Veterinary and Animal Science最新文献

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Robust BICP0-gB indirect ELISA for the accurate diagnosis of bovine alphaherpesvirus 1 infections 准确诊断牛甲疱疹病毒1型感染的强有力的BICP0-gB间接ELISA方法
IF 1.9
Veterinary and Animal Science Pub Date : 2025-09-10 DOI: 10.1016/j.vas.2025.100507
Sen Zhang , Liyuan Song , Xin Yin , Changmin Hu , Yingyu Chen , Aizhen Guo
{"title":"Robust BICP0-gB indirect ELISA for the accurate diagnosis of bovine alphaherpesvirus 1 infections","authors":"Sen Zhang ,&nbsp;Liyuan Song ,&nbsp;Xin Yin ,&nbsp;Changmin Hu ,&nbsp;Yingyu Chen ,&nbsp;Aizhen Guo","doi":"10.1016/j.vas.2025.100507","DOIUrl":"10.1016/j.vas.2025.100507","url":null,"abstract":"<div><div>Bovine alphaherpesvirus 1 (BoHV-1) represents a significant threat to the cattle industry, emphasizing the need for reliable diagnostic tools that facilitate effective disease management. Current diagnostic methods, including virus neutralization tests (VNTs), are often complex and labor intensive, but existing antibody detection assays may lack sufficient accuracy. In this study, we developed a novel indirect enzyme-linked immunosorbent assay (iELISA) utilizing both BICP0 and gB proteins to enhance the detection of BoHV-1 infections. The inclusion of BICP0, a pivotal protein during the early stages of viral infection, markedly improved the assay's specificity and sensitivity. The BICP0-gB iELISA exhibited a high degree of concordance with the VNT, demonstrating superior sensitivity and specificity. Preliminary clinical evaluations revealed a real prevalence of 40.3 % (95 % CI: 33.1 %-48.0 %) in serum samples from yaks in Qinghai Province, which aligns with the documented prevalence of BoHV-1 in the region. These results underscore that BICP0-gB iELISA is a robust and reliable diagnostic tool for the differential diagnosis of BoHV-1, providing a cost-effective and efficient solution for high-throughput screening in the livestock industry.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100507"},"PeriodicalIF":1.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of triptorelin and single fixed-time artificial insemination on productive and reproductive performance of hyperprolific sows 雷公藤素和单次定时人工授精对高产母猪生产和繁殖性能的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-30 DOI: 10.1016/j.vas.2025.100505
María Victoria Falceto , David Reyero , María Teresa Tejedor , Paul Renaud , Ana Mateu , Antonio Lafuente , Cristina Bonastre , Alicia Laborda , Ana Maria Garrido , Olga Mitjana
{"title":"Role of triptorelin and single fixed-time artificial insemination on productive and reproductive performance of hyperprolific sows","authors":"María Victoria Falceto ,&nbsp;David Reyero ,&nbsp;María Teresa Tejedor ,&nbsp;Paul Renaud ,&nbsp;Ana Mateu ,&nbsp;Antonio Lafuente ,&nbsp;Cristina Bonastre ,&nbsp;Alicia Laborda ,&nbsp;Ana Maria Garrido ,&nbsp;Olga Mitjana","doi":"10.1016/j.vas.2025.100505","DOIUrl":"10.1016/j.vas.2025.100505","url":null,"abstract":"<div><div>Single fixed-time artificial insemination (SFTAI) provides a streamlined approach to improving reproductive management. We evaluated the impact of incorporating triptorelin acetate (GnRH agonist) into a SFTAI protocol on a commercial farm. Hyperprolific sows not in estrus by day 4 post-weaning were randomly assigned to two groups. Group-1 (G1; <em>n</em> = 243) received no triptorelin treatment and daily post-cervical artificial inseminations (PCAI) based on estrus detection (2.59±0.034 inseminations/sow). Group-2 (G2; <em>n</em> = 249) received a single intravaginal dose of triptorelin (0.2 mg) at 96 ± 2 h post-weaning, then one PCAI 22 ± 2 h later. No significant differences (<em>P</em> &gt; 0.05) between groups in conception rate (G1: 98.8 %; G2: 97.6 %), farrowing rate (G1:95.1 %; G2: 94.4 %) or litter characteristics (total born, live-, stillborn and mummified piglets). Both groups showed similar delivery batch durations (<em>P</em> = 0.414). Distribution of deliveries during the delivery period was similar (<em>P</em> = 0.455). Induction of labor was need more frequently in G1 (<em>P</em> &lt; 0.001). G2 had a higher proportion of sows with shorter gestations (112–115 days: 166/235, 70.6 %) compared to G1 (112–115 days: 82/231; 35.5 %, <em>P</em> &lt; 0.001). A significantly higher proportion of G2 piglets received longer lactation (27 - 33 days) compared to G1 (2210/3023=73.1 % vs 2774/4395=63.1 %; <em>P</em> = 0.032). G1 had a higher proportion of low-weight piglets (≤4.0 kg; 716/43950= 16.3 % vs.435/3023= 14.4 %; while G2 had more heavy piglets (≥6.5 kg; 1228/3023=40.5 % vs.1675/4395= 38.1 %) (<em>P</em> = 0.027). Observed differences in lactation length could be responsible for these weaning weight differences, rather than triptorelin treatment itself. This protocol did not worsen productive and reproductive performances.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100505"},"PeriodicalIF":1.9,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of encapsulated Averrhoa carambola L. leaf extract on the growth performance, haemato-biochemical parameters, gut health, and antioxidant activity in broiler chickens 包封杨桃叶提取物对肉鸡生长性能、血液生化指标、肠道健康和抗氧化活性的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-29 DOI: 10.1016/j.vas.2025.100504
Binti Ma’rifah, Edjeng Suprijatna, Dwi Sunarti, Luthfi Djauhari Mahfudz, Sri Kismiati, Teysar Adi Sarjana, Rina Muryani, Hanna Dzawish Shihah, Nur Maulida Wahyuni, Sugiharto Sugiharto
{"title":"Effect of encapsulated Averrhoa carambola L. leaf extract on the growth performance, haemato-biochemical parameters, gut health, and antioxidant activity in broiler chickens","authors":"Binti Ma’rifah,&nbsp;Edjeng Suprijatna,&nbsp;Dwi Sunarti,&nbsp;Luthfi Djauhari Mahfudz,&nbsp;Sri Kismiati,&nbsp;Teysar Adi Sarjana,&nbsp;Rina Muryani,&nbsp;Hanna Dzawish Shihah,&nbsp;Nur Maulida Wahyuni,&nbsp;Sugiharto Sugiharto","doi":"10.1016/j.vas.2025.100504","DOIUrl":"10.1016/j.vas.2025.100504","url":null,"abstract":"<div><div>This study aimed to evaluate the effect of encapsulated <em>Averrhoa carambola</em> L<em>.</em> leaf extract (EAC) as a phytobiotic feed additive on the growth performance, haemato-biochemical parameters, gut health, and antioxidant activity in broiler chickens. A total of 280 one-day-old chicks were allocated to four treatments with seven replicates and 10 broilers per replicate (CON: control group/basal diet; EAC1: basal diet supplemented with 500 mg/kg EAC; EAC2: basal diet supplemented with 1000 mg/kg EAC; EAC3: basal diet supplemented with 1500 mg/kg EAC. Growth performance was significantly enhanced (<em>P</em> &lt; 0.05), as evidenced by increased body weight gain (days 15–21) and feed efficiency (days 15–35) in all treatment group compared with the control group. Haemato-biochemical parameters were positively influenced, with leukocytes and lymphocyte levels being significantly lower (<em>P</em> &lt; 0.05) in the EAC2 group than in the control group. The uric acid concentration was significantly reduced (<em>P</em> &lt; 0.05) in EAC1 and EAC3 groups compared to the control group. Gut health indicator improved, including the relative weight of the small intestine, liver, abdominal fat, length of duodenum and jejunum, weight and length ratio of the jejunum and ileum, ileum crypt depth, and lactic acid bacteria count in the ileum, were significantly higher (<em>P</em> &lt; 0.05) in the EAC1 group than in the others groups. In conclusion, the encapsulated <em>Averrhoa carambola</em> L. leaf extract is beneficial for enhancing growth performance, haemato-biochemicals parameters, and gut health in broiler chickens.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100504"},"PeriodicalIF":1.9,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a TaqMan probe-based dual real time PCR assay for the identification of NADC34-like PRRSV 基于TaqMan探针的双实时PCR方法鉴定nadc34样PRRSV的建立
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-27 DOI: 10.1016/j.vas.2025.100503
Shanshan Han , Tao Qin , Taichun Chen , Xiaocheng Xing , Qiang Zhang
{"title":"Development of a TaqMan probe-based dual real time PCR assay for the identification of NADC34-like PRRSV","authors":"Shanshan Han ,&nbsp;Tao Qin ,&nbsp;Taichun Chen ,&nbsp;Xiaocheng Xing ,&nbsp;Qiang Zhang","doi":"10.1016/j.vas.2025.100503","DOIUrl":"10.1016/j.vas.2025.100503","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus that induces reproductive disorders in sows and respiratory diseases in growing pigs. Recently, the NADC34-like strain of PRRSV has become more prevalent, with outbreaks occurring across pig farms in China. However, a reliable diagnostic method for the clinical detection of this strain has been absent. This study developed a TaqMan probe-based dual real-time quantitative PCR assay targeting the M and GP5 genes to specifically identify the NADC34-like PRRSV strain. The assay exhibited high specificity, detecting exclusively the NADC34-like strain without cross-reactivity with other PRRSV strains. The detection limits for pMD-M and pMD-GP5 plasmids were 2.67 × 10² and 1.35 × 10¹ copies/μL, respectively, indicating high assay sensitivity. The assay also demonstrated excellent reproducibility, with coefficient of variation (CV) values for both recombinant plasmids below 2 %. Among 251 clinical samples, 27 tested positive for NADC34-like PRRSV. This study establishes an accurate, sensitive, and reliable TaqMan dual real-time PCR assay for detecting NADC34-like PRRSV, offering a valuable tool for clinical diagnostics and outbreak management in pig farms.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100503"},"PeriodicalIF":1.9,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Typing identification and biological characterization of Clostridium perfringens toxins associated with lamb dysentery in Xizang sheep 西藏羊羊痢产气荚膜梭菌毒素分型、鉴定及生物学特性研究
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-25 DOI: 10.1016/j.vas.2025.100501
Zhuo Ma, Yuan Zhenjie
{"title":"Typing identification and biological characterization of Clostridium perfringens toxins associated with lamb dysentery in Xizang sheep","authors":"Zhuo Ma,&nbsp;Yuan Zhenjie","doi":"10.1016/j.vas.2025.100501","DOIUrl":"10.1016/j.vas.2025.100501","url":null,"abstract":"<div><div>The objectives of this study were to investigate the etiology of morbidity and mortality in Tibetan sheep from a township in Shannan, Tibet, and to characterize the biological characteristics of the causative pathogen, thereby providing a scientific basis for effective disease prevention and control. Pathological specimens were aseptically collected from deceased animals, and the pathogen was isolated and cultured based on clinical presentations. Identification of the isolate involved morphological observation, 16S rRNA PCR amplification and sequencing, biochemical assays, toxin gene typing, Kirby-Bauer disk diffusion antimicrobial susceptibility testing, and antibiotic resistance genes screening. On tryptose sulfite cycloserine (TSC) agar, the organism formed black, circular colonies. Gram staining revealed short, straight, Gram-positive bacilli arranged singly or in pairs, consistent with the <em>Clostridium perfringens</em> . 16S rRNA gene sequence alignment showed &gt;99 % identity to reference <em>C. perfringens</em> sequences in the NCBI database.</div><div>Confirming the strain asC. <em>perfringens</em>. Biochemical tests indicated positive reactions for glucose, Biochemical assays were positive for glucose, lactose, and maltose fermentation; gelatin hydrolysis; oxidase activity; nitrate reduction;hydrogen sulfide production; and ornithine decarboxylase, but negative results for indole production, mannitol and sorbitol fermentation, and urea hydrolysis, matching known traits of <em>C. perfringens</em>. . Toxin gene PCR detected <em>cpa,cpb</em>, and <em>etx,</em> but not <em>iap, cpe</em>, or <em>netB</em>, identifying the isolate as <em>C. perfringens type B</em>. Antimicrobial susceptibility testing revealed high sensitivity to sulfadiazine, norfloxacin, nitrofurantoin, and cefazolin, but resistance to amoxicillin, oxacillin, carbenicillin, and tetracycline.Among the resistance genes screened, only <em>aac(6′)-Ib-cr</em> was detected.This study reports the first isolation of C. perfringens type B in Shannan, Tibet; elucidates its biological characteristics and toxin profile; identifies effective antibiotics for targeted intervention; and provides foundational data for controlling lamb dysentery caused by this pathogen and for understanding its pathogenicity in sheep.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100501"},"PeriodicalIF":1.9,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Global distribution and host range of enzootic bovine leukosis in ruminants: A systematic review and meta-analysis 反刍动物地方性牛白血病的全球分布和宿主范围:系统综述和荟萃分析
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-23 DOI: 10.1016/j.vas.2025.100499
Melkie Dagnaw , Getachew Alemu Yilhal , Bemrew Admassu , Yitayew Demessie
{"title":"Global distribution and host range of enzootic bovine leukosis in ruminants: A systematic review and meta-analysis","authors":"Melkie Dagnaw ,&nbsp;Getachew Alemu Yilhal ,&nbsp;Bemrew Admassu ,&nbsp;Yitayew Demessie","doi":"10.1016/j.vas.2025.100499","DOIUrl":"10.1016/j.vas.2025.100499","url":null,"abstract":"<div><div>Enzootic bovine leukosis (EBL) causes severe economic losses and is a contagious disease caused by the Bovine Leukemia Virus (BLV), belonging to the delta retrovirus of the Retroviridae family. Thus, this review aimed to estimate the global pooled prevalence of enzootic bovine leukosis and investigate its host range, focusing on cattle and sheep. We used seven databases which include PubMed, Science Direct, HINARI, Scopus, Google Scholar, Web of Science, and AJOL. The included studies (50 articles), conducted between 1992 and 2024, represent diverse geographic regions: Asia, North America, Africa, South America, and Europe. 346,917 animals were involved, of which 99,620 involved positive for BLV. The meta-analysis estimated a pooled BLV prevalence of 19% (95% CI: 16%–23%) using random effect model, though significant heterogeneity existed across studies (I² = 99.6%, p &lt; 0.001). Subgroup analysis revealed that the highest prevalence of EBL was in North America (43%), followed by Asia (17%), Africa (14%), South America (10%), and the lowest in Europe (4%). Analysis by publication year revealed that the prevalence of EBL was 32% after 2016, whereas it was 29% before 2016. Among animal species, beef cattle exhibited the highest prevalence (26%), followed by overall cattle (20%), sheep (19%), and dairy cattle (18%). Geographical and temporal trends revealed varying prevalence rates, with notable high rates in North America and lower rates in regions such as Europe. It is crucial that North America adopt stricter prevention programs, similar to those in Europe, to reduce transmission of EBL and its economic and health impacts on livestock.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100499"},"PeriodicalIF":1.9,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Camelina straw as an eco-friendly forage alternative for finishing lambs: impacts on performance, rumen function and behavior 亚麻荠秸秆作为生态友好型育肥羔羊饲料替代品:对生产性能、瘤胃功能和行为的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-21 DOI: 10.1016/j.vas.2025.100500
Fatemeh Rezanejad , Seyed Mehdi Ghoreishi , Shahryar Kargar , Mohammad Javad Abarghuei , Ali Kahyani
{"title":"Camelina straw as an eco-friendly forage alternative for finishing lambs: impacts on performance, rumen function and behavior","authors":"Fatemeh Rezanejad ,&nbsp;Seyed Mehdi Ghoreishi ,&nbsp;Shahryar Kargar ,&nbsp;Mohammad Javad Abarghuei ,&nbsp;Ali Kahyani","doi":"10.1016/j.vas.2025.100500","DOIUrl":"10.1016/j.vas.2025.100500","url":null,"abstract":"<div><div>The increasing frequency of droughts and water scarcity has intensified interest in drought-tolerant plants and their by-products as sustainable feed alternatives for ruminants. This study assessed the partial replacement of alfalfa hay (AH) with camelina straw (CS) at 120 (CS12) and 240 (CS24) g/kg of DM in diets of Grey Shirazi lambs (<em>n</em> = 24, average body weight = 22.7 ± 1.7 kg). No significant differences were observed in dry matter intake (DMI), final body weight (FBW: 40.4, 38.8, 40.0 kg), average daily gain (ADG: 285, 260, 278 g/d), or feed conversion ratio (FCR: 5.2, 5.3, 5.1) across treatments. Linear reductions occurred in OM and NDFom digestibility (<em>P</em> &lt; 0.05), with no effects on other nutrients. Ruminal pH, NH<sub>3</sub><sub><img></sub>N, and volatile fatty acids (VFAs) profiles were not significantly altered, except for acetate and isovalerate concentrations, which increased linearly. Partial replacement of AH with CS increased ruminating and total chewing times (<em>P</em> &lt; 0.01), due to higher physically effective fiber intake, without altering meal frequency or duration. These results suggest that camelina straw can be used as a partial AH substitute in lamb finishing diets up to 240 g/kg DM without compromising lamb performance, despite modest reductions in fiber digestibility and increased chewing activity. Future studies should explore behavioral and microbial ecological shifts in the rumen to better understand the mechanisms involved.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"29 ","pages":"Article 100500"},"PeriodicalIF":1.9,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of duplex crystal digital PCR (dPCR) assay for detection and differentiation of NDRV and MDRV 建立NDRV和MDRV双晶数字PCR (dPCR)检测和鉴别方法
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-21 DOI: 10.1016/j.vas.2025.100502
Huixin Liu , Chenchen Wang , Yang He , Qinxuan Wu , Jiayi Chen , Kaijun Wang
{"title":"Development of duplex crystal digital PCR (dPCR) assay for detection and differentiation of NDRV and MDRV","authors":"Huixin Liu ,&nbsp;Chenchen Wang ,&nbsp;Yang He ,&nbsp;Qinxuan Wu ,&nbsp;Jiayi Chen ,&nbsp;Kaijun Wang","doi":"10.1016/j.vas.2025.100502","DOIUrl":"10.1016/j.vas.2025.100502","url":null,"abstract":"<div><div>Muscovy duck reovirus (MDRV) and Novel duck reovirus (NDRV) are highly infectious diseases of waterfowl, causing significant harm to the global poultry industry. Early detection and diagnosis of NDRV and MDRV in clinical samples are crucial for effectively preventing and controlling these diseases. This study developed a duplex crystal digital PCR (dPCR) assay for the differential detection of NDRV and MDRV. Primer pairs and probes were designed specifically for the S3 genome of NDRV and the S2 genome of MDRV. To evaluate the method's performance, different reaction conditions were optimized, focusing on specificity, sensitivity, and reproducibility. The results showed that duplex crystal dPCR could accurately and differentially detect NDRV and MDRV, with a detection limit as low as 1 × 10⁻¹ copies/μl. It did not cross-react with other avian viruses, including duck Tembusu virus (DTMUV), H5 subtype avian influenza virus (H5 subtype AIV), H7 subtype AIV, H9 subtype AIV, Newcastle disease virus (NDV), infectious bronchitis virus (IBV), Egg drop syndrome virus (EDSV), Contagious bursal disease virus (IBDV, B87 strain), and duck Plague virus (DEV). The method also demonstrated good reproducibility, with intra-assay and inter-assay coefficients of variation (CV) both less than 8 %. For clinical application, 299 samples from coastal areas of Guangxi, China were tested. Duplex crystal dPCR detected NDRV and MDRV positive rates of 8.027 % and 6.020 %, respectively, with a co-infection rate of 1.672 %. The kappa values between duplex crystal dPCR and duplex quantitative polymerase chain reaction (qPCR) were 0.977 for NDRV and 1 for MDRV, indicating strong agreement. These findings confirm that the established duplex crystal dPCR is a specific, sensitive, and accurate method for detecting and quantifying NDRV and MDRV. This is the first report of using duplex crystal dPCR for NDRV and MDRV detection.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100502"},"PeriodicalIF":1.9,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of anti-gonadotropin-releasing factor vaccination and administration age on the meat characteristics of pork loins from finishing gilts 抗促性腺激素释放因子接种和给药年龄对肥育后备猪里脊肉质特性的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-19 DOI: 10.1016/j.vas.2025.100498
Ditpon Kotatha , Narut Thanantong , Sukanya Phuengjayaem , Bing-Zheng Li , Alongkot Boonsoongnern , Autchara Kayan , Montri Pattarapanawan
{"title":"Effects of anti-gonadotropin-releasing factor vaccination and administration age on the meat characteristics of pork loins from finishing gilts","authors":"Ditpon Kotatha ,&nbsp;Narut Thanantong ,&nbsp;Sukanya Phuengjayaem ,&nbsp;Bing-Zheng Li ,&nbsp;Alongkot Boonsoongnern ,&nbsp;Autchara Kayan ,&nbsp;Montri Pattarapanawan","doi":"10.1016/j.vas.2025.100498","DOIUrl":"10.1016/j.vas.2025.100498","url":null,"abstract":"<div><div>This pilot study evaluated the effects of anti-gonadotropin-releasing factor (anti-GnRF) vaccination and administration age on gilts (female pigs), with a specific focus on meat characteristics, including nutritional composition, meat quality, and the muscle histology of pork loins. A total of 66 Duroc × (Landrace × Large White) gilts were randomly assigned to three experimental groups: (1) intact gilts; (2) immunized gilts receiving a typical vaccination at 12 and 16 weeks of age; and (3) immunized gilts receiving early vaccination at 9 and 13 weeks of age, which is the earliest schedule recommended by the manufacturer. The pigs were slaughtered at the market age of 24 weeks, with meat samples collected from all of the groups for analysis, including meat samples from intact gilts (MIG), meat from gilts vaccinated at 12 and 16 weeks (MV12/16), and meat from gilts vaccinated at 9 and 13 weeks (MV9/13). Meat from the immunized gilts had significantly higher intramuscular fat (IMF) content than MIG (<em>P</em> &lt; 0.05), accompanied by changes in the fatty acid profile—excluding polyunsaturated fatty acid—without adverse effects on meat quality or muscle histology. No significant differences were observed between the MV12/16 and MV9/13 samples. These findings suggest that anti-GnRF vaccination enhances the IMF content in pork without affecting meat quality or muscle structure. In addition, the administration age did not influence the chemical composition, meat quality, or muscle histology. An early vaccination schedule provides a practical approach to improve gilt production management, facilitating easier pig handling, efficient vaccination administration, and enhanced IMF deposition.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"29 ","pages":"Article 100498"},"PeriodicalIF":1.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chiropractic evaluation in newborn foals: A preliminary study 新生儿马驹的捏脊评估:初步研究
IF 1.9
Veterinary and Animal Science Pub Date : 2025-08-15 DOI: 10.1016/j.vas.2025.100495
Ilaria Arena , Silvia Signor , Jole Mariella , Aliai Lanci , Francesca Freccero , Castagnetti Castagnetti
{"title":"Chiropractic evaluation in newborn foals: A preliminary study","authors":"Ilaria Arena ,&nbsp;Silvia Signor ,&nbsp;Jole Mariella ,&nbsp;Aliai Lanci ,&nbsp;Francesca Freccero ,&nbsp;Castagnetti Castagnetti","doi":"10.1016/j.vas.2025.100495","DOIUrl":"10.1016/j.vas.2025.100495","url":null,"abstract":"<div><div>This paper describes the more frequent chiropractic alterations in healthy and sick foals. The assessment is performed through a motion palpation exam, which locates the hypomobile joints along the skeleton. The motion palpation exam allowed the identification of multiple hypomobile areas in neonatal foals. The sick foals presented a statistically significant higher number of hypomobile joints in the cervical, thoracic, lumbar regions and limbs compared to the healthy foals. The most frequent alterations in sick foals involved the atlantooccipital joint, C5-C6, T14-T15, L5-L6, the sacrococcygeal joint and the scapulohumeral joint. In both groups, high prevalence of alterations occurred at the cervical and sacroiliac joints. The results show that some regions can be highly stressed during the perinatal period, especially in sick foals. Possible explanations are discussed. Chiropractic evaluation and subsequent manipulation could be routinely used to improve neonatal health, especially in sick foals.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100495"},"PeriodicalIF":1.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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