阉割法对猪骨骼肌纤维特征和转录组特征的影响

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Klavdija Poklukar , Anja Erbežnik , Gregor Fazarinc , Kevin Kress , Nina Batorek-Lukač , Martin Škrlep , Volker Stefanski , Marjeta Čandek-Potokar , Milka Vrecl
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引用次数: 0

摘要

本研究考察了免疫阉割和手术阉割对猪骨骼肌组织形态计量和转录组特征的影响。我们假设,不同阉割方法导致的雄激素剥夺持续时间的差异会以肌肉特异性的方式影响骨骼肌生物学。我们使用酶/免疫组化分类和肌纤维组织形态分析、定量 PCR 和 RNA 测序方法,分析了免疫阉割猪(IC;n = 12)、全雄猪(EM;n = 12)和手术阉割猪(SC;n = 12)的背阔肌(LD)和半月板头肌(SSC)样本,从而验证了这一假设。结果证实,LD 和 SSC 的组织形态学特征各不相同,阉割方法在组织形态学和转录组水平上具有肌肉特异性效应。长期雄激素缺乏(手术阉割)会明显降低 LD 快速肌动 IIa 型肌纤维的比例(P < 0.05),而短期雄激素剥夺(免疫阉割)会减少 SSC 氧化 I 型肌纤维的横截面积(P < 0.05)。在转录水平上,糖酵解 LD 通过上调控制肌细胞增殖和分化的基因来适应长期和短期的雄激素剥夺,以维持纤维的大小。相反,通过泛素连接酶介导的萎缩途径(TRIM63和FBXO32表达显著增加;P <0.05)增加的蛋白质降解可能是IC中氧化性SSC的I型肌纤维横截面积减少的原因。在 IC 中还发现了与特定代谢特征和肉质性状相关的潜在候选基因(HK2、ARID5B、SERPINE1 和 SCD),为研究免疫排斥对骨骼肌纤维和胴体/肉质性状的影响奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of castration method on porcine skeletal muscle fiber traits and transcriptome profiles

This study examined the effects of immunocastration and surgical castration on the histomorphometric and transcriptome traits of the porcine skeletal muscle. We hypothesized that the differences in duration of androgen deprivation resulting from different castration methods influence skeletal muscle biology in a muscle-specific manner. This was tested by analyzing samples of m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) from immunocastrated (IC; n = 12), entire male (EM; n = 12), and surgically castrated (SC; n = 12) pigs using enzyme/immunohistochemical classification and histomorphometric analysis of myofibers, quantitative PCR, and RNA sequencing. The results confirmed the distinctive histomorphometric profiles of LD and SSC and the castration method related muscle-specific effects at the histomorphometric and transcriptome levels. Long-term androgen deficiency (surgical castration) significantly reduced the proportion of fast-twitch type IIa myofibers in LD (P < 0.05), whereas short-term androgen deprivation (immunocastration) reduced the cross-sectional area of oxidative type I myofibers in SSC (P < 0.05). At the transcriptional level, glycolytic LD adapted to long- and short-term androgen deprivation by upregulating genes controlling myoblast proliferation and differentiation to maintain fiber size. In contrast, increased protein degradation through the ubiquitin ligase-mediated atrophy pathway (significantly increased TRIM63 and FBXO32 expression; P < 0.05) could underly reduced cross-sectional area of type I myofibers in the oxidative SSC in IC. Potential candidate genes (HK2, ARID5B, SERPINE1, and SCD) linked to specific metabolic profiles and meat quality traits were also identified in IC, providing a foundation for studying the effects of immunocastration on skeletal muscle fiber and carcass/meat quality traits.

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来源期刊
Veterinary and Animal Science
Veterinary and Animal Science Veterinary-Veterinary (all)
CiteScore
3.50
自引率
0.00%
发文量
43
审稿时长
47 days
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