Mehri Montazerharzand , Hamid Paya , Akbar Taghizadeh , Ali Hosseinkhani , Mohammad Ramin
{"title":"Effects of walnut shell biochar feed additive on rumen fermentation, nutrient utilization, and performance in fattening lambs","authors":"Mehri Montazerharzand , Hamid Paya , Akbar Taghizadeh , Ali Hosseinkhani , Mohammad Ramin","doi":"10.1016/j.vas.2025.100515","DOIUrl":"10.1016/j.vas.2025.100515","url":null,"abstract":"<div><div>Converting agricultural waste into biochar offers a promising approach to improve animal nutrition and mitigate environmental impacts. This study investigated the effects of dietary supplementation with walnut shell-derived biochar on growth performance, nutrient digestibility, and rumen fermentation characteristics in fattening male lambs. Twelve lambs (initial body weight: 34.4 kg) were randomly assigned to two dietary treatments: a control diet and a diet supplemented with 1 % walnut shell biochar, over a period of 60 days. The inclusion of 1 % walnut shell biochar did not significantly affect dry matter intake (DMI) (<em>p</em> = 0.08), average daily gain (ADG) (<em>p</em> = 0.06), or feed conversion ratio (FCR) (<em>p</em> = 0.47). However, lambs fed the walnut shell biochar diet had a higher final body weight compared to the control group (<em>p</em> = 0.05). In contrast, lambs fed the walnut shell biochar -supplemented diet showed significantly higher digestibility coefficients of organic matter (OM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) (<em>p</em> < 0.05). Rumen fermentation parameters were also influenced by the walnut shell biochar supplement, with increased total volatile fatty acid (VFA) concentration (<em>p</em> = 0.03), higher propionate levels (<em>p</em> = 0.054), and reduced protozoa counts (<em>p</em> = 0.0003), while rumen pH (<em>p</em> = 0.76) and ammonia nitrogen (N<img>NH3) concentrations (<em>p</em> = 0.64) remained unaffected. These findings suggest that walnut shell biochar may improve fiber and organic matter digestibility and beneficially modulate rumen fermentation without compromising growth performance in lambs.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100515"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chandler Higgs , Megan Van Noord , Jefferson Gabriel Carvalho Nagle , Jose Pablo Gomez , Erik Fausak , Gabriele Ute Maier
{"title":"Dam vaccination for the prevention of neonatal diarrhea caused by enterotoxigenic E. coli in calves-a systematic review and meta-analysis","authors":"Chandler Higgs , Megan Van Noord , Jefferson Gabriel Carvalho Nagle , Jose Pablo Gomez , Erik Fausak , Gabriele Ute Maier","doi":"10.1016/j.vas.2025.100546","DOIUrl":"10.1016/j.vas.2025.100546","url":null,"abstract":"<div><div>Diarrhea in neonatal calves caused by enterotoxigenic <em>Escherichia coli</em> (ETEC) is a disease that negatively affects the welfare and production of cattle. We present a review of all literature published in 1950 and later, evaluating vaccine effectiveness and efficacy in protecting calves from diarrhea and death from infection with ETEC by vaccinating dams during gestation. Of 3677 citations identified, 61 were eligible for full text review. Data was extracted from 22 citations and separated into 3 subgroups: field trials, heterologous challenge trials, and homologous challenge trials. Field trials showed no evidence to support that vaccinating dams decreased the rate of death or diarrhea in calves under 14 days of age, but due to a limited number and quality of field trials that met our inclusion criteria, it is difficult to draw general conclusions from this finding. However, in both homologous and heterologous challenge trials, vaccines helped prevent death in study calves. The outcome diarrhea was eliminated from analysis in challenge trials due to unexplained statistical heterogeneity which may be caused by a variety of definitions for diarrhea. Very few studies included information on randomization, blinding, or funding sources. Based on funnel plots, some indication for publication bias exists for heterologous challenge trials, however, a sensitivity analysis using the trim and fill method did not change results. There is evidence for the efficacy of ETEC vaccination of dams in the prevention of neonatal calf death.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100546"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145579049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nutrients utilization and enteric methane emission in zebu cattle fed low quality forages","authors":"Gérard Xavier Gbenou , Luc Hippolyte Dossa , Denis Bastianelli , Ollo Sib , Laurent Bonnal , Cécile Martin , Mohamed Habibou Assouma","doi":"10.1016/j.vas.2025.100511","DOIUrl":"10.1016/j.vas.2025.100511","url":null,"abstract":"<div><div>During the dry season in sub-Saharan Africa, ruminants are commonly fed low-quality forages, such as rangeland fodder and harvested grasses. This study evaluated the effects of four such forages - rangeland fodder (RF), <em>Brachiaria ruziziensis</em> (Bruzi), <em>Andropogon gayanus</em> (Agaya), and <em>Panicum maximum</em> (Pmaxi), all harvested at maturity (24 months) on feed intake, diet digestibility, and enteric methane (eCH₄) emissions. Ten Sudanese Fulani zebu steers (27.7 ± 4.6 months of age, 143.7 ± 15.7 kg of initial body weight - BW) were fed each forage at 2.3 % and 3.2 % of body weight (dry matter – DM basis) over three-week periods, two weeks of adaptation and one week of data collection. The forages differed significantly in chemical composition, affecting feed intake, diet digestibility, and eCH₄ emissions. The Pmaxi, with superior nutritional quality, was the most consumed and had the highest apparent digestibility (0.49; <em>P</em> < 0.001). At low-quality stage, all the forages had similar eCH<sub>4</sub> yield potential (28.5 - 31.6 g/kg DM intake; <em>P</em> = 0.486). Methane conversion rate (Ym) ranged from 8.6 % in Pmaxi to 9.6 % of gross energy intake in Agaya; however, the variation was not statistically significant. Feeding ruminants over ad libitum low-quality forages did not affect their feed intake, diet apparent digestibility and eCH<sub>4</sub> emissions. Measuring eCH<sub>4</sub> emissions induced by these forages at different growth stages can help identify their optimal use for promoting both feed and environmental sustainability.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100511"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phenotypic characterization of indigenous Xhosa goat ecotype in three agro-ecological zones in the eastern cape province, South Africa","authors":"Sibulele Praise Ntonga , Oluwakamisi Festus Akinmoladun , Ziyanda Mpetile","doi":"10.1016/j.vas.2025.100512","DOIUrl":"10.1016/j.vas.2025.100512","url":null,"abstract":"<div><div>This study characterized the phenotypic attributes of indigenous Xhosa goats across three agro-ecological zones and developed predictive models for estimating body weight. A total of 450 Xhosa goats were sampled using a stratified random approach based on sex, age, and agro-ecological region. Morphometric traits, including body weight (BW), heart girth (HG), body length (BL), wither height (WH), and body depth (RD), were recorded. Data were analysed using the General Linear Model (GLM) of SPSS (v.20) to assess the effects of zones, sex, and age on body traits, while Pearson’s correlation and stepwise regression identified the best predictors of body weight. Results showed significant (p < 0.05) variations in body measurements across zones, with Savanna goats exhibiting superior traits. Males had significantly (p < 0.05) higher BW and body dimensions than females and castrates. Correlation analysis revealed HG (r=0.80), BL (r=0.84), and RD (r=0.82) as the strongest predictors of BW in males, while BL (r=0.66), HG (r=0.65), WH (r=0.62), and RD (r=0.61) were best for females. Stepwise regression identified HG and BL as the best predictors for males, and HG, BL, and SH for females. The predictive models (R² = 0.74–0.85) provide a practical tool for estimating body weight in field conditions. These findings provide practical tools for field-based weight estimation and highlight the importance of conserving the phenotypic diversity of Xhosa goats for sustainable breeding and genetic improvement programs in South Africa.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100512"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hardi Ahmed Karim AL-JAF , Saman Abdulmajid Rashid
{"title":"Synergistic effects of zinc oxide and selenium nanoparticles in drinking water on broiler chicken growth, antioxidant, and immunity responses","authors":"Hardi Ahmed Karim AL-JAF , Saman Abdulmajid Rashid","doi":"10.1016/j.vas.2025.100528","DOIUrl":"10.1016/j.vas.2025.100528","url":null,"abstract":"<div><div>The poultry sector faces several nutritional and health challenges, including deficiencies in essential micronutrients such as zinc and selenium, which, although required in low levels, are important for growth and immune function. Nanotechnology has emerged as a promising tool to enhance nutrient bioavailability and efficacy. While individual benefits of Zn and Se nanoparticles (NPs) have been demonstrated, research on their combined effects in broilers is limited. In this study, we aimed to evaluate the effects of combined zinc oxide (ZnO) and Se NPs in drinking water on the performance, gut morphology, antioxidant capacity, and humoral immunity of broiler chickens. A total of 252 one-day-old chicks were randomly assigned for 35 days to six treatment groups, each consisting of six replicates with seven birds per experimental cage. The treatments included a negative control (T1) without Zn and Se supplementation, a positive control (T2) with inorganic Zn and Se supplementation, and four treatments (T3, T4, T5, and T6) receiving combined ZnO and Se NPs at doses of 35 and 0.15, 70 and 0.3, 35 and 0.3, and 70 and 0.15 mg/L, respectively. Birds receiving combinations of ZnO and Se NPs demonstrated significant improvements in body weight, feed efficiency, and feed intake. Enhanced villus height in the small intestine, increased antioxidant enzyme activities, and reduced malondialdehyde levels were also observed. Antibody titres against Newcastle disease virus were significantly elevated. In conclusion, combined ZnO and Se NPs can enhance growth, physiological status, and immune function in broiler chickens.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100528"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alessandro Guerrini , Luisa Vera Muscatello , Francesca del Zozzo , Valeria Harper , Lorenzo Fiorini , Ivonne Laura Archetti , Valentina Serra , Claire Carlu , Thibaut Chabrillat , Doriana Eurosia Angela Tedesco
{"title":"Hepatoprotective effects of silibinin and chlorogenic acid standardised extract to alleviate the fatty liver syndrome and improve blood indices in caged-laying hens after the peak of production","authors":"Alessandro Guerrini , Luisa Vera Muscatello , Francesca del Zozzo , Valeria Harper , Lorenzo Fiorini , Ivonne Laura Archetti , Valentina Serra , Claire Carlu , Thibaut Chabrillat , Doriana Eurosia Angela Tedesco","doi":"10.1016/j.vas.2025.100522","DOIUrl":"10.1016/j.vas.2025.100522","url":null,"abstract":"<div><div>This study was designed to assess the effectiveness of a mixture of <em>Silybum marianum</em> L. and <em>Cynara cardunculus scolymus</em> L. standardised extract (PHYTO-LAYER™) expressed as silibinin (2.4 g/L) and chlorogenic acid (2.2 g/L) content, respectively, provided intermittently to caged-laying hens to alleviate fatty liver syndromes, improve blood indices and health. In this study, 792 Lohmann LSL-White hens (41 weeks of age), randomly assigned to 2 replicated groups, control and treated (<em>n</em> = 396 each), were used. The treatment (1 mL/L) was supplied every 2 weeks for 7 days, at different time points (T0-T5) in a 7-week trial, by drinking water. At T0, T3 and T5, 13 identified hens/group were sampled for haematological analysis. At T5, all 13 hens per group were euthanised via cervical dislocation for organ weight, gut pH, and liver histological investigations. The treatment improved red blood cell, haemoglobin, hematocrit, and the serum antioxidant activity (<em>P</em> < 0.05). In treated hens, a reduction of liver weight and serum alanine aminotransferase activity was evidenced, associated with a reduction of liver steatosis cases and severity, evaluated histologically (<em>P <</em> 0.05). Other blood and serum indices were not affected by the treatment (<em>P</em> > 0.05). At T5, in treated hens, the duodenum (proximal tract) showed unexpectedly higher pH, compared to the control hens (<em>P <</em> 0.05). This study showed that the combined phytoextracts administration is useful in improving the liver health of caged-laying hens affected by liver disorders, reducing liver steatosis, associated with an upgrade of main blood indices.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100522"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md. Abul Bashar , Mst. Mahomudha Akhtar , Shahanaj Ferdousi Shejuty , Gautam Kumar Deb , Sadek Ahmed , M. A. M. Yahia Khandoker
{"title":"Effect of glutathione, ascorbic acid, and their combination on the quality and fertility of cryopreserved spermatozoa of Black Bengal buck","authors":"Md. Abul Bashar , Mst. Mahomudha Akhtar , Shahanaj Ferdousi Shejuty , Gautam Kumar Deb , Sadek Ahmed , M. A. M. Yahia Khandoker","doi":"10.1016/j.vas.2025.100549","DOIUrl":"10.1016/j.vas.2025.100549","url":null,"abstract":"<div><div>This study investigated the effect of the addition of reduced glutathione (GSH), <span>l</span>-ascorbic acid (AA), and their combination (GSH+AA) to the semen extender on the semen quality and fertility of post-thaw spermatozoa of Black Bengal bucks. Ejaculates (<em>n</em> = 30) collected from five Black Bengal bucks were evaluated, pooled (37 °C), and divided into four treatment groups: a control group (no antioxidants), a GSH group (control + 1 mM GSH), an AA group (control + 8 mM AA), and a combined group (control + 1 mM GSH and 8 mM AA). The semen samples were then filled into 0.25 mL French straws and cryopreserved following a standard cryopreservation method. Supplementation of GSH (1 mM) and AA (8 mM) significantly (<em>P</em> < 0.05) improved total motility, progressive motility, normal morphology (normal fraction, distal droplet, and bent tail), and kinematic parameters (average path velocity, straight-line velocity, curvilinear velocity, and beat cross frequency) of the spermatozoa. However, the maximum improvement was observed with the combined group. In terms of malondialdehyde (MDA) levels and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) status, the values of MDA and H<sub>2</sub>O<sub>2</sub> were reduced significantly (<em>P</em> < 0.05) in the GSH, AA, and GSH+AA groups compared to the control group. The fertilization capacity of the cryopreserved spermatozoa was significantly higher (<em>P</em> < 0.05) in the antioxidant-treated groups compared to the control group. In conclusion, supplementation of GSH and AA in freezing medium has a synergistic and beneficial effect on the quality and fertility of cryopreserved spermatozoa of Black Bengal buck.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100549"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145624095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bernau , T. Schilling , H. Eßlinger , L.E. Hoelzle , S.A. Goth
{"title":"Infrared thermographic imaging as a promising on-farm method to estimate parasite load in dairy goats","authors":"M. Bernau , T. Schilling , H. Eßlinger , L.E. Hoelzle , S.A. Goth","doi":"10.1016/j.vas.2025.100506","DOIUrl":"10.1016/j.vas.2025.100506","url":null,"abstract":"<div><div>Faecal egg count (FEC) evaluation is the method of choice to detect parasite load in individuals, but it cannot be used directly on farm. Clinical parameters like body condition scores (BCS), body weight (BW) and milk yield (MY) have been described in this context, but all fail due to a low correlation with FEC. The present study evaluated the utility of clinical parameters, linear body measurements, and infrared thermographic imaging (IRT) in eight dairy goat herds in Germany as potential methods for estimating FEC categories. A total of 893 dairy goats comprising German Fawn Goats (GFG) and German White Goats (GWG) were examined up to six times during three consecutive lactation periods. FEC was determined using individual faecal samples, analysed via the McMaster method. Based on eggs per gram of faeces (EPG), mean FEC across all examinations was categorized into three groups: low (Category 1: ≤500 EPG), medium (Category 2: >500 to ≤1500 EPG), and high (Category 3: >1500 EPG). Statistical analyses were conducted using SAS Studio to identify factors influencing FEC. Significant differences were observed between the two breeds.</div><div>In GFG, animals in Category 3 exhibited significantly lower BCS, BW, chest circumference, and MY compared to those in Category 1. IRT measurements also showed significant differences across FEC categories, with higher IRT values observed in goats with higher FEC. These findings suggest that IRT has potential as a non-invasive, on-farm method for assessing parasitic load. However, further studies are necessary to validate its practical application as a direct health monitoring method.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100506"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sen Zhang , Liyuan Song , Xin Yin , Changmin Hu , Yingyu Chen , Aizhen Guo
{"title":"Robust BICP0-gB indirect ELISA for the accurate diagnosis of bovine alphaherpesvirus 1 infections","authors":"Sen Zhang , Liyuan Song , Xin Yin , Changmin Hu , Yingyu Chen , Aizhen Guo","doi":"10.1016/j.vas.2025.100507","DOIUrl":"10.1016/j.vas.2025.100507","url":null,"abstract":"<div><div>Bovine alphaherpesvirus 1 (BoHV-1) represents a significant threat to the cattle industry, emphasizing the need for reliable diagnostic tools that facilitate effective disease management. Current diagnostic methods, including virus neutralization tests (VNTs), are often complex and labor intensive, but existing antibody detection assays may lack sufficient accuracy. In this study, we developed a novel indirect enzyme-linked immunosorbent assay (iELISA) utilizing both BICP0 and gB proteins to enhance the detection of BoHV-1 infections. The inclusion of BICP0, a pivotal protein during the early stages of viral infection, markedly improved the assay's specificity and sensitivity. The BICP0-gB iELISA exhibited a high degree of concordance with the VNT, demonstrating superior sensitivity and specificity. Preliminary clinical evaluations revealed a real prevalence of 40.3 % (95 % CI: 33.1 %-48.0 %) in serum samples from yaks in Qinghai Province, which aligns with the documented prevalence of BoHV-1 in the region. These results underscore that BICP0-gB iELISA is a robust and reliable diagnostic tool for the differential diagnosis of BoHV-1, providing a cost-effective and efficient solution for high-throughput screening in the livestock industry.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100507"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}