中国血吸虫病防治杂志最新文献

{"title":"[Prevalence and genetic characteristics of <i>Cryptosporidium</i> infections among HIV-positive individuals in Jiangxi Province].","authors":"Z Hu, L Lu, Y Yu, L Li, W Wang, G Fan, C Feng, Y Zheng, G Peng","doi":"10.16250/j.32.1374.2024071","DOIUrl":"10.16250/j.32.1374.2024071","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prevalence of <i>Cryptosporidium</i> infection and the distribution of parasite species and genotypes among HIV-positive individuals in Jiangxi Province.</p><p><strong>Methods: </strong>HIV-positive individuals' sociodemographic and clinical data were collected from three AIDS designated hospitals in Jiangxi Province from January 2022 to March 2023. Subjects' stool samples were collected, and genomic DNA was extracted from stool samples. Nested PCR assay was performed based on the small subunit ribosomal RNA (<i>SSU rRNA</i>) gene of <i>Cryptosporidium</i>, and <i>Cryptosporidium gp60</i> gene was amplified in stool samples positive for the <i>SSU rRNA</i> gene. The second-round PCR amplification product was checked with 1.5% agarose gel electrophoresis, and the products of suspected positive amplifications were sequenced, followed by sequence alignment. The phylogenetic tree was created using the Neighbor-Joining method with the software MEGA 11.0, to characterize the species, genotypes and sub-genotypes of <i>Cryptosporidium</i>.</p><p><strong>Results: </strong>A total of 382 HIV-positive individuals were enrolled, with two cases identified with <i>Cryptosporidium</i> infection (0.52% prevalence), and both cases had no abdominal pain or diarrhea. Following sequencing and sequence alignment, the gene sequences of these two <i>Cryptosporidium</i> isolates shared 99.76% and 99.88% similarity with the gene sequence of <i>C. meleagridis</i> isolates. Phylogenetic analysis based on the <i>Cryptosporidium SSU rRNA</i> gene sequence identified the species of these two <i>Cryptosporidium</i>-positive stool samples as <i>C. meleagridis</i>. Following nested PCR amplification of the <i>Cryptosporidium gp60</i> gene, sequencing and sequence alignment, the two <i>C. meleagridis</i> isolates were characterized as III eA17G2R1 and III bA25G1R1a sub-genotypes, and the sub-genotype III bA25G1R1a was firstly described in humans.</p><p><strong>Conclusions: </strong>The prevalence of <i>Cryptosporidium</i> is low among HIV-positive individuals in Jiangxi Province. The likelihood of <i>Cryptosporidium</i> infection cannot be neglected among HIV-positive individuals without diarrhea.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"637-642"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and preliminary application of a multiplex PCR assay for simultaneous detection of four intestinal parasites in goats].","authors":"Y Li, X Mu, H Xu, X Luo, R Yu, X Xu, L Yang, X Yu, Y Hong","doi":"10.16250/j.32.1374.2024046","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024046","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To develop a multiplex PCR assay for simultaneous detection of four intestinal parasites, including &lt;i&gt;Giardia duodenalis&lt;/i&gt;, &lt;i&gt;Cryptosporidium parvum&lt;/i&gt;, &lt;i&gt;Enterocytozoon bieneusi&lt;/i&gt; and &lt;i&gt;Moniezia&lt;/i&gt;, and to preliminarily evaluate its detection efficiency.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Four pairs of specific primers were designed based on the conserved sequences of the corresponding genes of &lt;i&gt;G. duodenalis&lt;/i&gt; (GenBank accession number: XM_001710026.2), &lt;i&gt;C. parvum&lt;/i&gt; (GenBank accession number: XM_626998.1), &lt;i&gt;E. bieneusi&lt;/i&gt; (GenBank accession number: KJ719492.1) and &lt;i&gt;Moniezia&lt;/i&gt; (GenBank accession number: OM296991.1) retrieved from the GenBank database, and a multiplex PCR assay for simultaneous detection of &lt;i&gt;G. duodenalis&lt;/i&gt;, &lt;i&gt;C. parvum&lt;/i&gt;, &lt;i&gt;E. bieneusi&lt;/i&gt; and &lt;i&gt;Moniezia&lt;/i&gt; was developed and optimized. A total of 116 fresh goat stool samples were collected from four goat farms in Zhanjiang City, Guangdong Province during the period from October to December 2022, including 96 samples used for evaluating the detection efficacy of the multiplex PCR assay, and 20 samples as baseline controls for sample testing. Genomic DNA extracted from 96 goat stool samples was tested using the single-target PCR assay and the developed multiplex PCR assay, and the sensitivity, specificity, positive predictive value, and negative predictive value of the multiplex PCR assay were evaluated for detection of &lt;i&gt;G. duodenalis&lt;/i&gt;, &lt;i&gt;C. parvum&lt;/i&gt;, &lt;i&gt;E. bieneusi&lt;/i&gt; and &lt;i&gt;Moniezia&lt;/i&gt; DNA in goat stool samples with the single-target PCR assay as the gold standard.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The multiplex PCR assay developed in this study allowed simultaneous amplification of specific gene fragments of &lt;i&gt;G. duodenalis&lt;/i&gt;, &lt;i&gt;C. parvum&lt;/i&gt;, &lt;i&gt;E. bieneusi&lt;/i&gt; and &lt;i&gt;Moniezia&lt;/i&gt;, with 1 400, 755, 314 bp and 585 bp in sizes, respectively, and the detection limit was 10&lt;sup&gt;2&lt;/sup&gt; and higher copies of parasite DNA clones, while the multiplex PCR assay was negative for gene amplification of &lt;i&gt;Schistosoma japonicum&lt;/i&gt;, &lt;i&gt;Fasciola hepatica&lt;/i&gt;, &lt;i&gt;Echinococcus granulosus&lt;/i&gt;, &lt;i&gt;Blastocystis hominis&lt;/i&gt; and &lt;i&gt;Homalogaster paloniae&lt;/i&gt;. Single-target PCR assay and the developed multiplex PCR assay were employed to test DNA samples extracted from 96 goat stool samples, and single-target PCR assay tested positive in 40 goat stool samples (41.67%), including 39 positive samples tested with the multiplex PCR assay, with a mean coincidence rate of 97.50% (39/40). The multiplex PCR assay tested positive for &lt;i&gt;G. duodenalis&lt;/i&gt; DNA in 26 goat stool samples (27.10%), &lt;i&gt;C. parvum&lt;/i&gt; DNA in 22 samples (22.90%), &lt;i&gt;E. bieneusi&lt;/i&gt; DNA in 24 samples (25.00%), and &lt;i&gt;Moniezia&lt;/i&gt; in 9 samples (9.40%), which was consistent with the detection using the single-target PCR assay. The sensitivity, negative predictive value, and positive predictive value of the multiplex PCR assay were 96.15%, 95.83%, 100.00% and 100.0","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 4","pages":"376-383"},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Auxiliary diagnostic value of T cells spot test of <i>Mycobacterium tuberculosis</i> infection for pulmonary and extra-pulmonary tuberculosis among the elderly].","authors":"R Huang, S Li, C Wang","doi":"10.16250/j.32.1374.2024121","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024121","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the auxiliary diagnostic value of T cells spot test of <i>Mycobacterium tuberculosis</i> infection (T-SPOT.TB) for pulmonary and extra-pulmonary tuberculosis among the elderly.</p><p><strong>Methods: </strong>A total of 173 elderly patients at ages of 60 years and older and with suspected tuberculosis that were admitted to People's Hospital of Xinjiang Uygur Autonomous Region during the period from October 2022 through February 2024 were enrolled, and all patients underwent T-SPOT.TB, acid fast staining and GeneXpert MTB/RIF tests. The etiological tests of MTB served as a gold standard, and the diagnostic values of T-SPOT.TB, acid fast staining and GeneXpert MTB/RIF tests for pulmonary and extra-pulmonary tuberculosis were compared among the elderly patients.</p><p><strong>Results: </strong>Of the 173 elderly patients suspected of tuberculosis, there were 44 patients definitely diagnosed with pulmonary tuberculosis, 30 cases with extra-pulmonary tuberculosis, and 99 cases without tuberculosis. The sensitivities of T-SPOT.TB, acid fast staining and GeneXpert MTB/RIF tests were 86.5%, 27.0% and 54.1% for diagnosis of tuberculosis. The sensitivities of T-SPOT.TB were 86.4% and 86.7% for diagnosis of pulmonary tuberculosis and extra-pulmonary tuberculosis, with an 80.8% specificity for diagnosis of tuberculosis. The sensitivities of GeneXpert MTB/RIF were 56.8% and 50.0% for diagnosis of pulmonary tuberculosis and extra-pulmonary tuberculosis, with a 100.0% specificity each, and the sensitivities of acid fast staining were 31.8% and 20.0% for diagnosis of pulmonary tuberculosis and extra-pulmonary tuberculosis, with a 100.0% specificity each. In addition, the areas under the receiver operating characteristic curve were 0.836, 0.635 and 0.770 for diagnosis of tuberculosis with T-SPOT.TB, acid fast staining and GeneXpert MTB/RIF tests among the elderly patients, respectively.</p><p><strong>Conclusions: </strong>T-SPOT.TB has a high auxiliary diagnostic value for both pulmonary and extra-pulmonary tuberculosis among elderly patients.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"310-313"},"PeriodicalIF":0.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular tracing of <i>Biomphalaria straminea</i> in China].","authors":"L Duan, L Qu, Y Guo, W Gu, S Lü, Y Zhang, X Zhou","doi":"10.16250/j.32.1374.2024069","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024069","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the origin of <i>Biomphalaria straminea</i> in China, so as to provide insights into assessment of schistosomiasis mansoni transmission risk and <i>B. straminea</i> control.</p><p><strong>Methods: </strong>Guanlan River, Dasha River, Shenzhen Reservoir, upper and lower reaches of Kuiyong River, and Xinzhen River in Shenzhen, China, were selected as sampling sites. Ten <i>Biomphalaria</i> samples were collected from each site, and genomic DNA was extracted from <i>Biomphalaria</i> samples. DNA samples were obtained from 15 <i>B. straminea</i> sampled from 5 sampling sites in Minas Gerais State, Pará State, Federal District, Pernambuco State, and Sao Paulo State in Brazil, South America. Cytochrome c oxidase I (<i>COI</i>) and mitochondrial 16S ribosomal RNA (<i>16S rRNA</i>) genes were sampled using the above DNA templates, and the amplified products were sequenced. The <i>COI</i> and <i>16S rRNA</i> gene sequences were downloaded from GenBank, and the sampling sites were acquired. All <i>COI</i> and 1<i>6S rRNA</i> gene sequences were aligned and evolutionary trees of <i>B. straminea</i> were created based on <i>COI</i> and <i>16S rRNA</i> gene sequences to identify the genetic similarity and evolutionary relationship between <i>B. straminea</i> samples from China and South America.</p><p><strong>Results: </strong>A total of 60 <i>COI</i> gene sequences with a length of 529 bp and 3 haplotypes were obtained from <i>B. straminea</i> sampled from China. There were 165 <i>COI</i> gene sequences of <i>B. straminea</i> retrieved from GenBank, and following alignment with the above 60 gene sequences, a total of 33 haplotypes were obtained. Phylogenetic analysis showed that the three haplotypes of <i>B. straminea</i> from China were clustered into one clade, among which the haplotype China11 and three <i>B. straminea</i> samples from Brazil retrieved from GenBank belonged to the same haplotype. Geographical evolution analysis showed that the <i>B. straminea</i> samples from three sampling sites along eastern coasts of Brazil had the same haplotype with China11, and <i>B. straminea</i> samples from other two sampling sites were closely, genetically related to China11. A total of 60 <i>16S rDNA</i> gene sequences with approximately 322 bp in length were amplified from <i>B. straminea</i> in China, with 2 haplotypes identified. A total of 70 <i>16S rDNA</i> gene sequences of <i>B. straminea</i> were captured from GenBank. Phylogenetic analysis showed that <i>Biomphalaria</i> snails collected from China were clustered into a clade, and the haplotype China64 and the haplotype 229BS from Brazil shared the same haplotype. The 49 <i>16S rDNA</i> gene sequences of <i>B. straminea</i> from 25 sampling sites in southern Brazil, which were captured from GenBank, were included in the present analysis, and the <i>B. straminea</i> from 3 sampling sites shared the same haplotype with China64 in China. Geographical evolution","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"272-278"},"PeriodicalIF":0.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of the CRISPR/Cas system in gene editing and nucleic acid detection of parasitic diseases: a review].","authors":"S Yan, S Yang, H Yang, Y Xin, B Xu, W Hu, Y Lu, B Zheng","doi":"10.16250/j.32.1374.2024057","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024057","url":null,"abstract":"<p><p>CRISPR/Cas system, an adaptive immune system with clustered regularly interspaced short palindromic repeats, may interfere with exogenous nucleic acids and protect prokaryotes from external damages, is an effective gene editing and nucleic acid detection tools. The CRISPR/Cas system has been widely applied in virology and bacteriology; however, there is relatively less knowledge about the application of the CRISPR/Cas system in parasitic diseases. The review summarizes the mechanisms of action of the CRISPR/Cas system and provides a comprehensive overview of their application in gene editing and nucleic acid detection of parasitic diseases, so as to provide insights into future studies on parasitic diseases.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"314-320"},"PeriodicalIF":0.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of oxymatrine on <i>Cryptosporidium parvum</i> infection in mice based on the HMGB1-TLR2/TLR4-NF-κB pathway].","authors":"J Shi, R Ji, Z Guan, X Zhang, Y Lu","doi":"10.16250/j.32.1374.2024019","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024019","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the involvement of the high mobility group box protein B1 (HMGB1)-Toll-like receptor 2 (TLR2)/TLR4-nuclear factor κB (NF-κB) pathway in the intestinal mucosal injury induced by <i>Cryptosporidium parvum</i> infection, and to examine the effect of oxymatrine (OMT) on <i>C. parvum infection</i> in mice.</p><p><strong>Methods: </strong>Forty SPF 4-week-old BALB/c mice were randomly divided into four groups, including the control group, infection group, glycyrrhizin (GA) group and OMT group. Each mouse was orally administered with 1 × 10⁵ <i>C. parvum</i> oocysts one week in the infection, GA and OMT groups following dexamethasone-induced immunosuppression to model <i>C. parvum</i> intestinal infections in mice. Upon successful modeling, mice in the GA group were intraperitoneally injected with GA at a daily dose of 25.9 mL/kg for successive two weeks, and animals in the OMT group were orally administered OMT at a daily dose of 50 mg/kg for successive two weeks, while mice in the control group were given normal food and water. All mice were sacrificed two weeks post-treatment, and proximal jejunal tissues were sampled. The pathological changes of mouse intestinal mucosal specimens were observed using hematoxylin-eosin (HE) staining, and the mouse intestinal villous height, intestinal crypt depth and the ratio of intestinal villous height to intestinal crypt depth were measured. The occludin and zonula occludens protein 1 (ZO1) expression was determined in mouse intestinal epithelial cells using immunohistochemistry, and the relative expression of <i>HMGB1</i>, <i>TLR2</i>, <i>TLR4</i>, myeloid differentiation primary response gene 88 (<i>MyD88</i>) and <i>NF-κB p65 mRNA</i> was quantified in mouse jejunal tissues using quantitative real-time PCR (qPCR) assay.</p><p><strong>Results: </strong>HE staining showed that the mouse intestinal villi were obviously atrophic, shortened, and detached, and the submucosal layer of the mouse intestine was edematous in the infection group as compared with the control group, while the mouse intestinal villi tended to be structurally intact and neatly arranged in the GA and OMT groups. There were significant differences among the four groups in terms of the mouse intestinal villous height (<i>F</i> = 6.207, <i>P</i> = 0.000 5), intestinal crypt depth (<i>F</i> = 6.903, <i>P</i> = 0.000 3) and the ratio of intestinal villous height to intestinal crypt depth (<i>F</i> = 37.190, <i>P</i> < 0.000 1). The mouse intestinal villous height was lower in the infection group than in the control group [(321.9 ± 41.1) μm vs. (399.5 ± 30.9) μm; <i>t</i> = 4.178, <i>P</i> < 0.01] and the GA group [(321.9 ± 41.1) μm vs. (383.7 ± 42.7) μm; <i>t</i> = 3.130, <i>P</i> < 0.01], and the mouse intestinal crypt depth was greater in the infection group [(185.0 ± 35.9) μm] than in the control group [(128.4 ± 23.6) μm] (<i>t</i> = 3.877, <i>P</i> < 0.01) and GA group [(143.3 ± 24.7) μm] ","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"286-293"},"PeriodicalIF":0.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[How do female mosquitoes determine the most suitable males for mating?]","authors":"Y Li, D Li, X Liu, Y Wang, T Liu, Y Xu, S Deng","doi":"10.16250/j.32.1374.2023220","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023220","url":null,"abstract":"<p><p>More than 80% of the world's populations are at risk of vector-borne diseases, with mosquito-borne diseases as a significant global public health problem. Mosquito populations control is critical to interrupting the transmission of mosquito-borne diseases. This review summarizes the physical attributes, smell, vision, touch, and hearing of mosquitoes to unravel the preferences of female mosquitoes, and describes the mechanisms underlying the best male mating by female mosquitoes, so as to provide new insights into management of mosquito-borne diseases.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 3","pages":"321-328"},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preparation and preliminary application of the polyclonal antibody against <i>Toxoplasma gondii</i> dense granule protein 24].","authors":"S Fu, Y Yang, C Wang, Q Luo, L Yu","doi":"10.16250/j.32.1374.2024083","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024083","url":null,"abstract":"<p><strong>Objective: </strong>To prepare and characterize the mouse polyclonal antibody against the dense granule protein 24 (GRA24) of <i>Toxoplasma gondii</i>, and explore its preliminary applications.</p><p><strong>Methods: </strong>The GRA24 coding sequences of different <i>T. gondii</i> strains were aligned using the MEGA-X software, and the dominant peptide of the GRA24 protein was analyzed with the Protean software. The base sequence encoding this peptide was amplified using PCR assay and ligated into the pET-28a vector, and the generated GRA24 truncated protein was transformed into <i>Escherichia coli</i> BL21. After induction by isopropyl-beta-D-thiogalactopyranoside (IPTG), the expression and purification of the recombinant GRA24 protein was analyzed using sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). BALB/c mice were immunized by subcutaneous injection with the purified recombinant GRA24 truncated protein to generate the polyclonal antibody, and the titer of the polyclonal antibody was measured using enzyme linked immunosorbent assay (ELISA). The specificity of the polyclonal antibody was tested using Western blotting, and the intracellular localization of the polyclonal antibody was investigated using immunofluorescence assay (IFA).</p><p><strong>Results: </strong>SDS-PAGE showed successful construction of the recombinant expression plasmid, and Coomassie brilliant blue staining showed the generation of the high-purity recombinant GRA24 truncated protein. ELISA measured that the titer of the polyclonal antibody against the GRA24 truncated protein was higher than 1:208 400, and Western blotting showed that the polyclonal antibody was effective to recognize the endogenous GRA24 proteins of different <i>T. gondii</i> strains and specifically recognize the recombinant GRA24 truncated protein. Indirect IFA showed that the GRA24 protein secreted 16 hour following <i>T. gondii</i> invasion in host cells.</p><p><strong>Conclusions: </strong>The polyclonal antibody against the <i>T. gondii</i> GRA24 protein has been successfully prepared, which has a widespread applicability, high titers and a high specificity. This polyclonal antibody is available for Western blotting and IFA, which provides the basis for investigating the function of the GRA24 protein.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"279-285"},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Overseas imported cystic echinococcosis misdiagnosed as pulmonary and hepatic cysts: a case report].","authors":"Z Huang, Y Li, S Gao, L Zhang, J Li, L Mi","doi":"10.16250/j.32.1374.2024059","DOIUrl":"10.16250/j.32.1374.2024059","url":null,"abstract":"<p><p>Cystic echinococcosis, a zoonotic disease that poses a significant threat to human health and animal husbandry development, is prevalent across the world and predominantly occurs in agricultural and pastoral regions. However, cystic echinococcosis cases are rare in non-endemic areas, which is likely to cause misdiagnosis or missing diagnosis, resulting in delay in treatment. This report presents an overseas imported cystic echinococcosis case misdiagnosed as pulmonary and hepatic cysts, so as to provide insights into diagnosis and treatment of cystic echinococcosis in non-endemic areas.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 4","pages":"435-438"},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preliminary observation on the development and dynamic changes of chronic toxoplasmosis in mice].","authors":"G Zhou, S Bai, Y Li, G Zhu, S Huang","doi":"10.16250/j.32.1374.2024044","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024044","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the development and dynamic changes of cysts in the brain of mice following infection with different forms of <i>Toxoplasma gondii</i>, so as to provide insights into for toxoplasmosis prevention and control.</p><p><strong>Methods: </strong>ICR mice at ages of 6 to 8 weeks, each weighing 20 to 25 g, were intraperitoneally injected with tachyzoites of the <i>T. gondii</i> PRU strain at a dose of 1 × 10⁵ tachyzoites per mouse, orally administered with cysts at a dose of 20 oocysts per mouse or oocysts at a dose of 200 oocysts per mouse for modeling chronic <i>T. gondii</i> infection in mice, and the clinical symptoms and survival of mice were observed post-infection. Mice were orally infected with <i>T. gondii</i> cysts at doses of 10 (low-dose group), 20 (medium-dose group), 40 cysts per mouse (high-dose group), and the effect of different doses of <i>T. gondii</i> infections on the number of cysts was examined in the mouse brain. Mice were orally administered with <i>T. gondii</i> cysts at a dose of 20 cysts per mouse, and grouped according to gender (female and male) and time points of infections (20, 30, 60, 90, 120, 150, 180 days post-infection), and the effects of gender and time points of infections on the number of cysts was examined in the mouse brain. In addition, mice were divided into the tachyzoite group (Group T), the first-generation cyst group (Group C1), the second-generation cyst group (Group C2), the third-generation cyst (Group C3) and the fourth-generation cyst group (Group C4). Mice in the Group T were intraperitoneally injected with <i>T. gondii</i> tachyzoites at a dose of 1 × 10⁵ tachyzoites per mouse, and the cysts were collected from the mouse brain tissues 30 days post-infection, while mice in the Group C1 were orally infected with the collected cysts at a dose of 30 cysts per mouse. Continuous passage was performed by oral administration with cysts produced by the previous generation in mice, and the effect of continuous passage on the number of cysts was examined in the mouse brain.</p><p><strong>Results: </strong>Following infection with <i>T. gondii</i> tachyzoites, cysts and oocysts in mice, obvious clinical symptoms were observed on days 6 to 13 and mice frequently died on days 7 to 12. The survival rates of mice were 67.0%, 87.0% and 53.0%, and the mean numbers of cysts were (516.0 ± 257.2), (1 203.0 ± 502.0) and (581.0 ± 183.1) in the mouse brain (<i>F</i> = 11.94, <i>P</i> < 0.01) on day 30 post-infection with <i>T. gondii</i> tachyzoites, cysts and oocysts, respectively, and the numbers of cysts in the brain tissues were significantly lower in mice infected with <i>T. gondii</i> tachyzoites and oocysts than in those infected with cysts (all <i>P</i> values < 0.01). The survival rates of mice were 87.0%, 87.0% and 60.0%, and the mean numbers of cysts were (953.0 ± 355.5), (1 084.0 ± 474.3) and (1 113.0 ± 546.0) in the mouse brain in the low-, me","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"304-309"},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}