Microchemical Journal最新文献

{"title":"Potential of high-throughput FIA-MS/MS and LC-MS/MS polyphenolic profiling to assess tea authenticity. Application to tea adulterations with chicory","authors":"Thom Romers ,&nbsp;Sònia Sentellas ,&nbsp;Javier Saurina ,&nbsp;Oscar Núñez","doi":"10.1016/j.microc.2024.111723","DOIUrl":"10.1016/j.microc.2024.111723","url":null,"abstract":"<div><div>Tea can be found among the beverages more susceptible to fraudulent practices because of its high worldwide consumption and the increases on prices for some specific varieties due to climate change and geopolitical instability. Tea adulteration with other plants, such as chicory, is a common practice to gain an illicit profit. Polyphenols are abundant bioactive substances in tea, determining its quality and health function. In addition, they can be employed as markers to address authentication issues. The present contribution assesses the potential of polyphenolic profiling by high-throughput FIA-MS/MS and LC-MS/MS methodologies for tea authenticity. One hundred tea samples belonging to different varieties (green, black, red, oolong, and white teas) and 20 chicory samples were analyzed with both methodologies after a simple brewing process to profile fifty-five polyphenols belonging to different families. The resulting chemical descriptors were used to address tea classification and authentication by partial least-squares-discriminant analysis (PLS-DA). An excellent classification performance by PLS-DA was accomplished, with sensitivity and specificity values for FIA-MS/MS higher than 90% and 88.9%, respectively, and for LC-MS/MS higher than 85% and 86%, respectively. Good accuracy was also attained, with calibration errors below 10.5 and 14.5% for FIA-MS/MS and LC-MS/MS, respectively. Overall, FIA-MS/MS showed a better performance than LC-MS/MS, with the additional advantage of shorter analysis time as no chromatographic separation was required. The capability of phenolics to quantify tea adulterations with chicory was also assessed by partial least squares (PLS) regression, with prediction errors below 10.9 and 14.8% for FIA-MS/MS and LC-MS/MS, respectively, in the determination of adulterant levels. Thus, both methodologies demonstrated to be feasible for assessing tea authentication issues.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111723"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0026265X24018356/pdfft?md5=7fec1c6224a25afd2c9751d5ce03ded3&pid=1-s2.0-S0026265X24018356-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-pot synthesis of polypyrrole‐coated rubber foam for the extraction of carbendazim residue in orange juice","authors":"Teerayanee Chaipet ,&nbsp;Suwatchanee Maneeratanachot ,&nbsp;Nussana Lehman ,&nbsp;Ekwipoo Kalkornsurapranee ,&nbsp;Wing Cheung Mak ,&nbsp;Proespichaya Kanatharana ,&nbsp;Panote Thavarungkul ,&nbsp;Chongdee Thammakhet-Buranachai","doi":"10.1016/j.microc.2024.111712","DOIUrl":"10.1016/j.microc.2024.111712","url":null,"abstract":"<div><div>Natural rubber foam was coated with polypyrrole (PPy) via a rapid two-pot synthesis. This PPy-coated rubber foam was used as sorbent for the solid-phase extraction of carbendazim in fresh orange juice. The extracted carbendazim was quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). Under the optimal conditions, the method exhibited linearity in the range of 25–1000 µg kg⁻¹ with a limit of detection (LOD) of 24.1 µg kg⁻¹ and a limit of quantification (LOQ) of 80.5 µg kg⁻¹. Good reproducibility (RSD &lt; 2.5 %, n = 6) and good reusability (RSD &lt; 3.8 %, n = 10) were achieved. The sorbent was applied to extract carbendazim in fresh orange juice. Accuracy was excellent and recoveries were attained from 81.6 % to 104.8 %. The found carbendazim in real samples ranged from 40.0 µg kg⁻¹ to 101.1 µg kg⁻¹. The developed sorbent can be produced in large quantities at a reasonable cost and utilized as an environmentally friendly sorbent for the extraction of pesticides and other trace organic substances.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111712"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of energy modality on tissue identification from surgical smoke by differential ion mobility spectrometry","authors":"Patrik Sioris ,&nbsp;Meri Mäkelä ,&nbsp;Anton Kontunen ,&nbsp;Markus Karjalainen ,&nbsp;Antti Vehkaoja ,&nbsp;Niku Oksala ,&nbsp;Antti Roine","doi":"10.1016/j.microc.2024.111733","DOIUrl":"10.1016/j.microc.2024.111733","url":null,"abstract":"<div><div>Surgical smoke analysis offers a way to provide assisting information to the surgeon intraoperatively, which can be potentially used to assess cancer tumor margins in surgical oncology and alert the operator of accidental organ injuries caused by electrosurgical (ES) instruments. Surgical smoke content is affected by the energy instrument it is produced by. Classification of surgical smoke by differential ion mobility spectrometry (DMS) was evaluated with 6 porcine tissue types and 5 energy instruments. Instruments consisted of mono- and bipolar instruments, ultrasonic shears and a –blade. Machine learning was used to classify tissues by training binary classifier linear discriminant analysis (LDA) to distinguish a marked tissue class from the rest. The greatest binary classification accuracies were obtained with the monopolar instruments and the lowest with the bipolar instrument, 93.5 % and 77.5 % respectively. The analysis of surgical smoke with DMS is possible with a variety of energy instruments, however with varying performance. This implies that DMS based tissue identification is generalizable across different surgical instruments and surgical specialties.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111733"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0026265X24018459/pdfft?md5=7b047d8b0257ec6cbded8f7d55647ee7&pid=1-s2.0-S0026265X24018459-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boronate affinity molecularly imprinted electrochemical sensor based on electrospun carbon nanofibres for selective kanamycin detection","authors":"Jixiang Liu ,&nbsp;Yukun Xing ,&nbsp;Chengkai Lv,&nbsp;Lan Luo,&nbsp;Fangfang Chen","doi":"10.1016/j.microc.2024.111743","DOIUrl":"10.1016/j.microc.2024.111743","url":null,"abstract":"<div><div>The presence of trace kanamycin (KANA) residues in animal-derived foods from its improper use present a critical public health issue, making the urgent need for the development of sensitive and selective KANA detection methods. In this study, we developed molecularly imprinted polymers (MIPs)-based electrochemical sensor for the detection of KANA, incorporating Co, Mo-doped carbon nanofibres (Co, Mo@CNFs) to modify a glassy carbon electrode (GCE). Then, the MIPs layer was synthesized via electropolymerization using KANA as a template molecule and 3-aminophenylboronic acid (3-APBA) as the functional monomer (as MIPs/Co, Mo@CNFs/GCE), facilitating selective recognition through boric acid affinity. The presence of Co, Mo@CNFs enhanced electron transport across the otherwise insulating MIPs layer, thereby improving the sensor’s selective recognition capabilities. Under optimal conditions, the MIPs/Co, Mo@CNFs/GCE sensor demonstrated precise KANA detection within a concentration range of 1 × 10⁻² to 1 × 10⁵ nmol·L⁻¹, achieving a detection limit of 2.56 pmol·L⁻¹. Furthermore, the sensor’s applicability was validated in complex matrices, successfully detecting KANA in serum and milk samples with recovery rates ranging from 95.05 % to 116.43 %. These results confirmed that the proposed sensor is highly effective for detecting KANA in both biological and animal-origin food samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111743"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA tetrahedral scaffold based one-step ratiometric electrochemical detection of aflatoxin B1 in tobacco samples","authors":"Fan He ,&nbsp;Shaohe Jiao ,&nbsp;Rongxing Zou ,&nbsp;Miao Lai ,&nbsp;Mingqin Zhao ,&nbsp;Nianlei Wang ,&nbsp;Hong Zhang","doi":"10.1016/j.microc.2024.111746","DOIUrl":"10.1016/j.microc.2024.111746","url":null,"abstract":"<div><div>To effectively monitor Aflatoxin B1 (AFB₁) in tobacco samples, a ratiometric electrochemical biosensor based on DNA tetrahedral scaffold (DTS) and AuNPs/chitosan (CS) modified electrode has been successfully fabricated. The ultra-thin AuNPs not only present a larger specific sensing surface, but also can graft more DTS onto the surface of the electrode. The DTS is directly grated with two strands of AFB₁ aptamer, which leading to a facile one-step detection of AFB₁. In the presence of AFB₁, the current of methylene blue (MB) keeps stable, while the current of ferrocene (Fc) decreases since the target takes away the Fc-labeled aptamer from the electrode surface. The fabricated biosensor can monitor AFB₁ in the linear range of 0.01–1000 pg·mL⁻¹ with the detection limit of (LOD) 2.6 fg·mL⁻¹. More importantly, the biosensor performs well in the detection of AFB1 in tobacco samples with the LOD of 2.86 fg·mL⁻¹. The developed ratiometric electrochemical biosensor displays high selectivity, good stability and high potential in tobacco sample’s AFB₁ detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111746"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the detection of breast cancer by nucleic acid aptasensor","authors":"Yue Yang ,&nbsp;Lingli Qu ,&nbsp;Zekai Wang ,&nbsp;Li Tang ,&nbsp;Siyue Tian ,&nbsp;Yiyang Zhou ,&nbsp;XuYi wen ,&nbsp;Hongzhi Pan","doi":"10.1016/j.microc.2024.111699","DOIUrl":"10.1016/j.microc.2024.111699","url":null,"abstract":"<div><div>Breast cancer is the most prevalent malignant tumor among women globally. Early screening for breast cancer significantly enhances treatment outcomes. Biosensors offer high sensitivity, simple operation, low equipment costs, and the capability to quantitatively detect diverse tumor markers. Nucleic acid aptamer sensors are composed of single stranded oligonucleotides containing DNA or RNA, which can be combined with various nanomaterials to produce more sensitive and reliable biosensor platforms. This adapter sensor has been successfully applied in sensor methods such as electrochemistry, fluorescence, surface-enhanced raman spectroscopy, field effect transistors, microfluidic chips, paper-based sensing, etc., with significant specificity, stability, and good biocompatibility. Nucleic acid aptamer sensors are highly favored due to their sensitivity, responsiveness, and selectivity. This review mainly discusses the nucleic acid aptamer sensors using various sensitivity enhancement techniques and nanomaterials to detect the biomarkers, and provides a brief overview of their application prospects.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111699"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PANI-functionalized MXene/SnOx porous heterostructures as a novel electrochemical sensing platform for detecting luteolin in food","authors":"Ran Liao ,&nbsp;Yu Zhong ,&nbsp;Ling Deng ,&nbsp;Guowen He ,&nbsp;Saiwen Liu ,&nbsp;Jin Zhang ,&nbsp;Chao Chen","doi":"10.1016/j.microc.2024.111711","DOIUrl":"10.1016/j.microc.2024.111711","url":null,"abstract":"<div><div>Luteolin is a natural flavonoid compound widely found in fruits, vegetables, and herbs. Reasonable intake is beneficial for the treatment of cough, inflammation, cardiovascular disease and other diseases. In this study, MXene/SnO_x composites were first prepared hydrothermally and then functionalized by in situ chemical polymerization to form MXene/ SnO_x/PANI composites with three-dimensional heterogeneous conductive structures. The composites were characterized via SEM, XRD, and XPS. Compared with MXene, the addition of SnO_x results in composites with a larger specific surface area and more exposed active sites. In addition, the in situ polymerization and functionalization of PANI on the surface of MXene/SnO_x promotes an increase in the number of electronic conduction paths in the composite structure and effectively accelerates charge transfer at the electrode interface. The formation of a three-dimensional heterogeneous conductive structure promotes the electrochemical redox process of Lut at the sensing interface. On the basis of these excellent properties, the MXene/SnO_x/PANI modified electrode has outstanding performance in Lut detection, with a detection range of 0.2–6.5 μM and a detection limit of 0.12 μM. In addition, it has excellent repeatability, stability, and anti-interference ability and can be used to accurately determine the Lut content in real Perilla and Chrysanthemum samples, and the results are consistent with those of UV–Vis.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111711"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel enzyme cascade colorimetric nanosensing platform based on 3D diatomic nanozymes synergistically enhancing peroxidase-like activity for the detection of xanthine","authors":"Zichen Song ,&nbsp;Jiayao Ou ,&nbsp;Fengxiao Zhu ,&nbsp;Yifan Wang ,&nbsp;Hongda Zhu ,&nbsp;Huiling Guo ,&nbsp;Hongmei Sun ,&nbsp;Mingxing Liu","doi":"10.1016/j.microc.2024.111735","DOIUrl":"10.1016/j.microc.2024.111735","url":null,"abstract":"<div><div>Abnormal xanthine (XA) levels could cause various diseases, making sensitive and reliable XA detection crucial for early diagnosis. In this study, novel enzyme cascade colorimetric nanosensing platform based on 3D PtAu/PCN/S-rGO diatomic nanozymes with peroxidase-like (POD-like) activity was designed and fabricated. The doping of S and P increased the electron transfer rate, and the synergy of 3D PCN/S-rGO carriers increased the POD-like activity of diatomic nanozymes. Additionally, an efficient enzyme cascade nanosensing platform XOD@3D PtAu/PCN/S-rGO for the colorimetric detection of XA was constructed by utilizing this nanocomposite as a carrier for immobilizing xanthine oxidase (XOD) which could effectively enhance cascade efficiency. The intermediate •OH was produced during the cascade reaction, and the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by •OH was catalyzed by the nanocomposites to form blue oxTMB, which increased the absorbance at 652 nm and thus generated a colorimetric signal for the detection of XA. The linear range of XA detection was 1.5–250 μM, and the limit of detection was 1.29 μM. This nanosensing platform exhibited significant reproducibility, specificity, and stability, making it suitable for detecting XA in real human blood samples. Therefore, this enzyme cascade colorimetric diatomic nanosensing platform has potential applications for the detection of XA and other biomarkers in actual clinical samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111735"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptasensing of rivaroxaban in human plasma using KCC-1-NH-CS2 modified conductive nano-ink: A new biosensor","authors":"Rokhsareh Ebrahimi ,&nbsp;Mohammad Hasanzadeh ,&nbsp;Nasrin Shadjou ,&nbsp;Azadeh Nilghazi","doi":"10.1016/j.microc.2024.111744","DOIUrl":"10.1016/j.microc.2024.111744","url":null,"abstract":"<div><div>An innovative paper-based electrochemical aptasensor has been developed for the detection of Rivaroxaban (RIV) in human plasma samples. The sensor is fabricated using a novel prob by encapsulating aptamer-aminated on dendritic fibrous nano-silica (KCC-1-NH-CS₂) particles. The prob is then immobilized on the functionalized silver nano-ink electrode.. The resulting redox reaction on the prob was subsequently evaluated using square wave voltammetry, differential pulse voltammetry, and chronoamperometry. Under optimized condition and using a label-free strategy, the aptasensor exhibited suitable sensitivity and specificity for detecting RIV with a linear range of 10 to 1000 nM and a lower limit of quantification (LLOQ) of 10 nM. The engineered aptasensing platform shows great potential for the fabrication of other cost-effective, sensitive, and portable biosensors for the pharmaceutical and biomedical analysis.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111744"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered nanozyme-cascade catalyzed reaction for rapid acid phosphatase detection","authors":"Nannan Li ,&nbsp;Fan Yang ,&nbsp;Liping Li ,&nbsp;Ruiping Zhang","doi":"10.1016/j.microc.2024.111688","DOIUrl":"10.1016/j.microc.2024.111688","url":null,"abstract":"<div><div>The utilization of artificial multienzyme-catalyzed cascade reactions have greatly benefited biosensors. This study presents a novel nanoreactor consisting of manganese ions-doped hollow carbon nanospheres (Mn@HCNs), which can rapidly transform molecular information into an easily comprehensible colorimetric signal. The Mn@HCNs nanoreactor was utilized to detect acid phosphatase (ACP) within a range of 0.5–10 mU/mL, achieving a limit of detection (LOD) as low as 0.0851 mU/mL. The superiority of our nanoreactor over traditional ACP detection methods stems from its exceptional substrate affinity and the multi-enzymatic behaviors of Mn@HCNs. Especially, the specific POD-like activity (SA) value of Mn@HCNs was superior to most reported Fe₃O₄ and other single-atom enzymes. Furthermore, this nanoreactor exhibited exceptional selectivity against protein and ion interference, and was furthered to detect ACP in clinical serum samples. The work may extend the development of artificial multienzyme-catalyzed cascade reactions for molecular biosensors and clinical application.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111688"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}