Bioactive Carbohydrates and Dietary Fibre最新文献

{"title":"The specificity of family 4 carbohydrate-binding modules (CBMs) of BpGH16MLG influences the binding and utilization of β-glucans in a human gut bacterium, Blautia producta","authors":"Harshita Gupta ,&nbsp;Chandani Gajera ,&nbsp;Prachi Kumari,&nbsp;Ravindra Pal Singh","doi":"10.1016/j.bcdf.2025.100475","DOIUrl":"10.1016/j.bcdf.2025.100475","url":null,"abstract":"<div><div>Several thousand bacterial communities reside in the human gut environment and have acquired numerous genes during co-evolution for trapping dietary nutrients. Many of Gram-positive bacteria encode extracellular cell wall membrane-bound endolytic enzymes with non-catalytic carbohydrate-binding modules (CBMs) that recognize cognate glycan(s). Much remains unknown about how these organisms recognize nutrients in the gut environment. <em>Blautia producta</em> JCM 1471^T expresses a cell membrane-tethered endo-acting enzyme (<em>Bp</em>GH16_MLG-digesting barley-β-glucan, lichenan, and laminarin) with four carbohydrate-binding modules (CBM4_1, CBM4_2, CBM4_3, and CBM4_4). Each CBM4 and its dual combinations were cloned, characterized, and assessed with three distinct β-glucans (barley-β-glucan, lichenan, and laminarin). It was observed that each CBM4 recognizes barley-β-glucan and lichenan with approximately equal affinity. Dual combination, CBM4_1-CBM4_2 exhibited a substantial predilection for barley-β-glucan, whereas CBM4_3-CBM4_4 had an affinity for barley-β-glucan and lichenan. None of the dual combinations showed an affinity for laminarin. Intriguingly, a mutant (E384A-catalytic base) version of <em>Bp</em>GH16_MLG with all CBM4 domains showed high binding to barley-β-glucans and lichenan, but moderate binding to laminarin. This understanding provides insights into how <em>B. producta</em> recognizes and likely traps specific nutrient in the gut environment. Furthermore, the study may have implications for the development of innovative biocatalysis.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100475"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring potential of aloe vera and sterculia gum dietary fibers to develop functional hydrogels for drug delivery applications","authors":"Diwanshi Sharma,&nbsp;Baljit Singh","doi":"10.1016/j.bcdf.2025.100474","DOIUrl":"10.1016/j.bcdf.2025.100474","url":null,"abstract":"<div><div>Now days the main focus of carbohydrate research is to design functional hydrogels for biomedical applications. The aloe vera (AV) and sterculia gum (SG) are therapeutically active dietary fibers. Herein, these polysaccharides were explored to develop network hydrogels by covalent and non-covalent interactions for use in drug delivery (DD) and wound dressing (WD) applications. These hydrogel dressings were impregnated with antibiotic drug moxifloxacin for better wound care. Slow and sustained diffusion of drug was found with a non-Fickian mechanism and release profile was best explained by Higuchi kinetic model. Additionally, various physiological and biomedical properties of hydrogels were also evaluated. The dressings exhibited an impressive wound fluid absorption tendency under simulated conditions. The network structure demonstrated mucoadhesive properties and required 97 ± 6.00 mN forces for the detachment of dressings from mucosal surface. Dressing revealed significant antioxidant activity which demonstrated 49.14 ± 0.32 % free radical scavenging in DPPH assay. The dressings were non-toxic and were permeable to O₂ &amp; H₂O. The characterization of hydrogel dressings was performed through Field emission scanning electron microscopy/Energy dispersive spectroscopy (FESEM/EDS), Atomic force microscopy (AFM), ¹³Carbon Nuclear magnetic resonance spectroscopy (¹³C NMR), Fourier transform infrared spectroscopy (FTIR) &amp; X-ray diffraction (XRD) techniques to establish the structural aspects of copolymers. Overall, physicochemical &amp; biochemical findings suggested that these hydrogels could be applied in DD applications. These hydrogels developed from bioactive AV and SG could be applied as wound dressings encapsulated with antibiotic drug for better wound healing.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100474"},"PeriodicalIF":0.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary prebiotic combination improves immunological and hematological parameters in weaned pigs","authors":"Patrícia Versuti Arantes Alvarenga ,&nbsp;Vinicius Ricardo Cambito de Paula ,&nbsp;Mayra Anton Dib Saleh ,&nbsp;Silvia Letícia Ferreira ,&nbsp;Filipe Garcia Telles ,&nbsp;Ricardo de Oliveira Orsi ,&nbsp;Carlos Roberto Padovani ,&nbsp;Urbano dos Santos Ruiz ,&nbsp;Marcos Livio Panhoza Tse","doi":"10.1016/j.bcdf.2025.100473","DOIUrl":"10.1016/j.bcdf.2025.100473","url":null,"abstract":"<div><div>As antimicrobial resistance is a global public health problem and as known the use of prebiotics on animal nutrition have antimicrobial effects, we hypothesized the use of prebiotic additives can be an alternative to in-feed antibiotics for piglets to control sanitary challenge. Thus, the aim of this study was to evaluate the inclusion of prebiotics mannan<strong>-</strong>oligosaccharide (MOS), β-glucan, fructooligosaccharide (FOS) and galacto-oligosaccharides (GOS) to replace antibiotics as growth promoters, on immunological and hematological parameters of 21d-old weaned pigs challenged with intramuscular injection of 30 μg kg⁻¹ body weight of <em>Escherichia coli</em> lipopolysaccharide (LPS). Forty weaned piglets (7.2 kg) fed: CON = basal diet (BD) plus 120 ppm of halquinol; Mβ = BD plus a blend of MOS and β-glucan; F₉G₁ = BD plus a blend of MOS and β-glucan and a blend of FOS/GOS (ratio 9:1); F₇G3 = BD plus a blend of MOS and β-glucan and a blend of FOS/GOS (ratio 7:3); F₅G₅ = BD plus a blend of MOS and β-glucan and a blend of FOS/GOS (ratio 5:5). After LPS challenge, the animals fed F₉G₁ had higher hydrogen peroxide (H₂O₂) and the same nitric oxide (NO) serum concentration than CON. Animals fed Mβ and F₉G₁ showed similar serum C-reactive protein (CP) concentration than CON and serum cytokine tumor necrosis factor alpha (TNF-α) concentration were higher in animals fed F₉G₁ or F₅G₅ than CON. In general, we concluded that treatment F₉G₁, is better for immune response during immunological challenge, because more effective immunological variables were affected (H₂O₂, NO, CP and TNF-α).</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100473"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic modification of wheat bran: Effect on functional and physicochemical properties","authors":"Reshma Saroj ,&nbsp;Mudasir Ahmad Malik ,&nbsp;Devinder Kaur","doi":"10.1016/j.bcdf.2025.100472","DOIUrl":"10.1016/j.bcdf.2025.100472","url":null,"abstract":"<div><div>Bran from six various wheat varieties was used in this study subjected to enzymatic treatment and was evaluated for physicochemical, functional and antioxidant properties along with mineral content analysis. Six wheat cultivars used for the study were HUW-234, PBW-373, WH-1105, PBW-502, HD-2967, and PBW-343. Wheat bran was treated with three different enzymes namely β-gluconase, Xylanase and Cellulase. The functional properties of bran were substantially altered by the enzyme treatment. All enzyme treatments caused increase in bulk density and decrease in water binding capacity, oil binding capacity and swelling power. Maximum effect on functional properties was observed by the cellulase enzyme modification. Total phenol content and antioxidant properties were increased after enzyme treatment and maximum increase was found in cellulase enzyme treatment followed by xylanase and β-gluconase. Soluble fiber content was increased while as insoluble fiber content was decrease after enzyme modification. The concentration of calcium was found highest and concentration of cupper was found lowest in all bran samples and concentration of all the studied minerals increased significantly (p ≤ 0.05) after enzyme treatment. Pearson correlation and he principal component analysis was carried out to determine the relationship among different physicochemical, functional and antioxidant properties of bran samples.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100472"},"PeriodicalIF":0.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor activity of polysaccharides obtained from guavira fruit industrial waste on murine melanoma cells","authors":"Rafaella Fernanda Angeli ,&nbsp;Amanda Plaça Bialli ,&nbsp;Suelen Cristina Soares Baal ,&nbsp;Elisvânia Freitas dos Santos ,&nbsp;Paulo Sérgio Loubet Filho ,&nbsp;Vanessa Suzane Schneider ,&nbsp;Luiz Cláudio Fernandes ,&nbsp;Katya Naliwaiko ,&nbsp;Lucimara Mach Côrtes Cordeiro ,&nbsp;Fabíola Iagher","doi":"10.1016/j.bcdf.2025.100469","DOIUrl":"10.1016/j.bcdf.2025.100469","url":null,"abstract":"<div><div>Melanoma is an aggressive type of cancer with increasing incidence worldwide. Chemotherapy drugs are associated with several side effects that lead to lost quality of life for patients undergoing treatment. This fact has encouraged the search for compounds capable of acting as adjuvants in cancer treatment with fewer side effects. Pectins are soluble polysaccharides with diverse molecular structures and important biological properties, which can be obtained from fruit parts often discarded by the food industry. The present study investigated the antitumor effect of pectins present in guavira pomace flour (<em>Campomanesia adamantium</em> and <em>C. pubescens</em>) on B16F10 cells. The assays evaluated viability, cell death, proliferation rate, migratory ability, and potential for colony formation. The results showed that pectins (CPW fraction) at concentrations of 5 and 100 μg/mL reduced the viability of tumor cells (∼20–40%, respectively, when compared to the untreated group), with less cytotoxicity on non-tumor cells (BALB/c 3T3) than the chemotherapy drug Doxorubicin (p &lt; 0.05). Similarly, the proliferation rate was decreased in B16F10 cells in the presence of two CPW concentrations (∼33 and 40%, respectively). In the flow cytometry assay, the CPW treatment did not increase the number of dead cells, suggesting cell cycle arrest. In addition, the CPW fraction reduced the migratory capacity of tumor cells (∼8 and 12%, respectively) and the number of new colonies formed (∼30 and 70%, respectively). Taken together, these results suggest that the pectins of guavira pomace flour, a discarded industrial material, have important antitumor activity with adjuvant therapeutic potential for cancer treatment.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100469"},"PeriodicalIF":0.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143296550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of sulfated chitosan from cuttlebone of Sepia pharaonis (Ehrenberg, 1831) and its capacity to inhibit blood clotting","authors":"Hirshasri Ambalarajan Gunasekaran ,&nbsp;Annathai Pitchai ,&nbsp;Pasiyappazham Ramasamy","doi":"10.1016/j.bcdf.2025.100470","DOIUrl":"10.1016/j.bcdf.2025.100470","url":null,"abstract":"<div><div>The synthesis of sulfated chitosan from the cuttlebone of <em>Sepia pharaonis</em> (Ehrenberg, 1831) and its potential to inhibit blood clotting were investigated in this study. Cuttlebone-derived chitosan was chemically modified by sulfation to produce sulfated chitosan. This modification was achieved by introducing sulfate groups into the chitosan structure, thereby enhancing its biological properties. The synthesized sulfated chitosan demonstrated a significant capacity to inhibit blood clotting, indicating its potential application as an anticoagulant. Anticoagulant activity was evaluated through <em>in vitro</em> assays, where sulfated chitosan exhibited notable suppression of clot formation. Moreover, this study examined the structural and physicochemical properties (FTIR, XRD, FESEM) of the synthesized sulfated chitosan, providing insights into its potential mechanism of action. These findings suggest that sulfated chitosan derived from <em>Sepia pharaonis</em> cuttlebone holds promise as an effective and biocompatible anticoagulant, thereby presenting a potential avenue for the development of novel therapeutic interventions in the field of cardiovascular medicine and related disciplines.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100470"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Livaux® gold kiwifruit powder consumption at 600 mg per day for 28 days increases Faecalibacterium prausnitzii numbers and decreases bloating and hydrogenotrophic species numbers in healthy individuals, consistent with slow fermentation: A randomized controlled trial","authors":"Starin McKeen ,&nbsp;Susanne M. Henning ,&nbsp;Erin Lewis ,&nbsp;Malkanthi Evans ,&nbsp;Emma Graham ,&nbsp;Neville Jopson ,&nbsp;Jennifer Gu ,&nbsp;Doug Rosendale","doi":"10.1016/j.bcdf.2025.100468","DOIUrl":"10.1016/j.bcdf.2025.100468","url":null,"abstract":"<div><div>Kiwifruit is renowned for its benefits to laxation. Livaux® is a gold kiwifruit powder retaining whole fruit benefits, including high-methoxy pectin content. Livaux was previously shown to provide laxation support and increased faecal <em>F. prausnitzii</em>. <em>F. prausnitzii</em> is known to use high-methoxy pectin as substrate.</div><div>We aimed to determine the changes to laxation and faecal <em>F. prausnitzii</em> numbers from 600 mg of Livaux. We used a randomized, double-blinded, placebo-controlled parallel-arm study with 85 participants conducted across four North American sites, comparing 28 days of 600 mg Livaux consumption vs cellulose placebo. The study was powered for the primary outcome Complete Spontaneous Bowel Movements (CSBM).</div><div>Participants visited at screening, baseline and endpoint. Anthropometric measures, hematology, clinical chemistry and hematology and fecal samples for microbiome assessment were collected at baseline and endpoint. Clinical outcomes (CSBM, Bristol Stool Form Score (BSFS), spontaneous bowel movements (SBM) and participant-assessed constipation symptoms and quality of life), food diaries and adverse events were recorded daily throughout the study. Analysis of the intent-to-treat population assessed differences from baseline to endpoint using ANOVA with post-hoc Tukey's T-test, an assessment of change from baseline to days 7, 14, 21 and 28 using repeated measures ANCOVA.</div><div>Livaux supplemented participants showed significantly increased fecal <em>F. prausnitzii</em> relative abundance, whilst placebo did not. Participants also showed significantly improved CSBM, SBM, BSFS and constipation symptom and quality of life scores over baseline.</div><div>Livaux supplementation improved laxation and increased <em>F. prausnitzii</em> at the low dose of 600 mg.</div><div>This study was registered at <span><span>https://clinicaltrials.gov/study/NCT03462199</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100468"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical characterization of starch from Maranta arundinacea L. (arrowroot) rhizomes and its inhibition of COX-2: In vivo validation","authors":"Nishaa Surendran ,&nbsp;Manikandan Vani Raju ,&nbsp;Meenakshi Kaniyur Chandrasekaran ,&nbsp;Rathi Muthaiyan Ahalliya ,&nbsp;Chella Perumal Palanisamy ,&nbsp;Geethadevi Chandrasekaran ,&nbsp;Gopalakrishnan Velliyur Kanniappan","doi":"10.1016/j.bcdf.2024.100465","DOIUrl":"10.1016/j.bcdf.2024.100465","url":null,"abstract":"<div><div><em>Maranta arundinacea</em> L. (<em>M. arundinacea</em> L.) that belongs to the family Marantaceae is an underutilized tuber. The starch from the rhizomatous tubers is highly commercial and medicinal value is uncharacterized. This study was aimed at isolating the starch from <em>Maranta arundinacea</em> L. rhizomes, analyzing its physiochemical properties and examine its influence on anti-inflammatory activity. The physicochemical properties like Moisture and ash content, water holding capacity, water absorption index, amylose content, light transmittance and structural morphology of starch granules. Anti-inflammatory activity of starch from <em>M. arundinacea</em> L. was evaluated in Wistar rats by carrageenan induced paw edema. The biochemical estimation of serum enzymes, lipid peroxidation, enzymatic antioxidants and histopathological studies of paw tissues were carried out in paw tissues. Molecular expression of proinflammatory COX-2 gene was also analyzed. <em>M. arundinacea</em> starch was found to contain 9.3% moisture, 1.36% ash and 26.3% amylose content. The light transmittance (%) decreased with an increase in time. Micrographs of SEM analysis showed variations in the shape of granules that appeared as spherical and irregular. The size of granules varied between 2.86 and 6.92 μm. Attenuation in paw edema on treatment with <em>M. arundinacea</em> L. starch (200 mg/kg) was observed in comparison with that of standard drug Diclofenac sodium, the serum enzymes and enzymatic antioxidants were brought to near normal. The potent anti-inflammatory activities were confirmed by Histopathological studies. Molecular expression of proinflammatory COX-2 gene was also down regulated. It can be concluded that the dietary supplementation of starch from <em>M. arundinacea</em> L. to mitigate inflammation and reduce chronic diseases.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100465"},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sorghum cookies fortified with Garcinia mangostana peel extract: Formulation, characterization, and evaluation of antioxidant and antidiabetic activity","authors":"Anastasia Wheni Indrianingsih ,&nbsp;Yuniar Khasanah ,&nbsp;Cici Darsih ,&nbsp;Heru Pitria Hastuti ,&nbsp;Ade Erma Suryani ,&nbsp;Margi Hastuti ,&nbsp;Dwi Ni'maturrohmah ,&nbsp;Umi Laila ,&nbsp;Eka Noviana ,&nbsp;Eka Rahayu ,&nbsp;Tri Wiyono","doi":"10.1016/j.bcdf.2025.100467","DOIUrl":"10.1016/j.bcdf.2025.100467","url":null,"abstract":"<div><div>Increased consumption of processed food and sugary beverages is a major contributor to chronic diseases such as diabetes mellitus and cardiovascular diseases. This research aimed to develop sorghum-based cookie fortified with mangosteen (<em>Garcinia mangostana</em>) peel extract, as a healthy, yet convenient food alternative. Both sorghum and mangosteen are rich in phenolic compounds that have been shown to possess several biological activities including antioxidant and antidiabetic activities. The cookies were evaluated physically and chemically by performing sensory, color, texture, proximate, and chemical content analysis, determination of total phenolic content, and further characterizations using scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), and Fourier transform infrared spectroscopy (FTIR). The antioxidant and antidiabetic potencies of the cookies were assessed using radical scavenging and α-glucosidase inhibitory assays, respectively. The results showed that substituting wheat with sorghum flour and adding mangosteen peel extract to the cookie ingredients increased its antioxidant and antidiabetic activity. Several chemical compounds detected from the sorghum cookies included xanthones, phenolics, flavonoids, and alkaloids. The mangosteen peel extract-fortified sorghum cookies were well accepted by the panelists based on sensory and hedonic testing. Hence, sorghum flour and mangosteen peel can be used as excellent ingredients for making healthy cookies.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100467"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guar galactomannan ameliorates radiation-induced intestinal injury in mice","authors":"Darshini Shivamogga Mohan ,&nbsp;Murali Badanthadka ,&nbsp;Anushri Umesh ,&nbsp;Bharath Basavapattana Rudresh ,&nbsp;Manjunatha Bukkambudhi Krishnaswamy ,&nbsp;Rashmi Kanugodu Vasappa ,&nbsp;Sahayog Narayan Jamdar ,&nbsp;Vidya Shimoga Muddappa","doi":"10.1016/j.bcdf.2024.100466","DOIUrl":"10.1016/j.bcdf.2024.100466","url":null,"abstract":"<div><div>Guar galactomannan (GGM), a widely used hydrocolloid in the food industry, was evaluated for its biological activity with respect to radioprotection. In an <em>in vitro</em> lymphocyte proliferation study, GGM showed significant proliferation of blood lymphocytes at a concentration of 50 μg/ml. The survival study of 30 days showed that GGM pre-treatment improves the survival in irradiated (7.5 Gy) mice to 70% which otherwise is just 30% in the control irradiated group. On day 3, the WBC count was maintained in the GGM pretreated IR mice group compared to the irradiated control group. Serum enzyme levels in the liver of the GGM pretreated irradiated group were reduced, spleen and thymus weight indices and antioxidant enzymes were increased in the intestine of the GGM pretreated irradiated group compared to the irradiated group. Histopathological studies of ileum and jejunum on day 3 revealed that there was less damage in the crypt and height of villi in the GGM pretreated IR mice group compared to the irradiated control group. In the differential gene expression of jejunum, a total of 43 genes showed differential expressions, suggesting their involvement in the biological response to the effect of galactomannan treatment on irradiated mice. Differential expression of genes shows that upregulation of the genes is related to limiting the damage to the intestine and stem cell genes involved in cell differentiation and function. Moreover, genes associated with apoptosis and inflammation were shown to be downregulated in the GGM-pretreated IR group. These results suggest that GGM improves mice from radiation-induced injury by increasing WBC count, reducing acute liver damage, maintaining spleen and thymus weight, increasing the levels of antioxidant enzymes and altering gene expression.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100466"},"PeriodicalIF":0.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}