小鼠自愿滚轮跑

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2015-12-02 DOI:10.1002/9780470942390.mo140295

Jorming Goh, Warren Ladiges

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引用次数: 84

摘要

在老鼠身上进行自愿滚轮跑步被用来评估身体表现和耐力，并模拟运动训练作为一种增强健康的方式。轮跑是一种自愿的活动，与其他小鼠实验运动模型相反，后者依赖于厌恶刺激来强迫主动运动。这个实验方案包括让老鼠在一个放置在标准老鼠笼子里的倾斜的塑料碟形轮子的开放表面上自由奔跑。旋转以电子方式传输到USB集线器，这样就可以通过软件程序捕获运行频率和速度，用于数据存储和分析可变时间段。老鼠被单独饲养，以便对每只动物进行准确的记录。小鼠品系、性别、年龄和个体动机等影响跑步活动的因素，必须在设计自愿轮跑实验时加以考虑。©2015 by John Wiley &儿子,Inc。

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Voluntary Wheel Running in Mice

Voluntary wheel running in the mouse is used to assess physical performance and endurance and to model exercise training as a way to enhance health. Wheel running is a voluntary activity in contrast to other experimental exercise models in mice, which rely on aversive stimuli to force active movement. This protocol consists of allowing mice to run freely on the open surface of a slanted, plastic saucer-shaped wheel placed inside a standard mouse cage. Rotations are electronically transmitted to a USB hub so that frequency and rate of running can be captured via a software program for data storage and analysis for variable time periods. Mice are individually housed so that accurate recordings can be made for each animal. Factors such as mouse strain, gender, age, and individual motivation, which affect running activity, must be considered in the design of experiments using voluntary wheel running. © 2015 by John Wiley & Sons, Inc.

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.