Bioactive Carbohydrates and Dietary Fibre最新文献

{"title":"Prebiotic effects and functional insights into gut microbiota mediated by Qingke β-glucan of different molecular weights in vitro fermentation","authors":"Lan Zhao ,&nbsp;Xinran Yang ,&nbsp;Peijie Ye ,&nbsp;Zhihai Lan ,&nbsp;Jiale Cheng ,&nbsp;Li Li ,&nbsp;Jia Wu ,&nbsp;Wei Xu ,&nbsp;Huibin Chen","doi":"10.1016/j.bcdf.2025.100488","DOIUrl":"10.1016/j.bcdf.2025.100488","url":null,"abstract":"<div><div>Qingke β-glucan (QBG), a gastrointestinal indigestible grain polysaccharide, is used as prebiotic and delivery carrier to mediate gut microbiota, while the mechanism of QBG of different MWs degraded by the gut microbiota remain uncertain. In this study, we investigated the impacts of QBG with different MWs on gut microbiota, SCFAs, and the degrading degree of polysaccharides <em>in vitro</em> fermentation. QBG groups significantly increased the total SCFAs, which is consistent with the results predicted by PICRUSt2 based on the KEGG pathway. The 27 functional genes associated with SCFAs metabolism were identified. Acetate and propionate synthesis was primarily mediated by the <em>pta, ackA</em>, and <em>ACSS</em> genes, whereas butyrate production mediated by the <em>ptb, buk</em>, and <em>atoA</em> genes. The low-MW QBG produced more SCFAs than the high-MW QBG after fermentation, suggesting that polysaccharide MW influences its biological functionality. These findings support polysaccharide-based gut microbiota-targeting drug and food development.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100488"},"PeriodicalIF":3.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pectinase immobilized by encapsulation and entrapment and their effect on papaya juice clarification","authors":"Laishram Bhaktaraj Singh,&nbsp;Bhaskar Jyoti Kalita,&nbsp;Nandan Sit","doi":"10.1016/j.bcdf.2025.100489","DOIUrl":"10.1016/j.bcdf.2025.100489","url":null,"abstract":"<div><div>The present work explores the immobilization of pectinase through two methods: encapsulation in calcium alginate beads and matrix entrapment in agar cubes and evaluates their effectiveness in clarifying papaya juice compared to free pectinase. An immobilization efficiency of 72.59 ± 2.41 % for 2 % sodium alginate and 91.28 ± 3.62 % for 2 % agar were found to be the best. Optimal enzymatic activity for free pectinase was obtained at 40 °C and pH 6.0, and for both immobilized pectinase at 50 °C. The optimal pH was pH 8.0 for calcium alginate bead-encapsulated pectinase and pH 4.0 for agar cube-entrapped pectinase. The kinetic parameters for free pectinase, encapsulated pectinase and entrapped pectinase were K_m of 25.11 mg/mL, 16.29 mg/mL and 16.00 mg/mL and V_max of 4.57 μmol/mL/min, 2.79 μmol/mL/min and 1.92 μmol/mL/min respectively. The encapsulated pectinase and entrapped pectinase were reused for 5 cycles with 50.55 % and 80.03 % residual activity and remained stable for 15 days. In the application for papaya juice clarification tests, agar-entrapped pectinase significantly improved optical density and reduced viscosity, achieving the best results among the tested pectinase preparations.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100489"},"PeriodicalIF":3.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical characterization and anticancer evaluation of Indian traditional rice Cultivars: Insights into their therapeutic potential","authors":"M. Udayamathi ,&nbsp;P. Mohanapriya ,&nbsp;S. Mahalakshmi ,&nbsp;T. Subashini ,&nbsp;S. Divya Sri ,&nbsp;S. Pramila ,&nbsp;A. Saravana Raj ,&nbsp;Yuvaraj Dinakarkumar","doi":"10.1016/j.bcdf.2025.100487","DOIUrl":"10.1016/j.bcdf.2025.100487","url":null,"abstract":"<div><div>This study evaluates the phytochemical, antioxidant, and anticancer properties of three traditional rice varieties from Tamil Nadu, India: Thuyamalli, Kuruvikar, and Kudaivazhai. Gas chromatography-mass spectrometry (GC-MS) identified 15 bioactive compounds, with 9-octadecenoic acid methyl ester showing the highest intensity (32.11 %). Fourier transform infrared (FTIR) spectroscopy revealed functional groups like alcohols and alkanes in all varieties. Antioxidant activity measured through DPPH assay demonstrated Kudaivazhai rice's highest scavenging activity (EC50 = 220 μL), followed by Kuruvikar (EC50 = 192 μL) and Thuyamalli (EC50 = 170 μL). Molecular docking studies indicated strong interactions between rice-derived compounds and breast cancer proteins, with 9-octadecenoic acid methyl ester achieving a docking score of −11.13 kcal/mol. These findings highlight the high phenolic content, flavonoid levels, and antioxidant activity of these rice varieties, supporting their potential as anticancer agents. This study emphasizes the therapeutic and nutritional value of traditional rice, advocating their conservation and dietary inclusion.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100487"},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, purification and structural characterization of inulin-type fructans from different selected Asparagus species","authors":"Deshani C. Mudannayake ,&nbsp;Wasantha K. Meegahawaththa ,&nbsp;Ashinshana U. Illippangama ,&nbsp;Herath M.J.C. Pitawala ,&nbsp;Kuruppu M.S. Wimalasiri ,&nbsp;Kahandage F.S.T. Silva","doi":"10.1016/j.bcdf.2025.100486","DOIUrl":"10.1016/j.bcdf.2025.100486","url":null,"abstract":"<div><div>Inulin is a naturally occurring fructose polysaccharide and soluble dietary fiber, found in various plants, commonly used in the food industry for its excellent prebiotic properties and as a fat or sugar substitute. Commercial inulin is largely extracted from Chicory plant, which is grown only in temperate parts of the world. This study chemically and structurally investigated the potential of four Asparagus species <em>A. falcatus</em>, <em>A. racemosus</em>, <em>A. officinalis</em>, and <em>A. gonocladus</em> as alternative inulin sources for the food industry. Inulins were extracted using either hot water extraction or microwave-assisted extraction. Asparagus inulin powders were analyzed for inulin content, total phenolics, total flavonoids, radical scavenging activity and mineral composition. <em>A. falcatus</em>, <em>A. gonocladus</em> and <em>A. racemosus</em> fresh roots contained considerably high inulin contents (23.61 ± 1.04, 17.08 ± 2.58, 12.22 ± 1.51 % FW, respectively). The highest inulin content in their powders (92.31 ± 2 0.89 %) was shown by microwave-assisted extracted <em>A. gonocladus</em> inulin powder. The molecular structure and phase behavior of inulin contained in roots of Asparagus were confirmed by FTIR and XRD respectively, and the thermal behavior was confirmed by DSC data. The results suggest that Asparagus inulin possesses significant potential for use in food applications as an alternative inulin source.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100486"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable biomaterials: Sepia prashadi cuttlebone derived phosphorylated chitosan for oral healthcare","authors":"Padmanaban Anandam ,&nbsp;Yagniyasree Manogaran ,&nbsp;Pasiyappazham Ramasamy","doi":"10.1016/j.bcdf.2025.100485","DOIUrl":"10.1016/j.bcdf.2025.100485","url":null,"abstract":"<div><div>Phosphorylated chitosan indicate potential as a therapy for dental infections, which can be the underlying cause of many dental problems. This study aims to synthesize and characterize phosphorylated chitosan derived from cuttlebone and investigate the impact of different quantities on the growth inhibition of oral pathogenic agents that cause disorders, including <em>Streptococcus mutans</em>, <em>Pseudomonas aeruginosa</em>, <em>E. coli</em>, and <em>Candida tropicalis</em>. The chitin as well as chitosan were extracted from a sample of <em>Sepia prashadi</em> using its cuttlebone. The next stage involved phosphorylating chitosan, which resulted in chitosan derivatives. The antimicrobial effects of phosphorylated chitosan were subsequently evaluated with the well diffusion method. Following that, Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD), were used to analyze and evaluate it. Using the well-diffusion method to test antimicrobial effectiveness, phosphorylated chitosan were found to be an extremely potent antimicrobial substance. This study successfully made phosphorylated chitosan from <em>Sepia prashadi</em> cuttlebone, which were then shown to be effective against bacterial and fungal infections in patients. Quantity strongly influences the antimicrobial activity of phosphorylated chitosan, with higher concentrations demonstrating more effective inhibitory actions. According to these outcomes, chitosan derivatives have promise as a component in medicinal treatments targeting clinical bacteria. The research findings indicate the potential use of <em>Sepia prashadi</em> cuttlebones as a unique biological antimicrobial agent in medicinal applications.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100485"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, structural characterization, and in vitro fermentation properties of okra polysaccharides","authors":"Xin Li,&nbsp;Xiaojun Huang,&nbsp;Shaoping Nie","doi":"10.1016/j.bcdf.2025.100481","DOIUrl":"10.1016/j.bcdf.2025.100481","url":null,"abstract":"<div><div>In this study, two components of okra polysaccharide (OP-12 and OP-14) were isolated and purified. Their chemical structures and <em>in vitro</em> fermentation characteristics were studied to explore the structure-activity relationship in improving intestinal microecology. Results showed that OP-12 and OP-14 had structural differences. Compared with OP-14, OP-12 had higher neutral sugar content, molecular weight (<em>Mw</em>), and degree of branching. The most abundant monosaccharides in OP-12 were rhamnose (Rha) and galactose (Gal), while those in OP-14 were galacturonic acid (GalA). Methylation and nuclear magnetic resonance (NMR) analysis suggested OP-12 and OP-14 were likely pectin polysaccharides containing rhamnogalacturonan I (RG-I) domain and homogalacturonans (HG) domain, respectively. Furthermore, OP-12 and OP-14 with different chemical structures had differences in bacterial density, sugar consumption rate, short-chain fatty acids (SCFAs) production and intestinal microbiota composition during the fermentation process. OP-14 was rapidly utilized by intestinal flora at the early stage, whereas OP-12 was preferentially consumed in the middle-late stages. Correlation analysis revealed that the monosaccharide composition, degree of branching, and <em>Mw</em> of okra polysaccharides were closely related to the regulation effect of the gut microbiota and the types and concentrations of SCFAs.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100481"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the functional benefits of powdered ginger beverage through the incorporation of Ganoderma lucidum","authors":"Anastasia Wheni Indrianingsih,&nbsp;Septi Nur Hayati,&nbsp;Vita Taufika Rosyida,&nbsp;Wuri Apriyana,&nbsp;Cici Darsih,&nbsp;Khoirun Nisa,&nbsp;Ade Erma Suryani,&nbsp;Tri Wiyono,&nbsp;Anjar Windarsih,&nbsp;Sri Handayani","doi":"10.1016/j.bcdf.2025.100482","DOIUrl":"10.1016/j.bcdf.2025.100482","url":null,"abstract":"<div><div>Ginger drinks are one of the traditional drinks that are widely available in Asia, especially Indonesia. <em>Ganoderma lucidum</em> mushroom is also commonly used in traditional medicine but has not been widely used in beverage products. This research aims to produce instant powdered beverages with a combination of ginger and <em>G. lucidum</em> with three ratios, i.e., A = ginger extract: <em>G. lucidum</em> extract = 93.8: 6.2 % (w/w); B = ginger extract: <em>G. lucidum</em> extract 87.4:12.6 % (w/w); C = ginger extract: <em>G. lucidum</em> extract 81.2:18.8 % (w/w) by using sucrose as binder and crystallization agent. Product K was made as a control consisting of 100 % ginger. The results of the antioxidant test with DPPH assay showed that product C had the most significant activity of 59.82 % at 200 μg/ml. FTIR test suggests that the extract contains functional groups of flavonoids, phenolic acids, lipids, and terpenoids. GC-MS analysis showed that several essential oil such as α-curcumene, zingiberene, α-farnesene, β-farnesene, zingerone, and nerolidol along with a fatty amide type compound (9Z)-9-Octadecenamide were identified present in ginger-<em>G. lucidum</em> formula. Using LC-HRMS analysis, the phytochemical components of <em>G. lucidum</em> known to have significant antioxidant activity included (+)-[6]-gingerol, ursolic acid, and 18-β-glycyrrhetinic acid. Meanwhile, the XRD analysis test showed the crystallinity of the ginger-<em>G. lucidum</em> product remained the same as the 100 % ginger product. The results of this study indicate that the addition of <em>G. lucidum</em> to the beverage powder significantly increased antioxidant activity, and <em>G. lucidum</em> has the potential to be used as a source of natural antioxidants in food and pharmaceutical products.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100482"},"PeriodicalIF":0.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microencapsulation of Fridericia chica extract: stability, anthocyanin retention, and antioxidant properties for food and therapeutic applications","authors":"Vitória Ribeiro Garcia de Figueiredo Muniz ,&nbsp;Ailane Souza de Freitas ,&nbsp;Luan da Silva Dias Rabelo ,&nbsp;Luís Felipe Perdigão Rodrigues ,&nbsp;Karina Rodrigues Honorato ,&nbsp;Ana Carolina Rorato ,&nbsp;Ana Lúcia Tasca Gois Ruiz ,&nbsp;Ilza Maria de Oliveira Sousa ,&nbsp;Luciana Igarashi-Mafra ,&nbsp;Mary Ann Foglio ,&nbsp;Alvaro Luiz Mathias ,&nbsp;Marcos R. Mafra","doi":"10.1016/j.bcdf.2025.100483","DOIUrl":"10.1016/j.bcdf.2025.100483","url":null,"abstract":"<div><h3>Relevance</h3><div><em>Fridericia chica</em> (Bonpl.) L.G. Lohmann (syn. <em>Arrabidaea chica</em> Verlot) is a medicinal plant traditionally utilized by Indigenous communities and local populations in the Amazon and tropical South America for its antioxidant, anti-inflammatory, and anticancer activities, attributed to its anthocyanin-rich composition.</div></div><div><h3>Aim of the study</h3><div>To enhance aqueous infusion extraction of processed <em>F. chica</em> leaves to maximize anthocyanin content and stabilize bioactive compounds through microencapsulation via spray drying.</div></div><div><h3>Materials and methods</h3><div>Mineral water acidified with varying concentrations of Tahiti lemon juice was used to extract bioactive compounds. The extract with the highest anthocyanin content was microencapsulated using maltodextrin or Arabic gum as protective matrices. High-Performance Liquid Chromatography (470 nm detector) identified key anthocyanidins (hydroxycarajurone, carajurone, carajurin). Total phenolic compounds (TPC), total flavonoid compounds (TFC), and antioxidant properties (ABTS, DPPH, FRAP) were assessed over 180 days under refrigerated (4 °C) and room temperature (25 °C) storage conditions.</div></div><div><h3>Results</h3><div>The extract microparticles (FEC, control) showed significant degradation in bioactive content (88 % reduction in TPC, 35 % in TFC). Microencapsulation improved stability: Microcapsules with maltodextrin (FEMM) exhibited a 7.15 % loss of total phenolic content (TPC) and a 10.22 % loss of total flavonoid content (TFC), whereas microcapsules with gum Arabic (FEMA) showed minimal loss of TPC (0.74 %) and an 11.66 % reduction of TFC. Refrigeration mitigated degradation, and Arabic gum had better-preserved antioxidant activity than maltodextrin under both conditions.</div></div><div><h3>Conclusions</h3><div>Microencapsulation significantly enhanced the stability of <em>F. chica</em> bioactive compounds, with Arabic gum outperforming maltodextrin. These powders demonstrate high potential as functional food ingredients, offering stability, antioxidant preservation, and suitability for extended storage.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100483"},"PeriodicalIF":0.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative insights into carbohydrate-coated gold nanoparticle-based drug delivery vehicles for QSAR advancement: Calorimetric and mechanistic studies","authors":"Vishakha Choudhary,&nbsp;Aditi Pandey,&nbsp;Bhawna Sharma,&nbsp;Achal Mukhija","doi":"10.1016/j.bcdf.2025.100484","DOIUrl":"10.1016/j.bcdf.2025.100484","url":null,"abstract":"<div><div>Quantitative structure-activity relationship (QSAR) analysis is essential for the development of effective drug delivery vehicles in the era of Artificial Intelligence (AI). To develop this QSAR, carbohydrates with different structural features have been used to coat and make biocompatible gold nanoparticles. These carbohydrate-coated gold nanoparticles have been synthesized by chemical reduction method and characterized by UV–vis, FT-IR, DLS, and SEM techniques. For quantitative study, the interaction of these nanoparticles has been studied with biological interfaces (protein and DNA) and their structural features have correlated with thermodynamic parameters; binding constant, Gibbs free energy, enthalpy, and entropy obtained from calorimetry and fluorescence technique. To elucidate the effect of coating of carbohydrates on gold nanoparticles, interaction studies have also been done with pure carbohydrates and compared with carbohydrate-coated gold nanoparticles. For activity-relationship, these quantitative parameters have been correlated with the therapeutic efficiency of these nanoparticles in terms of drug loading and drug release studies with 5-fluorouracil (5-FU) drugs. The results suggest the importance of the CHO functional group and also the number of monomeric units in interaction studies and therapeutic efficiency. Energetics of interaction correlated with physical characteristics of different carbohydrates develop the structure-activity relationship, which assists in the development of highly effective AI-based future drug delivery vehicles.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100484"},"PeriodicalIF":0.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterococcus hirae OL616073 glucan exopolysaccharide based cumin oil emulsion: Characterization and antibacterial activity","authors":"Swati Tiwari ,&nbsp;Digambar Kavitake ,&nbsp;Palanisamy Bruntha Devi ,&nbsp;Irshad Ahmad Shah ,&nbsp;Prathapkumar Halady Shetty","doi":"10.1016/j.bcdf.2025.100480","DOIUrl":"10.1016/j.bcdf.2025.100480","url":null,"abstract":"<div><div>This study aims to prepare the cumin essential oil-based emulsion stabilized by glucan exopolysaccharide (EPS) from <em>Enterococcus hirae</em> OL616073. The emulsion was prepared by optimizing different ratios of EPS to essential oil via vortexing, ultrasonication, and high shear homogenization. The concentration of EPS (among 0.5, 1, 1.5, and 2 %) and the ratio of EPS to essential oil (among 1:0.5, 1:1, 1:1.5, and 1:2) were initially optimized using vortexing. Ultrasonication and high shear homogenization processes were applied to the vortexed samples and optimized the concentration of EPS and the ratio of EPS to essential oil combinations. The emulsion capacity, emulsion stability, the effect of various processing parameters (temperature and salt), and antimicrobial activity were determined for the emulsions prepared from vortexing, ultrasonication, and high shear homogenization. It was found that emulsion capacity and emulsion stability were better in ultrasonication and high shear homogenization treatment compared to vortexing. When these emulsions were treated with various temperatures and the effect of ionic strengths, the order of emulsion stability was recorded as ultrasonication &gt; high shear homogenization &gt; vortexing. Further, there was significant antimicrobial activity against four tested pathogens, namely <em>Listeria monocytogenes</em> (MTCC 1143), <em>S. aureus</em> (MTCC 1144), <em>E. coli</em> (MTCC 433), and <em>Shewanella putrefaciens</em> (MTCC 8104), but no antimicrobial activity against <em>Salmonella enterica</em> (950) was observed. Parameters such as type of emulsion, turbidity, pH, Fourier transform infrared (FT-IR) analysis, and microscopic analyses were also performed for emulsions. This EPS-based emulsion system can be further explored for functional applications in the field of food and health.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100480"},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}