Analyzing the expression of the transcriptome in adipose tissue of fat- and thin-tailed sheep

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Sana Farhadi , Karim Hasanpur , Jalil Shodja Ghias , Valiollah Palangi , Maximilian Lackner
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引用次数: 0

Abstract

Significant efforts have been made to understand how fat deposition in sheep tail is regulated in genetic, transcriptomic, physiologic, biochemical, and metabolic levels in order to elucidate the complex mechanisms underlying the energy storage, lipid metabolism in adipose tissue, adaptability to harsh environments, and evolutionary domestication. Through RNA-seq data analysis, we are able to compare the gene expression of fat-tailed sheep versus thin-tailed sheep breeds in an acceptable resolution at transcriptome level. The purpose of this study was to compare the transcriptomes of Ghezel (fat-tailed) and Zel (thin-tailed) sheep. Total RNA from subcutaneous and tail tissue samples from healthy lambs was sequenced (150b PE) to identify differentially expressed genes (DEGs) between the two mentioned tissues and between the Ghezel and Zel sheep breeds. Further downstream pathway and network analyses were conducted afterwards. The results uncovered the association of the most important DEGs such as CAV1, ALB, and SOCS3 with cellular signaling pathways of lipids metabolism. It seems that the SOCS3 gene plays an important role in the differential deposition of lipid in the tails of two phenotypically different sheep breeds. Although the detail of gene expression in the tail and subcutaneous tissues of two morphologically different breeds was decoded here, to fully understand how differential expression of the SOCS3 gene affects the fat synthesis, further studies are needed.

分析肥尾绵羊和瘦尾绵羊脂肪组织中转录组的表达情况
为了了解绵羊尾部的脂肪沉积是如何在遗传、转录组、生理、生化和代谢水平上进行调控的,以阐明能量储存、脂肪组织的脂质代谢、对恶劣环境的适应性以及进化驯化的复杂机制,我们做出了巨大的努力。通过 RNA-seq 数据分析,我们能够以可接受的分辨率在转录组水平上比较肥尾绵羊与瘦尾绵羊品种的基因表达。本研究的目的是比较 Ghezel(肥尾)绵羊和 Zel(细尾)绵羊的转录组。对健康羔羊皮下和尾部组织样本的总 RNA 进行测序(150b PE),以确定上述两种组织之间以及 Ghezel 和 Zel 羊品种之间的差异表达基因(DEGs)。随后进行了进一步的下游通路和网络分析。结果发现最重要的 DEGs(如 CAV1、ALB 和 SOCS3)与脂质代谢的细胞信号通路有关。看来,SOCS3 基因在两个表型不同的绵羊品种尾部脂质沉积差异中起着重要作用。虽然本文解密了两个形态不同品种的绵羊尾部和皮下组织中基因表达的细节,但要全面了解 SOCS3 基因的不同表达如何影响脂肪的合成,还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary and Animal Science
Veterinary and Animal Science Veterinary-Veterinary (all)
CiteScore
3.50
自引率
0.00%
发文量
43
审稿时长
47 days
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