Tests for Anxiety-Related Behavior in Mice

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2015-12-02 DOI:10.1002/9780470942390.mo150010

Sabine M. Hölter, Jan Einicke, Bettina Sperling, Annemarie Zimprich, Lillian Garrett, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabé de Angelis, Wolfgang Wurst

引用次数: 38

Abstract

Phenotyping of inbred mouse strains and genetically modified mouse models for characteristics related to neuropsychiatric diseases includes assessing their anxiety-related behavior. A variety of tests have been developed to measure anxiety in laboratory rodents and these tests have been placed under scrutiny over the years concerning their validity. Here we describe the most widely used tests for anxiety in mice. The protocols we present are established methods used in the German Mouse Clinic (GMC), with which alterations in anxiety could successfully be discovered in mouse mutants. Moreover, since baseline anxiety levels in mice are easily influenced by a great variety of disturbances, we carefully outline the critical parameters that need to be considered. © 2015 by John Wiley & Sons, Inc.

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小鼠焦虑相关行为的测试

近交小鼠品系和转基因小鼠模型与神经精神疾病相关特征的表型分析包括评估其焦虑相关行为。人们开发了各种各样的测试来测量实验室啮齿动物的焦虑，这些测试多年来一直在审查它们的有效性。在这里，我们描述了最广泛使用的老鼠焦虑测试。我们提出的方案是在德国小鼠诊所(GMC)中使用的既定方法，可以成功地在小鼠突变体中发现焦虑的改变。此外，由于小鼠的基线焦虑水平很容易受到各种干扰的影响，我们仔细地概述了需要考虑的关键参数。©2015 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.