GbbZIP41转录因子参与银杏萜类三内酯生物合成。

IF 4.5 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2025-01-31 DOI:10.1016/j.cpb.2025.100449

Nuo Wang, Yi Tu, Sirui Zeng, Jiabao Ye, Weiwei Zhang, Feng Xu, Yongling Liao

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引用次数: 0

摘要

三内酯萜（Terpene trilactones, TTLs）是银杏树（ginkgo biloba）中重要的次生代谢产物，在治疗人类心血管疾病和预防血栓形成中起着至关重要的作用，在食品、医药和保健品中有着广泛的应用。然而，关于TTLs生物合成的转录调控研究较少，TTLs生物合成的基因调控网络也不清楚。本文筛选了可能参与TTLs生物合成的转录因子GbbZIP41，并通过转基因技术验证了其功能。结果表明，GbbZIP41是一个位于细胞核的蛋白，编码477个氨基酸，在双叶中高表达。结果表明，双叶总萜含量和GbbZIP41基因表达呈相反趋势。此外，烟草中GbbZIP41基因的过表达降低了萜类化合物的含量，下调了MVA途径和MEP途径的酶基因。因此，这些结果表明GbbZIP41通过抑制TTLs合成途径中的关键酶基因负调控TTLs的生物合成。

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The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L.

Terpene trilactones (TTLs) are important secondary metabolites in ginkgo tree (Ginkgo biloba), which play a crucial role in the treatment of human cardiovascular disease and prevention of thrombosis, and have been widely used in food, medicine and health products. However, there are few studies on the transcriptional modulation of TTLs biosynthesis and gene regulatory network of TTLs biosynthesis remains unclear. Here, we screened the transcription factor GbbZIP41 that may be involved in the biosynthesis of TTLs and verified its function by transgenic technology. The results showed that GbbZIP41 is a protein located in the nucleus and encodes 477 amino acids, which is highly expressed in G. biloba leaves. The analysis of total terpene content and GbbZIP41 gene expression in G. biloba leaves showed the opposite trend. In addition, the overexpression of GbbZIP41 gene in tobacco reduced the content of terpenoids and down-regulated the enzyme genes of MVA pathway and MEP pathway. Therefore, these results suggested that GbbZIP41 negatively regulates the biosynthesis of TTLs by inhibiting key enzyme genes in the TTLs synthesis pathway.

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.