Transcription factors participate in methyl jasmonate-induced diterpenoid biosynthesis in Andrographis paniculata

IF 4.5 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2025-08-05 DOI:10.1016/j.cpb.2025.100530

Yuan Li , Yue Shi , Yong Fan , Guangxi Ren , Dan Jiang , Kuangwei Cao , Yaogong Zhang , Zhengyan Li , Da Li , Chunsheng Liu

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引用次数: 0

Abstract

Andrographis paniculata is renowned for its wide range of pharmaceutical properties, largely owing to the presence of bioactive diterpenoids. However, the mechanism of methyl jasmonate (MeJA) -induced diterpenoid biosynthesis in A. paniculata remains poorly understood. In this study, we found that the MeJA-induced accumulation of diterpenoids was attributed to the increased expression of genes involved in diterpenoid biosynthetic pathways. Transient overexpression and Y1H assays revealed that ApMYC2, ApbZIP46, and ApWRKY33 were positive regulators that promoted the accumulation of diterpenoids by directly binding to the promoters of the downstream target gene ApUGT76E1. Thus, ApMYC2, ApbZIP46, and ApWRKY33 may be involved in the regulation of the diterpenoid biosynthesis pathway in A. paniculata. Overall, this research lays the groundwork for elucidating the molecular mechanism by which MYCs, bZIPs and WRKYs regulate the accumulation of diterpenoids in A. paniculata under MeJA induction. Our results provide a theoretical basis for the molecular breeding and quality improvement of A. paniculata in the future.

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转录因子参与茉莉酸甲酯诱导穿心莲二萜类生物合成

穿心莲以其广泛的药用特性而闻名，这主要是由于其生物活性二萜的存在。然而，茉莉酸甲酯（MeJA）诱导的甲草二萜类生物合成机制尚不清楚。在这项研究中，我们发现meja诱导的二萜积累归因于参与二萜生物合成途径的基因表达增加。瞬时过表达和Y1H实验显示，ApMYC2、ApbZIP46和ApWRKY33是正向调节因子，通过直接结合下游靶基因ApUGT76E1的启动子促进二萜的积累。因此，ApMYC2、ApbZIP46和ApWRKY33可能参与了金针桃二萜类生物合成途径的调控。综上所述，本研究为阐明MYCs、bZIPs和WRKYs在MeJA诱导下调控金穗二萜类物质积累的分子机制奠定了基础。本研究结果为今后金银花的分子育种和品质改良提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.