining strategy was developed to identify putative metabolites based on predicted biotransformations, MS/MS spectral similarities among putative metabolites and the parent compound, and unique signals in treated samples. This workflow led to the identification of 10 Phase I metabolites. The most abundant metabolite in cell extracts was M1 (C<sub>16</sub>H<sub>25</sub>NO, α-PiHP ketone β-reduction), while metabolite M8 (C<sub>16</sub>H<sub>23</sub>NO<sub>3</sub>, α-PiHP dihydroxylation) predominated in the cell medium, suggesting their potential as biomarkers for α-PiHP consumption. Notably, interindividual differences in <em>in vitro</em> hepatic metabolism were observed across two donors, indicating idiosyncratic metabolic profiles, and underscoring the importance of human variability in drug metabolism studies and in forensic and clinical applications. In summary, these findings enhance our understanding of α-PiHP metabolism and may improve the detection of synthetic cathinones in biological and environmental samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113908"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Zr/polyoxometalate/carbon nanotube ternary composite for the efficient dispersive micro solid-phase extraction of phenolic derivatives Zr/多金属氧酸盐/碳纳米管三元复合材料高效分散微固相萃取酚类衍生物的研制

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113922

Mojtaba Sohbati-barghanabadi, Maryam Bazargan, Masoud Mirzaei, Amirhassan Amiri

{"title":"Development of Zr/polyoxometalate/carbon nanotube ternary composite for the efficient dispersive micro solid-phase extraction of phenolic derivatives","authors":"Mojtaba Sohbati-barghanabadi, Maryam Bazargan, Masoud Mirzaei, Amirhassan Amiri","doi":"10.1016/j.microc.2025.113922","DOIUrl":"10.1016/j.microc.2025.113922","url":null,"abstract":"<div><div>In this work, a tri-component composite containing zirconium nanoparticles/polyoxometalate/carbon nanotube (ZrNP@SiW<sub>12</sub>-CNT) was developed as sorbent for the dispersive micro solid-phase extraction (D-μSPE) of phenolic derivatives (i.e. phenol, 2-chlorophenol, 3-chlorophenol, and 4-nitrophenol) from water samples. The structure and morphology of the sorbent were studied by different techniques in which the Zr nanoparticles were chosen to be encapsulated by Keggin-type polyoxometalate [SiW<sub>12</sub>O<sub>40</sub>]<sup>4−</sup> (SiW<sub>12</sub>) units to form ZrNP@SiW<sub>12</sub> clusters. Then, these clusters are assembled on the surface of oxidized carbon nanotube (CNT) by many types of electrostatic interactions to form ZrNP@SiW<sub>12</sub>-CNT composites. Therefore, ZrNP@SiW<sub>12</sub>-CNT with aromatic and oxygen-rich surface (oxo-groups from polyoxometalate (POM) clusters, carboxylate, and hydroxy groups of oxidized CNT) can be a suitable sorbent to anchor phenolic pollutants (containing aromatic ring, hydroxyl group, and halogen or nitro groups) via electrostatic interactions like π-stacking, hydrogen and halogen bonding. The extracted phenolic derivatives were analyzed using a gas chromatography-flame ionization detector (GC-FID) with linear calibration plots in the concentration range of 0.03–200 ng mL<sup>−1</sup>. The limits of detection (LODs) were determined as 0.01–0.02 ng mL<sup>−1</sup> which showed that the D-μSPE-GC-FID method is valid for the extraction of phenolic derivatives from water samples with relative recovery values of 96.6–99.9 %. Also, the relative standard deviation (RSD) range was 3.6–4.7 %.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113922"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive colorimetric and ratio l-arginine probe for smartphone detection and living roe imaging 灵敏比色法和比例l-精氨酸探针用于智能手机检测和活卵成像

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113936

Yanli Leng , Qiao Li , Ye Wang , Yongqin Liu , Fanghui Tao , Xiaohua Cai , Xiaohong Li , Xuemei Chen , Jungang Wang , Jianhui Zhang

{"title":"Sensitive colorimetric and ratio l-arginine probe for smartphone detection and living roe imaging","authors":"Yanli Leng , Qiao Li , Ye Wang , Yongqin Liu , Fanghui Tao , Xiaohua Cai , Xiaohong Li , Xuemei Chen , Jungang Wang , Jianhui Zhang","doi":"10.1016/j.microc.2025.113936","DOIUrl":"10.1016/j.microc.2025.113936","url":null,"abstract":"<div><div>Owing to the importance of <em>L</em>-Arginine (Arg) in humans and organisms, it is necessary to develop efficient methods to detect it. In this article, a novel colorimetric and ratio Arg probe <strong>L1</strong> was developed via a simple synthetic process from economical substrates. The D-π-A structure having a large conjugated system endowed it with the high performance of large Stokes shift and obvious color changes both in its UV and fluorescence. The dual-signal sensor was constructed with excellent colorimetric sensitivity with the detection limit (<em>LOD</em>, S/N = 3) of 57.91 nM, which can realize the detection of Arg for qualitative analysis without any instrument. Besides, the probe also has relatively great selectivity and fluorescence sensitivity with the detection limit of 131.5 nM. Meanwhile, the short response time within 2 min allowed it to detect Arg immediately. The binding mechanism was also investigated by various ways, job’s plots indicated a <strong>2:1</strong> stoichiometric ratio of <strong>L1</strong> with Arg, and the synergistic effects of deprotonation and intermolecular hydrogen bonding are considered as the primary interactions through <sup>1</sup>H NMR titration experiments and theoretical calculation. The frontier orbitals distribution and their respective energies of <strong>L1</strong> and 2<strong>L1</strong> + Arg complex were obtained too, the reduced energy gap corresponded with the red shift of the absorption peak. Based on these excellent recognition behavior, probe <strong>L1</strong> was not only used as the convenient Arg detection sensor in various food by a smartphone, but also applied in the living fertilized medaka egg imaging.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113936"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleic acid aptamer-based biosensor for health monitoring: A review and future prospects 基于核酸适体的健康监测生物传感器研究进展及展望

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-05-10 DOI: 10.1016/j.microc.2025.113894

Wenji Bao , Gerile Aodeng , Lu Ga , Jun Ai

{"title":"Nucleic acid aptamer-based biosensor for health monitoring: A review and future prospects","authors":"Wenji Bao , Gerile Aodeng , Lu Ga , Jun Ai","doi":"10.1016/j.microc.2025.113894","DOIUrl":"10.1016/j.microc.2025.113894","url":null,"abstract":"<div><div>Functional nucleic acids, beyond their role as carriers of genetic information, exhibit a unique ability to bind specifically to biomarkers. Their notable advantages, including ease of synthesis, flexible labeling, high stability, and cost-effectiveness, have enabled their broad applications in diverse fields such as biomedicine, food safety, and environmental monitoring. In health monitoring, biomarkers found in biological samples—such as blood, sweat, urine, or soft tissues—can be analyzed to evaluate an individual’s health status. Biosensors, typically composed of a molecular recognition element and a signal transducer, enable such detection with high efficiency. Among them, those utilizing nucleic acid aptamers as recognition probes offer a versatile platform for identifying various biomarkers with excellent specificity and sensitivity. This review provides a comprehensive summary of the signal transduction mechanisms underlying aptamer-based sensors and highlights recent advances in their applications for health monitoring. Notably, health-related detection extends beyond disease diagnosis to include monitoring of foodborne and environmental factors that impact well-being. By integrating signal amplification strategies, numerous highly sensitive and selective biosensing techniques have been developed for detecting targets such as proteins, organic molecules, viruses, heavy metal ions, and bacteria, with significant implications for the medical and food safety domains. These advancements pave the way for novel applications of nucleic acid aptamers in chemistry, materials science, medicine, and environmental research, further expanding their potential in interdisciplinary fields.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"214 ","pages":"Article 113894"},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0