Current Plant Biology最新文献

{"title":"Integrated QTL mapping and transcriptomic profiling elucidate molecular determinants of sucrose accumulation in apricot (Prunus armeniaca L)","authors":"Fengchao Jiang ,&nbsp;Li Yang ,&nbsp;Junhuan Zhang ,&nbsp;Meiling Zhang ,&nbsp;Wenjian Yu ,&nbsp;Haoyuan Sun","doi":"10.1016/j.cpb.2025.100500","DOIUrl":"10.1016/j.cpb.2025.100500","url":null,"abstract":"<div><div>Apricot is a commercially vital stone fruit prized for its distinctive organoleptic characteristics and nutritional value. We developed an F1 population from ‘Chuanzhihong’ × ‘Luotuohuang’ to investigate the genetic basis of sugar metabolism. Whole-genome resequencing identified 63,162 high-quality InDel markers, while metabolomic profiling quantified soluble sugars (sucrose, glucose, fructose) across fruit developmental stages. Continuous variation in sugar accumulation patterns indicated polygenic inheritance. A high-density genetic map (601.5 cM, 0.44 cM average interval) revealed 20 stable QTLs across 8 linkage groups, explaining 8.0–16.2 % phenotypic variance for individual and total sugars. WGCNA identified sugar component-specific modules showing strong correlations (r = 0.57–0.95). Integrative analysis prioritized <em>PA08G27233</em> as a hub gene within QTL intervals, encoding a SWEET transporter (designated <em>PaSWEET1</em>) with evolutionary conservation to AtSWEET1. This study elucidates molecular mechanisms of photoassimilate partitioning in apricot and provides genomic resources (high-resolution map, candidate genes) for marker-assisted breeding. The findings advance functional characterization of sugar metabolism pathways in <em>Prunus</em> species.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"43 ","pages":"Article 100500"},"PeriodicalIF":5.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The PROSPECT model in high-throughput phenotyping for peanut leaf parameter estimation: Comparative performance of hyperspectral inversion models","authors":"Xue Kong ,&nbsp;Jiangtao Zhao ,&nbsp;Yirou Liu ,&nbsp;Bo Bai ,&nbsp;Juntao Yang ,&nbsp;Yangyang Fan ,&nbsp;Guowei Li ,&nbsp;Zhenhai Li ,&nbsp;Shubo Wan","doi":"10.1016/j.cpb.2025.100498","DOIUrl":"10.1016/j.cpb.2025.100498","url":null,"abstract":"<div><div>Accurate estimation of leaf biochemical parameters is crucial for understanding crop physiology and monitoring nutritional status. Remote sensing algorithms perform well on limited germplasm, but the transferability to high-throughput phenotyping with diverse genotypes remains unclear. This study estimated leaf chlorophyll content (Cab), equivalent water thickness (Cw), and dry matter content (Cm) using the single vegetation index (SVI), random forest (RF), and the PROSPECT model to evaluate the performance and transferability of these models under diverse peanut germplasm conditions. Results showed that Transformed Chlorophyll Absorption in Reflectance Index (TCARI), Water Index (WI), and Modified Simple Ratio (mSR) were strongly correlated with Cab, Cw, and Cm, respectively, highlighting their importance in the inversion models. Comparative analysis revealed that the RF model achieved the highest accuracy for Cab (R² = 0.77, RMSE = 8.14 µg cm⁻²), Cw (R² = 0.67, RMSE = 1.1 × 10⁻³ g cm⁻²), and Cm (R² = 0.50, RMSE = 6.2 × 10⁻⁴ g cm⁻²), followed by the PROSPECT model, with R² and RMSE of 0.76 and 8.21 µg cm⁻² for Cab, 0.61 and 1.2 × 10⁻³ g cm⁻² for Cw, and 0.38 and 7.7 × 10⁻⁴ g cm⁻² for Cm, respectively. However, the PROSPECT model was most effective in Cab inversion across diverse germplasm resources (R² = 0.58, RMSE = 7.68 µg cm⁻²), demonstrating its superior transferability and stability. These results underscore its value in high-throughput phenotyping and improving the accuracy and generalizability of crop biochemical parameter estimation.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"43 ","pages":"Article 100498"},"PeriodicalIF":5.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analyses reveal the differentiation of biochemical constituents from fresh and dry Andrographis paniculata-derived vesicle-like nanoparticles","authors":"Qian Wu ,&nbsp;Chunyu Wu ,&nbsp;Ziying Wu ,&nbsp;Shibo Chen ,&nbsp;Hong-Bin Wang ,&nbsp;Hong-Lei Jin ,&nbsp;Kewei Zhao","doi":"10.1016/j.cpb.2025.100497","DOIUrl":"10.1016/j.cpb.2025.100497","url":null,"abstract":"<div><div>Plant-derived vesicle-like nanoparticles (PDVLNs) contain various biomolecules and metabolites that aid in material conversion, signal transduction, cross-species information communication, and gene expression regulation. Despite their potential, there have been no prior reports on <em>Andrographis paniculata</em>-derived vesicle-like nanoparticles (ApDVLNs) and their biological components. In this study, ApDVLNs were identified through physical and biochemical characterization, and their cargoes were analyzed using multi-omics (microRNA omics, proteomics, non-targeted metabolomics). Joint analysis revealed that both fresh and dry ApDVLNs were rich in miRNAs involved in regulating plant growth, development, and environmental adaptation. Target genes of specific miRNAs in fresh ApDVLNs were related to photosynthesis and hormone signal transduction, at the same time, those in dry ApDVLNs were involved in secondary metabolite accumulation and nucleic acid repair. miRNAs like miR396-x and miR166-y may also regulate infections, cancer, cardiovascular diseases, immune disorders, and metabolic diseases in <em>Homo sapiens</em>. Both fresh and dry ApDVLNs contained numerous proteins primarily associated with catalytic activity, binding, transporter activity, and other functions. They included proteins involved in endocytosis, ABC transporters, vesicles, aquaporins, tetraspanins, and membrane proteins, which support ApDVLNs functionality. Interestingly, many miRNAs and proteins were present in both fresh and dry ApDVLNs, but dry ApDVLNs contain more of these miRNAs and proteins. Additionally, dry ApDVLNs were metabolite-rich, identifying 23,760 metabolites, with 53.06 % of the annotated ones belonging to lipids and lipid-like molecules. The results of these works demonstrate that ApDVLNs are abundant in miRNAs, proteins and metabolites. Enhancing the concentration of these substances during the drying process is crucial for their biological function. These components form the material basis for their biological activities.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"43 ","pages":"Article 100497"},"PeriodicalIF":5.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue infiltration of polyethylene, polypropylene, and polystyrene microplastics in Solanum tuberosum L. influences plant growth and yield","authors":"Zonaira Qaiser ,&nbsp;Noreen Khalid ,&nbsp;Adeel Mahmood ,&nbsp;Shiou Yih Lee ,&nbsp;Zarrin Fatima Rizvi ,&nbsp;Muhammad Kashif Irshad ,&nbsp;Ujala Ejaz ,&nbsp;Muhammad Aqeel","doi":"10.1016/j.cpb.2025.100496","DOIUrl":"10.1016/j.cpb.2025.100496","url":null,"abstract":"<div><div>This study was carried out to evaluate the interaction between terrestrial food crop plants and microplastics (MPs) with a focus on understanding their uptake, effects on growth, physiological, biochemical, and yield characteristics of two different cultivars of <em>Solanum tuberosum</em> L. i.e., Variety-1, Astrix (AL-4) and Variety-2, Harmes (WA-4). Polyethylene (PE), polystyrene (PS), and polypropylene (PP) spheres of size 5 μm were applied to the soil at concentrations of 0 %, 1 %, and 5 %. Morphological parameters, including seed germination rate, shoot and root lengths, leaf area, and fresh and dry biomass of plants, got reduced significantly with the increase in MP concentration. PS MPs caused the most negative impact, particularly at 5 %, leading to the greatest decline in growth and Na, Mg, Zn, Cu, Ni, and Mn nutrient content. The highest DPPH scavenging activity was observed in the 5 % PS MPs treatment with approximately a 45.34 % increase from the control, indicating its potential to enhance antioxidant activity in response to stress caused by PS MPs. Both reducing and non-reducing sugar contents and total proteins were also decreased significantly. Vitamin C content exhibited a significant increase in response to MPs, with the highest levels recorded under 5 % PS MPs treatments. This suggests an adaptive antioxidant response to mitigate oxidative damage induced by MPs. SEM analysis revealed tissue infiltration of MP particles in shoots, leaves, and tubers of both varieties. Among MPs, PS had the most detrimental effects, followed by PP and PE, with higher concentrations increasing the negative impact.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"43 ","pages":"Article 100496"},"PeriodicalIF":5.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive transcriptomic and metabolomic analyses reveal developmental changes in one-year-old and two-year-old Polygonatum cyrtonema hua","authors":"Liang Wu ,&nbsp;Yuqin Qiu ,&nbsp;Yuting Fang ,&nbsp;Can Huang ,&nbsp;Dawei Song ,&nbsp;Duohui Li ,&nbsp;Li Zhao ,&nbsp;Haiyang Zhao ,&nbsp;Lamei Qi ,&nbsp;Lishang Dai","doi":"10.1016/j.cpb.2025.100495","DOIUrl":"10.1016/j.cpb.2025.100495","url":null,"abstract":"<div><div><em>Polygonatum cyrtonema</em> Hua is traditional Chinese herbal medicine rich in active ingredients, such as polysaccharides, flavonoids, alkaloids, and others that contribute to human health. As a perennial plant, its culture duration may influence the accumulation of these functional active ingredients in its tissues. To investigate how secondary metabolites and gene expression levels change with plant growth age, we carried out metabolomic analysis on root tissues of 1-year-old and 2-year-old plants, alongside transcriptome analysis was of leaf tissues. Using LC-MS, we identified identified 81 and 80 differentially expressed metabolites（DEMs）in positive and negative ion modes, respectively. Of these, 80 differential metabolites showed higher accumulation in 2-year-old plants. The relative contents of nucleotides and analogues, lipids, organic acids, phenylpropanoids, alkaloids, and organic oxygen molecules increased with increasing growth age. Transcriptome analysis further revealed 2068 differentially expressed genes, including 1026 up-regulated and 1042 down-regulated genes. A total of 102 metabolic pathways were enriched, with glucose metabolism, phenylpropanoid biosynthesis and flavonoid biosynthesis, being among the most enriched metabolic pathways. These results show that longer culture duration enhances the active of metabolic pathways components. Overall, this study study integrates multi-omics techniques to demonstrate age-related changes in metabolites and genes profiles in <em>P. cyrtonema,</em> providing valuable insights for the identification of candidate genes involved in the biosynthesis of active substances.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100495"},"PeriodicalIF":5.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives of medicinal plants in modern healthcare setup: Phyto-bioinformatics, prevalent herbs for diseases, in-silico screening, machine learning involvement, Precise medicine","authors":"Arun Meyyazhagan","doi":"10.1016/j.cpb.2025.100494","DOIUrl":"10.1016/j.cpb.2025.100494","url":null,"abstract":"","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100494"},"PeriodicalIF":5.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphatidic acid accumulation in response to extended cold water imbibition disrupts membrane structure that inhibits germination of cotton (Gossypium hirsutum L.) seeds","authors":"Lakhvir Kaur Dhaliwal,&nbsp;Bandana Osti,&nbsp;Rosalyn B. Angeles-Shim","doi":"10.1016/j.cpb.2025.100491","DOIUrl":"10.1016/j.cpb.2025.100491","url":null,"abstract":"<div><div>This work aimed to understand glycerophospholipid regulation in cotton seeds that imbibed under cold stress and determine how such regulation affects overall germination performance. Lipidomics analysis showed that imbibition under cold stress drastically increase phosphatidic acid (PA) levels, while significantly reducing the content of other membrane lipids including phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine in seeds. The observed PA accumulation was associated with the upregulation of genes encoding phospholipases that catalyze PA synthesis through the hydrolysis of other membrane lipids. Alongside modifications in glycerophospholipid contents, the cold-imbibed seeds leaked excessively and germinated poorly, suggesting deleterious effects of cold-induced PA accumulation. Exogenous PA treatment of cotton seeds demonstrated a time- and concentration-dependent inhibition of germination, whereas suppression of phospholipase D activity under cold stress attenuated electrolyte leakage and improved seed germination. Together, these support our hypothesis on the harmful effects of excessive PA on seed germination. PA is conical and has a structural propensity to induce negative curvatures on membranes. Under cold stress, negative curvatures exacerbate cellular leakage which is injurious to germinating seeds. Microscopic analysis of PA-treated plant tissue indicates disruption in membrane structure that increased with increasing concentrations of PA. Based on our findings, we propose that under cold stress, germinating seeds rapidly synthesize PA via the phospholipase pathway to signal distress. With prolonged cold-water imbibition, however, induction of negative curvature due to PA accumulation combined with the breakdown of other membrane lipids disrupts membrane integrity, exacerbating cytoplasmic leakage and causing poor germination in seeds.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100491"},"PeriodicalIF":5.4,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance","authors":"Monica Marra ,&nbsp;Raul Pirona ,&nbsp;Laura Miozzi ,&nbsp;Daniele Marian ,&nbsp;Cinzia Montemurro ,&nbsp;Aldo Ceriotti ,&nbsp;Gian Paolo Accotto ,&nbsp;Elena Baldoni","doi":"10.1016/j.cpb.2025.100485","DOIUrl":"10.1016/j.cpb.2025.100485","url":null,"abstract":"<div><div>Durum wheat cultivation is increasingly threatened by viral diseases worldwide. Soil-borne cereal mosaic virus (SBCMV) and wheat spindle streak mosaic virus (WSSMV) cause significant crop losses in Europe. These viruses are transmitted through a soil-inhabiting vector, the plasmodiophoromycota <em>Polymyxa graminis</em> Led. There are very few methods available to eradicate <em>P. graminis</em>, whose resting spores survive in infested soil for decades, but they are either too expensive or not environmentally friendly. Therefore, it is crucial to develop resistant wheat varieties to mitigate the damage. For this purpose, more than 200 durum wheat genotypes, mostly landraces, were selected from the Global Durum Wheat Panel germplasm collection. Then, an experiment was conducted in a semi-controlled environment: the genotypes were sown in pots containing soil infested by <em>P. graminis</em> carrying SBCMV and WSSMV and maintained through the winter period. In early spring, visual assessment of viral symptomatology was performed. Subsequently, the viral loads of the two viruses in leaf tissues were determined through qRT-PCR analysis. The tested genotypes exhibited different responses to the two viruses: SBCMV showed very diversified viral loads among genotypes, whereas WSSMV infected all genotypes. We identified 23 genotypes, with low viral loads of both viruses and reduced symptoms, that could be of particular interest for breeders aiming at new resistant durum wheat varieties. A pilot GWAS allowed to identify genomic regions putatively associated to resistance to SBCMV or WSSMV, as well as possible candidate genes involved in these traits.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100485"},"PeriodicalIF":5.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of nanotechnology in agricultural sustainability: Absorption, translocation, and challenges of nanoparticles","authors":"Yanping Meng ,&nbsp;Yingying Feng ,&nbsp;Xin Bai ,&nbsp;Qinghui Yu ,&nbsp;Jiyang Zhou ,&nbsp;Juan Wang","doi":"10.1016/j.cpb.2025.100492","DOIUrl":"10.1016/j.cpb.2025.100492","url":null,"abstract":"<div><div>The global agricultural sector has seen significant expansion in recent years, which has raised concerns about food supply, the safety of fertilizers, and the use of genetically modified crops. The growing demand for food, coupled with various environmental issues, has highlighted the importance of adopting sustainable agricultural practices. Recent advancements in the field of nanotechnology suggest that it has the potential to greatly enhance agricultural sustainability, particularly in areas such as fertilizer application, genetic modification, and pest control. The effectiveness of nanomaterials in plants is heavily dependent on the plants' ability to absorb and translocate these nanoparticles. To maximize the efficiency of nanomaterials in the fields of agriculture and plant breeding, this article delves into several key aspects: the influence of the physical and chemical properties of nanoparticles on their translocation within the plant body, the impact of plant structural features on nanoparticle penetration and translocation, and the various ways in which nanoparticles are exposed within different plant species. Understanding these factors is crucial for researchers to gain a more comprehensive insight into how nanoparticles can overcome barriers within plant tissues and cells. It also sheds light on the primary factors that may restrict the effective delivery of nanoparticles. By addressing these challenges, the article aims to enhance the utilization efficiency of nanomaterials in the agricultural sector. This knowledge can facilitate the effective application of nanoparticles across various agricultural domains and foster the sustainable development of both nanotechnology and agriculture as a whole.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100492"},"PeriodicalIF":5.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of bioactive molecular networking to identify corneal-cytoprotective compounds from Dialium cochinchinense Pierre. leaves","authors":"Thi Tuyet Nhung Nguyen ,&nbsp;Yu-Chi Lin ,&nbsp;Le-Anh-Tuan Nguyen ,&nbsp;Chih-Hua Chao ,&nbsp;Thanh-Hoa Vo ,&nbsp;Su-Jung Hsu ,&nbsp;Keng-Chang Tsai ,&nbsp;Ching-Kuo Lee ,&nbsp;Jing-Jy Cheng","doi":"10.1016/j.cpb.2025.100489","DOIUrl":"10.1016/j.cpb.2025.100489","url":null,"abstract":"<div><div>Dry eye disease (DED) requires innovative therapeutic strategies targeting inflammation and oxidative stress. In this study, we demonstrate for the first time that <em>Dialium cochinchinense</em> leaf extracts exhibited significant anti-inflammatory activities by inhibiting the nuclear transcription factor-κB (NF-κB) signaling pathway in macrophages. Additionally, these extracts showed notable antioxidant and cytoprotective capabilities, effectively mitigating oxidative stress-induced damage in corneal epithelial cells. We employed an advanced bioactive molecular networking strategy to prioritize and isolate key bioactive compounds, providing distinct advantages over traditional phytochemical screening methods. This approach led to the discovery of a new compound, 6<em>S</em>,9<em>R</em>-2′-<em>O</em>-sinapoyl-roseoside, and 11 known phenolic compounds. Notably, 10 of these compounds exhibited substantial corneal-cytoprotective effects <em>in vitro</em>. Network pharmacology analysis revealed 65 key shared targets between these compounds and DED, linked to PI3K/AKT and MAPK pathways critical for inflammation and cell survival. Subsequent molecular docking and molecular dynamics simulations confirmed stable and high-affinity binding interactions between the identified lead compounds and key protein targets implicated in DED. These findings demonstrate <em>D. cochinchinense</em> leaf extract and its bioactive constituents are promising natural candidates for DED, providing a scientific basis for further translational research and potential drug development.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"42 ","pages":"Article 100489"},"PeriodicalIF":5.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}