Veterinary and Animal Science最新文献

{"title":"Phytochemical profile and proteomic landscape associated with antineoplastic activity of Zingiber cassumunar Roxb. Essential oil in canine melanoma","authors":"Peerawit Chongrattanameteekul,&nbsp;Chiao-Hsu Ke,&nbsp;Chen-Si Lin,&nbsp;Phongsakorn Chuammitri,&nbsp;Pitchaya Matchimakul,&nbsp;Chompunut Lumsangkul,&nbsp;Siriporn Okonogi,&nbsp;Raktham Mektrirat","doi":"10.1016/j.vas.2026.100840","DOIUrl":"10.1016/j.vas.2026.100840","url":null,"abstract":"<div><div>Canine malignant melanoma is an aggressive neoplasm with limited remedial options. Therefore, the development of novel antineoplastic agents is urgently needed. This study investigated the medicinal potential and molecular mechanisms of <em>Zingiber cassumunar</em> Roxb. essential oil (CEO) against canine melanoma. Phytochemical profiling via GC–MS identified 22 compounds, with monoterpenoids (90.99%) predominating, specifically sabinene (53.25%) and terpinen-4-ol (17.99%). CEO exhibited potent, concentration-dependent cytotoxicity across four canine melanoma cell lines, with a mean IC₅₀ of approximately 0.006% v/v. Quantitative proteomic mapping of CEO-treated M5 cells identified a total of 222 differentially expressed proteins associated with systemic cellular collapse. Key mechanisms included significant genotoxic stress (upregulation of H2AX and histone variants), endoplasmic reticulum stress (suppression of CALR and HSPB1), and the activation of a coordinated necroptosis/mitophagy axis (modulation of Drp1, VDAC, and p62). Protein-protein interaction network analysis further identified SLC2A1 as a central metabolic hub with high-confidence connectivity to conventional chemotherapies, suggesting that CEO-induced metabolic disruption converges onto core pathways targeted by 5-fluorouracil and imatinib. These findings suggest that CEO induces complex cell death signatures through coordinated nuclear and organelle stress. Ultimately, it should be considered as a promising candidate for future translational research and functional validation.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"33 ","pages":"Article 100840"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial rearing with milk replacers of varying fat and total solids compared with natural suckling: Pre- and post-weaning performance of lambs","authors":"Ameneh Akbarizadeh,&nbsp;Ebrahim Ghasemi,&nbsp;Meysam Gholami,&nbsp;Bahareh Dolatkhah","doi":"10.1016/j.vas.2026.100839","DOIUrl":"10.1016/j.vas.2026.100839","url":null,"abstract":"<div><div>This study evaluated the effects of increasing total solids (TS) and fat concentration in milk replacer (MR) on the performance of artificially reared lambs compared with naturally suckled lambs. Forty-eight 3-day-old Shal lambs were assigned to natural suckling (EWE; 6.37% fat, 17.4% TS) or one of three MR treatments: low-fat–low-solids (LF-LS; 3.56% fat, 16.3% TS), low-fat–high-solids (LF-HS; 4.27% fat, 19.6% TS), or high-fat–high-solids (HF-HS; 4.95% fat, 19.6% TS). Increasing TS raised the milk protein concentration to a level comparable to that of ewe milk (4.8%), while partial replacement of lactose with fat reduced osmolality in HF-HS relative to LF-HS (466 vs. 506 mOsm/kg). Lambs were fed milk until day 62 and monitored until day 103. Lambs fed LF-HS and HF-HS consumed more protein and metabolizable energy than those fed LF-LS, resulting in improved pre- and postweaning average daily gain and skeletal growth. Furthermore, LF-LS lambs exhibited lower serum cholesterol, a tendency toward lower glucose, and higher β-hydroxybutyrate concentrations at weaning, indicating a less favorable energy status. Although HF-HS increased nutrient intake and hip width compared with LF-HS, both high-solids formulations achieved growth comparable to that of EWE. Nutrient digestibility, diarrhea days, and serum concentrations of insulin, cortisol, and thyroxine were unaffected, whereas serum total protein was greater in EWE than in the MR groups. Despite the associated increase in osmolality, increasing MR solids from 16.3% to 19.6%, particularly in HF-HS, improved nutrient intake and supported growth comparable to natural suckling without adversely affecting the assessed health indicators.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"33 ","pages":"Article 100839"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic parameter estimation of novel international show-jumping indicators in horses","authors":"Michela Ablondi,&nbsp;Tommaso Danese,&nbsp;Riccardo Moretti,&nbsp;Vittoria Asti,&nbsp;Laura Ozella,&nbsp;Alberto Sabbioni,&nbsp;Emanuela Valle,&nbsp;Andrea Summer","doi":"10.1016/j.vas.2026.100829","DOIUrl":"10.1016/j.vas.2026.100829","url":null,"abstract":"<div><div>Genetic improvement of show-jumping competition results has a clear breeding goal which is to produce horses that can repeatedly clear technically demanding courses at maximum height, resulting in placement results. However, how to achieve this, and thus how to accurately choose breeding candidates, is far from straightforward due to the heterogeneity of competition outcomes. In this study, we analyzed Fédération Équestre Internationale (FEI) competition records from 19,232 horses together with pedigree data to evaluate novel phenotypes suitable for international genetic evaluations. Traditional traits (maximum height jumped, total prize money won, number of competitions) were analyzed together with composite ratios (clear-round ratio and placement ratio), modeled both across career and within 5-cm FEI height categories (1.20–1.60 m). Using animal models and binomial threshold models in ASReml, we estimated heritability, repeatability, and genetic correlations. Maximum height achieved in career showed moderate heritability (h² = 0.22), while earnings and number of competitions displayed low heritability and modest repeatability. Composite ratios modeled over career had low heritability (h²obs = 0.02–0.08) and moderate repeatability, whereas analyses within height categories revealed substantially higher heritabilities (h²obs up to 0.59), especially in mid-level classes (1.35–1.45 m). Genetic correlations were high between adjacent heights, but weaker between the lowest and elite levels, suggesting partly distinct genetic determinants across competition classes. These findings indicate that FEI-restricted composite phenotypes, especially mid-height ratios, provide informative and heritable indicators for breeding programs.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"33 ","pages":"Article 100829"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field performance of a point-of-care PCR platform for the detection of influenza A virus in growing pigs","authors":"Everett W. Dalesio ,&nbsp;Ting-Yu Cheng ,&nbsp;Andrew S. Bowman ,&nbsp;Sylvester Ochwo ,&nbsp;Rachel A. Schambow ,&nbsp;Maria Sol Pérez ,&nbsp;Andres Perez ,&nbsp;Andréia G. Arruda","doi":"10.1016/j.vas.2026.100594","DOIUrl":"10.1016/j.vas.2026.100594","url":null,"abstract":"<div><div>Point-of-care (POC) testing involves conducting diagnostic assays near patients to enable rapid decision-making and timely intervention. To date, evaluation of POC devices under field conditions in veterinary medicine has been limited. The objective of this study was to describe the use and performance of a POC system in the field and with varying sample conditions using influenza A virus in swine. Seventy oral fluid samples were collected from ten swine farms and aliquoted into five treatments: portable PCR performed on farm (FARM), while driving (RIDE), after 24 h incubation at room temperature (RT), on ice (ICE), and as a gold standard, samples were also submitted for testing at the Ohio Animal Disease Diagnostic Laboratory (LAB). Influenza A virus was detected at least in one oral fluid sample for six out of the ten farms sampled according to gold standard results; within-herd prevalence in positive farms varied from 16.7 to 100.0%. POC system’s Ct values were statistically associated with the gold standard results. POC tests showed high specificity, with median estimates ranging from 0.954 to 0.958 and 95% credible intervals (CrIs) consistently above 0.91. Sensitivity of POC tests varied more substantially, with the lowest observed in the ICE setting (median 0.805; 95% CrI: 0.607–0.945) and the highest in the RT setting (median 0.926; 95% CrI: 0.766–0.995). Our study showed that the POC PCR system was a promising method for on-site diagnostic testing, providing results consistent with official laboratory results.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"32 ","pages":"Article 100594"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146193195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ’Salivary responses to an experimental activation of the HPA axis in pigs: Effects of ACTH on analytes related to stress, immune system, inflammation, redox status and metabolism’ [Veterinary And Animal Science 32 (2026)100650]","authors":"María Botía ,&nbsp;José Joaquín Cerón ,&nbsp;Silvia Martínez-Subiela ,&nbsp;Alba Ortín-Bustillo ,&nbsp;Alberto Muñoz-Prieto ,&nbsp;Damián Escribano","doi":"10.1016/j.vas.2026.100712","DOIUrl":"10.1016/j.vas.2026.100712","url":null,"abstract":"","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"32 ","pages":"Article 100712"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148184372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary inclusion of beetroot (Beta vulgaris): Impacts on egg production, egg quality, and physiological status of commercial laying hens","authors":"Mansur Ahmed ,&nbsp;Kanon Das ,&nbsp;Momotaj Hossen ,&nbsp;Mohammad S Ataher ,&nbsp;Jannatul M Momu ,&nbsp;Md Aktaruzzaman ,&nbsp;Mowdudul H. Talha ,&nbsp;Abu Taher ,&nbsp;Mohammad A Mamun ,&nbsp;Sonjoy Sarkar ,&nbsp;Shad Mahfuz","doi":"10.1016/j.vas.2025.100559","DOIUrl":"10.1016/j.vas.2025.100559","url":null,"abstract":"<div><div>This study evaluated the effects of dietary beet root powder (BRP; <em>Beta vulgaris</em>) supplementation on egg production, egg quality, and physiological status of commercial laying hens. A total of 108 ISA Brown hens (40 weeks old) were randomly allocated into three dietary treatments: a control group (basal diet without BRP), BRP-0.5 % (basal diet with 0.5 % BRP), and BRP-1 % (basal diet with 1.0 % BRP) for eight weeks. Each treatment included 36 hens, divided into four replicates of nine birds. Supplementation with 1 % BRP significantly (<em>P</em> &lt; 0.05) increased egg weight and egg mass compared with the control, without altering hen-day egg production, external, and internal quality of eggs. Meanwhile, BRP-1 % improved the feed conversion ratio (<em>P</em> &lt; 0.05) without affecting the feed intake. Fatty acid analysis revealed a significant reduction (<em>P</em> &lt; 0.05) in total saturated fatty acids, particularly in palmitic acid (C16:0) in the BRP-1 % group. Moreover, BRP-1 % showed a significant increase (<em>P</em> &lt; 0.05) in linolenic acid (C18:3, omega-3) and total omega-3 fatty acids relative to the other groups. Amino acid profiling showed the highest rise in glutamic acid, with BRP-1 % significantly higher (<em>P</em> &lt; 0.05) than the control. Additionally, BRP-1 % supplementation significantly reduced (<em>P</em> &lt; 0.05) serum total cholesterol and triglyceride levels contrast to the control and BRP-0.5 % groups. Finally, this study suggests that 1 % BRP supplementation can be applied in laying hen diets to improve egg quality, egg yolk fatty acid profile, and lipid metabolism of laying hens without compromising health and egg production.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100559"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary oils modulate inflammatory and oxidative responses in pigs: Associations with hepatic gene expression","authors":"Fernanda Nery Ciconello ,&nbsp;Christopher Keith Tuggle ,&nbsp;Simara Larissa Fanalli ,&nbsp;Bruna Pereira Martins da Silva ,&nbsp;Luciana Salles de Freitas ,&nbsp;Flaviana Miranda Gonçalves ,&nbsp;Cristina Tschorny Moncau-Gadbem ,&nbsp;Ana Sofia Martelli Chaib Saliba ,&nbsp;Felipe André Oliveira Freitas ,&nbsp;Izally Carvalho Gervásio ,&nbsp;Severino Matias de Alencar ,&nbsp;Júlio César de Carvalho Balieiro ,&nbsp;James Eugene Koltes ,&nbsp;Aline Silva Mello Cesar","doi":"10.1016/j.vas.2026.100583","DOIUrl":"10.1016/j.vas.2026.100583","url":null,"abstract":"<div><div>Pigs are valuable animal models for metabolism. The impact of fatty acids (FAs) on lipid metabolism and inflammatory responses must be considered when analyzing the types of FAs present in edible oils. This study aimed to evaluate how diets containing soybean oil (SO), canola oil (CO), or fish oil (FO) influence cytokine levels and oxidative stress in liver and blood serum, reflecting local and systemic inflammation. Additionally, correlations with hepatic gene expression were assessed through weighted gene co-expression analysis. Twenty-seven male pigs were fed soybean meal and corn-based diets supplemented with 3% SO, CO, or FO for 98 days. Cytokines, including interleukin (IL)-10, interferon (IFN)-γ, IL-1β, IL-6, IL-18, and tumor necrosis factor (TNF)-α, were quantified in serum and liver using Enzyme-Linked Immunosorbent Assay (ELISA). Oxidative status in serum was evaluated using the ORAC assay and hypochlorous acid scavenging capacity. The CO diet increased IFN-γ in liver tissue and enhanced hypochlorous acid scavenging capacity, while the SO diet elevated IL-10 levels. Gene co-expression analysis revealed associations between cytokine levels and hepatic gene modules. The SO diet correlated positively with modules linked to IL-10 and TNF-α. The CO diet was associated with increased IFN-γ and decreased IL-6 and IL-18. The FO diet was negatively correlated with TNF-α. These results suggest that dietary oils modulate inflammatory and oxidative responses in pigs, with distinct patterns depending on fatty acid composition. The findings contribute to understanding how specific lipid profiles influence immune regulation and oxidative balance in metabolic studies using swine models.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100583"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From rumen to milk: Dietary polyphenols in dairy cows—A critical review","authors":"Lucrezia Forte ,&nbsp;Nives Parabita ,&nbsp;Marta Santoro ,&nbsp;Francesco Longobardi ,&nbsp;Giuseppe Natrella ,&nbsp;John Quiñones ,&nbsp;Eric N. Ponnampalam ,&nbsp;Igor Tomasevic ,&nbsp;Pasquale De Palo ,&nbsp;Aristide Maggiolino","doi":"10.1016/j.vas.2026.100569","DOIUrl":"10.1016/j.vas.2026.100569","url":null,"abstract":"<div><div>Polyphenols represent a large and structurally diverse family of plant secondary metabolites with bioactive properties. In ruminants, these compounds can influence rumen fermentation, microbial ecology, and nutrient metabolism, offering potential benefits for animal health, productivity, and environmental sustainability. This review synthesizes evidence on the fate of dietary polyphenols in dairy cows from ingestion to their possible secretion into milk. It outlines the main dietary sources and classes of polyphenols, their microbial biotransformations in the rumen, and subsequent host metabolism involving absorption, conjugation, and systemic circulation. Particular attention is given to the mechanisms of mammary uptake and secretion, where most compounds appear as conjugated metabolites such as glucuronides, sulfates, and urolithins rather than parent forms. Although the transfer efficiency from feed to milk is typically low, consistent detection of isoflavone derivatives, phenyl-γ-valerolactones, urolithins, and hippuric acid demonstrates the feasibility of diet-to-milk modulation. Factors affecting bioavailability and transfer include polyphenol structure, dietary matrix, dose, rumen microbiota composition, animal physiology, and feed processing. Advances in high-resolution analytical techniques have improved the characterization of these low-abundance metabolites, yet large variability among studies persists. I<em>n vivo</em> studies indicate that polyphenol-derived metabolites in bovine milk occur at low ng/mL to low µg/mL levels, with compounds such as equol, enterolactone, urolithins, phenyl-γ-valerolactones and phenolic acids typically detected in the sub-micromolar range. Overall, dietary polyphenols offer promising opportunities to improve ruminant health and produce milk with enhanced functional quality, but quantitative and mechanistic research is still required to optimize feeding strategies and understand their contribution to milk bioactivity.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100569"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of sterility testing procedures for laboratory animal rodent diets","authors":"Jonathan W. Weeks,&nbsp;Jacqueline Locklear,&nbsp;Tanya E. Whiteside,&nbsp;David M. Kurtz","doi":"10.1016/j.vas.2026.100570","DOIUrl":"10.1016/j.vas.2026.100570","url":null,"abstract":"<div><div>The United States Food and Drug Administration (FDA) and the United States Department of Agriculture (USDA) recommend grinding or stomaching food for human consumption prior to Salmonella testing. The Quality Assurance Laboratory (QAL) at the National Institute of Environmental Health Sciences (NIEHS) routinely tests all incoming, unsterilized rodent feed for <em>Salmonella spp</em>. and total microbial load prior to use. Currently, the QAL cultures intact feed pellets in sterile Thioglycolate enrichment broth that sits stationary in the incubator for seven days. Under these conditions, a natural ingredient pelleted feed does not break down thoroughly possibly preventing detection of viable microbes at the pellet center. The purpose of this study was to determine if grinding pelleted rodent feed improves the sensitivity of microbial detection. Our study compared bacterial growth from an unautoclaved and autoclaved, natural ingredient rodent diet (NIH-31) processed as intact pellets or ground feed. Our results indicate that grinding unautoclaved feed for total aerobic bacteria testing does increase the sensitivity of microbial detection. However, when the feed is appropriately sterilized, grinding the feed provides no added benefit.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100570"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prokaryotic expression of the giant panda VEGFA gene and the preparation and characterization of its monoclonal antibodies","authors":"Xiangyu Liu ,&nbsp;Feiping Li ,&nbsp;Shenfei Wang ,&nbsp;Mengshi Zhang ,&nbsp;Xianbiao Hu ,&nbsp;Yuliang Liu ,&nbsp;Rong Hou ,&nbsp;Kailai Cai","doi":"10.1016/j.vas.2025.100551","DOIUrl":"10.1016/j.vas.2025.100551","url":null,"abstract":"<div><div>Vascular endothelial growth factor A (VEGFA) plays a crucial role in angiogenesis and is closely associated with two major challenges in giant panda (<em>Ailuropoda melanoleuca</em>) conservation: reproductive disorders and cancer. To support the development of diagnostic and therapeutic tools for this vulnerable species, this study aimed to generate and characterize monoclonal antibodies (mAbs) specific to giant panda VEGFA. The VEGFA gene fragment (35–517) was cloned and expressed as a recombinant protein in <em>Escherichia coli</em>, which was used as an immunogen. Through hybridoma technology, four stable mAb-secreting cell lines were established, all producing IgG2b subclass antibodies. The obtained mAbs were first screened and characterized by indirect enzyme-linked immunosorbent assay (ELISA) using the recombinant VEGFA protein. Their specific recognition of the native VEGFA protein in various giant panda tissues and cells was subsequently confirmed by Western blot analysis. In conclusion, this study successfully generated mAbs against giant panda VEGFA, providing a valuable reagent for future studies on pregnancy complications and cancer in this species.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100551"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145697939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}