Plant Gene最新文献

{"title":"Molecular marker assisted gene stacking for multiple diseases resistance in an elite rice cultivar, BRRI dhan48","authors":"Mohammad Abdul Latif,&nbsp;Omar Kayess,&nbsp;Rakibul Hasan,&nbsp;Lutfur Rahman","doi":"10.1016/j.plgene.2025.100505","DOIUrl":"10.1016/j.plgene.2025.100505","url":null,"abstract":"<div><div>Blast and bacterial blight (BB) pose a significant threat to rice production. To enhance resistance to blast and BB, we employed marker-assisted backcross breeding to introgress the resistance genes into the genetic background of BRRI dhan48. The resistant (R) genes <em>Pi9</em>, <em>Pb1</em>, <em>Xa4</em>, <em>xa13</em>, and <em>Xa21</em> were introgressed from Pi9-US2, Pb1-US2, and IRBB58, respectively. The <em>Pb1</em> gene strengthens blast resistance in rice by interacting with WRKY45 transcription factor to modulate salicylic acid and phytoalexin pathways, while the <em>Pi9</em> gene triggers a signaling cascade involving reactive oxygen species (ROS) production and hormones like jasmonic acid and ethylene activation to enhance defense responses. <em>Xa4</em> strengthens the cell wall via WAK (cell wall-associated kinase) protein, hindering pathogen invasion, <em>xa13</em> disrupts SWEET (Sugar Will Eventually be Exported Transporter) protein function to limit sugar availability for <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> (Xoo). <em>Xa21</em> triggers mitogen-activated protein kinases (MAPKs)-mediated signaling cascades, leading to the activation of WRKY transcription factors that suppress bacterial proliferation. For that, triple cross was made, followed by repeated backcrosses, self-pollinations, and marker-assisted selection to generate BC₃F₅ progeny. Chi-square analysis of 380 BC₃F₂ individuals confirmed a monogenic inheritance pattern for blast and BB resistance traits. Finally, we selected 32 advanced lines, and among them nineteen lines possessed all these 5 R genes, i.e., <em>Pi9</em>, <em>Pb1</em>, <em>Xa4</em>, <em>xa13</em>, and <em>Xa21</em>_<em>,</em> while eight lines consisted of 4 R genes in different combinations. The disease rating of the advanced lines varied from 0 to 5 for both blast and BB diseases, while BRRI dhan48 had a disease rating ranging from 7 to 9. The lines G10, G25, G16, G31, G9, G2, and G20 demonstrated significantly higher grain yield (7.73, 7.72, 7.70, 7.65, 7.64, 7.59, and 7.58 t ha⁻¹, respectively) than the parent (7.00 t ha⁻¹). Investigation of marker trait association showed that molecular markers were negatively linked with blast and BB diseases. So, gene introgression by marker-assisted backcrossing (MABB) offers a robust and efficient way to identify and validate candidate genes with high precision for the development of durable, resistant rice breeding lines. These multiple disease resistant advanced lines may use as a potential genetic stock or incorporate with other desired genes for ensuring sustainable rice production under changing climatic conditions.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100505"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Genome-wide association and genomic prediction study of elite spring bread wheat (Triticum aestivum L.) genotypes under drought conditions across different locations” [Plant Gene Volume 39 (2024) 1–12/100461]","authors":"Lamyae Ed-Daoudy ,&nbsp;Zakaria El Gataa ,&nbsp;Laila Sbabou ,&nbsp;Wuletaw Tadesse","doi":"10.1016/j.plgene.2024.100467","DOIUrl":"10.1016/j.plgene.2024.100467","url":null,"abstract":"","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100467"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated metabolomic and transcriptomic insights into the role of anthocyanin and flavonoid biosynthesis in the pulp coloration mechanisms of Baccaurea ramiflora Lour. (Burmese grape)","authors":"Jianjian Huang ,&nbsp;Jie Chen ,&nbsp;Hui Zhu ,&nbsp;Yuzhong Zheng ,&nbsp;Xueying Wen ,&nbsp;Suying Cai ,&nbsp;Yingchun Zhu ,&nbsp;Mo Ding ,&nbsp;Qinghan Wu ,&nbsp;Zikai Chen ,&nbsp;Fengnian Wu","doi":"10.1016/j.plgene.2025.100510","DOIUrl":"10.1016/j.plgene.2025.100510","url":null,"abstract":"<div><div><em>Baccaurea ramiflora</em> Lour. (Burmese grape), a wild fruit tree with edible, ornamental, and medicinal qualities. The mechanism behind the color accumulation in its fruit pulp, which can be either pink or milky-white, remains unclear. This study investigates the metabolome and transcriptome of two <em>B. ramiflora</em> pulp types—LR (milky-white at maturity) and BR (pink at maturity)—to elucidate their coloration processes. We identified 35 flavonoids, including nine involved in the anthocyanin synthesis pathway, confirming cyanidin as the pivotal pigment for the pink pulp coloration. An examination of the flavonoid and anthocyanin biosynthetic pathways in <em>B. ramiflora</em> pulp uncovered 39 differentially expressed genes associated with structural genes. The genes <em>F3′5′H</em> and <em>UFGT</em> exhibited high expression levels in the first two developmental stages of BR, significantly more than in LR, and were almost non-existent in later stages, signifying their crucial role in the differential color accumulation between BR and LR pulps. Additionally, the expression levels of <em>CHI</em> and <em>FLS</em>, early-stage structural genes in the anthocyanin synthesis pathway, correlated with the concentrations of naringenin and quercetin, indicating their importance in the anthocyanin biosynthesis pathway of <em>B. ramiflora</em> pulp. These discoveries provide new insights that could facilitate the breeding of <em>B. ramiflora</em> varieties with diverse pulp colors.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100510"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and comparative analysis of flowering genes in the Sugar pathway from five Gossypium species","authors":"Lei-Ming Liu ,&nbsp;Chuan-Bo Jiang ,&nbsp;Yi-Lin Yang ,&nbsp;Tian-Run Mei ,&nbsp;Ruo-Fei Liu ,&nbsp;Hai-Liang Liu ,&nbsp;Xian-Zhong Huang","doi":"10.1016/j.plgene.2025.100500","DOIUrl":"10.1016/j.plgene.2025.100500","url":null,"abstract":"<div><div>Flowering is a critical plant growth stage coordinated by internal and external factors. Changes to endogenous sugar levels can promote or inhibit flowering, but research on the regulation of flowering-related genes associated with the sugar pathway in cotton is limited. Here, a genome-wide study identified 165 flowering and sugar pathway-related genes in five cotton species: <em>Gossypium herbaceum</em>, <em>G. arboreum</em>, <em>G. hirsutum</em>, <em>G. barbadense</em>, and <em>G. raimondii</em>. The genes were phylogenetically classified into nine subfamilies and showed a high degree of conservation. Notably, no homologs of <em>INDETERMINATE DOMAIN 8</em> (<em>IDD8</em>), <em>SUCROSE-PROTON SYMPORTER 9</em> (<em>SUC9</em>), or <em>AGP GALACTOSYLTRANSFERASE 2</em> (<em>GALT2</em>) were identified. A synteny analysis provided evidence of varying degrees of gene expansion, and a selection pressure analysis indicated that the genes had undergone purifying selection, with <em>Ka</em>/<em>Ks</em> ratios of &lt;1. The similarity among the genes identified in <em>G. herbaceum</em>, <em>G. arboreum</em>, and <em>G. raimondii</em> was higher than between these species and the allopolyploid cotton species, indicating the earlier divergence of these genes. A network analysis of protein interaction revealed <em>G. hirsutum</em> proteins to be associated primarily with sugar synthesis, transport, and metabolism. Yeast two-hybrid assays demonstrated that GhTPS1–1 and GhHXK1–1 can interact with GhPGI1–1. RNA-sequencing data for 46 genes from eight tissue-types in <em>G. hirsutum</em> revealed that most were highly expressed in stems and flowers. This study provides a comprehensive phylogenetic and network analysis of flowering-related genes in the sugar pathway across five <em>Gossypium</em> species, laying a foundation for future in-depth research on the functional mechanisms of these genes.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100500"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel genetic control of alpha-amylase activity during pre-harvest sprouting indicated by RNA sequencing of soft winter wheat varieties Cardinal and Logan","authors":"Bryan W. Penning","doi":"10.1016/j.plgene.2025.100495","DOIUrl":"10.1016/j.plgene.2025.100495","url":null,"abstract":"<div><div>Pre-harvest sprouting, the germination of wheat seeds still in the field, leads to decreased crop value and increased crop losses around the world. Pre-harvest sprouting causes low Falling Number that results in price reductions or rejection of soft winter wheat grain at the flour mill or grain elevator. Several pre-harvest sprouting resistance genes have been found. However, they act through seed dormancy-related signaling pathways which can affect winter wheat root establishment for overwintering and double-cropping wheat with soybean. Here, RNA sequencing has been performed on soft red winter wheat varieties Cardinal and Logan with 91 % of 1978 markers common between them but differing in pre-harvest sprouting resistance. Between 64 and 660 genes were differentially expressed in comparisons between Cardinal and Logan at different time points of seed development. A <em>glucan water diskinase</em> was a top five differentially expressed gene located near a recently reported quantitative trait locus for pre-harvest sprouting. It has potential to reduce alpha amylase activity and starch degradation during pre-harvest sprouting. Slowing starch degradation reduces starch damage, keeping flour pasting viscosity and Falling Number higher under pre-harvest sprouting conditions. Slowing starch damage rather than altering seed dormancy would reduce impact on some agronomic traits.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100495"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study of tea plant based on SLAF-seq revealed SNP variations regulating timing of bud flush","authors":"Xiaozeng Mi ,&nbsp;Dahe Qiao ,&nbsp;Yanlin An ,&nbsp;Chun Yang ,&nbsp;Kaiqin Lin ,&nbsp;Congling Peng ,&nbsp;Juan Chen","doi":"10.1016/j.plgene.2025.100511","DOIUrl":"10.1016/j.plgene.2025.100511","url":null,"abstract":"<div><div>As one of the most important agronomic traits of the tea plant (<em>Camellia sinensis</em>), the timing of bud flush (TBF) affects the sales price of tea beverages. Here, we performed specific-locus amplified fragment (SLAF) sequencing and genome-wide association study (GWAS) analysis on 200 offspring of natural hybridization from the ‘Qianmei 601’. A total of 796,294 SLAF tags and 2,324,211 single-nucleotide polymorphisms (SNPs) were obtained by sequencing. The 200 tea germplasms were clustered into four groups by population structure, principal component analysis (PCA) and phylogenetic tree analysis. GWAS identified twelve SNPs loci that were significantly associated with TBF traits. Seven candidate genes associated with TBF were subsequently identified by scanning in the regions within 1 Mb SNP loci. In addition, we found that two candidate genes (<em>ARP</em> and <em>WRKY</em>) were highly expressed in individuals with early TBF. Interestingly, cloning and sequencing of the <em>ARP</em> gene revealed C-G variants between the early and late TBF individuals. Our results provide a theoretical basis for early TBF breeding and functional gene analysis in tea plants.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100511"},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary dynamics of Senna: Perspectives from plastome evolution and phylogenomics","authors":"Samaila Samaila Yaradua ,&nbsp;Faten Zubair Filimban","doi":"10.1016/j.plgene.2024.100486","DOIUrl":"10.1016/j.plgene.2024.100486","url":null,"abstract":"<div><div>Genus <em>Senna</em> (Caesalpinioideae, <em>Fabaceae</em>) comprises about 350 species mainly distributed in the American continent. Despite the sequencing and assembly of the chloroplast genome of some of the species in the genus, the chloroplast genome sequence and structural variation have not been evaluated, and the evolutionary relationship within the genus is still lacking. In this study, we sampled different species of the genus representing the six sections to examine the variation in the genome and phylogenetic relationships. The chloroplast genome of <em>Senna</em> has a typical quadripartite structure with a size ranging from 148, 437 bp to 162, 426 bp. There are gene losses in some species of the genus, the earliest diverged species lost 9 protein-coding genes which were recovered in the latter diverged lineages. No inversion was detected in the genome but there is a significant level of sequence variation, gene number, and genome size, that provide valuable information on the evolution of the genus. The following regions are the most variable regions in the genus and can be used as DNA barcodes for the identification of the species: <em>Psba, ndhH, trnT-trnL</em>, <em>trnG-trnR</em>, <em>rps18</em>-<em>rpl20</em>, and <em>trnI-rrn16</em>. The phylogenomic analysis revealed a well-resolved phylogenetic relationship of the genus, supporting the monophyly of the genus. The phylogenetic tree reported the phylogenetic positions and sister relationships of some species for the first time. Our result revealed that section Chameafistula is paraphyletic and calls for the revision of the infrageneric classification of the genus.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"41 ","pages":"Article 100486"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saffron corms transcriptome analysis reveals genes associated with corm rot disease","authors":"Ali Darvishian ,&nbsp;Farhad Nazarian-Firouzabadi ,&nbsp;Ahmad Ismaili ,&nbsp;Mostafa Darvishnia","doi":"10.1016/j.plgene.2024.100488","DOIUrl":"10.1016/j.plgene.2024.100488","url":null,"abstract":"<div><div>Saffron (<em>Crocus sativus</em> L.) is by far the most expensive spice in the world. Low water requirements, straightforward agricultural practices, diverse applications in the food, pharmaceutical and textile industries, are major advantages for its widely cultivation worldwide. Saffron is genetically sterile and hence it is propagated through corms. As a perennial plant, its corms remain in the field for at least three consecutive cultivation seasons, increasing their susceptibility to soilborne pathogens. Among devastating soilborne plant pathogens, <em>Fusarium oxysporum</em> f. sp., the causal agent of corm rot, poses a significant threat to saffron cultivation globally. The development of effective management strategies against <em>F. oxysporum</em> is impeded by the absence of resistant genotypes and a limited understanding of its pathogenicity. To assess and find genes associated with the response to <em>F. oxysporum</em> infection, the corm transcriptome was analyzed following <em>F. oxysporum</em> inoculation by RNA-seq. More than 294,520 and 243,328 transcripts were documented. Out of 168,448 identified unigenes, 34,862, 15,234 and 27,213 unigenes were annotated in NCBI non-redundant (Nr) protein database, GO and UniProtKB/Swiss-Prot databases, respectively. Out of 38,643 annotated genes, 2169 genes exhibited differential expression in response to <em>F. oxysporum.</em> Among these, 1832 genes showed decreased expression, whereases 337 genes displayed increased expression. Quantitative RT-PCR analysis confirmed the expression patterns of 10 hub genes, including chitinase, a serine/threonine kinase and a WRKY transcription factor gene. In contrast, the relative expression of a pectin methylesterase, a Flavodoxin-like quinone oxidoreductase and HSP genes were found to be down-regulated. The findings of this study clearly demonstrate that <em>F. oxysporum</em> infection significantly perturbs the expression of corms R-genes, suggesting that such genes can be used to improve saffron plant resistance to fungal and oomycetes soilborne pathogens in future breeding programs.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"41 ","pages":"Article 100488"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143177774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the complete CDS sequence of Bibenzyl synthase (BBS) gene in Pholidota articulata and its transcriptional regulation under different stress conditions","authors":"Swagata Debnath,&nbsp;Suman Kumaria","doi":"10.1016/j.plgene.2025.100491","DOIUrl":"10.1016/j.plgene.2025.100491","url":null,"abstract":"<div><div>Bibenzyl synthase (BBS) is one of the key enzymes for the formation of bibenzyls, a class of specialized therapeutic metabolites that are predominantly found in the family Orchidaceae. The present study reports the complete transcript of the <em>BBS</em> gene isolated and sequenced from an ethnomedicinally important orchid, <em>Pholidota articulata</em> Lindl. The <em>BBS</em> transcript of <em>P. articulata</em> (<em>PaBBS</em>) was found to comprise a total of 1494 bp with an open reading frame coding for 390 amino acids. Multiple sequence analysis and phylogenetic analysis highlighted its close association with <em>BBS</em> sequences and type III polyketide synthase (PKS III) genes of other plant species. Three-dimensional structure analysis has revealed that the PaBBS protein is a homodimer with each subunit having a molecular weight of 42.83 KDa. <em>In silico</em> studies of the putative PaBBS protein revealed the presence of the characteristic conserved regions of the BBS enzyme as well as the PKS III superfamily. The present study also revealed that expression <em>PaBBS</em> is influenced by the age of the plant, tissue type, abiotic stress, and exposure to elicitors. The expression of <em>PaBBS</em> transcript was recorded to be higher in young plantlets as compared to mature plants, and among the plant parts the root tissue exhibited the highest expression. Further, it was found that the expression of <em>PaBBS</em> was upregulated by abiotic stresses such as wounding and heat treatment, downregulated by high-intensity UV exposure and cold treatment, and increased many-fold when exposed to elicitors like chitosan and yeast extract. The results obtained in the present study provide an understanding of the transcriptional regulation of <em>PaBBS</em> gene which plays a crucial role in the ecological adaptation of <em>P. articulata</em>, an orchid of medicinal importance<em>.</em></div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"41 ","pages":"Article 100491"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic patterns of understudied species within the plant genus Brassica: Future prospects in the post genomics-era","authors":"Parthiban Subramanian ,&nbsp;Hemavathi Brijesh ,&nbsp;Bum-Soo Hahn","doi":"10.1016/j.plgene.2024.100489","DOIUrl":"10.1016/j.plgene.2024.100489","url":null,"abstract":"<div><div><em>Brassica</em> remains to be one of the agriculturally important genera of plants worldwide due to its diverse nature of crops cultivated all across the globe. Despite the rich species diversity within the genus <em>Brassica</em>, detailed studies on phylogeny and evolutionary origins have primarily focused on just six species, which have given rise to nearly all of the currently cultivated Brassica crops. Continuous breeding practices for specific traits in these six species have led to significant challenges, including nutrient depletion, increased susceptibility to plant pathogens, and vulnerability to changing climate conditions in the present generation of crops. Lack of established phylogenetic relationships among the species of <em>Brassica</em> remains to be a significant barrier hindering error-free conservation and use of <em>Brassica</em> resources. We have identified putative phylogenetic relationships between <em>Brassica</em> crop wild relatives (CWRs) by reinforcing data from several genetic markers and metabolite based studies. Supplementation of genomic data for a robust phylogeny and current status of availability of genomic data of <em>Brassica</em> crop wild relatives (CWRs) from public database has been highlighted. This report identifies critical gaps in <em>Brassica</em> research addressing of which would facilitate effective conservation and use of <em>Brassica</em> genetic resources.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"41 ","pages":"Article 100489"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}