Current Plant Biology最新文献

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Cryopreservation of Arum palaestinum plant callus as a strategy for mitigating extinction risks 低温保存旱金莲植物胼胝体作为降低灭绝风险的一种策略
IF 5.4
Current Plant Biology Pub Date : 2024-10-18 DOI: 10.1016/j.cpb.2024.100402
Sobhia Saifan , Rida A. Shibli , Tamara S. Al Qudah , Reham W. Tahtamouni , Tamadour Al –Qudah
{"title":"Cryopreservation of Arum palaestinum plant callus as a strategy for mitigating extinction risks","authors":"Sobhia Saifan ,&nbsp;Rida A. Shibli ,&nbsp;Tamara S. Al Qudah ,&nbsp;Reham W. Tahtamouni ,&nbsp;Tamadour Al –Qudah","doi":"10.1016/j.cpb.2024.100402","DOIUrl":"10.1016/j.cpb.2024.100402","url":null,"abstract":"<div><div><em>Arum palaestinum</em> is a wild perennial plant commonly known as \"Al-Loof\" in Jordan. Due to overharvesting, climate change, and increasing demand, its natural populations are threatened with extinction. Cryopreservation, an effective method for conserving plant material at ultra-low temperatures, is explored for <em>A. palaestinum</em> calli. We investigated the applicability of encapsulation-vitrification (using different plant vitrification solutions (PVS) and incubation times), encapsulation-dehydration (using sucrose or sorbitol at different concentrations and dehydration times), and the v-cryoplate (using different pre-culture times and temperatures) techniques. In the encapsulation-vitrification experiment, a notable 82.4 % regrowth rate was achieved by desiccating calli in plant vitrification solution 2 (PVS2) for 10 minutes at 25 °C. The encapsulation-dehydration technique resulted in an 82.6 % regrowth rate by incubating calli for one day in low sucrose levels (0.1 M sucrose) following one hour of air dehydration, where the moisture content of the beads was 30 %. The moisture content of the beads decreased from 81 % before chemical and air dehydration to 71 % after 0 hours of air dehydration combined with chemical dehydration using 0.1 M sucrose or sorbitol. It further dropped to 30–34 % after one day of chemical dehydration with 0.1 M sucrose and 1 hour of air dehydration. The v-cryoplate technique successfully conserved calli, showing impressive survival and regrowth percentages (96.8 %) when the callus was pre-cultured with 0.3 M sucrose for three days at 5 °C. Temperature during pre-culture significantly influenced regrowth percentages in the v-cryoplate technique. The study establishes promising cryopreservation protocols for <em>A. palaestinum</em> calli, offering a means to conserve germplasm and contribute to environmental and biodiversity protection by reintroducing endangered plants to their native habitats.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100402"},"PeriodicalIF":5.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Promotion of gentiopicroside production and transcriptional responses of biosynthetic genes in adventitious root cultures of Gentiana scabra Bunge by elicitation with methyl jasmonate 用茉莉酸甲酯诱导促进龙胆草不定根培养物中龙胆内酯的产生和生物合成基因的转录反应
IF 5.4
Current Plant Biology Pub Date : 2024-10-17 DOI: 10.1016/j.cpb.2024.100397
Jingwen Hao , Jian Zang , Dan Liu , Ning Hao , Rujun Zhou
{"title":"Promotion of gentiopicroside production and transcriptional responses of biosynthetic genes in adventitious root cultures of Gentiana scabra Bunge by elicitation with methyl jasmonate","authors":"Jingwen Hao ,&nbsp;Jian Zang ,&nbsp;Dan Liu ,&nbsp;Ning Hao ,&nbsp;Rujun Zhou","doi":"10.1016/j.cpb.2024.100397","DOIUrl":"10.1016/j.cpb.2024.100397","url":null,"abstract":"<div><div>Abiotic elicitors play a crucial role in regulating various aspects of plant growth, development, and specialized metabolism. This study aimed to further increase the gentiopicroside content by screening elicitor types, optimizing elicitation conditions, and estimating transcriptional responses of biosynthetic genes in the adventitious roots of <em>Gentiana scabra</em>. The results showed that methyl jasmonate (MeJA) was the most effective inducer for biomass accumulation in the adventitious roots of <em>G. scabra</em> among tested elicitors, with fresh weight (FW) and dry weight (DW) of 13.26 ± 0.57 g flask<sup>−1</sup> and 1.31 ± 0.25 g flask<sup>−1</sup>, respectively. The effects of the induction time and concentration of MeJA on the biomass and gentiopicroside content in the adventitious roots of <em>G. scabra</em> were investigated. The maximum FW (15.73 ± 0.41 g flask<sup>−1</sup>) and DW (1.51 ± 0.19 g flask<sup>−1</sup>) were obtained when the roots were cultured for 6 days in MS liquid medium containing 3.0 mg L<sup>−1</sup> 1-naphthlcetic acid (NAA) and 1.0 mg L<sup>−1</sup> kinetin (KT) at MeJA concentration of 100 μM L<sup>−1</sup>. Also, the gentiopicroside content significantly increased to 62.62 ± 0.27 mg g<sup>−1</sup> DW, and was 2.49 times higher than that for the nontreated control. The expression levels of 12 candidate gentiopicroside biosynthesis–related genes involved in the mevalonic acid (MVA), methyl erythritol phosphate (MEP), and secoiridoid pathways were estimated in the adventitious roots of <em>G. scabra</em> to further understand the transcriptional response to MeJA elicitation. Among these, 10 genes (<em>ACCT1</em>, <em>HMGR1</em>, <em>MCK1</em>, <em>MVD1</em>, <em>GPPS4</em>, <em>G10H</em>, <em>IS3</em>, <em>DL7H1</em>, <em>DXS5</em>, and <em>ISPH5</em>) were upregulated whereas <em>DXR1</em> and <em>IDI1</em> genes were downregulated in the adventitious roots of <em>G. scabra</em> compared with nontreated control, with significant differences having threshold <em>P</em> value ≤0.05. The transcriptional analyses revealed that 12 candidate genes were the key regulated genes in the gentiopicroside biosynthetic pathway. Overall, the findings provided a promising, feasible, and stable approach to utilizing MeJA elicitation to increase the production of valuable gentiopicroside. Additionally, they provided a foundation for future gentiopicroside biosynthesis through metabolic engineering strategies in the adventitious roots of <em>G. scabra</em>.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100397"},"PeriodicalIF":5.4,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temporal changes in the proanthocyanidins to anthocyanins ratio during dormancy associate with bloom time variations in peach 桃子休眠期原花青素与花青素比率的时间变化与开花时间的变化有关
IF 5.4
Current Plant Biology Pub Date : 2024-10-15 DOI: 10.1016/j.cpb.2024.100393
Protiva Rani Das , Md Tabibul Islam , Jianyang Liu , Zongrang Liu , Chris Dardick , Sherif M. Sherif
{"title":"Temporal changes in the proanthocyanidins to anthocyanins ratio during dormancy associate with bloom time variations in peach","authors":"Protiva Rani Das ,&nbsp;Md Tabibul Islam ,&nbsp;Jianyang Liu ,&nbsp;Zongrang Liu ,&nbsp;Chris Dardick ,&nbsp;Sherif M. Sherif","doi":"10.1016/j.cpb.2024.100393","DOIUrl":"10.1016/j.cpb.2024.100393","url":null,"abstract":"<div><div>Our previous research demonstrated that fall applications of ethephon, an ethylene-releasing plant growth regulator, delay bloom in peach, accompanied by changes in endogenous hormones, ROS, sugar metabolism, and transcriptomic profiles during bud dormancy phases (endodormancy and ecodormancy). In this study, floral bud tissues were collected from ethephon-treated and untreated trees at three time points (200, 600, and 1000 chilling hours, CH) during endodormancy and two points (1000 and 3000 growing degree hours, GDH) during ecodormancy. Using ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF/MS), we aimed to unravel the untargeted metabolic changes explaining ethephon-mediated bloom delay. Metabolite set-enrichment analysis (MSEA) revealed significant chemical group variations between dormancy phases, with a threefold increase in flavonoids during endodormancy and a doubling of organic and amino acids during ecodormancy. Further analysis of genes associated with the biosynthesis and transcriptional regulation of the flavonoid pathway showed that ethephon treatment upregulated genes associated with proanthocyanidin (PA) biosynthesis and downregulated genes related to anthocyanins (ACNs). We quantified PA and ACN contents in 12 peach cultivars with contrasting bloom times and chilling requirements (e.g. 727–1308 CH). Late-bloom cultivars had higher PA levels during endodormancy, while early-bloom cultivars had higher ACN levels during ecodormancy. Staining buds with 4-dimethylaminocinnamaldehyde (DMAC) dye revealed a decline in the PA/ACN ratio at later ecodormancy stages, correlating with bloom time. Integrated analysis of metabolite content and gene expression in late-bloom 'KV021779' and early-bloom 'John Boy' cultivars validated that late-blooming cultivars have higher PA levels during endodormancy, extending dormancy-release periods and resulting in later blooms.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100393"},"PeriodicalIF":5.4,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield 转录因子 TaNF-YB4 在小麦中的过表达可提高植物活力和产量
IF 5.4
Current Plant Biology Pub Date : 2024-10-10 DOI: 10.1016/j.cpb.2024.100394
Arooj Azhar, Sidra Ijaz, Ayesha Jabeen, Attiya Kamal, Aftab Bashir, Kauser Abdulla Malik
{"title":"The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield","authors":"Arooj Azhar,&nbsp;Sidra Ijaz,&nbsp;Ayesha Jabeen,&nbsp;Attiya Kamal,&nbsp;Aftab Bashir,&nbsp;Kauser Abdulla Malik","doi":"10.1016/j.cpb.2024.100394","DOIUrl":"10.1016/j.cpb.2024.100394","url":null,"abstract":"<div><div>Addressing food security is a priority in developing countries. This study aimed to improve wheat yield by overexpressing the <em>TaNF-YB4</em> transcription factor, which is involved in carbon assimilation and stress tolerance. An expression cassette for <em>TaNF-YB4</em> was developed in a modified wheat transformation vector (pSB219) and examined through transient expression in <em>Nicotiana tabacum</em>, followed by <em>Agrobacterium</em>-mediated transformation of wheat variety FSD-2008. T<sub>0</sub> transgenic plants were propagated to obtain T<sub>3</sub> generation PCR-positive plants. Transgene expression was assessed in PCR-verified T<sub>2</sub> plants using RT-PCR and qRT-PCR at six weeks post-germination. qRT-PCR analysis using the ΔΔCT method indicated higher <em>TaNF-YB4</em> expression in transgenic lines than in the wild-type control plants. Improved agronomic and phenotypic traits were observed with a 6–36 % increase in 1000-grain weight in the selected transgenic lines. Root architecture assessments demonstrated enhanced root length, surface area, and projected area in transgenic lines compared with wild-type plants. Additionally, notable variances in total chlorophyll, protein, and sugar content levels were observed between the transgenic lines and control plants, demonstrating statistical significance with a p-value ≤ 0.05. This study indicates that low-level constitutive expression of <em>TaNF-YB4</em> can enhance wheat yield, presenting a viable strategy for improving wheat productivity.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100394"},"PeriodicalIF":5.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green-synthesized carbon dots from ginger: Multifunctional agents against oral pathogens with biocompatibility in human gingival fibroblast cells 生姜绿色合成碳点:在人牙龈成纤维细胞中具有生物相容性的抗口腔病原体多功能制剂
IF 5.4
Current Plant Biology Pub Date : 2024-10-09 DOI: 10.1016/j.cpb.2024.100392
Shaeesta Khaleelahmed Bhavikatti , Siti Lailatul Akmar Zainuddin , Rosmaliza Binti Ramli , Sameer J. Nadaf , Padma B. Dandge , Masidd Khalate , Mohmed Isaqali Karobari
{"title":"Green-synthesized carbon dots from ginger: Multifunctional agents against oral pathogens with biocompatibility in human gingival fibroblast cells","authors":"Shaeesta Khaleelahmed Bhavikatti ,&nbsp;Siti Lailatul Akmar Zainuddin ,&nbsp;Rosmaliza Binti Ramli ,&nbsp;Sameer J. Nadaf ,&nbsp;Padma B. Dandge ,&nbsp;Masidd Khalate ,&nbsp;Mohmed Isaqali Karobari","doi":"10.1016/j.cpb.2024.100392","DOIUrl":"10.1016/j.cpb.2024.100392","url":null,"abstract":"<div><div>Persistent antibiotic use in treating oral infections often leads to drug resistance in pathogenic bacteria, notably impacting conditions like periodontitis. Addressing this challenge, the study pioneers the use of carbon dots (CDs) synthesized from ginger rhizomes (<em>Zingiber officinale</em>) as a novel biocompatible material. CDs were synthesized via the hydrothermal method, emphasizing a green approach, and comprehensively characterized for their optical properties and structural uniformity. The synthesized CDs showed a zeta potential of −24.9 mV, confirming the formation of stable and well-dispersed particles. Dynamic Light Scattering (DLS) confirmed an average particle size of 2.9 nm, thus validating the formation of CDs. Biomedical assessments demonstrated that the synthesized CDs were non-cytotoxic to human gingival fibroblast cell lines, with effective free radical scavenging activity and high total antioxidant capacity, as indicated by their IC50 values. CDs also exhibited moderate inhibition of protein denaturation compared to the standard. Moreover, they showed significant inhibitory effects against bacterial strains (<em>Pseudomonas aeruginosa</em>, <em>Lactobacillus acidophilus</em>, <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>) and fungal strains (<em>Aspergillus niger</em>, <em>Candida albicans</em>) at minimal concentrations. Notably, CDs inhibited the growth of periodontal pathogens including <em>Aggregatibacter actinomycetemcomitans</em>, <em>Tannerella forsythia</em>, <em>Porphyromonas gingivalis</em>, and <em>Prevotella intermedia</em>. These findings underscore the potential of CDs as multifunctional agents possessing anti-inflammatory, antifungal, antioxidant, and antibacterial properties. Remarkably, they offer a promising alternative to conventional antibiotics, potentially revolutionizing oral healthcare. Their proven biocompatibility and potent bioactivity underscore their innovative potential in biomedical research. Future studies should further assess their efficacy <em>in vivo</em> to fully harness their clinical potential.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100392"},"PeriodicalIF":5.4,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-depth genome-wide characterization of MaNAC25 and MaNAC28 cold-responsive transcription factor binding sites in banana via DAP-Seq 通过 DAP-Seq 深入分析香蕉中 MaNAC25 和 MaNAC28 冷响应转录因子结合位点的全基因组特征
IF 5.4
Current Plant Biology Pub Date : 2024-10-06 DOI: 10.1016/j.cpb.2024.100389
Chunbo Song
{"title":"In-depth genome-wide characterization of MaNAC25 and MaNAC28 cold-responsive transcription factor binding sites in banana via DAP-Seq","authors":"Chunbo Song","doi":"10.1016/j.cpb.2024.100389","DOIUrl":"10.1016/j.cpb.2024.100389","url":null,"abstract":"<div><div>Mapping transcription factor proteins' binding sites across the entire genome in banana is crucial for unveiling their transcriptional regulatory mechanisms and enhancing our understanding of their regulatory networks. Our study showed that DAP-Seq experiments identified MaNAC25 and MaNAC28 numerous binding peaks, mainly in the promoter regions, with strong signals near the transcription start site (TSS). Significantly, the discovery of new binding motifs for MaNAC28 excluding NAC core binding element CGTA/G indicates their potential as novel DNA binding motifs for NAC transcription factors in cold stress response. Moreover, MaNAC25 was found to chiefly influence biological processes and molecular functions, whereas MaNAC28 was more focused on molecular functions. Both MaNAC25 and MaNAC28 extended their regulatory networks by interacting with other transcription factors during cold stress. Therefore, DAP-Seq technology furnishes essential insights and a robust foundation for researching transcriptional regulatory mechanisms among diverse transcription factors and broadening their regulatory networks.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100389"},"PeriodicalIF":5.4,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic dissection of flour whiteness through genome-wide association analysis in common wheat (Triticum aestivum L.) 通过全基因组关联分析剖析普通小麦(Triticum aestivum L.)
IF 5.4
Current Plant Biology Pub Date : 2024-10-05 DOI: 10.1016/j.cpb.2024.100391
Jinna Hou , Hui Deng , Fuxin Xu , Shenghui Geng , Congcong Liu , Wenxu Li , Xia Shi , Zhengqing Wu , Zhensheng Lei , Zhengfu Zhou
{"title":"Genetic dissection of flour whiteness through genome-wide association analysis in common wheat (Triticum aestivum L.)","authors":"Jinna Hou ,&nbsp;Hui Deng ,&nbsp;Fuxin Xu ,&nbsp;Shenghui Geng ,&nbsp;Congcong Liu ,&nbsp;Wenxu Li ,&nbsp;Xia Shi ,&nbsp;Zhengqing Wu ,&nbsp;Zhensheng Lei ,&nbsp;Zhengfu Zhou","doi":"10.1016/j.cpb.2024.100391","DOIUrl":"10.1016/j.cpb.2024.100391","url":null,"abstract":"<div><div>The color of flour products has an important influence on consumer acceptance. Flour color is largely determined and measured by the index of flour whiteness (FW) in China. In this study, an association population comprising 207 wheat (<em>Triticum aestivum</em>) accessions originating from seven countries was used for dissection of FW-related genetic loci through genome-wide association analysis. Six quantitative trait loci (QTLs) significantly associated with FW were identified, accounting for 7.87–16.53 % of the total phenotypic variation. Four KASP markers were developed from single-nucleotide polymorphisms associated with the QTLs <em>QFW.HAAS</em>-<em>1AS</em>, <em>QFW.HAAS-1BL</em>, <em>QFW.HAAS-5AL</em>, and <em>QFW.HAAS-7AL</em>. The phytoene synthase-encoding gene <em>TraesCS7A03G1357000</em> (<em>TaPsyA1</em>) was identified as a candidate gene for <em>QFW.HAAS-7AL</em>. Two allelic variants of <em>TaPsyA1</em> (designated <em>PsyA1-a</em> and <em>PsyA1-b</em>) were differentiated on the basis of a 37 bp insertion/deletion polymorphism in the second intron. <em>PsyA1-b</em> included the 37 bp insertion, which led to a translational frameshift in the gene and was associated with higher FW. The <em>PsyA1-a</em> allele lacked the 37 bp insertion and was classified into two haplotypes according to the number of repeated ‘TC’ units in a simple sequence repeat in the promoter region. Of the two <em>PsyA1-a</em> haplotypes, the Type 1 haplotype conferred higher FW, flour brightness, and flour redness, and lower yellow pigment content and flour yellowness. The KASP markers and <em>PsyA1</em> polymorphic markers developed in the present study are suitable for use in molecular marker-assisted selection for improvement of wheat FW.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100391"},"PeriodicalIF":5.4,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Serratia sp. strain C2 confers tomato tolerance to high salt, virus infection and both stresses in combination Serratia sp. 菌株 C2 可使番茄耐受高盐、病毒感染以及两种胁迫的共同作用
IF 5.4
Current Plant Biology Pub Date : 2024-10-04 DOI: 10.1016/j.cpb.2024.100390
Naima Sayahi , Giorgia Sportelli , Anna Vittoria Carluccio , Chantal Ebel , Tahar Mechichi , Fabrizio Cillo , Moez Hanin , Livia Stavolone
{"title":"The Serratia sp. strain C2 confers tomato tolerance to high salt, virus infection and both stresses in combination","authors":"Naima Sayahi ,&nbsp;Giorgia Sportelli ,&nbsp;Anna Vittoria Carluccio ,&nbsp;Chantal Ebel ,&nbsp;Tahar Mechichi ,&nbsp;Fabrizio Cillo ,&nbsp;Moez Hanin ,&nbsp;Livia Stavolone","doi":"10.1016/j.cpb.2024.100390","DOIUrl":"10.1016/j.cpb.2024.100390","url":null,"abstract":"<div><div>Besides increasing plant growth, several Plant Growth Promoting Rhizobacteria (PGPR), can enhance tolerance to biotic and/or abiotic stresses of numerous plant species. While cultivated plants are frequently subject to combined stresses in the field, there is limited knowledge of the effect of PGPR on plants undergoing simultaneous stress conditions. Therefore, we tested the beneficial properties of the halotolerant PGPR <em>Serratia</em> sp. strain C2, previously shown to enhance salt stress tolerance in barley, on tomato plants exposed to salinity, to Potato Virus Y (PVY) infection, and both stresses simultaneously. In our experimental conditions, C2 inoculation improved tomato tolerance to salt stress and positively correlated with a 46–68 % decrease in the level of PVY RNA compared to non-inoculated tomato plants. Morphometric, physiological and biochemical analyses (e.g., chlorophyll, sugar and proline accumulation, oxidative stress status and NDVI) indicated that C2 treatments had beneficial effects on tomato growth under simple and combined stress conditions. This is the first report of a PGPR enhancing tolerance not only to individually induced salinity and PVY infection, but also to both stresses in combination. Moreover, the expression analysis of selected genes involved in stress responses and RNA silencing-mediated antiviral immunity suggests that C2 can interfere with distinct defence response pathways to enhance stress tolerance in tomato. These pioneering results support the perspective of using PGPR as multi-spectrum and multi-host biostimulants for improving plant growth and protection from biotic, abiotic, and combined stresses to promote sustainable crop production in the face of environmental changes.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100390"},"PeriodicalIF":5.4,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing sweet cherry resilience to spring frost and rain-induced cracking with pre-harvest melatonin treatments 通过采收前褪黑激素处理增强甜樱桃对春季霜冻和雨水引起的裂果的抗逆性
IF 5.4
Current Plant Biology Pub Date : 2024-09-26 DOI: 10.1016/j.cpb.2024.100388
M.C. Ruiz-Aracil , J.M. Valverde , A. Beltrà , J.M. Lorente-Mento , A. Carrión-Antolí , D. Valero , F. Guillén
{"title":"Enhancing sweet cherry resilience to spring frost and rain-induced cracking with pre-harvest melatonin treatments","authors":"M.C. Ruiz-Aracil ,&nbsp;J.M. Valverde ,&nbsp;A. Beltrà ,&nbsp;J.M. Lorente-Mento ,&nbsp;A. Carrión-Antolí ,&nbsp;D. Valero ,&nbsp;F. Guillén","doi":"10.1016/j.cpb.2024.100388","DOIUrl":"10.1016/j.cpb.2024.100388","url":null,"abstract":"<div><div>Sweet cherry producers annually confront climatic challenges such as spring frost and fruit cracking. This vulnerability arises primarily from spring frost during bloom or cracking at critical maturity stages during persistent rainfall. With changing climate patterns, innovative strategies are essential to mitigate these adversities. Foliar applications of melatonin (MT) at 0, 0.01, 0.05 and 0.1 mM were tested on the ‘Prime Giant’ and ‘Sweetheart’ cultivars over four different production cycles (2020–2023) to evaluate the effect on frost resilience on flower buds and fruit cracking reduction. MT-treated flower buds showed reduced malondialdehyde content and increased fruit set in most seasons, reducing their vulnerability to extreme weather events. In addition, MT consistently decreased sweet cherry cracking incidence across all studied seasons, indicating a strong effect between the fruit ripening stage and susceptibility to fruit cracking, which was cultivar dependent. Quality parameters at harvest, including fruit firmness, and colour at harvest, were either delayed or unaffected in MT-treated fruits compared with controls. However, other ripening parameters were stimulated by pre-harvest MT applications in several growing cycles, such as total soluble solids, which slightly reduced total acidity in MT-treated fruits. In summary, pre-harvest MT treatments can be a promising strategy for climate change adaptation and stress mitigation, potentially increasing sweet cherry production under extreme weather conditions.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100388"},"PeriodicalIF":5.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LED light treatments enhance neuroprotective properties and differentially impact phenolic compounds and triterpenoid content in Gotu Kola (Centella asiatica (L.) Urb.) LED 光处理可增强五加科拉(积雪草)的神经保护特性,并对其酚类化合物和三萜类化合物的含量产生不同影响
IF 5.4
Current Plant Biology Pub Date : 2024-09-11 DOI: 10.1016/j.cpb.2024.100386
Melissa Soun-udom , Maria Rita Garcia , Andreia P. Oliveira , Paula B. Andrade , Srunya Vajrodaya , Sutsawat Duangsrisai , Nelson G.M. Gomes
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