Bioactive Carbohydrates and Dietary Fibre最新文献

{"title":"Enzymatic synthesis of analogous human milk oligosaccharide and its prebiotic attributes","authors":"Jayashree Niharika,&nbsp;Bhoomi Italiya,&nbsp;Ravindra Pal Singh","doi":"10.1016/j.bcdf.2025.100479","DOIUrl":"10.1016/j.bcdf.2025.100479","url":null,"abstract":"<div><div>Human milk oligosaccharides (HMOs) are the third most abundant component of human milk and deliver a myriad of benefits to infants including inhibiting the growth of pathogens and promoting the population of <em>Bifidobacterium</em>. In this study, glycoside hydrolase family 94 (GH94) member cellodextrin phosphorylase (CDP) has been used to synthesize a trisaccharide (Gal-β-1,4-Gal-β-1,4-Glc) in milligrams by transferring a galactose unit on cellobiose that mimics the primary building block of HMOs. Previous study of saturation transfer difference nuclear magnetic resonance spectroscopy indicated that the phosphate group of glucose-1-phosphate (Glc-1-P) makes hydrogen bonds with the side chain of H817, R486 and S889, and the backbone of G890, as well as a salt bridge with R486. We employed site-directed mutagenesis to determine the roles of these residues in synthesizing analogue HMO using galactose-1-phosphate (Gal-1-P). We did alanine scanning of these residues and found that mutation of R486A and G890A residues led to enzyme inactivation. Whilst mutation in H817A and S889A residues led to decreased enzymatic activity. These results further suggested that Gal-1-P bound at the donor site similar to a cognate donor (Glc-1-P). Using the native CDP, 40 mg of analogue HMO trisaccharide was produced with a 66 % yield. The product was well purified and analytically characterized. This trisaccharide is found to support the growth of probiotic bacteria such as <em>Bifidobacterium longum, Roseburia faecis,</em> and <em>Blautia producta</em>.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100479"},"PeriodicalIF":0.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"For cereal bran, rating of antioxidant capacity and screening of xanthine oxidase inhibitor","authors":"Ye Lou,&nbsp;Ming Cong Fan,&nbsp;Li Wang,&nbsp;Yan Li,&nbsp;Haifeng Qian","doi":"10.1016/j.bcdf.2025.100476","DOIUrl":"10.1016/j.bcdf.2025.100476","url":null,"abstract":"<div><div>Based on the free radical scavenging rate and xanthine oxidase (XOD) inhibition rate, the basic properties of different cereal bran were analyzed, and the key XOD inhibitor in bran extract was screened by ultrafiltration centrifugation combined with liquid chromatography. The effectiveness of ferulic acid (FA) in the regulation of hyperuricemia was evaluated by basic physiological indexes, expression of inflammatory factors and expression of urate transporter. The analysis of the basic properties of maize bran showed that the free phenol content of maize bran is 221.17 ± 8.03 mg GAE/100 g DW, and the bound phenol content is 245.88 ± 5.31 mg GAE/100 g DW, which is the highest among five kinds of cereal bran. The antioxidant capacity of bran was evaluated according to ABTS free radical scavenging rate and DPPH free radical scavenging rate. The IC₅₀ value of maize bran bound phenol was 0.23 μg/mL for ABTS and 2.76 μg/mL for DPPH. Eight potential XOD inhibitors were detected in maize bran bound phenol and among which FA had the highest XOD inhibition rate. Animal experiments demonstrated that FA could effectively alleviate slow weight gain, suppress the increased expression of inflammatory factors, and correct the abnormal expression of urate transporters in hyperuricemia-induced mice. The results can provide a basis that cereal bran as a dietary supplement and functional food take hyperuricemia regulation effect in the future.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100476"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aims and Scope & Editorial Board","authors":"","doi":"10.1016/S2212-6198(25)00011-7","DOIUrl":"10.1016/S2212-6198(25)00011-7","url":null,"abstract":"","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100477"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Characterization and antioxidant activity of flaxseed mucilage and evaluation of its dietary supplementation in improving calcium absorption in vivo” [Journal: Bioactive Carbohydrates and Dietary Fibre 32 (2024) 100444]","authors":"Engy M. Akl ,&nbsp;Rasha S. Mohamed ,&nbsp;Sherein S. Abdelgayed ,&nbsp;Karem Fouda ,&nbsp;Mosaad A. Abdel-Wahhab","doi":"10.1016/j.bcdf.2025.100471","DOIUrl":"10.1016/j.bcdf.2025.100471","url":null,"abstract":"","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100471"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The specificity of family 4 carbohydrate-binding modules (CBMs) of BpGH16MLG influences the binding and utilization of β-glucans in a human gut bacterium, Blautia producta","authors":"Harshita Gupta ,&nbsp;Chandani Gajera ,&nbsp;Prachi Kumari,&nbsp;Ravindra Pal Singh","doi":"10.1016/j.bcdf.2025.100475","DOIUrl":"10.1016/j.bcdf.2025.100475","url":null,"abstract":"<div><div>Several thousand bacterial communities reside in the human gut environment and have acquired numerous genes during co-evolution for trapping dietary nutrients. Many of Gram-positive bacteria encode extracellular cell wall membrane-bound endolytic enzymes with non-catalytic carbohydrate-binding modules (CBMs) that recognize cognate glycan(s). Much remains unknown about how these organisms recognize nutrients in the gut environment. <em>Blautia producta</em> JCM 1471^T expresses a cell membrane-tethered endo-acting enzyme (<em>Bp</em>GH16_MLG-digesting barley-β-glucan, lichenan, and laminarin) with four carbohydrate-binding modules (CBM4_1, CBM4_2, CBM4_3, and CBM4_4). Each CBM4 and its dual combinations were cloned, characterized, and assessed with three distinct β-glucans (barley-β-glucan, lichenan, and laminarin). It was observed that each CBM4 recognizes barley-β-glucan and lichenan with approximately equal affinity. Dual combination, CBM4_1-CBM4_2 exhibited a substantial predilection for barley-β-glucan, whereas CBM4_3-CBM4_4 had an affinity for barley-β-glucan and lichenan. None of the dual combinations showed an affinity for laminarin. Intriguingly, a mutant (E384A-catalytic base) version of <em>Bp</em>GH16_MLG with all CBM4 domains showed high binding to barley-β-glucans and lichenan, but moderate binding to laminarin. This understanding provides insights into how <em>B. producta</em> recognizes and likely traps specific nutrient in the gut environment. Furthermore, the study may have implications for the development of innovative biocatalysis.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100475"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring potential of aloe vera and sterculia gum dietary fibers to develop functional hydrogels for drug delivery applications","authors":"Diwanshi Sharma,&nbsp;Baljit Singh","doi":"10.1016/j.bcdf.2025.100474","DOIUrl":"10.1016/j.bcdf.2025.100474","url":null,"abstract":"<div><div>Now days the main focus of carbohydrate research is to design functional hydrogels for biomedical applications. The aloe vera (AV) and sterculia gum (SG) are therapeutically active dietary fibers. Herein, these polysaccharides were explored to develop network hydrogels by covalent and non-covalent interactions for use in drug delivery (DD) and wound dressing (WD) applications. These hydrogel dressings were impregnated with antibiotic drug moxifloxacin for better wound care. Slow and sustained diffusion of drug was found with a non-Fickian mechanism and release profile was best explained by Higuchi kinetic model. Additionally, various physiological and biomedical properties of hydrogels were also evaluated. The dressings exhibited an impressive wound fluid absorption tendency under simulated conditions. The network structure demonstrated mucoadhesive properties and required 97 ± 6.00 mN forces for the detachment of dressings from mucosal surface. Dressing revealed significant antioxidant activity which demonstrated 49.14 ± 0.32 % free radical scavenging in DPPH assay. The dressings were non-toxic and were permeable to O₂ &amp; H₂O. The characterization of hydrogel dressings was performed through Field emission scanning electron microscopy/Energy dispersive spectroscopy (FESEM/EDS), Atomic force microscopy (AFM), ¹³Carbon Nuclear magnetic resonance spectroscopy (¹³C NMR), Fourier transform infrared spectroscopy (FTIR) &amp; X-ray diffraction (XRD) techniques to establish the structural aspects of copolymers. Overall, physicochemical &amp; biochemical findings suggested that these hydrogels could be applied in DD applications. These hydrogels developed from bioactive AV and SG could be applied as wound dressings encapsulated with antibiotic drug for better wound healing.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100474"},"PeriodicalIF":0.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary prebiotic combination improves immunological and hematological parameters in weaned pigs","authors":"Patrícia Versuti Arantes Alvarenga ,&nbsp;Vinicius Ricardo Cambito de Paula ,&nbsp;Mayra Anton Dib Saleh ,&nbsp;Silvia Letícia Ferreira ,&nbsp;Filipe Garcia Telles ,&nbsp;Ricardo de Oliveira Orsi ,&nbsp;Carlos Roberto Padovani ,&nbsp;Urbano dos Santos Ruiz ,&nbsp;Marcos Livio Panhoza Tse","doi":"10.1016/j.bcdf.2025.100473","DOIUrl":"10.1016/j.bcdf.2025.100473","url":null,"abstract":"<div><div>As antimicrobial resistance is a global public health problem and as known the use of prebiotics on animal nutrition have antimicrobial effects, we hypothesized the use of prebiotic additives can be an alternative to in-feed antibiotics for piglets to control sanitary challenge. Thus, the aim of this study was to evaluate the inclusion of prebiotics mannan<strong>-</strong>oligosaccharide (MOS), β-glucan, fructooligosaccharide (FOS) and galacto-oligosaccharides (GOS) to replace antibiotics as growth promoters, on immunological and hematological parameters of 21d-old weaned pigs challenged with intramuscular injection of 30 μg kg⁻¹ body weight of <em>Escherichia coli</em> lipopolysaccharide (LPS). Forty weaned piglets (7.2 kg) fed: CON = basal diet (BD) plus 120 ppm of halquinol; Mβ = BD plus a blend of MOS and β-glucan; F₉G₁ = BD plus a blend of MOS and β-glucan and a blend of FOS/GOS (ratio 9:1); F₇G3 = BD plus a blend of MOS and β-glucan and a blend of FOS/GOS (ratio 7:3); F₅G₅ = BD plus a blend of MOS and β-glucan and a blend of FOS/GOS (ratio 5:5). After LPS challenge, the animals fed F₉G₁ had higher hydrogen peroxide (H₂O₂) and the same nitric oxide (NO) serum concentration than CON. Animals fed Mβ and F₉G₁ showed similar serum C-reactive protein (CP) concentration than CON and serum cytokine tumor necrosis factor alpha (TNF-α) concentration were higher in animals fed F₉G₁ or F₅G₅ than CON. In general, we concluded that treatment F₉G₁, is better for immune response during immunological challenge, because more effective immunological variables were affected (H₂O₂, NO, CP and TNF-α).</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100473"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic modification of wheat bran: Effect on functional and physicochemical properties","authors":"Reshma Saroj ,&nbsp;Mudasir Ahmad Malik ,&nbsp;Devinder Kaur","doi":"10.1016/j.bcdf.2025.100472","DOIUrl":"10.1016/j.bcdf.2025.100472","url":null,"abstract":"<div><div>Bran from six various wheat varieties was used in this study subjected to enzymatic treatment and was evaluated for physicochemical, functional and antioxidant properties along with mineral content analysis. Six wheat cultivars used for the study were HUW-234, PBW-373, WH-1105, PBW-502, HD-2967, and PBW-343. Wheat bran was treated with three different enzymes namely β-gluconase, Xylanase and Cellulase. The functional properties of bran were substantially altered by the enzyme treatment. All enzyme treatments caused increase in bulk density and decrease in water binding capacity, oil binding capacity and swelling power. Maximum effect on functional properties was observed by the cellulase enzyme modification. Total phenol content and antioxidant properties were increased after enzyme treatment and maximum increase was found in cellulase enzyme treatment followed by xylanase and β-gluconase. Soluble fiber content was increased while as insoluble fiber content was decrease after enzyme modification. The concentration of calcium was found highest and concentration of cupper was found lowest in all bran samples and concentration of all the studied minerals increased significantly (p ≤ 0.05) after enzyme treatment. Pearson correlation and he principal component analysis was carried out to determine the relationship among different physicochemical, functional and antioxidant properties of bran samples.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100472"},"PeriodicalIF":0.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor activity of polysaccharides obtained from guavira fruit industrial waste on murine melanoma cells","authors":"Rafaella Fernanda Angeli ,&nbsp;Amanda Plaça Bialli ,&nbsp;Suelen Cristina Soares Baal ,&nbsp;Elisvânia Freitas dos Santos ,&nbsp;Paulo Sérgio Loubet Filho ,&nbsp;Vanessa Suzane Schneider ,&nbsp;Luiz Cláudio Fernandes ,&nbsp;Katya Naliwaiko ,&nbsp;Lucimara Mach Côrtes Cordeiro ,&nbsp;Fabíola Iagher","doi":"10.1016/j.bcdf.2025.100469","DOIUrl":"10.1016/j.bcdf.2025.100469","url":null,"abstract":"<div><div>Melanoma is an aggressive type of cancer with increasing incidence worldwide. Chemotherapy drugs are associated with several side effects that lead to lost quality of life for patients undergoing treatment. This fact has encouraged the search for compounds capable of acting as adjuvants in cancer treatment with fewer side effects. Pectins are soluble polysaccharides with diverse molecular structures and important biological properties, which can be obtained from fruit parts often discarded by the food industry. The present study investigated the antitumor effect of pectins present in guavira pomace flour (<em>Campomanesia adamantium</em> and <em>C. pubescens</em>) on B16F10 cells. The assays evaluated viability, cell death, proliferation rate, migratory ability, and potential for colony formation. The results showed that pectins (CPW fraction) at concentrations of 5 and 100 μg/mL reduced the viability of tumor cells (∼20–40%, respectively, when compared to the untreated group), with less cytotoxicity on non-tumor cells (BALB/c 3T3) than the chemotherapy drug Doxorubicin (p &lt; 0.05). Similarly, the proliferation rate was decreased in B16F10 cells in the presence of two CPW concentrations (∼33 and 40%, respectively). In the flow cytometry assay, the CPW treatment did not increase the number of dead cells, suggesting cell cycle arrest. In addition, the CPW fraction reduced the migratory capacity of tumor cells (∼8 and 12%, respectively) and the number of new colonies formed (∼30 and 70%, respectively). Taken together, these results suggest that the pectins of guavira pomace flour, a discarded industrial material, have important antitumor activity with adjuvant therapeutic potential for cancer treatment.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100469"},"PeriodicalIF":0.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143296550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of sulfated chitosan from cuttlebone of Sepia pharaonis (Ehrenberg, 1831) and its capacity to inhibit blood clotting","authors":"Hirshasri Ambalarajan Gunasekaran ,&nbsp;Annathai Pitchai ,&nbsp;Pasiyappazham Ramasamy","doi":"10.1016/j.bcdf.2025.100470","DOIUrl":"10.1016/j.bcdf.2025.100470","url":null,"abstract":"<div><div>The synthesis of sulfated chitosan from the cuttlebone of <em>Sepia pharaonis</em> (Ehrenberg, 1831) and its potential to inhibit blood clotting were investigated in this study. Cuttlebone-derived chitosan was chemically modified by sulfation to produce sulfated chitosan. This modification was achieved by introducing sulfate groups into the chitosan structure, thereby enhancing its biological properties. The synthesized sulfated chitosan demonstrated a significant capacity to inhibit blood clotting, indicating its potential application as an anticoagulant. Anticoagulant activity was evaluated through <em>in vitro</em> assays, where sulfated chitosan exhibited notable suppression of clot formation. Moreover, this study examined the structural and physicochemical properties (FTIR, XRD, FESEM) of the synthesized sulfated chitosan, providing insights into its potential mechanism of action. These findings suggest that sulfated chitosan derived from <em>Sepia pharaonis</em> cuttlebone holds promise as an effective and biocompatible anticoagulant, thereby presenting a potential avenue for the development of novel therapeutic interventions in the field of cardiovascular medicine and related disciplines.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100470"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}