Current Plant Biology最新文献

{"title":"Genome-wide sequence comparison and development of InDel and SNP markers to facilitate localized rice breeding","authors":"Juan Pariasca-Tanaka ,&nbsp;Yoshiaki Ueda ,&nbsp;Katsuhiko Kondo ,&nbsp;M. Asaduzzaman Prodhan ,&nbsp;Toavintsoa Rajonandraina ,&nbsp;Harisoa Nicole Ranaivo ,&nbsp;Mbolatantely Fahazavana Rakotondramanana ,&nbsp;Hiroki Saito ,&nbsp;Lam Thi Dinh ,&nbsp;Matthias Wissuwa","doi":"10.1016/j.cpb.2025.100469","DOIUrl":"10.1016/j.cpb.2025.100469","url":null,"abstract":"<div><div>Rice (<em>Oryza sativa</em> L.) is the staple crop for over half of the world's population and selection in different environmental and climatic conditions has led to the development of numerous local rice cultivars. In a changing environment, these local cultivars may need to be adapted to emerging stresses. Whole-genome sequences are available only for a small fraction of existing rice cultivars, limiting the opportunity for genomics-based marker-assisted selection (MAS) in most local cultivars. To efficiently develop locally-adapted rice cultivars resilient to adverse environmental conditions, our objective was to develop a pipeline for the analysis of re-sequencing data and the development of low-cost allele-specific markers that can be implemented in developing countries. This study focused on three rice cultivars (X265, F160, and Nerica4) commonly grown in Madagascar. We provide whole-genome sequencing data and identify sequence variations compared to publicly available sequences, followed by the development of simple PCR-based InDel and SNP markers. Their effectiveness in distinguishing different alleles at the <em>Ghd7</em> locus controlling heading date was demonstrated, enabling us to employ these markers for MAS in a breeding population developed between X265 and a donor “Liu He Xi He”. In addition, we developed PCR-based SNP markers for the same population on <em>qLFT-5</em>, a quantitative trait locus for low-fertility tolerance in which the donor allele increases total panicle weight under low-input conditions. This study presents a practical pipeline for the utilization of next generation sequencing-derived large-scale data to accelerate low-cost marker development for MAS in rice and other crop species.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100469"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug target screening for Rheumatoid Arthritis by Curcuma caesia through computational approach","authors":"Ankita Pati ,&nbsp;Mahendra Gaur ,&nbsp;Atmaja Sahu ,&nbsp;Bharat Bhusan Subudhi ,&nbsp;Dattatreya Kar ,&nbsp;Jyoti Ranjan Parida ,&nbsp;Ananya Kuanar","doi":"10.1016/j.cpb.2025.100468","DOIUrl":"10.1016/j.cpb.2025.100468","url":null,"abstract":"<div><div><em>Curcuma caesia</em> has been a subject of inflammatory and autoimmune disease research, showing promising anti-inflammatory properties. The present research aims to investigate the anti-rheumatic potential of the rhizome through network pharmacology, molecular docking and molecular dynamic simulations approaches. Phytocompounds were retrieved from PubChem, and their targets were predicted using Swiss target prediction, SEA, SuperPred, and BindingDB. The 13 phytocompounds overlapping with its 41 predicted proteins and its related pathways generated a Cytoscape interaction network revealing that <em>C. caesia</em> may inhibit rheumatoid arthritis through different metabolic pathways. NFKB1, PRKCA, RAC1, STAT3, and TLR4 were identified as potential core targets while 13 compounds α-Terpineol, Ar-tumerone, 3,3,8,8-tetramethyl-tricyclo[5.1.0.0(2,4)] oct-5-ene-5-propanoic acid (TPA), Rosifoliol, 2-Nonanone, Terpinen-4-ol, Dihydrocarveol, 5-Nonanone, Camphene, Linalool, Bornyl acetate, Camphor were identified as potential core compounds. Molecular docking and Induced Fit Docking (IFD) analysis revealed that NFKB1, PRKCA, and RAC1, along with the newly discovered TPA compound, are the most significant targets and bioactive compounds, respectively. Furthermore, in interactions such as TPA-RAC1, TPA might be a potential \"chelating ligand\" and may play a role in lowering concentrations of metal in blood. In addition, the molecular dynamics simulation (MDS) studies for 200 ns elucidated the binding mechanism of TPA with NFKB1, PRKCA and RAC1. In conclusion, TPA has a promising inhibiting potential against Rheumatoid Arthritis and thus necessitates further validation through <em>in vitro</em> and <em>in vivo</em> experiments.Therefore, the present study revealed the main mechanisms behind the anti-rheumatic effects of <em>C. caesia</em>, paving the path for further research on these compounds.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100468"},"PeriodicalIF":5.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From omics to orchard: The role of omics in durian cultivation","authors":"Pinnapat Pinsorn ,&nbsp;Supaart Sirikantaramas","doi":"10.1016/j.cpb.2025.100466","DOIUrl":"10.1016/j.cpb.2025.100466","url":null,"abstract":"<div><div>Durian (<em>Durio zibethinus</em>) is a significant fruit in Southeast Asia, with major exporters including Thailand and Malaysia, and expanding production in other countries (e.g., Indonesia, the Philippines, and Vietnam. Global demand for durian has increased over the past decade. However, cultivation and production face challenges due to climate change, disease outbreaks, and pathogen resistance to fungicides, leading to yield losses and high orchard maintenance costs. Omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, offer advanced tools for understanding the molecular mechanisms underlying biological processes and could greatly benefit durian cultivation. By applying these technologies, scientists and orchardists can gain valuable insights into key traits such as disease resistance, fruit quality, and ripening. However, durian-related omics research remains relatively limited. In this review, we aim to broaden the understanding of these topics and discuss successful omics applications in other fruit orchards, highlighting similarities that could be applied to durian cultivation. These insights provide a roadmap for integrating omics into durian breeding programs and orchard management. In the future, we anticipate that ongoing durian research, advancements in omics technologies, and reduced study-related costs will lead to more efficient and sustainable durian production, ultimately benefiting both orchardists and consumers by improving yield and quality traits.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100466"},"PeriodicalIF":5.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brassinosteroids in maize: Biosynthesis, signaling pathways, and impacts on agronomic traits","authors":"Jingjie Zhang ,&nbsp;Shiyi Wu ,&nbsp;Miao Wang ,&nbsp;Jinke Chang ,&nbsp;Xiaopeng Li","doi":"10.1016/j.cpb.2025.100465","DOIUrl":"10.1016/j.cpb.2025.100465","url":null,"abstract":"<div><div>Maize is one of the most widely cultivated crops for producing human food, animal feed, and ethanol biofuel. Recent studies have indicated that brassinosteroids (BRs), essential phytohormones for plant growth, development and stress adaptation across plant species, significantly regulate several maize agronomic traits, such as plant height, leaf angle, reproductive development and kernel size. So far, most previous reviews have mainly focused on discussing molecular functions of BRs in model plants like Arabidopsis and rice. A detailed summary of BRs in maize, however, has not yet been presented. In this review, we provide a comprehensive overview of the biosynthesis and signaling transduction pathways of BRs, and summarize the roles of BRs in regulating some agronomic traits such as plant architecture and kernel yield in maize. We also discuss potential opportunities and challenges associated with leveraging BR-centered molecular design strategies for maize improvement.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100465"},"PeriodicalIF":5.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing reveals transcriptional regulation and metabolic pathways of terpenoid biosynthesis in developing Cinnamomum camphora leaf cells","authors":"Zheng Qin ,&nbsp;Caihui Chen ,&nbsp;Ting Zhang ,&nbsp;Yanfang Wu ,&nbsp;Yongjie Zheng","doi":"10.1016/j.cpb.2025.100467","DOIUrl":"10.1016/j.cpb.2025.100467","url":null,"abstract":"<div><div><em>Cinnamomum camphora</em> (Camphor tree) is an economically significant species known for its terpenoid-rich essential oils. However, the molecular mechanisms underlying its leaf development remain poorly understood, especially at the cellular level. In this study, we applied high-throughput single-cell RNA sequencing (scRNA-seq) to profile the transcriptomic landscape of developing <em>C. camphora</em> leaves, identifying eight distinct cell populations, including mesophyll, epidermal, guard, vascular, and proliferating cells. Pseudotime trajectory analysis revealed the dynamic progression of mesophyll cell differentiation, with three distinct developmental states observed as cells transitioned from proliferating to differentiated stages. We identified key metabolic pathways involved in terpenoid biosynthesis, lipid metabolism, and photosynthesis. Notably, genes such as <em>4CLL9</em>, <em>CHLP</em> or <em>GPPS1</em> showed cell-type-specific expression in mesophyll or epidermal cells. Additionally, transcription factors from the MYB and bHLH families were enriched in specific cell types, regulating secondary metabolism and hormone signaling. This study not only provides a detailed transcriptomic atlas of <em>C. camphora</em> leaf development but also uncovers novel cell-type-specific marker genes, key regulatory networks, and metabolic pathways, offering valuable resources for future investigations into terpenoid metabolism and cellular differentiation in woody species.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100467"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antarctic fungal inoculation enhances drought tolerance and modulates fruit physiology in blueberry plants","authors":"Cristian Balbontín ,&nbsp;Sebastián Flores ,&nbsp;Marisol Reyes ,&nbsp;Victoria Urrutia ,&nbsp;Carolina Parra-Palma ,&nbsp;Luis Morales-Quintana ,&nbsp;Patricio Ramos","doi":"10.1016/j.cpb.2025.100462","DOIUrl":"10.1016/j.cpb.2025.100462","url":null,"abstract":"<div><div>Climate change represents a direct threat to global food security, which includes prolonged droughts caused by global warming adversely affecting agricultural crop development and yield. Symbiotic associations between plants and extremophilic microorganisms have been shown to play a crucial role in enhancing plant adaptation to environmental stress. In this study, ‘Legacy’ blueberry plants were inoculated with two endophytic fungi, <em>Penicillium chrysogenum</em> and <em>Penicillium brevicompactum</em>, isolated from Antarctic plants, to evaluate their effects on fruit productions and plant responses, to water stress. The assays were conducted under drought conditions to simulate climate change, assessing the physiological and biochemical responses of fruits from inoculated and non-inoculated plants. Results indicated that inoculated plants exhibited an improvement in the physiological responses of plants under drought stress. The inoculated plants (W-E + ) consistently perform better than non-inoculated plants (W-E-) under water stress, particularly in water potential, PSII efficiency, and photosynthetic function. Meanwhile, the fruits obtained from these plants did not show differences in fruit size, while the weight, SSC/TA and firmness were greater in the inoculated fruits compared to the non-inoculated plants under drought stress. Additionally, the fruits showed a reduction in total phenolic and flavonoid content during stress periods, while enzymatic activities of superoxide dismutase and peroxidase were enhanced under the same conditions. These findings suggest that functional symbiosis with Antarctic microorganisms may alleviate drought-induced stress in plants by modulating their biochemical activities compared to non-inoculated counterparts.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100462"},"PeriodicalIF":5.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive allelic series analysis uncovers the novel function of the tomato FALSIFLORA gene in the cessation of floral meristem activity","authors":"Abraham S. Quevedo-Colmena ,&nbsp;Wim H. Vriezen ,&nbsp;Pieter G.A. Wesselink ,&nbsp;José M. Pérez-Jiménez ,&nbsp;Benito Pineda ,&nbsp;Begoña García-Sogo ,&nbsp;Trinidad Angosto ,&nbsp;Vicente Moreno ,&nbsp;Fernando J. Yuste-Lisbona ,&nbsp;Rafael Lozano","doi":"10.1016/j.cpb.2025.100461","DOIUrl":"10.1016/j.cpb.2025.100461","url":null,"abstract":"<div><div>Plants undergo continuous growth thanks to meristems, specialized groups of pluripotent stem cells that remain undifferentiated throughout the plant's life. Meristem transition from the vegetative to the reproductive phase heavily influences plant reproductive success and agricultural productivity. In tomato (<em>Solanum lycopersicum</em> L.), <em>FALSIFLORA</em> (<em>FA</em>), the orthologue of the Arabidopsis <em>LEAFY</em> gene, promotes floral transition by specifying floral meristem identity and regulating the expression of genes responsible for floral organ identity and development. This study expanded the <em>FA</em> allelic series by combining the screening of an EMS mutant collection with overexpression, silencing and CRISPR/Cas9 genome editing approaches, aimed to deepen the understanding of the functional role of <em>FA</em> during reproductive development. The phenotypic and molecular characterization of the <em>FA</em> allelic series revealed its multifaceted role in both early and late stages of floral ontogeny. Besides promoting floral transition and specifying floral meristem identity, <em>FA</em> also plays a role in inflorescence meristem maturation and termination, thereby regulating the inflorescence architecture. Furthermore, <em>FA</em> potentially exerts regulatory control over the expression of the <em>AGAMOUS</em> homolog (<em>TOMATO AGAMOUS1</em>, <em>TAG1</em>), which in turn may contribute to the deregulation of <em>WUSCHEL</em> (<em>SlWUS</em>) during floral development, underscoring its function in promoting carpel development and suppressing floral stem cell activity, thereby establishing floral determinacy. Our findings reveal for the first time the novel role of <em>FA</em> in the cessation of floral meristem activity in tomato, and demonstrate the value of mutant allelic series as powerful tools for elucidating gene functions and understanding the intricate molecular basis underlying biological processes.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100461"},"PeriodicalIF":5.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genome-wide expression analysis of NLP transcription factors elucidates their crucial role in enhancing nitrogen response in wheat (Triticum aestivum L.)","authors":"Shefali Mishra,&nbsp;Garima Singroha,&nbsp;Ratan Tiwari,&nbsp;Pradeep Sharma","doi":"10.1016/j.cpb.2025.100463","DOIUrl":"10.1016/j.cpb.2025.100463","url":null,"abstract":"<div><div>Plant specific transcription family NLP (NIN-like protein) represent a gene family essential for regulating plant physiological processes, particularly growth and nitrate-nitrogen response. This study investigated the characteristics and expression profiles of NLP genes in wheat. Thephylogenetic analysis, grouped 18 NLP found in the wheat genome into three clades. TaNLP genes share collinear relationships with rice and Arabidopsis NLPs as evidenced in comparative genomic analysis. Segmental duplications within the wheat genome is primarily responsible for the TaNLP family's expansion. In Protein network analysis TaNLP4 was identified as a hub gene, interacting with multiple other genes to coordinate nitrogen responses. Both low and optimal nitrate conditions were used to measure nitrate uptake in wheat roots and the expression patterns of <em>TaNLP</em> genes under these conditions. During the seedling stage, tissue-specific expression analysis revealed that close homologs of AtNLP7 such as TaNLP4 and TaNLP5 are highly expressed in both roots and leaves while <em>TaNLP17</em> and <em>TaNLP18</em> displayed preferential expression in roots. Additionally, TaNLP2 in root tissue was upregulated under low nitrate conditions. Most TaNLPs are predominantly expressed in leaves and showed positive responses to nitrate treatments. These findings highlight the characteristics properties and biological roles of <em>TaNLP</em> genes in wheat, offering potential insights into improving nitrogen use efficiency for sustainable wheat production without use of excessive fertilizers.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100463"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twenty years of AT-HOOK MOTIF NUCLEAR LOCALIZED (AHL) gene family research – Their potential in crop improvement","authors":"Michaela Martinčová,&nbsp;Aleš Soukup","doi":"10.1016/j.cpb.2025.100460","DOIUrl":"10.1016/j.cpb.2025.100460","url":null,"abstract":"<div><div>The <em>AT-HOOK MOTIF NUCLEAR LOCALIZED</em> (<em>AHL</em>) gene family encodes transcriptional regulators that are able to bind to AT-rich DNA regions, remodel chromatin architecture and mediate epigenetic and transcriptional target gene expression. They play critical roles in plant development such as regulation of organ morphogenesis, meristem activity, flowering timing or hypocotyl elongation. Moreover, <em>AHLs</em> are involved in plant biotic and abiotic stress responses such as drought, salinity and pathogen resistance through the stress responsive gene networks and hormone signalling. Their multilevel effect on gene expression and pleiotropic response position <em>AHLs</em> as key integrators of developmental and environmental cues. Recent experimental results have indicated potential agricultural applications of <em>AHLs</em>, including enhancing crop resilience to climate change-induced stresses and optimizing growth traits. Harnessing the functional diversity of <em>AHLs</em> could offer innovative strategies for sustainable agriculture, with targeted manipulation of these genes promising improved yield, stress tolerance, and adaptability in various crop species.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100460"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic, transcriptional, and hormonal responses of Panax ginseng C. A. Meyer to nitrogen deficiency","authors":"Hao Liang ,&nbsp;Hai Sun ,&nbsp;Cai Shao ,&nbsp;Bochen Lv ,&nbsp;Jiapeng Zhu ,&nbsp;Weiyu Cao ,&nbsp;Jixin Zhou ,&nbsp;Yayu Zhang","doi":"10.1016/j.cpb.2025.100447","DOIUrl":"10.1016/j.cpb.2025.100447","url":null,"abstract":"<div><div>Nitrogen (N), a key macronutrient, plays a pivotal role in modulating plant growth, development, and the synthesis of secondary metabolites. Understanding the response of medicinal plants to N deficiency is critical for optimizing their quality. However, an integrated analysis of the metabolome, transcriptome, and hormone profiles associated with N deficiency in <em>Panax ginseng</em> has not been previously conducted. In this study, the effects of N deprivation on <em>Panax ginseng</em> seedlings were investigated through comprehensive metabolic, transcriptional, and hormonal analyses. N deficiency led to an increased accumulation of nucleotides, flavonoids, phenolic acids, lipids, alkaloids, lignans, coumarins, amino acids, and their derivatives. In contrast, the content of terpenoids was significantly reduced. Additionally, alterations in the levels of auxin (IAA), cytokinin (CTK), gibberellin (GA), and jasmonic acid (JA) were observed under N deprivation. Transcriptomic analysis revealed downregulation of farnesyl diphosphate synthase (<em>FPS</em>) and farnesol kinase (<em>FOLK</em>), alongside upregulation of ten Cytochrome P450 (<em>CYP450</em>) genes involved in terpenoid biosynthesis. Furthermore, genes associated with IAA and CTK signaling pathways were downregulated, while genes related to GA and JA signaling were upregulated. Exogenous CTK application under N deficiency resulted in elevated levels of several terpenoid metabolites, including Oleanolic acid-3-o-Glucosyl (1→2)glucoside, 3-oxo-9,19-cyclolanost-24-en-26-Oic acid, Majoroside R1, 3-Oxoolean-12-en-28-oic Acid, Pseudoginsenoside RT5, 3-Hydroxyurs-12-en-28-oic acid, Oleanolic acid-3-o-[xylosyl(1→2)-Arabinosyl(1→6)]glucoside, and 24,30-dihydroxy-12(13)-ene-Lupeol. These results suggest that exogenous CTK can enhance the accumulation of terpenoid metabolites under N-deficient conditions in <em>Panax ginseng</em>. This study provides valuable insights into the molecular mechanisms underlying N regulation in <em>Panax ginseng</em> and offers new directions for nutrient management strategies in the ecological cultivation of this plant.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100447"},"PeriodicalIF":5.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}