Veterinary and Animal Science最新文献

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Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-∆VirB12 区分感染动物与接种动物:NH-AGID测定在接种流产布鲁氏菌A19-∆VirB12的母牛中的验证和应用
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-26 DOI: 10.1016/j.vas.2025.100530
Feng Ye , Xiao-jing Ma , Li-ya Liu , Wen-xi Gu , Wen-liang Yan , Xu Zhang , Jia-rui Zhang , Xin-ping Yi
{"title":"Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-∆VirB12","authors":"Feng Ye ,&nbsp;Xiao-jing Ma ,&nbsp;Li-ya Liu ,&nbsp;Wen-xi Gu ,&nbsp;Wen-liang Yan ,&nbsp;Xu Zhang ,&nbsp;Jia-rui Zhang ,&nbsp;Xin-ping Yi","doi":"10.1016/j.vas.2025.100530","DOIUrl":"10.1016/j.vas.2025.100530","url":null,"abstract":"<div><div>Differentiating infected from vaccinated animals is crucial for clinical diagnosis in brucellosis control. The objective of this study was to assess the performance of native hapten agar gel immuno-diffusion(NH-AGID) in differential identification of infected heifers from vaccined with strain A19-∆VirB12 and identifying shedding animals at the serologic level. 30 heifers were vaccined with A19-∆VirB12, and corresponding <strong>s</strong>erum and vaginal swab samples were collected at predetermined intervals. Serological assessments were conducted using the Rose-bengal test, serum agglutination test, competitive enzyme-linked immunosorbent assay and NH-AGID. <em>Brucella</em>-positive sera were further distinguished using differential identification ELISA and NH-AGID. Vaginal swab DNA was tested using AMOS-PCR and dual real-time fluorescence PCR. Results showed that NH-AGID demonstrated a high specificity and positive predictive values (PPV) for diagnosis of brucellosis. Additionally, the diagnostic accuracy of NH-AGID for infected and shedding animals was more than 90 %. Heifers vaccinated with A19-∆VirB12 were serologically confirmed as infected by NH-AGID and also tested positive by PCR. The application of NH-AGID effectively identified infected and shedding animals from vaccination with A19-∆VirB12, thereby supporting the brucellosis decontamination efforts.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100530"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of European authorised level of zinc from different sources on the physiology and intestinal ecosystem and performance of piglets weaned at different ages 不同来源锌对不同日龄断奶仔猪生理、肠道生态系统及生产性能的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-10 DOI: 10.1016/j.vas.2025.100518
C. Negrini , D. Luise , F. Correa , M. Mazzoni , A. Serra , A. Monteiro , P. Trevisi
{"title":"Effects of European authorised level of zinc from different sources on the physiology and intestinal ecosystem and performance of piglets weaned at different ages","authors":"C. Negrini ,&nbsp;D. Luise ,&nbsp;F. Correa ,&nbsp;M. Mazzoni ,&nbsp;A. Serra ,&nbsp;A. Monteiro ,&nbsp;P. Trevisi","doi":"10.1016/j.vas.2025.100518","DOIUrl":"10.1016/j.vas.2025.100518","url":null,"abstract":"<div><div>Early weaning can impact piglets’ gut health, and some forms of zinc (Zn) may mitigate this stress. This study evaluates the effect of authorised level of Zn, from different sources, on the performance and gut health of piglets weaned at different ages. At weaning (d0), 96 piglets weaned at either 21 (Early, E; 48) or 26 (Normal, N; 48) days were divided into 3 feeding groups: Zn sulphate (SO<sub>4</sub>), Zn glycinate (Gly) or a porous form of Zn oxide (Hi) to achieve 150 mg/kg of Zn. On d8 and d21, 8 piglets/group were slaughtered and oxidative status (blood), jejunal morphology, gene expression, microbiota, and volatile fatty acids and intestinal pH were assessed. The N group had a higher body weight throughout the study (<em>P</em> &lt; 0.001) and a higher gain to feed ratio from d7-d14 (<em>P</em> = 0.03). At d8, villus height and villus height:crypt depth ratio (VH:CD) tended to be higher in the N group (<em>P</em> &lt; 0.10). At d8, the Hi diet increased the abundance of the Lactobacillaceae (<em>P</em> = 0.04) in the E piglets. At d21, the Hi tended to increase VH:CD (<em>P</em> = 0.09) compared to SO<sub>4</sub> in N piglets; in the E groups fed SO<sub>4</sub> the crypt depth tended to be higher than Gly and Hi (<em>P</em> &lt; 0.10). Regardless the weaning age, the Gly tended to increase the expression of Glutathione Peroxidase-2 (<em>P</em> = 0.09) and Hi reduced the one of Solute Carrier Family-39 Member-4 (<em>P</em> = 0.07). Weaning age impacted piglets’ performance and gut physiology; feeding piglets different forms of Zn may positively improve gut health depending on weaning age.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100518"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of duplex crystal digital PCR (dPCR) assay for detection and differentiation of NDRV and MDRV 建立NDRV和MDRV双晶数字PCR (dPCR)检测和鉴别方法
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-08-21 DOI: 10.1016/j.vas.2025.100502
Huixin Liu , Chenchen Wang , Yang He , Qinxuan Wu , Jiayi Chen , Kaijun Wang
{"title":"Development of duplex crystal digital PCR (dPCR) assay for detection and differentiation of NDRV and MDRV","authors":"Huixin Liu ,&nbsp;Chenchen Wang ,&nbsp;Yang He ,&nbsp;Qinxuan Wu ,&nbsp;Jiayi Chen ,&nbsp;Kaijun Wang","doi":"10.1016/j.vas.2025.100502","DOIUrl":"10.1016/j.vas.2025.100502","url":null,"abstract":"<div><div>Muscovy duck reovirus (MDRV) and Novel duck reovirus (NDRV) are highly infectious diseases of waterfowl, causing significant harm to the global poultry industry. Early detection and diagnosis of NDRV and MDRV in clinical samples are crucial for effectively preventing and controlling these diseases. This study developed a duplex crystal digital PCR (dPCR) assay for the differential detection of NDRV and MDRV. Primer pairs and probes were designed specifically for the S3 genome of NDRV and the S2 genome of MDRV. To evaluate the method's performance, different reaction conditions were optimized, focusing on specificity, sensitivity, and reproducibility. The results showed that duplex crystal dPCR could accurately and differentially detect NDRV and MDRV, with a detection limit as low as 1 × 10⁻¹ copies/μl. It did not cross-react with other avian viruses, including duck Tembusu virus (DTMUV), H5 subtype avian influenza virus (H5 subtype AIV), H7 subtype AIV, H9 subtype AIV, Newcastle disease virus (NDV), infectious bronchitis virus (IBV), Egg drop syndrome virus (EDSV), Contagious bursal disease virus (IBDV, B87 strain), and duck Plague virus (DEV). The method also demonstrated good reproducibility, with intra-assay and inter-assay coefficients of variation (CV) both less than 8 %. For clinical application, 299 samples from coastal areas of Guangxi, China were tested. Duplex crystal dPCR detected NDRV and MDRV positive rates of 8.027 % and 6.020 %, respectively, with a co-infection rate of 1.672 %. The kappa values between duplex crystal dPCR and duplex quantitative polymerase chain reaction (qPCR) were 0.977 for NDRV and 1 for MDRV, indicating strong agreement. These findings confirm that the established duplex crystal dPCR is a specific, sensitive, and accurate method for detecting and quantifying NDRV and MDRV. This is the first report of using duplex crystal dPCR for NDRV and MDRV detection.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100502"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluations of milk yield, udder and teat morphometric traits of lactating goats reared in pastoral lowlands of the Bale zone, Ethiopia 埃塞俄比亚贝尔牧区低地饲养的泌乳山羊产奶量、乳房和乳头形态特征评价
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-20 DOI: 10.1016/j.vas.2025.100521
Mesay Guyo, Melaku Tareke
{"title":"Evaluations of milk yield, udder and teat morphometric traits of lactating goats reared in pastoral lowlands of the Bale zone, Ethiopia","authors":"Mesay Guyo,&nbsp;Melaku Tareke","doi":"10.1016/j.vas.2025.100521","DOIUrl":"10.1016/j.vas.2025.100521","url":null,"abstract":"<div><div>Udder and teat morphometrics provide a simple, low-cost tool to predict milk yield, showing positive correlations with yield and body condition, and serving as key selection criteria for improvement programs. Thus, the study was conducted to evaluate milk yield performances and udder and teat morphometric traits of lactating goats reared in the lowlands of the Bale Zone, Ethiopia. For this study, 270 clinically healthy lactating goats were selected from three districts highly inhabited by goats. Data on the daily milk yield (DMY), udder height (UH), udder width (UW), udder circumference (UC), udder depth (UD), teat depth (TD), teat length (TL), distance between the teats (DBT), teat circumference (TC), udder distance from ground (UDG), and udder volume (UV) of lactating does were measured early in the morning and evening before kids sucking among lactation stages, dam parity, birth type, and sex of kids. Data were analyzed using ANOVA, correlation, regression, and multivariate analysis. Results indicated that DMY, udder, and teat morphometric traits were significantly (<em>p</em> &lt; 0.05) affected among the studied districts except UV. The lactation stage effect was significant (<em>p</em> &lt; 0.0001) on DMY and UC; however, the effect was non-significant for UD, UDG, and TD. The doe parity effect was highly significant (<em>p</em> &lt; 0.0001) on TD and DMY, while it was non-significant (<em>p</em> &gt; 0.05) on UW, UD, UC, UDG, and UV. High milk yield in does enhanced the performance of second-parity, single-born, and male kids. UC, UV, and UW showed significant variations (<em>p</em> &lt; 0.0001) and exhibited moderate to high correlations with DMY, indicating strong positive associations for predicting milk yield performance. Therefore, presence of wide variation among all measured udder and teat morphometric traits and DMY among studied goat populations were potentials for selections to initiate community based breeding programs in lowlands of the Bale zone, Ethiopia.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100521"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fecal intestinal inflammatory biomarkers in piglets: analytical validation, homogeneity and stability studies 仔猪粪便肠道炎症生物标志物:分析验证、均匀性和稳定性研究
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-20 DOI: 10.1016/j.vas.2025.100523
Anna Pelegrí-Pineda , Raquel Pato , Raquel Peña , Júlia Suppi , Eudald Llauradó-Calero , David Solà-Oriol , Anna Bassols , Yolanda Saco
{"title":"Fecal intestinal inflammatory biomarkers in piglets: analytical validation, homogeneity and stability studies","authors":"Anna Pelegrí-Pineda ,&nbsp;Raquel Pato ,&nbsp;Raquel Peña ,&nbsp;Júlia Suppi ,&nbsp;Eudald Llauradó-Calero ,&nbsp;David Solà-Oriol ,&nbsp;Anna Bassols ,&nbsp;Yolanda Saco","doi":"10.1016/j.vas.2025.100523","DOIUrl":"10.1016/j.vas.2025.100523","url":null,"abstract":"<div><div>Analytical validation of biomarker assays as indicators of intestinal inflammatory processes is essential for the diagnosis, prognosis and monitoring of intestinal health. In the context of porcine intestinal health, feces are a sample of choice for determining inflammatory molecules released into the intestinal contents in pathological conditions. The advantage of this method is that it is non-invasive and samples can be collected easily, quickly and without causing stress. In this study, we validated the analytical procedures for measuring lipocalin-2 (LCN-2), myeloperoxidase (MPO) and adenosine deaminase (ADA) in porcine fecal samples. Validation was carried out by studying precision (intra- and inter-assay coefficients of variation), linearity under dilution, recovery, and the limits of detection and quantification. All three biomarkers fulfilled the required validation parameters. Furthermore, we analyzed the distribution of the three compounds and calprotectin (fCal) in the stools and found that all of them except LCN-2 were homogeneously distributed in the fecal sample. The stability of the compounds under different storage temperatures was assessed, and fCal was found to be the most stable parameter overall.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100523"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and evaluation of agroecosystem compartments as forage sources for free-ranging goats in smallholder farming systems of western Democratic Republic of Congo 刚果民主共和国西部小农农业系统中作为自由放养山羊饲料来源的农业生态系统区隔的鉴定和评价
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-16 DOI: 10.1016/j.vas.2025.100524
Alain Ndona, Anthony Kikufi Batoba, Eric Lutete, Bienvenu Kambashi Mutiaka, Charles-Henri Moulin, Yves Beckers, Jérôme Bindelle
{"title":"Identification and evaluation of agroecosystem compartments as forage sources for free-ranging goats in smallholder farming systems of western Democratic Republic of Congo","authors":"Alain Ndona,&nbsp;Anthony Kikufi Batoba,&nbsp;Eric Lutete,&nbsp;Bienvenu Kambashi Mutiaka,&nbsp;Charles-Henri Moulin,&nbsp;Yves Beckers,&nbsp;Jérôme Bindelle","doi":"10.1016/j.vas.2025.100524","DOIUrl":"10.1016/j.vas.2025.100524","url":null,"abstract":"<div><div>Free-ranging goats forage across multiple agroecosystem compartments, thereby contributing to nutrient redistribution. Despite their importance in nutrient flows, little is known about how these compartments support goat diets or about their spatial structure. This study addresses this gap by identifying, mapping, and characterising the agroecosystems exploited by free-ranging goats as forage sources and by assessing the contribution of each compartment to their daily diet. Twelve adult female goats from local herds were monitored during both the dry and rainy seasons for three consecutive days at four sites, using GPS collars combined with drone surveys and direct observations. Two observers were assigned to each goat: one, equipped with a stopwatch and tracking sheet, recorded feeding stations, forages consumed, and biting times; the other, using a Sony HDR-CX405 camera, documented biting behaviour.</div><div>Drone-based mapping revealed five distinct compartments of the foraging area: croplands (6.1 %–14.8 %), fallow lands (2.7 %–12.5 %), rangelands (36.4 %–69.7 %), residue supply areas (9.8 %–46.7 %), and hedgerows (0.1 %–5.4 %). The agroecosystem landscapes were highly fragmented (24–54 patches/ha) and exhibited SHDI values ranging from 0.94 to 1.19. Goats consumed 57 forage species, dominated by grasses (17 %) and legumes (9 %), with the remaining 74 % spanning 31 botanical families. The integration of GPS tracks with land-use maps and direct observations revealed that rangelands and agricultural lands were the primary contributors to free-ranging goats’ daily dry matter intake and forage species diversity. The marked heterogeneity and floristic richness of these agroecosystem compartments underpin their importance as key forage sources supporting smallholder free-range goat farming in western Democratic Republic of Congo.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100524"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence and associated factors of schistosomiasis among domestic ruminants in Ethiopia: a systematic review and meta-analysis 埃塞俄比亚国内反刍动物中血吸虫病的流行及其相关因素:一项系统综述和荟萃分析
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-28 DOI: 10.1016/j.vas.2025.100535
Yihenew Getahun Ambaw , Simachew Getaneh Endalamew , Ambachew Motbaynor Wubaye , Simegnew Adugna Kallu
{"title":"Prevalence and associated factors of schistosomiasis among domestic ruminants in Ethiopia: a systematic review and meta-analysis","authors":"Yihenew Getahun Ambaw ,&nbsp;Simachew Getaneh Endalamew ,&nbsp;Ambachew Motbaynor Wubaye ,&nbsp;Simegnew Adugna Kallu","doi":"10.1016/j.vas.2025.100535","DOIUrl":"10.1016/j.vas.2025.100535","url":null,"abstract":"<div><div>Schistosomiasis is a significant concern for animal and public health, particularly in low-income and rural areas with limited access to clean water and healthcare services. Evidence on the national pooled prevalence and risk factors of ruminant schistosomiasis in Ethiopia is limited, underscoring the need for further research to inform effective control strategies. This systematic review and meta-analysis was conducted in line with the PRISMA guidelines. An extensive review was conducted across five academic databases (PubMed, Google Scholar, ScienceDirect, Scopus, and Web of Science), with all searches completed between May 10, 2025, and June 1, 2025. Heterogeneity was assessed, and a random-effects model was used to determine the pooled prevalence of schistosomiasis in ruminants. The sources of variation were analyzed through subgroup analysis, meta-regression, and sensitivity analysis. Publication bias was assessed using funnel plots, Begg's test, and Egger’s test. A total of 33 studies were included in this meta-analysis, revealing a pooled prevalence of schistosomiasis in ruminants of 14.4 %, 95 % CI: [11.2-17.6]. The analysis demonstrated a substantial degree of heterogeneity among studies, with I² = 98.2 %, Q=1764.5 and p &lt; 0.001. Among the variables analyzed for heterogeneity, the species of domestic ruminant and geographical region were identified as significant risk factors. Subgroup analysis revealed that the prevalence of schistosomiasis varied by species: 15.9 %, 95 % CI: [12.5–19.3] in cattle, 8.4 %, 95 % CI: [0.0–20.3] in sheep, and 3.0 %, 95 % CI: [0.0–31.9] in goats. According to the geographical region-based subgroup meta-analysis, the Amhara region exhibited the highest prevalence among domestic ruminants at 18.6 %, 95 % CI: [15.4–21.7], followed by the Oromia region at 12.8 %, 95 % CI: [4.8–20.9]. This systematic review and meta-analysis highlight that schistosomiasis remains a persistent threat to domestic ruminants in Ethiopia. Thus, veterinary and public health professionals, research institutions, livestock owners, and policymakers are encouraged to collaborate in designing and implementing effective prevention and control strategies for schistosomiasis.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100535"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145474035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Allelic diversity of Blec2 gene in indigenous and local chickens and red junglefowl in Thailand: Implications for disease resistance 泰国本地鸡和地方鸡及红丛林鸡Blec2基因的等位基因多样性:对抗病的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-30 DOI: 10.1016/j.vas.2025.100532
Trifan Budi , Worapong Singchat , Nivit Tanglertpaibul , Thanyapat Thong , Thitipong Panthum , Aingorn Chaiyes , Narongrit Muangmai , Orathai Sawatdichaikul , Darren K Griffin , Prateep Duengkae , Yoichi Matsuda , Kornsorn Srikulnath
{"title":"Allelic diversity of Blec2 gene in indigenous and local chickens and red junglefowl in Thailand: Implications for disease resistance","authors":"Trifan Budi ,&nbsp;Worapong Singchat ,&nbsp;Nivit Tanglertpaibul ,&nbsp;Thanyapat Thong ,&nbsp;Thitipong Panthum ,&nbsp;Aingorn Chaiyes ,&nbsp;Narongrit Muangmai ,&nbsp;Orathai Sawatdichaikul ,&nbsp;Darren K Griffin ,&nbsp;Prateep Duengkae ,&nbsp;Yoichi Matsuda ,&nbsp;Kornsorn Srikulnath","doi":"10.1016/j.vas.2025.100532","DOIUrl":"10.1016/j.vas.2025.100532","url":null,"abstract":"<div><div>Although selective breeding significantly enhances production traits in commercial chickens, it often compromises their immune function. Indigenous chickens, however, typically exhibit strong disease resistance. The major histocompatibility complex plays a critical role in the adaptive immune responses to pathogens in chickens. The <em>Blec2</em> gene in the MHC-B region, which encodes a putative natural killer cell receptor, is a promising candidate that influences the early immune responses. Little, however, is known about polymorphisms of this gene in indigenous and local chicken breeds or red junglefowl in Thailand. In this study, polymorphisms in a partial fragment of exon 4 and intron 3 of <em>Blec2</em> were examined using targeted next-generation sequencing and genetic diversity analyses. Fourteen alleles and nine single nucleotide substitutions were identified; these included both silent and missense mutations, which may influence immune function. Notably, one allele, <em>Blec2*TH2</em>, referred to as haplotype 21, is reported to be strongly associated with resistance against the H5N1 virus. Purifying selection alongside stochastic processes were also observed in this gene fragment, indicating a strong potential for disease resistance. By contrast, <em>Blec2*TH13</em> allele referred to as haplotype 13, which was previously reported to correlate with 100% mortality rate for avian influenza, was detected in Nin Kaset breed. The study findings indicate the existence of diverse immune response mechanisms in indigenous and local chickens and red junglefowl in Thailand. These findings provide valuable insights that should be relevant for information for developing breeding programs using marker-assisted selection to enhance the immune resilience of commercial poultry stocks.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100532"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145474167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Global distribution and host range of enzootic bovine leukosis in ruminants: A systematic review and meta-analysis 反刍动物地方性牛白血病的全球分布和宿主范围:系统综述和荟萃分析
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-08-23 DOI: 10.1016/j.vas.2025.100499
Melkie Dagnaw , Getachew Alemu Yilhal , Bemrew Admassu , Yitayew Demessie
{"title":"Global distribution and host range of enzootic bovine leukosis in ruminants: A systematic review and meta-analysis","authors":"Melkie Dagnaw ,&nbsp;Getachew Alemu Yilhal ,&nbsp;Bemrew Admassu ,&nbsp;Yitayew Demessie","doi":"10.1016/j.vas.2025.100499","DOIUrl":"10.1016/j.vas.2025.100499","url":null,"abstract":"<div><div>Enzootic bovine leukosis (EBL) causes severe economic losses and is a contagious disease caused by the Bovine Leukemia Virus (BLV), belonging to the delta retrovirus of the Retroviridae family. Thus, this review aimed to estimate the global pooled prevalence of enzootic bovine leukosis and investigate its host range, focusing on cattle and sheep. We used seven databases which include PubMed, Science Direct, HINARI, Scopus, Google Scholar, Web of Science, and AJOL. The included studies (50 articles), conducted between 1992 and 2024, represent diverse geographic regions: Asia, North America, Africa, South America, and Europe. 346,917 animals were involved, of which 99,620 involved positive for BLV. The meta-analysis estimated a pooled BLV prevalence of 19% (95% CI: 16%–23%) using random effect model, though significant heterogeneity existed across studies (I² = 99.6%, p &lt; 0.001). Subgroup analysis revealed that the highest prevalence of EBL was in North America (43%), followed by Asia (17%), Africa (14%), South America (10%), and the lowest in Europe (4%). Analysis by publication year revealed that the prevalence of EBL was 32% after 2016, whereas it was 29% before 2016. Among animal species, beef cattle exhibited the highest prevalence (26%), followed by overall cattle (20%), sheep (19%), and dairy cattle (18%). Geographical and temporal trends revealed varying prevalence rates, with notable high rates in North America and lower rates in regions such as Europe. It is crucial that North America adopt stricter prevention programs, similar to those in Europe, to reduce transmission of EBL and its economic and health impacts on livestock.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100499"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of dietary supplementation with selenium in organic form and iodine in dairy goats and their transfer to milk and dairy products 饲粮中添加有机硒和碘对奶山羊的影响及其向牛奶和乳制品的转移
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-10 DOI: 10.1016/j.vas.2025.100520
I. Azorín , J. Madrid , S. Martínez-Miró , M.B. López , M. López , F. Hernández
{"title":"Effects of dietary supplementation with selenium in organic form and iodine in dairy goats and their transfer to milk and dairy products","authors":"I. Azorín ,&nbsp;J. Madrid ,&nbsp;S. Martínez-Miró ,&nbsp;M.B. López ,&nbsp;M. López ,&nbsp;F. Hernández","doi":"10.1016/j.vas.2025.100520","DOIUrl":"10.1016/j.vas.2025.100520","url":null,"abstract":"<div><div>This study evaluated the effects of dietary supplementation with selenium in organic form and iodine in dairy goats, aiming to obtain milk, cheese, and yogurt naturally enriched with these minerals and assessing the impact on animal health and performance. The control diet (CON) included 0.23 mg of inorganic selenium and 0.57 mg of iodine per kg of dry matter (DM). In contrast, the supplemented diet (SeI) included 0.46 mg of selenium (0.23 mg inorganic + 0.23 mg organic) and 5.68 mg of iodine per kg of DM. After 64 days of treatment, no adverse effects on health or productivity were observed. However, the SeI group exhibited elevated selenium and iodine concentrations in milk (p &lt; 0.001), with 1.73 times more selenium (3.93 μg/100 g vs. 2.27 μg/100 g) and over 4 times more iodine (98.0 μg/100 g vs. 21.7 μg/100 g) compared to the CON group. A similar enrichment was observed in cheese (p &lt; 0.001), where selenium content was approximately doubled (16.0 µg/100 g vs. 7.56 µg/100 g), and iodine quintupled (99.4 µg/100 g vs. 20.1 µg/100 g) in the SeI group. More than a 4-fold increase in iodine content was also observed in yogurt from the SeI group (106 µg/100 g vs. 24.4 µg/100 g; p &lt; 0.001). Notably, selenium and iodine treatment did not affect the general characteristics or quality of milk and dairy products, supporting their use as a strategy to enhance the nutritional value of goat milk and derivatives.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100520"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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