Current Plant Biology最新文献

{"title":"Genome-wide identification of Uncaria rhynchophylla bHLH transcription factors and in-vitro validation of UrbHLH1 through interaction with terpenoid indole alkaloid synthesis pathway members","authors":"Yingying Shao ,&nbsp;Detian Mu ,&nbsp;Limei Pan ,&nbsp;Zhiguo Lu ,&nbsp;Yu Zhou ,&nbsp;Huan Zhao ,&nbsp;Iain W. Wilson ,&nbsp;Ying Lu ,&nbsp;Lina Zhu ,&nbsp;Yao Zhang ,&nbsp;Xinghui Liu ,&nbsp;Ya Qin ,&nbsp;Deyou Qiu ,&nbsp;Qi Tang","doi":"10.1016/j.cpb.2024.100330","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100330","url":null,"abstract":"<div><p><em>Uncaria rhynchophylla</em> (Gouteng), as an evergreen woody vine belong to <em>Rubiaceae</em> family, is a traditional medicinal herb in China. Its terpenoid indole alkaloids (TIAs), which have good antidepressant and combined therapeutic effects on Alzheimer's disease, have attracted widespread attention. However, the content of TIAs is relatively low in <em>U.rhynchophylla</em>, which is unable to meet the growing market demand. The basic helix loop-helix (bHLH) transcription factor family exists in all three eukaryotic kingdoms and can participate in regulating secondary metabolite pathways. So far, there has been no comprehensive analysis of the <em>bHLH</em> gene in <em>U. rhynchophylla</em>, and their role in TIAs is almost unknown. In this study, a total of 171 <em>UrbHLH</em> genes (<em>UrbHLHs</em>) were unevenly distributed on 22 chromosomes and divided into 23 subfamilies. In addition, the physicochemical properties of UrbHLHs were analyzed. Most <em>UrbHLHs</em> in each subgroup had similar gene structures and conserved motifs. Intraspecific collinearity analysis showed that UrbHLH1 may be related to the biosynthesis of TIAs. Subcellular localization experiments revealed that UrbHLH1 is located in the nucleus; Dual luciferase reporter gene analysis (Dual-LUC) showed that UrbHLH1 could activate the expression of <em>UrG10H</em> and <em>Ur10HGO</em> in the TIAs synthesis pathway of <em>U. rhynchophylla</em>. Finally, using yeast one hybrid (Y1H) it was found that the promoter regions of these two genes both have E-box binding elements, which can be bound by UrbHLH1 and produced strong interactions. Therefore, UrbHLH1 may participate in the synthesis of TIAs pathway. In conclusion, this study provides foundation data on the role of UrbHLH transcription factors in regulating TIAs of <em>U. rhynchophylla</em>.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"38 ","pages":"Article 100330"},"PeriodicalIF":5.4,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000124/pdfft?md5=6355161671edaab66fc9b1a48c4fe56a&pid=1-s2.0-S2214662824000124-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139743959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron biofortification in wheat: Past, present, and future","authors":"Mohammad Jafar Tanin ,&nbsp;Dinesh Kumar Saini ,&nbsp;Pankaj Kumar ,&nbsp;Santosh Gudi ,&nbsp;Himanshu Sharma ,&nbsp;Jatinder Paul Kaur ,&nbsp;Omer Abassy ,&nbsp;Ferdaws Bromand ,&nbsp;Achla Sharma","doi":"10.1016/j.cpb.2024.100328","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100328","url":null,"abstract":"<div><p>Iron (Fe) deficiency is a pressing global health concern, particularly affecting vulnerable groups like women and children in resource-limited areas. Addressing this challenge requires innovative solutions, and biofortified crops, like Fe-enriched wheat, can offer a sustainable solution to improve nutrition in cereal-based diets. While conventional breeding methods have yielded competitive Fe-biofortified wheat varieties across various nations, the imminent challenges in securing food and nutritional security for the future necessitate a delicate balance: maintaining genetic progress in grain yield while concurrently elevating grain Fe content. Despite substantial strides in elucidating the intricacies of Fe homeostasis, there remains a substantial knowledge gap, especially in the context of wheat and similar crop species. It is paramount to gain a comprehensive understanding of the hurdles impeding Fe enrichment in plant tissues and delve into the diverse mechanisms governing Fe uptake, translocation, transport, and storage within wheat. To surmount these challenges, researchers have explored a multitude of strategies, including mutagenesis, QTL mapping, meta-QTL analysis, GWAS, transgenesis, and genome editing. Furthermore, harnessing the potential of microorganisms, particularly engineered endophytes coupled with plant genes associated with Fe accumulation, emerges as a promising and pragmatic tool for augmenting Fe biofortification in wheat. This comprehensive review underscores the significant advancements made in unravelling the genetic and genomic aspects of Fe accumulation in wheat, while also delineating the future research directions in this field. By synergistically deploying these multifaceted approaches, scientists hold the potential to develop wheat varieties characterized by enhanced grain Fe content, improved bioavailability, and reduced anti-nutritional factors. Such innovations can play a pivotal role in advancing nutrition and health outcomes for populations reliant on wheat-based diets, particularly in resource-scarce regions.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"38 ","pages":"Article 100328"},"PeriodicalIF":5.4,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000100/pdfft?md5=5cc4f602ee88ee855558474bbb78b975&pid=1-s2.0-S2214662824000100-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139743859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining transcriptome and untargeted metabolome analysis to reveal the potential mechanism of 24-epibrassinolide alleviating low light stress in tomato","authors":"Jiawei Liu ,&nbsp;Yongxiang Huang ,&nbsp;Sijia Liu,&nbsp;Long Cheng,&nbsp;Duo Xu,&nbsp;Yu Ling,&nbsp;Rongchao Yang,&nbsp;Yueqin Zhang","doi":"10.1016/j.cpb.2024.100327","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100327","url":null,"abstract":"<div><p>Low light stress seriously affects the growth and yield of crops and the phytohormone brassinosteroid (BRs) plays a vital role in regulating plant adaptation to low light conditions. However, the molecular mechanism underlying this process remains largely unknown. In this study, we showed that exogenous BR effectively alleviated damages to photosynthesis and antioxidant systems, improved the plant biomass under low light stress mimicking treatments in tomato (<em>Lycopersicon esculentum</em> Mill.). Comparative transcriptome profiling analysis revealed that genes related with photosynthesis and Calvin cycle pathways were enriched among the differentially expressed genes (DEG) co-regulated by low light stress and BR. The combination of transcriptome and metabolome analysis showed that BR could mitigate the down-regulation of photosynthesis and Calvin cycle caused by low light stress, and partially restore the up-regulation of Glycolysis / Gluconeogenesis and tricarboxylic acid (TCA) cycle through transcriptional and metabolic reprogramming to alleviate the effects of low light stress. Moreover, we further identified the crucial transcription factors, <em>SIEPR1</em> and <em>SIERF059</em>, and their potential target genes involved in the regulation of low light stress alleviation mediated by BR signaling. Our results shed new light on the molecular mechanisms underlying the alleviation of low light stress by BR.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"38 ","pages":"Article 100327"},"PeriodicalIF":5.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000094/pdfft?md5=ed8622251546e897ffd34b8f5136acf3&pid=1-s2.0-S2214662824000094-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genome-wide association study identifies novel QTL for wheat yield stability under drought stress","authors":"Xiaoqiang Liu ,&nbsp;Zhaolin Yang ,&nbsp;Wenjia Hu ,&nbsp;Sitong Liu ,&nbsp;Runze Sun ,&nbsp;Songsong Jin ,&nbsp;Khandmaa Nergui ,&nbsp;Guangyao Zhao ,&nbsp;Lifeng Gao ,&nbsp;Yongxiu Liu ,&nbsp;Xin Deng","doi":"10.1016/j.cpb.2024.100326","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100326","url":null,"abstract":"<div><p>Global climate changes have led to severe and frequent drought stress in many areas, seriously threatening the stability of yield in wheat. Exploring the quantitative trait loci for yield stability under drought condition is needed for wheat molecular breeding. In this study, we collected a panel of 432 diverse wheat accessions from different regions around the world and evaluated the drought stress susceptibility index of wheat yield-related phenotypes under drought stress and genotyped the panel with the wheat660K SNP array. Genome-wide association analysis has identified 40 yield stability-related loci, which distribute on various chromosomes. Four loci on Chromosome 1 A, 2B, 3 A and 7B are associate with more than two phenotypic indicators, explaining 1.59% − 8.07% of the phenotypic variation. Among them, Qgns.cas-3A.2 is a novel QTL for wheat yield stability under drought stress. Venn diagram analysis on the drought-responsive expression patterns of Qtgw.cas-3A genes in drought-tolerant and sensitive cultivars, and linkage disequilibrium analysis of the 57Kb region flanking SNP marker AX-108784842 on chromosomes 3 A which displays the highest confidence identified the same candidate gene, which encodes a glycosyl hydrolases family 17 protein. Haplotype analysis indicated that GG allele of this gene is the favorable allele for wheat yield stability under drought. Taken together, these results provide new insights on understanding the genetic basis of wheat yield stability under drought stress and new tools for developing molecular markers to engineer drought-tolerant wheat cultivars.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100326"},"PeriodicalIF":5.4,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000082/pdfft?md5=c9b2ee561076bb10ff5fde68be277c1e&pid=1-s2.0-S2214662824000082-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139699293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of cotton CrRLK1L family genes and potential function of GhCrRLK1L104 in cell elongation","authors":"Dongyun Zuo ,&nbsp;Lijin Chen ,&nbsp;Javaria Ashraf ,&nbsp;Hailiang Cheng ,&nbsp;Youping Zhang ,&nbsp;Qiaolian Wang ,&nbsp;Limin Lv ,&nbsp;Shang Liu ,&nbsp;Guoli Song","doi":"10.1016/j.cpb.2024.100325","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100325","url":null,"abstract":"<div><p><em>Catharanthus roseus</em> receptor-like kinase 1-like (<em>CrRLK1L</em>) proteins play important roles in cell growth, plant morphogenesis, reproduction, hormone signaling, plant immunity and stress responses in <em>Arabidopsis</em>. However, not much information is available about their functions in cotton. We identified a total of 125, 73 and 71 full-length putative <em>CrRLK1L</em> genes in <em>G. hirsutum</em>, <em>G. arboreum</em> and <em>G. raimondii,</em> which are much greater than that of the other plants. The phylogenetic and gene structure analysis divided the cotton <em>CrRLK1L</em> genes into six major groups, among which only group I and II contained <em>AtCrRLK1L</em>s of <em>Arabidopsis</em>. Genome collinearity analysis revealed large scale reciprocal translocations on chromosome 2 among <em>Gossypium</em> A genomes species, which led to uneven distribution of <em>CrRLK1L</em> genes on this chromosome. In addition, transcriptome data combined with qRT-PCR analysis showed some <em>GhCrRLK1L</em>s were preferentially expressed in fibers during the specific stages of ovules or fibers development. Notably, <em>GhCrRLK1L104</em> was highly expressed in fibers at 30 days post anthesis, and the GhCrRLK1L104::GFP fusion protein was located on the plasma membrane. Furthermore, overexpression of the <em>GhCrRLK1L104</em> gene in <em>Arabidopsis</em> increased the trichomes length of the rosette leaves, indicated its vital roles in cell elongation. These results provided a strong foundation to further explore the molecular mechanism of <em>CrRLK1L</em> genes in upland cotton in cell elongation, that can be used in future cotton breeding program.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100325"},"PeriodicalIF":5.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000070/pdfft?md5=c0bdffa1523ca5e3e89bfa3efc148d5f&pid=1-s2.0-S2214662824000070-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of stress response in Chinese cabbage through eugenol and clove essential oil","authors":"Li-Ching Hsieh ,&nbsp;Zhi Thong Soh ,&nbsp;Wei-An Tsai ,&nbsp;Ting-Fang Chen ,&nbsp;Hsin-Hung Lin ,&nbsp;Jeng-Shane Lin","doi":"10.1016/j.cpb.2024.100324","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100324","url":null,"abstract":"<div><p>Chinese cabbage (<em>Brassica rapa</em> subsp. <em>pekinesis</em>) is a vital leafy vegetable crop that thrives within the temperature range of 12 °C to 22 °C. However, high temperatures can adversely impact its growth, development, and yield. To address this issue, we investigated the potential of eugenol and clove essential oil in enhancing stress tolerance in Chinese cabbage. Transcriptome profiling of Chinese cabbage exposed to eugenol, clove essential oil, and heat stress revealed significantly differentially expressed genes (DEGs). Gene set enrichment analysis indicated that treatment with eugenol and clove essential oil significantly influenced defense responses, hormone signaling pathways, and leaf senescence. Additionally, WRKY, ERF, and NAC transcription factors were found to be enriched among the significant DEGs. Notably, eugenol and clove essential oil treatments, as well as exposure to heat stress, were associated with the regulation of leaf senescence. Furthermore, the application of eugenol and clove essential oil mitigated the heat-induced reductions in the contents of chlorophyll a, chlorophyll b, and total chlorophyll in Chinese cabbage. In summary, our findings suggest that eugenol and clove essential oil effectively enhance thermotolerance in Chinese cabbage by modulating leaf senescence and hormone responses.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100324"},"PeriodicalIF":5.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000069/pdfft?md5=74c8fcd3ce5ec84779ef252c11477d02&pid=1-s2.0-S2214662824000069-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"More than colorful: phosphorus allocation to major chemical fractions shifts during leaf development in species exhibiting delayed greening","authors":"Li Yan ,&nbsp;Xiang-Wen Fang ,&nbsp;Wei Wang ,&nbsp;Dan Tang ,&nbsp;Hans Lambers","doi":"10.1016/j.cpb.2024.100323","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100323","url":null,"abstract":"<div><p>The colorful “delayed leaf greening” is a common but overlooked phenomenon in phosphorus (P)-limited environments in habitats in tropical, subtropical and temperate forests, but the physiological mechanism underpinning it remains unclear. It is important to understand how allocation of phosphorus to major leaf P fractions shifts during leaf development, as a strategy for utilizing P efficiently. We measured concentrations of leaf nitrogen and P and five chemical P fractions, and eight leaf chemical element concentrations (K, Ca, Mg, Mn, Fe, Cu, Zn and Se) in young and mature leaves of six woody plants exhibiting delayed greening in China. We also measured leaf mass per area, photosynthetic rate, photosynthetic phosphorus-use efficiency, and soil nutrient concentrations. The results indicate six species exhibiting delayed greening had different leaf P concentrations during leaf development, but the same nitrogen concentrations. We further show major leaf chemical P fractions like metabolite P, nucleic acid P and lipid P showed differences in young and mature leaves. The concentration of lipid P, nucleic acid P, Pi and residual P significantly decreased from young to mature leaves, while that of metabolite P was constant. There was a greater allocation of P to phospholipids and metabolite P in mature leaves. The concentration of Cu and K were significantly higher in young leaves. This study provides new insight to investigate the roles of different P fractions in young and mature leaves, and how the allocation shifts for plants to utilize phosphorus.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100323"},"PeriodicalIF":5.4,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000057/pdfft?md5=678660152f1b2ad0fb0d411b52389d0d&pid=1-s2.0-S2214662824000057-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139550099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants","authors":"L.M. Gatica-Soria ,&nbsp;M.V. Canal ,&nbsp;M.E. Roulet ,&nbsp;H. Sato ,&nbsp;V. Gómez Villafañe ,&nbsp;E. Welchen ,&nbsp;M.V. Sanchez-Puerta","doi":"10.1016/j.cpb.2024.100322","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100322","url":null,"abstract":"<div><p>Mitonuclear incompatibility results from a breakdown of the coordinated function between co-evolved genes located in nuclear and mitochondrial compartments. Horizontal Gene Transfer (HGT), involving the acquisition of genes from unrelated species, can trigger mitonuclear incompatibilities when foreign gene products interact with native ones, particularly in multisubunit complexes. Recent findings highlighted rampant HGT in the mitochondrial genomes of holoparasitic plants of the genus <em>Lophophytum</em> (Balanophoraceae). In <em>Lophophytum</em>, some mitochondrial genes involved in the Oxidative Phosphorylation (OXPHOS) system were acquired from their legume hosts, unlike the nuclear-encoded OXPHOS subunits, which remain native. This unique configuration of a doubly chimeric OXPHOS, combining native nuclear-encoded subunits with both foreign and native mitochondrial-encoded subunits, raises questions regarding the potential effects of the interactions between native and foreign proteins on mitochondrial respiration activity in <em>Lophophytum</em>. We examined the mitochondrial ultrastructure, evaluated protein expression via Western blots, and analyzed cellular respiration through oxygen consumption rates and adenylate content in these holoparasitic plants. Surprisingly, our results revealed no disruption of the OXPHOS machinery or activity in <em>Lophophytum</em> despite the functional replacement of several native protein subunits by foreign homologs. Furthermore, there was no apparent impact on the OXPHOS system given their parasitic lifestyle and complete loss of photosynthesis.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100322"},"PeriodicalIF":5.4,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000045/pdfft?md5=f7b5b9d4c132842a8f160c297e7500f1&pid=1-s2.0-S2214662824000045-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139550098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus siamensis strain BW enhances rice growth and salinity tolerance through redox equilibrium and hormone modulation","authors":"Brahim Oubaha ,&nbsp;Ray Singh Rathore ,&nbsp;Jayram Bagri ,&nbsp;Nitin Kumar Singhal ,&nbsp;Koushik Mazumdar ,&nbsp;Vikas Rishi ,&nbsp;Ashwani Pareek ,&nbsp;Sneh Lata Singla-Pareek","doi":"10.1016/j.cpb.2024.100321","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100321","url":null,"abstract":"<div><p>High soil salinity has an unfavorable consequence on the growth and productivity of rice crop. However, some salt-tolerant plant growth-promoting bacteria (ST-PGPB) regulate specific physiological, biochemical, and molecular properties to promote crop growth while minimizing the detrimental effects of salt stress. In this regard, we isolated ST-PGPB from rhizospheric soil and examined it to mitigate the salinity stress in rice seedlings. The growth of the bacterium at 3 M NaCl demonstrated its halotolerance, and 16S rRNA sequencing identified it as <em>Bacillus siamensis</em>, and the isolated strain was named BW. Further study indicated that biopriming with BW strain helps plant growth promotion-related phenotype and significantly mitigates salinity stress in rice seedlings. Treatment of rice seeds with BW resulted in significantly improved germination of seedlings at 75 mM to 150 mM NaCl, along with better physiology and biochemical parameters than the untreated ones. Furthermore, <em>Bacillus</em> sp. BW efficiently colonizes rice roots and produces auxin and siderophore, <em>via</em> forming biofilm under different salt concentrations. Under 100–200 mM NaCl treatment conditions, the extracellular metabolite profile from BW showed a substantial abundance in specific metabolites, such as osmoprotective chemicals, suggesting the likely protective mechanism against salinity stress damage. This study demonstrates the role and potential of a halotolerant- BW strain in supporting the growth of rice plants under salinity conditions.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100321"},"PeriodicalIF":5.4,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000033/pdfft?md5=2b12f8098d5ab6e8ff04cee502dd8400&pid=1-s2.0-S2214662824000033-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of castor pistillate lines and analysis of mode of inheritance for resistance to Fusarium wilt disease in castor (Ricinus communis L.)","authors":"C. Deepika ,&nbsp;S.R. Venkatachalam ,&nbsp;A. Yuvaraja ,&nbsp;P. Arutchenthil ,&nbsp;N. Indra ,&nbsp;V. Ravichandran ,&nbsp;P. Veeramani ,&nbsp;P. Kathirvelan","doi":"10.1016/j.cpb.2024.100319","DOIUrl":"https://doi.org/10.1016/j.cpb.2024.100319","url":null,"abstract":"<div><p>Castor is an industrially economic and valuable oilseed crop cultivated worldwide. There is a constant upsurge in demand for its oil. But wilt caused by <em>Fusarium spp.</em> is a devastating disease that severely affects the productivity depending upon the crop stage. Stable high yielding pistillate lines serve as donors in heterosis breeding programme and for further biotechnological advancements. Genetic characterization of wilt resistance indicated the role of duplicate dominant epistasis in YTP 1 × TMV 5, complementary epistasis in DPC 9 × JP 65 and JP 65 × SKI 215, duplicate recessive epistasis in YRCP 1 × DPC 9. Monogenic recessive nature of wilt resistance was reported in other four cross combinations <em>viz.,</em> YRCP 2 × JP 65, SKP 84 × JP 65, YRCP 2 × DPC 9 and YRCP 2 × SKP 84. Magnified images taken using LED phase contrast microscope portrayed the presence of microconidia and macroconidia and Scanning Electron Microscope (SEM) image analysis showed the presence of intact internal cell structures in resistant check (48−1) while the cell structures were disturbed with mycelial growth in the susceptible check (JI 35). Among 21 pistillate lines screened, seven <em>viz.,</em> DPC 9, DPC 16, SKP 84, JP 96, GEETA, M 574 and M 619–1 were resistant. By screening P1, P2, F1, F2, BC₁F₁ (P1) and BC₁F₁ (P2) generations of eight crosses under field and pot test method, the stable pistillate line DPC 9 was found to be wilt resistant. F₁ generation plants expressed 100% susceptibility indicating the recessive nature of wilt resistance. JP 65 × SKI 215 and YRCP 1 × DPC 9 showed the minimum incidence comparing other F₂ populations. The backcross (YRCP 1 × DPC 9) × DPC 9 was found to possess the lowest wilt incidence compared to other populations under field and green house condition. Hence the identified lines could be better used to develop wilt resistant high yielding hybrid and for further identifying and introgressing genomic regions conferring wilt resistance to high yielding popular variety through linkage/QTL mapping technique.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100319"},"PeriodicalIF":5.4,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221466282400001X/pdfft?md5=c115dcb85a0496f6a6bd8a0b3dca03c3&pid=1-s2.0-S221466282400001X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139494122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}