Apoptotic chromatin condensation inducer in the nucleus: Genome-wide analysis in plants and expression profile during Cowpea Severe Mosaic Virus infection in Vigna unguiculata [L.] Walp

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2024-06-13 DOI:10.1016/j.plgene.2024.100459

Felipe Castro Teixeira , Erica Monik Silva Roque , Alex Martins Aguiar , Sâmia Alves Silva , Victor Breno Faustino Bezerra , Otávio Hugo Aguiar Gomes , Luciano Gomes Fietto , Murilo Siqueira Alves

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引用次数: 0

Abstract

Apoptotic Chromatin Condensation Inducer in the Nucleus (ACIN1) is a scaffold protein that was first described as a complex component responsible for triggering apoptosis in human cells. In plants, ACIN1 participates in silencing of Flowering Locus C (FLC), involved in vernalization in Arabidopsis thaliana. Contrary to what has been observed for humans, there are no reports on ACIN1 linked to programmed cell death (PCD) in plants. Actually, the function of ACIN1 in plants is still poorly understood. In the present study, a genome-wide analysis of the ACIN1 gene family in plants identified 27 ACIN1 orthologs from 19 species belonging to 12 plant families. The phylogenetic relationships, physicochemical properties, gene structure, conserved motifs, promoter cis-elements, chromosomal localization, syntenic regions, and protein network were investigated. Altogether, these analyzes revealed highly conserved domains in the structure of the ACIN1 proteins, as well as putative metacaspase cleavage sites, which suggest that they play a conserved function probably associated with the programmed cell death in plants. For instance, differential expression pattern and modulation of ACIN1 were noticed after inoculation of cowpea with Cowpea severe mosaic virus (CPSMV). Therefore, this study was conducted to provide, for the first time, information on the evolutionary, structural, and functional characteristics of the ACIN1 gene family as an initial effort towards understanding the role of these proteins in studied plant development and stress responses.

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细胞核中凋亡染色质凝聚诱导剂：植物的全基因组分析以及豇豆严重花叶病毒感染期间的表达概况

细胞核中的凋亡染色质凝集诱导因子（ACIN1）是一种支架蛋白，最早被描述为引发人类细胞凋亡的复合成分。在植物中，ACIN1 参与拟南芥春化过程中开花基因座 C（FLC）的沉默。与在人类中观察到的情况相反，目前还没有关于 ACIN1 与植物中的程序性细胞死亡（PCD）有关的报道。实际上，人们对 ACIN1 在植物中的功能仍然知之甚少。本研究对植物中的 ACIN1 基因家族进行了全基因组分析，发现了来自 12 个植物科 19 个物种的 27 个 ACIN1 同源物。研究人员对其系统发育关系、理化性质、基因结构、保守基序、启动子顺式元件、染色体定位、同源区和蛋白质网络进行了调查。总之，这些分析揭示了 ACIN1 蛋白结构中高度保守的结构域，以及假定的元天冬酶裂解位点，这表明它们发挥着保守的功能，可能与植物细胞的程序性死亡有关。例如，在豇豆接种豇豆严重花叶病毒（CPSMV）后，ACIN1 的表达模式和调控均有所不同。因此，本研究首次提供了有关 ACIN1 基因家族的进化、结构和功能特征的信息，为了解这些蛋白在研究植物发育和胁迫响应中的作用做出了初步努力。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.