中国血吸虫病防治杂志最新文献

{"title":"[Dynamics of eosinophil infiltration and microglia activation in brain tissues of mice infected with <i>Angiostrongylus cantonensis</i>].","authors":"F Wei, R Zhang, Y Hu, X Qin, Y Guo, X Mo, Y Lu, J Sun, Y Zhou, J Guo, P Song, Y Chu, B Xu, T Zhang, Y Cai, M Chen","doi":"10.16250/j.32.1915.2024242","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024242","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the changes in eosinophil counts and the activation of microglial cells in the brain tissues of mice at different stages of &lt;i&gt;Angiostrongylus cantonensis&lt;/i&gt; infection, and to examine the role of microglia in regulating the progression of angiostrongyliasis and unravel the possible molecular mechanisms.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Fifty BALB/c mice were randomly divided into the control group and the 7-d, 14-d, 21-day and 25-d infection groups, of 10 mice in each group. All mice in infection groups were infected with 30 stage III &lt;i&gt;A. cantonensis&lt;/i&gt; larvae by gavage, and animals in the control group was given an equal amount of physiological saline. Five mice were collected from each of infection groups on days 7, 14, 21 d and 25 d post-infection, and 5 mice were collected from the control group on the day of oral gavage. The general and focal functional impairment was scored using the Clark scoring method to assess the degree of mouse neurological impairment. Five mice from each of infection groups were sacrificed on days 7, 14, 21 d and 25 d post-infection, and 5 mice from the control group were sacrificed on the day of oral gavage. Mouse brain tissues were sampled, and the pathological changes of brain tissues were dynamically observed using hematoxylin and eosin (HE) staining. Immunofluorescence staining with eosinophilic cationic protein (ECP) and ionized calcium binding adaptor molecule 1 (Iba1) was used to assess the degree of eosinophil infiltration and the counts of microglial cells in mouse brain tissues in each group, and the morphological parameters of microglial cells (skeleton analysis and fractal analysis) were quantified by using Image J software to determine the morphological changes of microglial cells. In addition, the expression of M1 microglia markers Fcγ receptor III (&lt;i&gt;Fcgr3&lt;/i&gt;), Fcγ receptor IIb (&lt;i&gt;Fcgr2b&lt;/i&gt;) and CD86 antigen (&lt;i&gt;Cd86&lt;/i&gt;), M2 microglia markers Arginase 1 (&lt;i&gt;Arg1&lt;/i&gt;), macrophage mannose receptor C-type 1 (&lt;i&gt;Mrc1&lt;/i&gt;), chitinase-like 3 (&lt;i&gt;Chil3&lt;/i&gt;), and phagocytosis genes myeloid cell triggering receptor expressed on myeloid cells 2 (&lt;i&gt;Trem2&lt;/i&gt;), CD68 antigen (&lt;i&gt;Cd68&lt;/i&gt;), and apolipoprotein E (&lt;i&gt;Apoe&lt;/i&gt;) was quantified using real-time quantitative reverse transcription PCR (RT-qPCR) assay in the mouse cerebral cortex of mice post-infection.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A large number of &lt;i&gt;A. cantonensis&lt;/i&gt; larvae were seen on the mouse meninges surface post-infection, and many neuronal nuclei were crumpled and deeply stained, with a large number of bleeding points in the meninges. The median Clark scores of mouse general functional impairment were 0 (interquartile range, 0), 0 (interquartile range, 0.5), 6 (interquartile range, 1.0), 14 (interquartile range, 8.5) points and 20 (interquartile range, 9.0) points in the control group and the 7-d, 14-d, 21-d and 25-d groups, respectively (&lt;i&gt;H&lt;/i&gt; = 22.45, &lt;i&gt;P&lt;/i&gt; &lt; 0.01), and the median ","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"163-175"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Non-coding RNAs expression profile of adjacent and distant liver tissues of hepatic cystic echinococcosis lesions].","authors":"I Irshat, A Aizemaiti, M Wubulikasimu, Q Xu, A Abudusikuer, Y Wu, T Kahaer","doi":"10.16250/j.32.1915.2024216","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024216","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To analyze the differential expression of non-coding RNAs (ncRNAs) from liver tissues adjacent to hepatic cystic echinococcosis (CE) lesions and distant normal liver tissues using whole transcriptome sequencing, and perform functional annotations of differentially expressed ncRNAs, so as to explore the potential role of ncRNAs in the pathogenesis of CE.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Intraoperative liver tissue specimens adjacent to hepatic CE lesions and distant normal liver tissue specimen were sampled from patients with hepatic CE, and the expression profiles of microRNAs (miRNAs), circular RNAs (circRNAs), and long non-coding RNAs (lncRNAs) were detected using whole transcriptome sequencing. Differentially expressed genes were identified, and functional annotations were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. In addition, a circRNA/lncRNA-miRNA-messenger RNA (mRNA) competing endogenous RNA (ceRNA) network was constructed using the Cytoscape software, and the expression of hub miRNAs in the network was validated using real-time quantitative reverse transcription PCR (RT-qPCR) assay.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 41 differentially expressed miRNAs were identified between the adjacent and distal tissues of hepatic CE lesions, including 8 up-regulated and 33 down-regulated miR-NAs, which were significantly enriched in biological processes of Ras signaling and neutrophil activation. Five differentially expressed circRNAs were detected, including 3 up-regulated and 2 down-regulated circRNAs, which were significantly enriched in molecular functions of hormone signaling pathways and RNA transcription regulation. A total of 447 differentially expressed lncRNAs were identified, including 200 up-regulated and 247 down-regulated lncRNAs, which were involved in cell proliferation, immune regulation, and extracellular matrix remodeling pathways. MiRNA target analysis predicted &lt;i&gt;hsa-miR-27a-5p&lt;/i&gt;, &lt;i&gt;hsa-miR-21-3p&lt;/i&gt;, and &lt;i&gt;hsa-miR-181b-2-3p&lt;/i&gt; as hub nodes in the ceRNA network. RT-qPCR assay detected that the relative expression levels of &lt;i&gt;ENSG00000253736&lt;/i&gt;, &lt;i&gt;HAS2-AS1&lt;/i&gt;, &lt;i&gt;PCSK6&lt;/i&gt;, &lt;i&gt;hsa-miR-21-3p&lt;/i&gt;, &lt;i&gt;hsa-miR-27a-5p&lt;/i&gt;, &lt;i&gt;MIR23AHG&lt;/i&gt;, &lt;i&gt;VIPR1-AS1&lt;/i&gt;, &lt;i&gt;LINC02910&lt;/i&gt;, and &lt;i&gt;hsa-miR-181b-2-3p&lt;/i&gt; were 3.00 ± 0.25, 2.75 ± 0.33, 1.01 ± 0.51, 2.65 ± 0.41, 1.01 ± 0.29, 1.10 ± 0.31, 1.05 ± 0.27, 0.25 ± 0.49, and 2.56 ± 0.35 in adjacent tissues of hepatic CE lesions, normalized to that in distant tissues from hepatic CE lesions, respectively (&lt;i&gt;t&lt;/i&gt; = 6.21, 5.83, 7.51, 7.46, 6.12, 6.65, 7.13, 1.87 and 7.81, all &lt;i&gt;P&lt;/i&gt; values &lt; 0.01), which was consistent with whole transcriptome sequencing results.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Differentially expressed ncRNAs from adjacent and distal liver tissues of hepatic CE lesions may contribute to the pathological mechanisms of CE through mediating cell proliferation, immune evasion, and","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"152-162"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Population genetic structure of sandflies in China based on mitochondrial DNA].","authors":"Z Li, Z Wei, Z Zhou, Y Zhang","doi":"10.16250/j.32.1915.2024295","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024295","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the genetic diversity of sandfly populations in endemic areas of visceral leishmaniasis in China, so as to provide references insights into management of visceral leishmaniasis and the vector sandflies.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Sixteen sampling sites were selected from main endemic foci of visceral leishmaniasis in China from June to September 2024, including Shanxi Province, Shaanxi Province, Henan Province, Gansu Province, Sichuan Province, and Xinjiang Uygur Autonomous Region. Sandflies were captured using light traps and manual aspirators from sheep pens, chicken coops, cave dwellings, bovinesheds, and pig pens at each sampling site. A single sandfly sample was washed in phosphate-buffered saline (PBS), and genomic DNA was extracted from sandfly samples. Cytochrome oxidase subunit 1 (&lt;i&gt;COI&lt;/i&gt;) gene was amplified using PCR assay with universal primers, and analyzed and retrieved with the nucleotide sequence analysis tool (BLAST) software, and the sequence of &lt;i&gt;COI&lt;/i&gt; gene was aligned with the ClustalX 1.83 and MEGA 7.0 software. The base composition and variation site of the &lt;i&gt;COI&lt;/i&gt; gene sequence were analyzed using the software MEGA 7.0, and the number of haplotypes, total number of segregating sites, haplotype diversity, nucleotide diversity, and average nucleotide differences were calculated in the &lt;i&gt;COI&lt;/i&gt; gene sequence using the software DnaSP 5.10, followed by Tajima's &lt;i&gt;D&lt;/i&gt; test for neutrality. Haplotypes were screened using the software DnaSP 5.10, and the haplotype network map of sandfly samples was plotted using the software Network 5.0. MEGA 7.0 software was employed for gene sequence editing and alignment, and calculation of genetic distances among sandfly species sampled from different regions, and a phylogenetic tree was built with a neighbor-joining method.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 466 sandflies were captured from 16 sampling sites in China from June to September 2024, and 430 gene sequences were yielded following PCR amplification and sequencing of the &lt;i&gt;COI&lt;/i&gt; gene, with 652 to 688 bp in the length of amplification fragments. The captured sandfly samples were characterized as &lt;i&gt;Phlebotomus chinensis&lt;/i&gt;, &lt;i&gt;Sergentomyia squamirostris&lt;/i&gt;, &lt;i&gt;Se. koloshanensis&lt;/i&gt;, &lt;i&gt;Ph. sichuanensis&lt;/i&gt;, and &lt;i&gt;Ph. longiductus&lt;/i&gt; following the &lt;i&gt;COI&lt;/i&gt; gene sequence alignment in BLAST. A total of 251 haplotypes were identified in the 430 gene sequences from sandfly samples (50.5%), and the average haplotype diversity, nucleotide diversity and average number of nucleotide difference were 0.885, 0.257 and 160.761, respectively. The Tajima's &lt;i&gt;D&lt;/i&gt; values were -0.92 for sandfly populations from Yangquan City, Shanxi Province and -1.73 for sandfly populations from Sanmenxia City, Henan Province, and were all more than 0 for sandfly populations from other sampling sites. Haplotype analysis identified 50 haplotypes, which were classified into two haplogroups.","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"144-151"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Proportions of memory T cells and expression of their associated cytokines in lymph nodes of mice infected with <i>Echinococcus multilocularis</i>].","authors":"Y Li, D Adilai, B Deng, A Abidan, S Sun, W Xiao, C Ge, N Tang, J Li, H Wang, T Jiang, C Zhang","doi":"10.16250/j.32.1915.2024224","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024224","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the effects of &lt;i&gt;Echinococcus multilocularis&lt;/i&gt; infection on levels of memory T (Tm) cells and their subsets in lymph nodes of mice at different stages of infection, so as to provide new insights into immunotherapy for alveolarechinococcosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Twenty-four C57BL/6J mice aged 6 to 9 weeks were randomly divided into the infection group and the control group, of 12 mice in each group. Mice in the infection group were administered with 3 000 &lt;i&gt;E. multilocularis&lt;/i&gt; protoscoleces via portal venous injection, while animals in the control group were administered with an equal volume of physiological saline. Three mice from each group were sacrificed 4, 12 weeks and 24 weeks post-infection, and lymph nodes were sampled and stained with hematoxylin and eosin (HE) to investigate the histopathological changes of mouse lymph nodes in the infection group. The expression and localization of T lymphocyte surface markers CD3, CD4, and CD8 were observed in mouse lymph nodes using immunohistochemical staining. In addition, lymphocyte suspensions were prepared from mouse lymph nodes in both groups at different time points post-infection, and the levels of Tm cell subsets and their secreted cytokines were detected using flow cytometry.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;HE staining showed diffuse structural alterations in the subcapsular cortical and paracortical regions of mouse lymph nodes in the infection group 4 weeks post-infection with &lt;i&gt;E. multilocularis&lt;/i&gt;. Immunohistochemical staining detected CD3, CD4 and CD8 expression in mouse lymph nodes in both groups. Flow cytometry revealed higher proportions of CD4&lt;sup&gt;+&lt;/sup&gt; Tm cells [(55.3 ± 4.8)% vs. (38.8 ± 6.1)%; &lt;i&gt;t&lt;/i&gt; = -4.259, &lt;i&gt;P&lt;/i&gt; &lt; 0.05] and CD4&lt;sup&gt;+&lt;/sup&gt; tissue-resident Tm (Trm) cells [(57.7 ± 3.7)% vs. (34.1 ± 11.2)%; &lt;i&gt;t&lt;/i&gt; = -3.990, &lt;i&gt;P&lt;/i&gt; &lt; 0.05] in mouse lymph nodes in the infection group than in the control group 4 weeks post-infection, and higher proportions of CD4&lt;sup&gt;+&lt;/sup&gt; Tm cells [(34.6 ± 3.2)% vs. (23.3 ± 7.5)%; &lt;i&gt;t&lt;/i&gt; = -2.764, &lt;i&gt;P&lt;/i&gt; &lt; 0.05] and CD4&lt;sup&gt;+&lt;/sup&gt; Trm cells [(44.0 ± 1.9)% vs. (31.2 ± 1.5)%; &lt;i&gt;t&lt;/i&gt; = -4.039, &lt;i&gt;P&lt;/i&gt; &lt; 0.05] in mouse lymph nodes in the infection group than in the control group 24 weeks post-infection. The proportions of CD8&lt;sup&gt;+&lt;/sup&gt; Tm cells were higher in the infection group than in the control group 4 weeks [(56.8 ± 2.7)% vs. (43.9 ± 5.2)%; &lt;i&gt;t&lt;/i&gt; = -4.416, &lt;i&gt;P&lt;/i&gt; &lt; 0.01] and 12 weeks post-infection [(25.4 ± 2.7)% vs. (12.0 ± 2.6)%; &lt;i&gt;t&lt;/i&gt; = -2.552, &lt;i&gt;P&lt;/i&gt; &lt; 0.05], while the proportions of tumor necrosis factor (TNF)-α&lt;sup&gt;+&lt;/sup&gt; CD4&lt;sup&gt;+&lt;/sup&gt; T cells [(15.7 ± 5.0)% vs. (49.4 ± 6.4)%; &lt;i&gt;t&lt;/i&gt; = 7.150, &lt;i&gt;P&lt;/i&gt; &lt; 0.01], TNF-α&lt;sup&gt;+&lt;/sup&gt;CD8&lt;sup&gt;+&lt;/sup&gt; T cells [(20.7 ± 5.5)% vs. (57.5 ± 8.4)%; &lt;i&gt;t&lt;/i&gt; = -6.694, &lt;i&gt;P&lt;/i&gt; &lt; 0.01], and TNF-α&lt;sup&gt;+&lt;/sup&gt; CD8&lt;sup&gt;+&lt;/sup&gt; Tm cells [7.0% (1.0%) vs. 31.0% (11.0%); &lt;i&gt;Z&lt;/i&gt; = -2.236, &lt;i&gt;P&lt;/i&gt; &lt; 0.05] were l","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"136-143"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Sequencing and analysis of the complete mitochondrial genome of <i>Bulinus globosus</i>].","authors":"P Qian, M-M Masceline Jenipher, C Lü, Y Qian, W Wang, S Chen, A Xu, J Xue, J Xu, X Zhou, M Nicholas, S Li","doi":"10.16250/j.32.1915.2024262","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024262","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the structural and phylogenetic characteristics of the mitochondrial genome from <i>Bulinus globosus</i>, so as to provide a theoretical basis for classification and identification of species within the <i>Bulinus</i> genus, and to provide insights into understanding of <i>Bulinus</i>-schistosomes interactions and the mechanisms of parasite transmission.</p><p><strong>Methods: </strong><i>B. globosus</i> samples were collected from the Ruya River basin in Zimbabwe. Mitochondrial DNA was extracted from <i>B. globosus</i> samples and the corresponding libraries were constructed for high-throughput sequencing on the Illumina NovaSeq 6000 platform. After raw sequencing data were subjected to quality control using the fastp software, genome assembly was performed using the A5-miseq and SPAdes tools, and genome annotation was conducted using the MITOS online server. Circular maps and sequence plots of the mitochondrial genome were generated using the CGView and OGDRAW software, and the protein conservation motifs and structures were analyzed using the TBtools software. Base composition and codon usage bias were analyzed and visualized using the software MEGA X and the ggplot2 package in the R software. In addition, a phylogenetic tree was created in the software MEGA X after sequence alignment with the software MAFFT 7, and visualized using the software iTOL.</p><p><strong>Results: </strong>The mitochondrial genome of <i>B. globosus</i> was a 13 730 bp double-stranded circular molecule, containing 2 ribosomal RNA (<i>rRNA</i>) genes, 22 transfer RNA (<i>tRNA</i>) genes, and 13 protein-coding genes, with a marked AT preference. The mitochondrial genome composition of <i>B. globosus</i> was similar to that of other species within the <i>Bulinus</i> genus. Phylogenetic analysis revealed that the complete mitochondrial genome sequence of <i>B. globosus</i> was clustered with <i>B. truncatus</i>, <i>B. nasutus</i>, and <i>B. ugandae</i> into the same evolutionary clade, and gene superfamily analysis showed that the metabolism-related proteins of <i>B. globosus</i> were highly conserved, notably the cytochrome c oxidase family, which showed a significant consistency.</p><p><strong>Conclusions: </strong>This is the first whole mitochondrial genome sequencing to decode the compositional features of the mitochondrial genome of <i>B. globosus</i> from Zimbabwe and its evolutionary relationship within the <i>Bulinus</i> genus, which provides important insights for further understanding of the phylogeny and mitochondrial genome characteristics of the <i>Bulinus</i> genus.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"116-126"},"PeriodicalIF":0.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The future is already here and the wave is coming: progress and challenges in the research and application of artificial intelligence-empowered malaria control programmes].","authors":"G Zhu, J Cao","doi":"10.16250/j.32.1915.2025062","DOIUrl":"https://doi.org/10.16250/j.32.1915.2025062","url":null,"abstract":"<p><p>The rapid rise and fast development of artificial intelligence (AI) has brought unprecedented opportunities and challenges to all sectors, including disease prevention control. Malaria is one of the world's most devastating infectious diseases. This article summarizes the advances in the research and application of AI-empowered malaria control programmes, analyzes key challenges during the implementation of malaria control programmes, and proposes future development directions and research proprieties, so as to provide insights into facilitating the translation of AI-driven strategies in global infectious disease control efforts.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"112-115"},"PeriodicalIF":0.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Investigation of tick species in Suizhou City, Hubei Province from 2023 to 2024].","authors":"H Lu, F Guo, Y Pan, M Peng, L Wu, Y Lin, X Liu, X Yu","doi":"10.16250/j.32.1915.2024185","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024185","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the species of ticks in Suizhou City, Hubei Province, so as to provide insights into management of ticks and tick-borne diseases.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;During the period between May 2023 and June 2024, livestock breeding farms and vegetation neighboring the place of residence of confirmed and suspected patients with tick-borne disease were selected as sampling points in rural areas from Yindian Township, Gaocheng Township, Wanhe Township, Wushan Township, Xiaolin Township, Xihe Township, Hedian Township and Beijiao Street in Suizhou City, Hubei Province, where confirmed and suspected cases with tick-borne diseases had been reported. The parasitic ticks on the body surface of free-range livestock were captured with tweezers in livestock breeding farms, and free ticks on the vegetation surface were captured with the flagging method. Morphological identification of tick samples was performed under a microscope, and the gender and developmental stage of ticks were determined. One engorged adult tick, 2 to 3 blood-feeding but non-engorged adult ticks, 10 to 15 unfed female ticks, 15 to 20 unfed male ticks, and 30 to 40 tick nymphs or larvae were assigned into a group, respectively. Genomic DNA was extracted from tick samples in each group, and mitochondrial &lt;i&gt;16S rRNA&lt;/i&gt; gene was amplified. Sequence analysis was performed with the DNASTAR software, and phylogenetic analysis was performed using the software MEGA 7.0. In addition, the phylogenetic tree was generated using the maximum likelihood method based on the Kimura 2 parameter model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 2 438 ticks were captured from Suizhou City, Hubei Province during the period between May 2023 and June 2024, including 595 free ticks and 1 483 parasitic ticks. Three developmental stages of ticks were captured, including larvae, nymphs, and adults, and 75.18% (1 899/2 438) of captured ticks were adult, in which 79.04% (1 501/1 899) were female. Morphological and molecular biological assays identified one family, three genera and four species of captured ticks, including 2 425 &lt;i&gt;Haemaphysalis longicornis&lt;/i&gt; ticks (99.47%) and one &lt;i&gt;H. flava&lt;/i&gt; tick (0.04%) of the genus &lt;i&gt;Haemaphysalis&lt;/i&gt;, 11 &lt;i&gt;Rhipicephalus microplus&lt;/i&gt; ticks (0.45%) of the genus &lt;i&gt;Rhipicephalus&lt;/i&gt;, and one &lt;i&gt;Ixodes sinensis&lt;/i&gt; tick (0.04%) of the genus &lt;i&gt;Ixodes&lt;/i&gt; in the family Ixodidae. Phylogenetic analysis revealed that the &lt;i&gt;H. longicornis&lt;/i&gt; sequence (SZ49) in this study was clustered with sequences from Yunnan Province (GenBank accession number: MH024510.1), Hebei Province (GenBank accession number: MK450606.1) and Henan Province (GenBank accession number: MZ230645.1) into a clade, and the &lt;i&gt;H. flava&lt;/i&gt; sequence (SZ19) in this study was clustered with sequences from Japan (GenBank accession number: MW064044.1), South Korea (GenBank accession number: ON629585.1), and Jiangsu Province (GenBank accession number: PP494741.1) and H","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"184-189"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Strategic thinking on management of cross-boundary imported schistosomiasis].","authors":"J Xu, S Li, Q Li, S Guo, S Li, X Zhou","doi":"10.16250/j.32.1915.2025018","DOIUrl":"https://doi.org/10.16250/j.32.1915.2025018","url":null,"abstract":"<p><p>Schistosomiasis is prevalent in 78 countries and territories worldwide, while the eastern and western parts of sub-Sahara Africa bear the highest disease burden due to schistosomiasis. Recently, climate change, international trade and travel, urbanization and war have increased the risk of cross-boundary importation and transmission of schistosomiasis, and schistosomiasis has increasingly become a public health concern in non-endemic countries and territories. Biomphalaria straminea, the intermediate host of Schistosoma mansoni, has colonized in southern China and its habitats continue to move northward. In addition, cross-boundary imported cases of schistosomiasis have been reported occasionally in China. However, the real number of cases may be underestimated greatly due to insufficient diagnostic capacity and weak awareness of case reporting for overseas imported schistosomiasis in healthcare facilities. It is necessary to establish a multi-party collaborative mechanism, improve corresponding systems and technical specifications, reinforce surveillance and early warning, and border management, enhance technical reserves and capability building, and improve the awareness of schistosomiasis prevention and healthcare-seeking among entry-exit personnel, in order to effectively address the threat of cross-boundary imported schistosomiasis.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"107-111"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Current status of anticoccidial drug resistance in China].","authors":"Y Bai, W Li, W Luo, Y Yu, D Li, J Zhao, L He","doi":"10.16250/j.32.1915.2024147","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024147","url":null,"abstract":"<p><p>Avian coccidiosis, an acute parasitic disease that mainly harms chicks, is widely prevalent across the world, which poses a serious threat to poultry industry. Because of the single prophylactic formulations, veterinary clinical treatment of coccidiosis mainly relies on chemically synthesized agents, polyether ionophores and Chinese herbal medicines. The introduction of novel anticoccidial drugs is slow for a long period of time, and there is an increasing problem of anticoccidial drug resistance following long-term use, which has become an urgent problem to be solved in poultry industry. This review summarizes the levels of anticoccidial drug resistance across China from 2018 to 2023, and analyzes the resistance to various anticoccidial agents in coccidia. It is indicated that the overall prevalence of anticoccidial drug resistance is high in coccidia, and development of novel anticoccidial agents and products with reduced antibiotics use and alternatives of antibiotics is of an urgent need.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"217-222"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effectiveness of the integrated schistosomiasis control programme in Wuhan City from 2005 to 2023].","authors":"S Wang, H Luo, Y Li, H Wang, C Liu, Y Xiong, J Zhang, W Zhu","doi":"10.16250/j.32.1915.2024200","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024200","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To evaluate the effectiveness of the integrated schistosomiasis control programme in Wuhan City from 2005 to 2023, so as to provide insights into precision control and elimination of schistosomiasis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The integrated measures for schistosomiasis control implemented by health, agriculture, water resources, and forestry departments of Wuhan City, and the epidemiological data of schistosomiasis in Wuhan City were collected from 2005 to 2023, and the prevalence of human schistosomiasis, prevalence of &lt;i&gt;Schistosoma japonicum&lt;/i&gt; infections in humans and bovines, areas of &lt;i&gt;S. japonicum&lt;/i&gt;-infected snail habitats, areas of snail habitats in inner embankments, and actual areas of snail habitats were retrieved. In addition, the trends in prevalence of schistosomiasis in humans and livestock and snail status were evaluated in Wuhan City from 2005 to 2023 using Mann-Kendall test and a Joinpoint regression model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Mann-Kendall test revealed a tendency towards a decline in the prevalence of human schistosomiasis (&lt;i&gt;Z&lt;/i&gt; = -4.41, &lt;i&gt;P&lt;/i&gt; &lt; 0.01), prevalence of &lt;i&gt;S. japonicum&lt;/i&gt; infections in humans (&lt;i&gt;Z&lt;/i&gt; = -4.89, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) and bovines (&lt;i&gt;Z&lt;/i&gt; = -4.50, &lt;i&gt;P&lt;/i&gt; &lt; 0.01), areas of &lt;i&gt;S. japonicum&lt;/i&gt;-infected snail habitats (&lt;i&gt;Z&lt;/i&gt; = -3.91, &lt;i&gt;P&lt;/i&gt; &lt; 0.01), areas of snail habitats in inner embankments (&lt;i&gt;Z&lt;/i&gt; = -2.28, &lt;i&gt;P&lt;/i&gt; = 0.02), and actual areas of snail habitats (&lt;i&gt;Z&lt;/i&gt; = -5.95, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) in Wuhan City from 2005 to 2023. Joinpoint regression analysis showed an average annual reduction of 8.58% in the prevalence of human schistosomiasis in Wuhan City from 2005 to 2023 [average annual percent change (AAPC) = -8.58%, 95% confidence interval (&lt;i&gt;CI&lt;/i&gt;): (-10.02%, -6.65%), &lt;i&gt;P&lt;/i&gt; &lt; 0.01], with two joinpoints in 2013 and 2016, respectively, and the tendency towards a decline showed statistical significance during the period from 2013 through 2016 [annual percent change (APC) = -34.41%, 95% &lt;i&gt;CI&lt;/i&gt;: (-40.36%, -20.01%), &lt;i&gt;P&lt;/i&gt; &lt; 0.01]. The prevalence of &lt;i&gt;S. japonicum&lt;/i&gt; human infections appeared an average annual reduction of 51.91% in Wuhan City from 2005 to 2023 [AAPC = -51.91%, 95% &lt;i&gt;CI&lt;/i&gt;: (-58.12%, -44.25%), &lt;i&gt;P&lt;/i&gt; &lt; 0.01], with two joinpoints in 2014 and 2017, respectively, and the tendency towards a decline showed statistical significance during the period from 2014 through 2017 [APC = -98.17%, 95% &lt;i&gt;CI&lt;/i&gt;: (-99.17%, -90.87%), &lt;i&gt;P&lt;/i&gt; &lt; 0.01]. The prevalence of &lt;i&gt;S. japonicum&lt;/i&gt; infections in bovines appeared an average annual reduction of 53.12% in Wuhan City from 2005 to 2023 [AAPC = -53.12%, 95% &lt;i&gt;CI&lt;/i&gt;: (-59.65%, -42.44%), &lt;i&gt;P&lt;/i&gt; &lt; 0.01], with two joinpoints in 2011 and 2014, respectively, and the tendency towards a decline showed statistical significance during the period from 2014 through 2017 [APC = -98.63%, 95% &lt;i&gt;CI&lt;/i&gt;: (-99.44%, -90.93%), &lt;i&gt;P&lt;/i&gt; &lt; 0.01]. The areas of &lt;i&gt;S. japonicum&lt;/i&gt;-infected","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 2","pages":"176-183"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}