Veterinary and Animal Science最新文献

{"title":"Effects of intra-articular administration of hyaluronic acid or platelet-rich plasma as a complementary treatment to arthroscopy in horses with osteochondritis dissecans","authors":"Marcos F. Pereira ,&nbsp;Gesiane Ribeiro ,&nbsp;Alessandra Gonzales ,&nbsp;Julia A. Arantes ,&nbsp;Renata G.S. Dória","doi":"10.1016/j.vas.2023.100330","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100330","url":null,"abstract":"<div><p>Although arthroscopy is the treatment of choice for horses with osteochondritis dissecans (OCD), it is not yet known whether intra-articular therapies in the postoperative period can bring any benefit to the recovery of these animals. This study evaluated the effects of the intra-articular application of platelet-rich plasma (PRP), hyaluronic acid (HA) or lactated Ringer's solution (LR) in horses with OCD undergoing arthroscopy. Eighteen male and female Brazilian Sport horses aged between 2 and 6 years were evaluated. All animals presented OCD fragments in the middle crest of the tibia. Ten days after surgery, animals were randomly distributed into three groups and received intra-articular application of PRP (<em>n</em> = 6), HA (<em>n</em> = 6), or LR (control group, <em>n</em> = 6). Clinical, radiographic, ultrasound and synovial fluid evaluations were performed on the day of surgery and after 10, 30 and 60 days. An increase in the thickness of the joint capsule was observed 30 days after surgery in the three groups evaluated. In the control group, there was significant improvement in the flexion test 30 and 60 days after surgery, and in the PRP group, there was worsening of this parameter in the same evaluations. In the control group, there was a reduction in the degree of synovial effusion, and in the PRP and HA groups, there was increased effusion. There was a significant increase in the number of leukocytes in the HA group. Intra-articular use of PRP or HA ten days after arthroscopy did not promote positive effects on the recovery of horses with OCD.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"23 ","pages":"Article 100330"},"PeriodicalIF":1.5,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000479/pdfft?md5=6ad912043c4c099aa3a0959730e6aa83&pid=1-s2.0-S2451943X23000479-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different triticale inclusion levels on broilers’ growth parameters: A meta-analysis","authors":"Junior Isaac Celestin Poaty Ditengou ,&nbsp;Sangbuem Cho ,&nbsp;Sung-Il Ahn ,&nbsp;Byungho Chae ,&nbsp;Eunjeong Jeon ,&nbsp;Nag-Jin Choi","doi":"10.1016/j.vas.2023.100328","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100328","url":null,"abstract":"<div><p>Triticale is currently used in poultry nutrition as an alternative energy source to conventional cereals like maize and wheat. Many articles emphasized controversial results regarding its inclusion levels on broiler growth parameters. This study aimed to evaluate the overall impact of triticale and its appropriate incorporation level on the growth performance of broiler chickens via a meta-analysis. In February and April 2022, papers investigating the influence of triticale on broiler's live body weight (LBW), body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were identified on Google Scholar, PubMed, and Science Direct. Based on the eligibility criteria of PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, 19 articles were recorded and divided into 62 experiments according to the inclusion percentages of triticale. Using the random-effects models, Hedges’ g effect size of dietary triticale treatment was calculated to determine the standardized means difference (SMD) at a 95 % confidence interval (95 % CI). The triticale inclusion in the diet increased the broiler's BWG, FI, and FCR but decreased the broiler's LBW. The meta-regression showed that there was a positive correlation between the inclusion of triticale from 0 to 20 % and the broiler's LBW and FI. Although the funnel plot and the Egger linear test suggested significant publication bias for the LBW factor, this study remains valuable for understanding the impact of triticale on broiler growth parameters. In conclusion, the inclusion of triticale up to 20 % in broiler diets could be recommended to obtain optimal performance.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"23 ","pages":"Article 100328"},"PeriodicalIF":1.5,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000455/pdfft?md5=3a3f91eaa80ec5537a2558bbbba1bb62&pid=1-s2.0-S2451943X23000455-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138770142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota alteration with growth performance, histopathological lesions, and immune responses in Salmonella Typhimurium-infected weaned piglets","authors":"Seung-Won Yi ,&nbsp;Han Gyu Lee ,&nbsp;Eunju Kim ,&nbsp;Young-Hun Jung ,&nbsp;Eun-Yeong Bok ,&nbsp;Ara Cho ,&nbsp;Yoon Jung Do ,&nbsp;Kyoung-Min So ,&nbsp;Tai-Young Hur ,&nbsp;Sang-Ik Oh","doi":"10.1016/j.vas.2023.100324","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100324","url":null,"abstract":"<div><p><em>Salmonella</em> Typhimurium can cause gastroenteritis in weaned piglets, which are particularly vulnerable to dietary changes and dysfunction of their immature organs. The colonization of <em>S.</em> Typhimurium could disrupt the gut microbiota and increase susceptibility to the bacterium. This study aimed to investigate the alterations of gut microbiota in <em>S.</em> Typhimurium-infected weaned piglets. Ten 49-day-old pigs were divided into two groups: <em>S.</em> Typhimurium-inoculated (ST, <em>n</em> = 6) and negative control (NC, <em>n</em> = 4) groups. The body weight and <em>S.</em> Typhimurium fecal shedding were monitored for 14 days after <em>S.</em> Typhimurium inoculation (dpi). The intestinal tissues were collected at 14 dpi; histopathological lesions and cytokine gene expression were evaluated. The gut microbiome composition and short-chain fatty acid concentrations were analyzed in fecal samples collected at 14 dpi. The average daily gain and gut microbiota alpha diversity in ST group tended to be lower than NC group at 14 dpi. Linear discriminant analysis effect size results showed a significant increase in the abundance of two genera and five species, while a significant decrease was observed in the five genera and nine species within the gut microbiota of ST group. Among the significantly less abundant bacteria in the ST group, <em>Lachnospira eligens</em> and <em>Anaerobium acetethylicum</em> produce acetate and butyrate, and may be considered as key <em>S.</em> Typhimurium infection-preventing bacteria. The overall results provide invaluable information about changes in the gut microbiota of <em>S.</em> Typhimurium-infected weaned piglets, which can be used to develop alternative measures to antibiotics and prevent ST bacterial infection.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100324"},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000418/pdfft?md5=f6a2cf05e48ac7e02d578917a1848537&pid=1-s2.0-S2451943X23000418-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138474804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of antibiotic, acidifier, and probiotic supplementation on mortality rates, lipoprotein profile, and carcass traits of broiler chickens","authors":"Sabuj Kanti Nath ,&nbsp;Md Taslim Hossain ,&nbsp;Mahfuza Ferdous ,&nbsp;Mst. Assrafi Siddika ,&nbsp;Amir Hossain ,&nbsp;Amim Al Maruf ,&nbsp;Ahanaf Tahmid Chowdhory ,&nbsp;Tilak Chandra Nath","doi":"10.1016/j.vas.2023.100325","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100325","url":null,"abstract":"<div><p>Antimicrobial resistance is a significant issue, therefore it's relevant to assess the effects of antibiotics, acidifiers, and probiotic supplementation finding a good alternative to reduce the use of antibiotics in broiler production in rural areas of Bangladesh. Using randomized control trial, this 28-day study evaluated 360 Hubbard Classic broiler chicks divided into four groups: oxytetracycline-treated, acidifier-treated, Lactobacillus-based probiotic-treated, and control (no antibiotics, acidifiers, or probiotics). Each group was replicated three times with 30 birds each with adlibitum feeding. Body weight and feed intake were recorded weekly, and on 28th day, carcass traits and blood lipoprotein levels were evaluated. Results showed that in first and fourth weeks, the body weight gain significantly varied in probiotics and acidifier-treated birds than the control group (<em>P</em> &lt; 0.001). The probiotic group had gained considerable increase in body weight (185.0 g vs 161.7 g and 1745.0 g vs 1592.7 g) than the control group. Notably, in the first week, the feed conversion ratio for the probiotic group was 0.76, but the antibiotic group's was 0.96 (<em>P</em> &lt; 0.001). The weights of the drumstick (88.33 g) and liver (61.0 g) having probiotic supplements were substantially higher than those in the control group (77.0 g and 51.33 g, respectively) (<em>P</em> &lt; 0.001). According to serum lipoprotein analysis, the probiotic and acidifier groups exhibited lower LDL levels (71.1 mg/dl and 69.8 mg/dl, respectively) and higher triglyceride levels (122.9 mg/dl and 135.4 mg/dl). These findings highlight the potential of probiotics and acidifiers as effective antibiotic alternatives, promoting carcass traits and lowering LDL levels in broilers in Bangladesh.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100325"},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X2300042X/pdfft?md5=a4827e6a388ac9f3e67b699bdd8367c8&pid=1-s2.0-S2451943X2300042X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138472118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of egg yolk-based polyclonal antibodies and immunoprophylactic potential of antigen-antibody complex against infectious bursal disease","authors":"Sanaullah Sajid ,&nbsp;Sajjad ur Rahman ,&nbsp;Mashkoor Mohin ,&nbsp;Zia ud Din Sindhu","doi":"10.1016/j.vas.2023.100326","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100326","url":null,"abstract":"<div><p>A study conducted in the Faisalabad district sampled 50 cases across five IBD outbreaks, revealing an alarming 80 % infection rate among poultry. Our research focused on developing an immune complex (Antigen-antibody complex) with potential immunoprophylactic benefits to counter this formidable threat. Our study was based on producing egg yolk-derived polyclonal antibodies (IgY) targeting IBDV. Commercial layer birds were immunized with inactivated IBDV, yielding IgY antibodies extracted from their eggs exhibited substantially higher and more enduring antibody titers, with a geometric mean titer of 104. Further research involved the creation of an immune complex (ICx) where antigen was extracted from infected bursae tissues. The immunogenic response of ICx was assessed in poultry birds after a challenge with a virulent strain of IBD virus and compared to a conventional IBDV vaccine in poultry. Results revealed significantly higher and more enduring antibody titers induced by the ICx, offering enhanced protective immunity against the IBDV challenge, as evidenced by lower Bursa to bodyweight ratios in vaccinated birds.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"23 ","pages":"Article 100326"},"PeriodicalIF":1.5,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000431/pdfft?md5=a3a80ac8ad4fa5acbb24e68d769f7915&pid=1-s2.0-S2451943X23000431-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138548937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stevia as a natural additive on gut health and cecal microbiota in broilers","authors":"Daniela B. Medeot ,&nbsp;Armando Nilson ,&nbsp;Raul D. Miazzo ,&nbsp;Viviana Grosso ,&nbsp;Walter Ferrari ,&nbsp;Edgardo Jofré ,&nbsp;Arnaldo Soltermann ,&nbsp;María Fernanda Peralta","doi":"10.1016/j.vas.2023.100322","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100322","url":null,"abstract":"<div><p>Stevia mash (SM), leaves of Stevia rebaudiana Bertoni plant, is an additive used in poultry that enhances growth and health. Objective: to determine the effect of 1 % SM on productive parameters, gut health, and the cecal microbiome in broilers between the first 15 and 21 days old. One hundred sixty male, 1-day-old broilers (48.5 ± 2.5 g) were divided into Control (C) without SM and Treated (T) with 1 % SM on diet, during 15/21 days. Each subgroup had eight broilers/five repetitions/treatment. At day 15 or 21, all broilers were dissected, Fabricius Bursa and Gut removed and processed for histomorphometry, followed by Villi Height/Crypt Deep (VH/CD) ratio. Conversion Index (CI) was determined. The V3-V4 region of 16S rRNA gene was amplified from DNA obtained from pooled cecal contents and sequenced on Illumina Miseq PE 2 × 250 platform. Sequence processing and taxonomic assignments were performed using the SHAMAN pipeline. Both T groups have better VH/CD Ratios than C groups (p ≤ 0.05). In guts, increased plasmatic and goblet cells number and thicker mucus layer were found in T15 and T21. All groups received SM showed early immunological maturity in Fabricius Bursa. IC was similar between all treatments. Faecalibacterium, Ruminococcus torques group, and Bacteroides were the major genera modulated by SM addition. At 15 and 21 days old, SM exerts a impact on diversity and evenness of the cecal microbiome.  Conclusion: SM (1 %) produced early immunologic maturity on Fabricius Bursa, increased intestinal functionality, and modified the microbiota, increasing beneficial microbial genera and microbial diversity.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100322"},"PeriodicalIF":1.5,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X2300039X/pdfft?md5=bf1bee6bceb6b3adc05a0df3fbe75f18&pid=1-s2.0-S2451943X2300039X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134832408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of corn silage substitution for dry alfalfa on milk fatty acid profile, nitrogen utilization, plasma biochemical markers, rumen fermentation, and antioxidant capacity in Mahabadi lactating goats","authors":"Shohre Tarverdi Sarabi ,&nbsp;Amir Fattah ,&nbsp;Nader Papi ,&nbsp;Sayyed Roohollah Ebrahimi Mahmoudabad","doi":"10.1016/j.vas.2023.100323","DOIUrl":"10.1016/j.vas.2023.100323","url":null,"abstract":"<div><p>This study aimed to investigate the effects of substituting conventional dry forage with corn silage, a more cost-effective supplementary feed, on a variety of parameters in lactating Mahabadi goats, native to arid and semi-arid regions. A total of fifty lactating Mahabadi goats, aged between 2 and 5 years and weighing an average of 45.3 ± 7.20 kg, were randomly allocated into two experimental groups. The experimental treatments consisted of a control diet and a diet incorporating corn silage at 20 % of dry matter (DM). Various measurements were conducted to assess milk compositions, fatty acid profiles, rumen fermentation parameters, selected plasma biochemical parameters, nitrogen balance, and antioxidant capacity. The results revealed that the substitution of dry forage with corn silage had no statistically significant impact on milk yield, dry matter intake (DMI), body weight, as well as the proportions of monounsaturated and unsaturated fatty acids, and specifically C18:1C9 in milk, non-esterified fatty acid content in milk, rumen concentration of volatile fatty acids (VFA), microbial nitrogen, and nitrogen balance (<em>p</em> &gt; 0.05). Nonetheless, this substitution led to a reduction in the percentage of milk polyunsaturated fatty acids and plasma urea nitrogen content (<em>p</em> &lt; 0.05). It is noteworthy that goats fed with corn silage exhibited a notably higher antioxidant capacity in rumen liquor (<em>p</em> &lt; 0.05), plasma, and milk (<em>p</em> &lt; 0.01) compared to those receiving the control diet. The substitution of conventional dry forage with corn silage exhibited no adverse effects on the performance of lactating Mahabadi goats. On the contrary, it contributed to an enhancement in the antioxidant status. These findings offer valuable insights for optimizing dietary composition by incorporating corn silage at 20 % of DM, thereby improving milk quality, reducing costs, and promoting overall health in lactating goats, particularly within arid and semi-arid regions.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100323"},"PeriodicalIF":1.5,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000406/pdfft?md5=4554009a42c454e937cfe7181c630c09&pid=1-s2.0-S2451943X23000406-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological changes in broiler chickens subjected to dietary ajwain (trachyspermum ammi l.) essential oil in encapsulated and conventional forms within a wheat-based diet","authors":"Shokoufe Ghazanfari ,&nbsp;Cyrus Akbari Shadbad ,&nbsp;Amir Meimandipor ,&nbsp;Seyed Abdollah Hosseini ,&nbsp;Shirin Honarbakhsh","doi":"10.1016/j.vas.2023.100321","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100321","url":null,"abstract":"<div><p>The aim of this study was to investigate the effects of different diet types, forms, and contents of ajwain essential oil (AEO) on various physiological characteristics of broiler chickens, including cell-mediated immunity responses, intestinal morphology, and microflora. A total of 1500 one-day-old male broiler chickens were allocated to different treatments based on a 2 × 3 × 2 factorial arrangement, considering diet types (corn and corn-wheat), contents of AEO (0, 150, and 300 mg/kg of diet), and forms of AEO (conventional and encapsulated). The results indicated that the broiler chickens fed the diet containing 150 ppm EO demonstrated reduced skin thickness in response to a 2,4-dinitrochlorobenzene challenge, 24 h after injection, compared to those receiving a diet without EO (<em>P</em> &lt; 0.05). Increasing the EO content led to an increase in the villous height to crypt depth ratio in the jejunum of broiler chickens receiving 300 ppm EO (<em>P</em> &lt; 0.05). Moreover, there was a slight improvement in the villous height to crypt depth ratio in the jejunum of broiler chickens fed the corn-wheat diet (<em>P</em> = 0.07). Broiler chickens fed the 300 ppm EO showed a lower total bacterial population compared to those fed the 150 ppm EO (<em>P</em> &lt; 0.05). Finally, the use of EO at a content of 150 ppm improved cellular immune response, while EO at a content of 300 ppm improved the morphology and overall population of intestinal bacteria. Furthermore, the inclusion of wheat-corn diets exhibited enhanced morphological characteristics of the intestines. However, the forms of AEO did not exert any significant influence on the physiological traits.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100321"},"PeriodicalIF":1.5,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000388/pdfft?md5=66ae12f636aef8e63b8261b3cf598d63&pid=1-s2.0-S2451943X23000388-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dried cabbage (Brassica oleracea var. capitata) waste meal decreases blood cholesterol but does not alter growth performance, and physiological indices of weaned pigs","authors":"Makinde O. John ,&nbsp;Olaiya O. David ,&nbsp;Egbo L. Mary ,&nbsp;Kalla D. Joab ,&nbsp;Adelowo V. Olayinka ,&nbsp;Akeem B. Sikiru ,&nbsp;Bossima I. Koura","doi":"10.1016/j.vas.2023.100320","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100320","url":null,"abstract":"<div><p>The study investigated the potentials of dried cabbage waste meal (DCWM) on growth performance, blood biochemical characteristics, physiological indices, and economics of production of weaned pigs. Ninety crossbred (Large white X Landrace; 8.01 ± 0.18 kg body weight) clinically certified weaned pigs of equal sexes were randomly allocated to five dietary treatments containing DCWM. Each treatment consisted of 18 pigs replicated thrice in a completely randomized design. Diet 1 (control diet) contained no DCWM whereas diets 2, 3, 4 and 5 had 50, 100, 150 and 200 g/kg levels of inclusion of DCWM, respectively, replacing parts of the feed. The study lasted for 8-weeks. Feed and water were available <em>ad-libitum.</em> Average daily feed intake (715.76–780.03 g/d) increased (<em>p</em> &lt; 0.05) while average daily weight (294.17–301.74 g/d) and feed conversion ratio (2.39–2.65) were similar (<em>p</em> &gt; 0.05). White blood cell (12.11–14.62 × 10⁹/L), lymphocytes (62.93–70.30%), Uric acid (7.74–9.99 mmol/L), HDL (0.89–1.27 mmol/L) and K (130.90–145.72 mmol/L) concentrations increased (<em>p</em> &lt; 0.05) whereas cholesterol (2.30–5.06 mmol/L) and triglyceride (0.33–0.87 mmol/L) concentrations decreased (<em>p</em> &lt; 0.05) in response to increased DCWM in the diets. Total cost of feeding ($17.96–20.89) reduced (<em>p</em> &lt; 0.05) while gross benefits ($48.97–52.30) were significantly better (<em>p</em> &lt; 0.05) for pigs fed 100, 150 and 200 g/kg DCWM compared to those fed 0 and 50 g/kg diets. DCWM in pig diets up to 200 g/kg had positive influence on blood cholesterol and economics of production.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100320"},"PeriodicalIF":1.5,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000376/pdfft?md5=d60412ba91f78fe84397a491076b66e4&pid=1-s2.0-S2451943X23000376-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: Amniotic fluid-derived mesenchymal stem cell treatment in a dog with a spinal cord injury","authors":"Eun Young Kim ,&nbsp;Tae Young Kil ,&nbsp;Min Kyu Kim","doi":"10.1016/j.vas.2023.100318","DOIUrl":"https://doi.org/10.1016/j.vas.2023.100318","url":null,"abstract":"<div><p>Spinal Cord Injury (SCI) refers to complete or incomplete damage to the spinal cord, which comprises the central nervous system. SCI in dogs, like humans, is mostly caused by external trauma, and the degree of impact is dependent on the location of the injury in the spine. Stem cell therapy is a promising avenue for SCI research. In this report, we investigate the therapeutic potential of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) in dogs with spinal cord injuries. A 2-year-old male beagle dog presented with sensory and motor incomplete symptoms resulting in an inability to control the legs, hips, and genitourinary system due to an injury in the lumbar region of the spinal cord. In addition to the administration of surgical decompression, AF-MSCs were directly injected into the damaged spinal tissue. Approximately 15–16 weeks after stem cell transplantation, the dog's hind limb movement improved, and spinal cord regeneration was confirmed through magnetic resonance imaging (MRI). Eventually, the dog was able to walk independently, although not perfectly. In conclusion, AF-MSC-based stem cell transplantation may be beneficial for SCIs.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"22 ","pages":"Article 100318"},"PeriodicalIF":1.5,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X23000352/pdfft?md5=fd519dd6087807692927a9fdd547a6cb&pid=1-s2.0-S2451943X23000352-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}