优化心脏采样和缺血性损伤小鼠心脏治疗的系统评估:心脏重塑的评估和心肌梗死面积的半自动化量化

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2015-12-02 DOI:10.1002/9780470942390.mo140293

Mariana Valente, Ana Araújo, Tiago Esteves, Tiago L. Laundos, Ana G. Freire, Pedro Quelhas, Perpétua Pinto-do-Ó, Diana S. Nascimento

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引用次数: 3

摘要

心脏治疗通常在小动物心肌梗死模型中进行临床前测试。在功能评估之后，死后组织学分析对于评估治疗有效性的形态学和分子改变至关重要。然而，非系统的和不充分的左心室采样经常导致误解和变异性，使直接研究比较不可靠。提供了有代表性的小鼠缺血心脏采样，然后进行左心室形态计量学分析的方案。通过在细胞为基础的治疗环境中对新生血管和细胞植入的评估，也说明了将这种采样的使用扩展到其他类型的原位分析。这是一般心血管研究界感兴趣的，因为它详细说明了紧急心脏治疗的组织形态计量学评估的标准化和简化方法。©2015 by John Wiley &儿子,Inc。

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Optimized Heart Sampling and Systematic Evaluation of Cardiac Therapies in Mouse Models of Ischemic Injury: Assessment of Cardiac Remodeling and Semi-Automated Quantification of Myocardial Infarct Size

Cardiac therapies are commonly tested preclinically in small-animal models of myocardial infarction. Following functional evaluation, post-mortem histological analysis is essential to assess morphological and molecular alterations underlying the effectiveness of treatment. However, non-methodical and inadequate sampling of the left ventricle often leads to misinterpretations and variability, making direct study comparisons unreliable. Protocols are provided for representative sampling of the ischemic mouse heart followed by morphometric analysis of the left ventricle. Extending the use of this sampling to other types of in situ analysis is also illustrated through the assessment of neovascularization and cellular engraftment in a cell-based therapy setting. This is of interest to the general cardiovascular research community as it details methods for standardization and simplification of histo-morphometric evaluation of emergent heart therapies. © 2015 by John Wiley & Sons, Inc.

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.