中国血吸虫病防治杂志最新文献

{"title":"[Investigation of tick species in Suizhou City, Hubei Province from 2023 to 2024].","authors":"H Lu, F Guo, Y Pan, M Peng, L Wu, Y Lin, X Liu, X Yu","doi":"10.16250/j.32.1915.2024185","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024185","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the species of ticks in Suizhou City, Hubei Province, so as to provide insights into management of ticks and tick-borne diseases.</p><p><strong>Methods: </strong>During the period between May 2023 and June 2024, livestock breeding farms and vegetation neighboring the place of residence of confirmed and suspected patients with tick-borne disease were selected as sampling points in rural areas from Yindian Township, Gaocheng Township, Wanhe Township, Wushan Township, Xiaolin Township, Xihe Township, Hedian Township and Beijiao Street in Suizhou City, Hubei Province, where confirmed and suspected cases with tick-borne diseases had been reported. The parasitic ticks on the body surface of free-range livestock were captured with tweezers in livestock breeding farms, and free ticks on the vegetation surface were captured with the flagging method. Morphological identification of tick samples was performed under a microscope, and the gender and developmental stage of ticks were determined. One engorged adult tick, 2 to 3 blood-feeding but non-engorged adult ticks, 10 to 15 unfed female ticks, 15 to 20 unfed male ticks, and 30 to 40 tick nymphs or larvae were assigned into a group, respectively. Genomic DNA was extracted from tick samples in each group, and mitochondrial <i>16S rRNA</i> gene was amplified. Sequence analysis was performed with the DNASTAR software, and phylogenetic analysis was performed using the software MEGA 7.0. In addition, the phylogenetic tree was generated using the maximum likelihood method based on the Kimura 2 parameter model.</p><p><strong>Results: </strong>A total of 2 438 ticks were captured from Suizhou City, Hubei Province during the period between May 2023 and June 2024, including 595 free ticks and 1 483 parasitic ticks. Three developmental stages of ticks were captured, including larvae, nymphs, and adults, and 75.18% (1 899/2 438) of captured ticks were adult, in which 79.04% (1 501/1 899) were female. Morphological and molecular biological assays identified one family, three genera and four species of captured ticks, including 2 425 <i>Haemaphysalis longicornis</i> ticks (99.47%) and one <i>H. flava</i> tick (0.04%) of the genus <i>Haemaphysalis</i>, 11 <i>Rhipicephalus microplus</i> ticks (0.45%) of the genus <i>Rhipicephalus</i>, and one <i>Ixodes sinensis</i> tick (0.04%) of the genus <i>Ixodes</i> in the family Ixodidae. Phylogenetic analysis revealed that the <i>H. longicornis</i> sequence (SZ49) in this study was clustered with sequences from Yunnan Province (GenBank accession number: MH024510.1), Hebei Province (GenBank accession number: MK450606.1) and Henan Province (GenBank accession number: MZ230645.1) into a clade, and the <i>H. flava</i> sequence (SZ19) in this study was clustered with sequences from Japan (GenBank accession number: MW064044.1), South Korea (GenBank accession number: ON629585.1), and Jiangsu Province (GenBank accession number: PP494741.1) and H","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"184-189"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Strategic thinking on management of cross-boundary imported schistosomiasis].","authors":"J Xu, S Li, Q Li, S Guo, S Li, X Zhou","doi":"10.16250/j.32.1915.2025018","DOIUrl":"https://doi.org/10.16250/j.32.1915.2025018","url":null,"abstract":"<p><p>Schistosomiasis is prevalent in 78 countries and territories worldwide, while the eastern and western parts of sub-Sahara Africa bear the highest disease burden due to schistosomiasis. Recently, climate change, international trade and travel, urbanization and war have increased the risk of cross-boundary importation and transmission of schistosomiasis, and schistosomiasis has increasingly become a public health concern in non-endemic countries and territories. Biomphalaria straminea, the intermediate host of Schistosoma mansoni, has colonized in southern China and its habitats continue to move northward. In addition, cross-boundary imported cases of schistosomiasis have been reported occasionally in China. However, the real number of cases may be underestimated greatly due to insufficient diagnostic capacity and weak awareness of case reporting for overseas imported schistosomiasis in healthcare facilities. It is necessary to establish a multi-party collaborative mechanism, improve corresponding systems and technical specifications, reinforce surveillance and early warning, and border management, enhance technical reserves and capability building, and improve the awareness of schistosomiasis prevention and healthcare-seeking among entry-exit personnel, in order to effectively address the threat of cross-boundary imported schistosomiasis.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"107-111"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Current status of anticoccidial drug resistance in China].","authors":"Y Bai, W Li, W Luo, Y Yu, D Li, J Zhao, L He","doi":"10.16250/j.32.1915.2024147","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024147","url":null,"abstract":"<p><p>Avian coccidiosis, an acute parasitic disease that mainly harms chicks, is widely prevalent across the world, which poses a serious threat to poultry industry. Because of the single prophylactic formulations, veterinary clinical treatment of coccidiosis mainly relies on chemically synthesized agents, polyether ionophores and Chinese herbal medicines. The introduction of novel anticoccidial drugs is slow for a long period of time, and there is an increasing problem of anticoccidial drug resistance following long-term use, which has become an urgent problem to be solved in poultry industry. This review summarizes the levels of anticoccidial drug resistance across China from 2018 to 2023, and analyzes the resistance to various anticoccidial agents in coccidia. It is indicated that the overall prevalence of anticoccidial drug resistance is high in coccidia, and development of novel anticoccidial agents and products with reduced antibiotics use and alternatives of antibiotics is of an urgent need.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"217-222"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effectiveness of the integrated schistosomiasis control programme in Wuhan City from 2005 to 2023].","authors":"S Wang, H Luo, Y Li, H Wang, C Liu, Y Xiong, J Zhang, W Zhu","doi":"10.16250/j.32.1915.2024200","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024200","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effectiveness of the integrated schistosomiasis control programme in Wuhan City from 2005 to 2023, so as to provide insights into precision control and elimination of schistosomiasis.</p><p><strong>Methods: </strong>The integrated measures for schistosomiasis control implemented by health, agriculture, water resources, and forestry departments of Wuhan City, and the epidemiological data of schistosomiasis in Wuhan City were collected from 2005 to 2023, and the prevalence of human schistosomiasis, prevalence of <i>Schistosoma japonicum</i> infections in humans and bovines, areas of <i>S. japonicum</i>-infected snail habitats, areas of snail habitats in inner embankments, and actual areas of snail habitats were retrieved. In addition, the trends in prevalence of schistosomiasis in humans and livestock and snail status were evaluated in Wuhan City from 2005 to 2023 using Mann-Kendall test and a Joinpoint regression model.</p><p><strong>Results: </strong>Mann-Kendall test revealed a tendency towards a decline in the prevalence of human schistosomiasis (<i>Z</i> = -4.41, <i>P</i> < 0.01), prevalence of <i>S. japonicum</i> infections in humans (<i>Z</i> = -4.89, <i>P</i> < 0.01) and bovines (<i>Z</i> = -4.50, <i>P</i> < 0.01), areas of <i>S. japonicum</i>-infected snail habitats (<i>Z</i> = -3.91, <i>P</i> < 0.01), areas of snail habitats in inner embankments (<i>Z</i> = -2.28, <i>P</i> = 0.02), and actual areas of snail habitats (<i>Z</i> = -5.95, <i>P</i> < 0.01) in Wuhan City from 2005 to 2023. Joinpoint regression analysis showed an average annual reduction of 8.58% in the prevalence of human schistosomiasis in Wuhan City from 2005 to 2023 [average annual percent change (AAPC) = -8.58%, 95% confidence interval (<i>CI</i>): (-10.02%, -6.65%), <i>P</i> < 0.01], with two joinpoints in 2013 and 2016, respectively, and the tendency towards a decline showed statistical significance during the period from 2013 through 2016 [annual percent change (APC) = -34.41%, 95% <i>CI</i>: (-40.36%, -20.01%), <i>P</i> < 0.01]. The prevalence of <i>S. japonicum</i> human infections appeared an average annual reduction of 51.91% in Wuhan City from 2005 to 2023 [AAPC = -51.91%, 95% <i>CI</i>: (-58.12%, -44.25%), <i>P</i> < 0.01], with two joinpoints in 2014 and 2017, respectively, and the tendency towards a decline showed statistical significance during the period from 2014 through 2017 [APC = -98.17%, 95% <i>CI</i>: (-99.17%, -90.87%), <i>P</i> < 0.01]. The prevalence of <i>S. japonicum</i> infections in bovines appeared an average annual reduction of 53.12% in Wuhan City from 2005 to 2023 [AAPC = -53.12%, 95% <i>CI</i>: (-59.65%, -42.44%), <i>P</i> < 0.01], with two joinpoints in 2011 and 2014, respectively, and the tendency towards a decline showed statistical significance during the period from 2014 through 2017 [APC = -98.63%, 95% <i>CI</i>: (-99.44%, -90.93%), <i>P</i> < 0.01]. The areas of <i>S. japonicum</i>-infected","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"176-183"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Seroprevalence of antibody against <i>Toxoplasma gondii</i> among patients with hematological malignancies].","authors":"Y Yang, Q Wang, L Xiang, Y Meng, C Zhang, J Fu","doi":"10.16250/j.32.1915.2025044","DOIUrl":"https://doi.org/10.16250/j.32.1915.2025044","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the seroprevalence of antibody against <i>Toxoplasma gondii</i> among patients with hematological malignancies, and compare it with that among health individuals, so as to provide insights into unraveling the pathogenesis of hematological malignancies.</p><p><strong>Methods: </strong>A total of 225 patients with hematological malignancies in Department of Hematology, Xuzhou Central Hospital and 300 healthy individuals in the same hospital were enrolled from 2017 to 2024. Blood samples were collected from all subjects, and the serum IgG and IgM antibodies against <i>T. gondii</i> were detected using chemiluminescent immunoassay. Demographic and clinical features were collected from patients with hematological malignancies, including gender, age, contact with cats, consumption of raw or undercooked meat, type of malignancy, clinical symptoms, blood transfusion and treatment, and the seroprevalence of anti-<i>T. gondii</i> antibody was compared among patients with different characteristics.</p><p><strong>Results: </strong>The age (<i>t</i> = 0.72, <i>P</i> > 0.05) and gender (χ² = 0.93, <i>P</i> > 0.05) were compared between patients with hematological malignancies and healthy individuals. The seroprevalence of <i>T. gondii</i> infection was 20.89% among patients with hematological malignancies and 4.33% among healthy individuals (χ² = 34.81, <i>P</i> < 0.01), and the seroprevalence of anti-<i>T. gondii</i> IgG antibody was 20.89% among patients with hematological malignancies and 4.33% among healthy individuals (χ² = 34.81, <i>P</i> < 0.01), while there was no significant difference in the seroprevalence of anti-<i>T. gondii</i> IgM antibody between patients with hematological malignancies and healthy individuals (1.33% vs. 0; corrected χ² = 2.02, <i>P</i> > 0.05). The seroprevalence of <i>T. gondii</i> infection was 23.08% among patients with leukemia, 16.67% among patients with lymphoma, 19.23% among patients with multiple myeloma, 24.00% among patients with myeloproliferative neoplasm, and 26.09% among patients with myelodysplastic syndrome (χ² = 1.44, <i>P</i> > 0.05), and was all higher than among healthy individuals (corrected χ² = 23.92, 10.74, 13.76, 12.84 and 14.54; all <i>P</i> values < 0.01). In addition, there were no significant differences in the detection of anti-<i>T. gondii</i> antibody among patients with hematological malignancies in terms of gender, age, contact with cats, consumption of raw or undercooked meat, chemotherapy or blood transfusion (χ² = 0.76, 1.97, 0, 2.81, 2.38 and 0.66; all <i>P</i> values > 0.05).</p><p><strong>Conclusions: </strong>There is a high risk of <i>T. gondii</i> infection among patients with hematological malignancies, and intensified surveillance of <i>T. gondii</i> infection is recommended among patients with hematological malignancies.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 1","pages":"93-97"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of <i>Toxoplasma gondii</i> type I/II rhoptry protein 16 on the polarization and inflammatory response of mouse alveolar macrophages].","authors":"J Li, T Dang, Z Zhao","doi":"10.16250/j.32.1915.2024199","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024199","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the effects of &lt;i&gt;Toxoplasma gondii&lt;/i&gt; type I and II rhoptry protein 16 (ROP16) on the polarization and inflammatory response of mouse alveolar macrophages, so as to provide the scientific evidence for unveiling the immunoregulatory mechanisms following &lt;i&gt;T. gondii&lt;/i&gt; infection in host cells and the clinical diagnosis and treatment of pulmonary toxoplasmosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Mouse alveolar macrophages served as blank controls, and mouse alveolar macrophages transfected with the empty lentiviral expression vector served as negative controls, and mouse alveolar macrophages transfected with lentiviral vectors overexpressing &lt;i&gt;T. gondii&lt;/i&gt; type I and II ROP16 served as the type I and II ROP16 overexpression groups. Following puromycin selection, stably transfected cells that overexpressed type I and II ROP16 were generated, observed for green fluorescence expression under a fluorescence microscope and verified using PCR, Western blotting and real-time quantitative reverse transcription PCR (RT-qPCR) assays. The expression of ROP16, inducible nitric oxide synthase (iNOS), arginase (Arg)-1, mannose receptor (CD206), cluster of differentiation 86 (CD86), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), caspase-1, apoptosis-associated speck-like protein containing a CARD (ASC), and interleukin (IL)-1β proteins was determined in mouse alveolar macrophages using Western blotting assay, and the mRNA levels of &lt;i&gt;ROP16&lt;/i&gt;, &lt;i&gt;iNOS&lt;/i&gt;, &lt;i&gt;IL-1β&lt;/i&gt;, &lt;i&gt;IL-4&lt;/i&gt;, &lt;i&gt;IL-12&lt;/i&gt;, &lt;i&gt;IL-18&lt;/i&gt;, &lt;i&gt;Arg-1&lt;/i&gt;, &lt;i&gt;IL-10&lt;/i&gt;, &lt;i&gt;IL-6&lt;/i&gt;, tumor necrosis factor (&lt;i&gt;TNF&lt;/i&gt;)-&lt;i&gt;α&lt;/i&gt; and transforming growth factor (&lt;i&gt;TGF&lt;/i&gt;)-&lt;i&gt;β&lt;/i&gt; were detected in mouse alveolar macrophages using RT-qPCR assay.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Fluorescence microscopy showed 90% of mouse alveolar macrophages producing green fluorescent signals in the type Iand II ROP16 overexpression groups and the negative control group. The relative ROP16 protein expression was 1.000 ± 0.000, 1.003 ± 0.020, 1.349 ± 0.055, and 1.376 ± 0.080 in mouse alveolar macrophages in the blank control group, negative control group, and type Iand IIROP16 overexpression groups (&lt;i&gt;F&lt;/i&gt; = 35.30, &lt;i&gt;P&lt;/i&gt; &lt; 0.01), and the relative &lt;i&gt;ROP16 mRNA&lt;/i&gt; expression was 1.007 ± 0.172, 2.030 ± 0.356, 1 409.579 ± 75.960, and 1 413.581 ± 27.712 in the blank control group, negative control group, and type Iand II ROP16 overexpression groups (&lt;i&gt;F&lt;/i&gt; = 811.00, &lt;i&gt;P&lt;/i&gt; &lt; 0.01). The ROP16 expression was significantly higher in the type Iand IIROP16 overexpression groups than in the blank control group at both protein and mRNA levels (all &lt;i&gt;P&lt;/i&gt; value &lt; 0.01). Western blotting assay detected significant differences among the four groups in terms of iNOS, Arg-1, CD86, CD206, NLRP3, caspase-1, ASC, and IL-1β protein expression (&lt;i&gt;F&lt;/i&gt; = 124.70, 82.40, 79.82, 919.40, 84.74, 39.85, 2 354.00 and 65.96, all &lt;i&gt;P&lt;/i&gt; values &lt; 0.05), and th","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"127-135"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Degradation dynamics and residue determination of pyriclobenzuron in rice and paddy environments].","authors":"H Wu, J Wang, K Yang, X Wang, N Fang, L Duan, C Zhang, X Wang","doi":"10.16250/j.32.1915.2024164","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024164","url":null,"abstract":"<p><strong>Objective: </strong>To establish a method for determination of pyriclobenzuron (PBU) residues in rice and paddy environments, and to determine the residual amounts and observe the degradation dynamics of PBU.</p><p><strong>Methods: </strong>In July 2022, the paddies of Zhejiang Academy of Agricultural Sciences were selected as experimental fields, and were divided into the blank control group (no pesticide application), the 1-fold-concentration pesticide group (1 kg/667 m²), and the 5-fold-concentration pesticide group (5 kg/667 m²), with a 100 m² area in each group. At the early tillering stage of rice, 20% suspension of PBU sulfate was sprayed once in the 1-fold-concentration and 5-fold-concentration pesticide groups, and rice plants, paddy water and soil samples were collected 2 h, and 1, 2, 3, 5, 7, 11, 14, 21, 28, 35, 49 d and 63 d following spraying PBU, while rice straw, field soil, brown rice and rice husk samples were collected 98 d following spraying. PBU was extracted and purified in samples using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) pretreatment technique, and the PBU contents were determined in samples using ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The solvent standard working solution and matrix standard working solution were prepared. A linear regression equation was fitted between PBU concentration (x-axis) and peak area (y-axis), and the ratio of the slope (<i>k</i>) of the matrix standard curve to the slope (<i>K</i>) of the solvent standard curve was calculated to evaluate the matrix effect of PBU in samples. According to the <i>Guidelines for Pesticide Residue Testing in Crops (NY/T 788</i>-<i>2018)</i>, the addition levels of PBU were set at 0.005, 0.050, 5.000, 1 000.000 mg/kg in rice plants, 0.005, 0.050, 2.000, 10.000 mg/kg in paddy water, 0.005, 0.050, 2.000 mg/kg in soil, and 0.005, 0.050, 5.000 mg/kg in brown rice and rice husks. The recovery and relative standard deviation (RSD) of PBU addition were calculated to evaluate the effectiveness of UPLC-MS/MS for determination of PBU contents. The first-order kinetic equation of PBU concentration was fitted in samples at different sampling time points to analyze the trends in PBU degradation in rice plants, paddy water, and soil, and the half-life of PBU was calculated in different samples.</p><p><strong>Results: </strong>There was a good linear relationship between the mass concentration and peak area of PBU at concentrations of 0.000 1 to 0.020 0 mg/kg under solvent and matrix conditions (<i>R</i>² = 0.985 8 to 0.999 7, <i>t</i> = -0.47 to 1.62, all <i>P</i> values < 0.01). The matrix effects of PBU were 70.26%, 65.42% and 65.12% in rice plants, brown rice and rice husks, indicating a matrix-inhibitory effect, and the matrix effect was 87.06% in soils, indicating a weak matrix effect. The recovery of PBU addition was 77.61% to 100.12% in different sample","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"201-208"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Etiological characteristics and molecular evolution of the first mpox case in Huai'an City of Jiangsu Province].","authors":"P Yang, F He, Q Yan, H Geng, T Gao, Q Gao, C Xiong, H Peng","doi":"10.16250/j.32.1915.2024260","DOIUrl":"10.16250/j.32.1915.2024260","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the virus subtypes, molecular evolutional and molecular transmission network features of the first confirmed mpox case in Huai'an City, Jiangsu Province, so as to provide insights into understanding of the transmission and evolution dynamics of mpox virus and formulation of the mpox control strategy in the city.</p><p><strong>Methods: </strong>Genomic DNA was extracted from swabs of the first confirmed mpox case's skin lesions in Huai'an City, and the amplicon sequencing library was constructed using the hypersensitive mpox virus whole-genome capture kit. High-throughput sequencing was performed using the GridION X5 nanopore sequencer on the Nanopore sequencing platform, and single nucleotide polymorphism (SNP) analysis of mpox virus genome sequences was performed following sequence assembly. In addition, phylogenetic analysis, genetic genealogy and molecular traceability analysis were performed.</p><p><strong>Results: </strong>The virus whole genome sequence of the first confirmed mpox case was successfully obtained by high-throughput sequencing, with a full length of 197 182 bp, and was named hMpxV/China/JS-HA01/2023, which belonged to the clade IIb (West African clade) lineage B.1.3. Compared with the mpox virus reference sequence MPXV-M5312_HM12_Rivers-001 (GenBank accession number: NC_063383), the genome sequence of the Huai'an virus isolate carried 86 SNPs, including 40 SNPs in the coding region as non-synonymous mutations and 73 SNPs as nucleotide mutations caused by APOBEC3 (APOBEC3). Of the 97 mpox virus gene sequences, 79 sequences were included in the molecular network (81.44%), and the threshold of the genetic distance accessed to the network was 0.35/10⁵. There were two large molecular transmission clusters and one scattered cluster in the molecular transmission network of the mpox virus, andthehMpxV/China/JS-HA01/2023 sequence was located in the large cluster. The 97 gene sequences formed 92 haplotypes, including three shared haplotypes Hap_4, Hap_6 and Hap_38, and an exclusive haplotype Hap_1 of hMpxV/China/JS-HA01/2023 generated from mutation of the exclusive haplotype Hap_43, while the exclusive haplotype Hap_43 was generated from mutation of the shared haplotype Hap_38.</p><p><strong>Conclusions: </strong>The whole genome sequence of the mpox virus isolated from the first confirmed mpox case in Huai'an City has been successfully obtained, and the molecular evolutionary and molecular transmission network characteristics of the virus have been preliminarily understood.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 1","pages":"85-92"},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on techniques for detection of tick-borne encephalitis virus infections].","authors":"Z Liu, H Xie, X Sun, T Xia, J Guo","doi":"10.16250/j.32.1915.2024191","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024191","url":null,"abstract":"<p><p>Tick-borne encephalitis is a central nervous system disease caused by infections with tick-borne pathogens, which is characterized by severe clinical symptoms, multiple sequelae, and a high fatality rate. Currently, there is no cure for tick-borne encephalitis. Tick-borne encephalitis virus (TBEV) is the most common pathogen of tick-borne encephalitis. Therefore, rapid and accurate detection of TBEV contributes to reducing the mortality of tick-borne encephalitis, improving patients' prognosis, and reducing the risk of TBEV transmission. The currently available serological tests for detection of TBEV infections mainly include neutralization test, enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay, and nucleic acid tests mainly include polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), reverse transcription polymerase spiral reaction, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas)-based assays. This review summarizes the progress of researches on serological and nucleic acid tests for detection of TBEV infections, so as to provide insights into prevention and control of tick-borne encephalitis.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"209-216"},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Current status and challenges of zoonosis prevention and control: a One Health perspective].","authors":"C Li, F Chen, S Lü","doi":"10.16250/j.32.1915.2024105","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024105","url":null,"abstract":"<p><p>Zoonosis prevention and control is a complex public health concern, which requires the collaboration of multiple regions, disciplines, and departments to enhance the effectiveness. The One Health concept aims to achieve the joint health security of humans, animals and environments through cross-disciplinary, cross-sector and cross-field collaborations. This review summarizes the development of One Health and the successful practices in the prevention and control of echinococcosis, rabies, COVID-19 and schistosomiasis, as well as explores the challenges faced in applying this concept to the prevention and control of zoonoses, so as to provide insights into formulation of the integrated zoonoses control strategy and implementation of zoonoses control interventions at the human-animal-environment interface.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 1","pages":"98-103"},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}