The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L.

IF 5.4 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2025-01-31 DOI:10.1016/j.cpb.2025.100449

Nuo Wang, Yi Tu, Sirui Zeng, Jiabao Ye, Weiwei Zhang, Feng Xu, Yongling Liao

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引用次数: 0

Abstract

Terpene trilactones (TTLs) are important secondary metabolites in ginkgo tree (Ginkgo biloba), which play a crucial role in the treatment of human cardiovascular disease and prevention of thrombosis, and have been widely used in food, medicine and health products. However, there are few studies on the transcriptional modulation of TTLs biosynthesis and gene regulatory network of TTLs biosynthesis remains unclear. Here, we screened the transcription factor GbbZIP41 that may be involved in the biosynthesis of TTLs and verified its function by transgenic technology. The results showed that GbbZIP41 is a protein located in the nucleus and encodes 477 amino acids, which is highly expressed in G. biloba leaves. The analysis of total terpene content and GbbZIP41 gene expression in G. biloba leaves showed the opposite trend. In addition, the overexpression of GbbZIP41 gene in tobacco reduced the content of terpenoids and down-regulated the enzyme genes of MVA pathway and MEP pathway. Therefore, these results suggested that GbbZIP41 negatively regulates the biosynthesis of TTLs by inhibiting key enzyme genes in the TTLs synthesis pathway.

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.