Current protocols in mouse biology最新文献_第9页

Cardiovascular Imaging in Mice 小鼠心血管影像学研究

Current protocols in mouse biology Pub Date : 2016-03-01 DOI: 10.1002/9780470942390.mo150122

Colin K. L. Phoon, D. Turnbull

引用次数: 21

Analysis of Mitochondrial Respiratory Chain Supercomplexes Using Blue Native Polyacrylamide Gel Electrophoresis (BN‐PAGE) 蓝色天然聚丙烯酰胺凝胶电泳(BN‐PAGE)分析线粒体呼吸链超配合物

Current protocols in mouse biology Pub Date : 2016-03-01 DOI: 10.1002/9780470942390.mo150182

Pooja Jha, Xu Wang, J. Auwerx

引用次数: 176

Generating Mouse Models Using CRISPR‐Cas9‐Mediated Genome Editing 使用CRISPR - Cas9介导的基因组编辑生成小鼠模型

Current protocols in mouse biology Pub Date : 2016-03-01 DOI: 10.1002/9780470942390.mo150178

Wenning Qin, Peter M. Kutny, R. Maser, S. Dion, Jeffrey D. Lamont, Yingfan Zhang, Greggory A. Perry, Haoyi Wang

引用次数: 42

Voluntary Wheel Running in Mice 小鼠自愿滚轮跑

Current protocols in mouse biology Pub Date : 2015-12-02 DOI: 10.1002/9780470942390.mo140295

Jorming Goh, Warren Ladiges

引用次数: 84

Optimized Heart Sampling and Systematic Evaluation of Cardiac Therapies in Mouse Models of Ischemic Injury: Assessment of Cardiac Remodeling and Semi-Automated Quantification of Myocardial Infarct Size 优化心脏采样和缺血性损伤小鼠心脏治疗的系统评估:心脏重塑的评估和心肌梗死面积的半自动化量化

Current protocols in mouse biology Pub Date : 2015-12-02 DOI: 10.1002/9780470942390.mo140293

Mariana Valente, Ana Araújo, Tiago Esteves, Tiago L. Laundos, Ana G. Freire, Pedro Quelhas, Perpétua Pinto-do-Ó, Diana S. Nascimento

{"title":"Optimized Heart Sampling and Systematic Evaluation of Cardiac Therapies in Mouse Models of Ischemic Injury: Assessment of Cardiac Remodeling and Semi-Automated Quantification of Myocardial Infarct Size","authors":"Mariana Valente, Ana Araújo, Tiago Esteves, Tiago L. Laundos, Ana G. Freire, Pedro Quelhas, Perpétua Pinto-do-Ó, Diana S. Nascimento","doi":"10.1002/9780470942390.mo140293","DOIUrl":"10.1002/9780470942390.mo140293","url":null,"abstract":"Cardiac therapies are commonly tested preclinically in small-animal models of myocardial infarction. Following functional evaluation, post-mortem histological analysis is essential to assess morphological and molecular alterations underlying the effectiveness of treatment. However, non-methodical and inadequate sampling of the left ventricle often leads to misinterpretations and variability, making direct study comparisons unreliable. Protocols are provided for representative sampling of the ischemic mouse heart followed by morphometric analysis of the left ventricle. Extending the use of this sampling to other types of in situ analysis is also illustrated through the assessment of neovascularization and cellular engraftment in a cell-based therapy setting. This is of interest to the general cardiovascular research community as it details methods for standardization and simplification of histo-morphometric evaluation of emergent heart therapies. © 2015 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"5 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470942390.mo140293","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50687226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Mouse Primary Hepatocyte Culture Model for Studies of Circadian Oscillation 小鼠原代肝细胞培养模型的昼夜振荡研究

Current protocols in mouse biology Pub Date : 2015-12-02 DOI: 10.1002/9780470942390.mo150101

Penny C. Molyneux, Lorna A. Pyle, Martha Dillon, Mary E. Harrington

{"title":"A Mouse Primary Hepatocyte Culture Model for Studies of Circadian Oscillation","authors":"Penny C. Molyneux, Lorna A. Pyle, Martha Dillon, Mary E. Harrington","doi":"10.1002/9780470942390.mo150101","DOIUrl":"10.1002/9780470942390.mo150101","url":null,"abstract":"Circadian rhythms regulate many aspects of behavior and physiological processes, and, through external signals, help an organism entrain to its environment. These rhythms are driven by circadian clocks in many cells and tissues within our bodies, and are synchronized by a central pacemaker in the brain, the suprachiasmatic nucleus. Peripheral oscillators include the liver, whose circadian clock controls persistent daily rhythms in gene expression and in liver-specific functions such as metabolic homeostasis and drug metabolism. Chronic circadian clock disruption, as in rotating shiftwork, has been linked to disorders including obesity, diabetes, and cardiovascular disease. The mouse primary hepatocyte culture model allows the examination of circadian rhythms in these cells. This article describes a transgenic mouse model that uses a bioluminescent reporter to examine the circadian properties of a core clock gene Period2. Hepatocytes are isolated using a modified collagenase perfusion technique and cultured in a sandwich configuration, then sealed in a buffered medium containing luciferin for detection of whole-culture or single-cell bioluminescence. After synchronization by a medium change, cultures demonstrate coherent circadian period and phase measures of bioluminescence from the PERIOD2::LUCIFERASE reporter. © 2015 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"5 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470942390.mo150101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50687369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Assessing Cognition in Mice 评估小鼠的认知能力

Current protocols in mouse biology Pub Date : 2015-12-02 DOI: 10.1002/9780470942390.mo150068

Sabine M. Hölter, Lillian Garrett, Jan Einicke, Bettina Sperling, Petra Dirscherl, Annemarie Zimprich, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabě de Angelis, Wolfgang Wurst

{"title":"Assessing Cognition in Mice","authors":"Sabine M. Hölter, Lillian Garrett, Jan Einicke, Bettina Sperling, Petra Dirscherl, Annemarie Zimprich, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabě de Angelis, Wolfgang Wurst","doi":"10.1002/9780470942390.mo150068","DOIUrl":"10.1002/9780470942390.mo150068","url":null,"abstract":"Genetically modified mouse models have proven useful to study learning and memory processes and the neurocircuitry and molecular mechanisms involved, as well as to develop therapies for diseases involving cognitive impairment. A variety of tests have been developed to measure cognition in mice, and here we present those established and regularly used in the German Mouse Clinic. The test paradigms have been carefully chosen according to reliability of results and disease relevance of the cognitive functions assessed. Further criteria were time efficiency and ease of application. All tests assess slightly different but also overlapping or interacting aspects of learning and memory so that they can be used to complement each other in a comprehensive assessment of cognitive function. The five protocols described are for spontaneous alternation in the Y-maze, social discrimination, object recognition, automated assessment of learning and memory using the IntelliCage, and olfactory discrimination learning. © 2015 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"5 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470942390.mo150068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50687719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Tests for Anxiety-Related Behavior in Mice 小鼠焦虑相关行为的测试

Current protocols in mouse biology Pub Date : 2015-12-02 DOI: 10.1002/9780470942390.mo150010

Sabine M. Hölter, Jan Einicke, Bettina Sperling, Annemarie Zimprich, Lillian Garrett, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabé de Angelis, Wolfgang Wurst

引用次数: 38

Mouse Necropsy. 老鼠尸体剖检。

Current protocols in mouse biology Pub Date : 2015-09-01 DOI: 10.1002/9780470942390.mo140296

Piper M Treuting, Jessica M Snyder

引用次数: 17

An Overview of Typical Infections of Research Mice: Health Monitoring and Prevention of Infection. 研究小鼠典型感染综述:健康监测与感染预防。

Current protocols in mouse biology Pub Date : 2015-09-01 DOI: 10.1002/9780470942390.mo150023

James R Fahey, Haiyan Olekszak

{"title":"An Overview of Typical Infections of Research Mice: Health Monitoring and Prevention of Infection.","authors":"James R Fahey, Haiyan Olekszak","doi":"10.1002/9780470942390.mo150023","DOIUrl":"https://doi.org/10.1002/9780470942390.mo150023","url":null,"abstract":"There are many reasons to keep research mice healthy and free from infections. The two most important of these are to protect the health and welfare of research mice and to prevent infections from negatively impacting research. Just as the genetic integrity of a mouse strain will influence the reproducibility and validity of research data, so too will the microbiologic integrity of the animals. This has been repeatedly demonstrated in the literature of laboratory animal sciences wherein the direct impact of infections on physiologic parameters under study have been described. Therefore, it is of great importance that scientists pay close attention to the health status of their research animal colonies and maintain good communications with the animal facility personnel at their institution about mouse health issues. This overview provides information about animal health monitoring (HM) in research mouse colonies including commonly monitored agents, diagnostic methods, HM program, risk assessment, and animal facility biosecurity. Lastly, matters of communication with laboratory animal professionals at research institutions are also addressed.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"5 3","pages":"235-245"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162237/pdf/CPMO-5-235.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34038349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3