评估小鼠的认知能力

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2015-12-02 DOI:10.1002/9780470942390.mo150068

Sabine M. Hölter, Lillian Garrett, Jan Einicke, Bettina Sperling, Petra Dirscherl, Annemarie Zimprich, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabě de Angelis, Wolfgang Wurst

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引用次数: 56

摘要

转基因小鼠模型已被证明对研究学习和记忆过程以及所涉及的神经回路和分子机制，以及开发涉及认知障碍的疾病的治疗方法有用。已经开发了各种各样的测试来测量小鼠的认知能力，在这里我们介绍那些在德国小鼠诊所建立并经常使用的测试。根据评估结果的可靠性和认知功能的疾病相关性，仔细选择了测试范式。进一步的标准是时间、效率和应用的便利性。所有测试评估的学习和记忆方面略有不同，但也有重叠或相互作用，因此它们可以在全面评估认知功能时相互补充。所描述的五种协议分别是y迷宫的自发交替、社会歧视、对象识别、使用IntelliCage的学习和记忆自动评估以及嗅觉歧视学习。©2015 by John Wiley &儿子,Inc。

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Assessing Cognition in Mice

Genetically modified mouse models have proven useful to study learning and memory processes and the neurocircuitry and molecular mechanisms involved, as well as to develop therapies for diseases involving cognitive impairment. A variety of tests have been developed to measure cognition in mice, and here we present those established and regularly used in the German Mouse Clinic. The test paradigms have been carefully chosen according to reliability of results and disease relevance of the cognitive functions assessed. Further criteria were time efficiency and ease of application. All tests assess slightly different but also overlapping or interacting aspects of learning and memory so that they can be used to complement each other in a comprehensive assessment of cognitive function. The five protocols described are for spontaneous alternation in the Y-maze, social discrimination, object recognition, automated assessment of learning and memory using the IntelliCage, and olfactory discrimination learning. © 2015 by John Wiley & Sons, Inc.

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.