Impact of enzymatically derived manno-oligosaccharides on the growth of select lactobacillus strains under aerobic and anaerobic conditions

Abstract

This study highlights the ability of beta-MOS derived from coconut processing residues (copra meal) to promote the in vitro growth of Lactobacillus probiotics. Enzymatic hydrolysis conditions were manipulated to produce Low Molecular Weight (LMW) and (HMW) Manno-oligosaccharide (MOS) extracts. The LMW MOS contained more mannose and mannobiose, and the HMW MOS contained more mannotriose and higher molecular weight oligosaccharides. The growth of Limosilactobacillus reuteri, Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus GG on the MOS Extracts was evaluated and compared with growth on Inulin, FOS and XOS. There was significant growth of each probiotic on the MOS extracts. Probiotic growth was similar on MOS extracts and on FOS, while growth was much lower on XOS and Inulin. Probiotic growth was greater under anaerobic than under aerobic conditions. The results confirm the value of a bioactive compound from copra meal to support human and animal health, while contributing to a circular bioeconomy.