Veterinary and Animal Science最新文献

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Assessment of maternal-offspring selenium transfer in ewes and newborn lambs via serum, whole blood, and wool matrices 通过血清、全血和羊毛基质评估母儿硒在母羊和新生羔羊中的转移
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-24 DOI: 10.1016/j.vas.2025.100527
Mehmet Çalışkan
{"title":"Assessment of maternal-offspring selenium transfer in ewes and newborn lambs via serum, whole blood, and wool matrices","authors":"Mehmet Çalışkan","doi":"10.1016/j.vas.2025.100527","DOIUrl":"10.1016/j.vas.2025.100527","url":null,"abstract":"<div><div>Selenium (Se) is an essential micronutrient required by almost all organisms. Disorders associated with Se deficiency can occur in various animal species, but they are more common in ruminants, especially small ruminants. This study had two main objectives: first, to investigate whether there is a correlation between whole blood, serum, and wool Se levels in sheep and lambs individually; and second, to examine the relationship between Se levels in these matrices between ewes and their newborn lambs. Wool samples were collected from the sulcus jugularis region using a razor blade, and blood samples were obtained via jugular venipuncture from 18 ewes and their 18 colostrum-fed lambs aged 12 to 72 h. Significant correlations were found between serum and whole blood Se levels within both ewes (<em>r</em> = 0.72, <em>p</em> &lt; 0.01) and lambs (<em>r</em> = 0.69, <em>p</em> &lt; 0.01), indicating internal consistency within each group. Se concentrations in whole blood, serum, and wool samples were similar between ewes and lambs, with no statistically significant differences observed in any matrix (<em>p</em> &gt; 0.05). While Se concentrations in whole blood showed a significant correlation between ewes and lambs (<em>r</em> = 0.65, <em>p</em> &lt; 0.01), no such correlation was observed in serum or wool samples. These findings suggest that both whole blood and serum are suitable for assessing individual Se status in sheep and lambs, whereas whole blood may serve as a more reliable matrix for evaluating maternal-offspring Se transfer.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100527"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Feline lymphoma associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) infections in Brazil- Systematic review 巴西与猫白血病病毒(FeLV)和猫免疫缺陷病毒(FIV)感染相关的猫淋巴瘤——系统综述
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.vas.2025.100514
Pedro Antônio Bronhara Pimentel , Antonio Giuliano , Fernanda Vieira Amorim da Costa , Paweł Marek Bęczkowski , Letícia Neves Ribeiro , Breno Neves Manzalli Oliveira , Raquel Fernandes Pereira , Rodrigo dos Santos Horta
{"title":"Feline lymphoma associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) infections in Brazil- Systematic review","authors":"Pedro Antônio Bronhara Pimentel ,&nbsp;Antonio Giuliano ,&nbsp;Fernanda Vieira Amorim da Costa ,&nbsp;Paweł Marek Bęczkowski ,&nbsp;Letícia Neves Ribeiro ,&nbsp;Breno Neves Manzalli Oliveira ,&nbsp;Raquel Fernandes Pereira ,&nbsp;Rodrigo dos Santos Horta","doi":"10.1016/j.vas.2025.100514","DOIUrl":"10.1016/j.vas.2025.100514","url":null,"abstract":"<div><div>Lymphoma is the most common neoplasia in cats and is frequently linked with concurrent retroviral infections. Despite preventive strategies, feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) remain endemic in many parts of the world, with FeLV significantly shortening the lifespan of infected cats. In Brazil and other low-income countries, the incidence of FeLV and FIV remains high. However, the distribution of these retroviruses across South America is still unclear, and their correlation with the anatomical and clinical features of feline lymphoma remains poorly understood. Gaining a deeper understanding of these associations could lead to more effective disease control strategies and better-defined chemotherapy protocols. In this study, we examined the geographical distribution and clinical presentations of feline lymphoma in retrovirus-positive cats in Brazil. A modified bias risk analysis assessed the quality of published studies, and a map was created to highlight areas in need of further research. Initial screening of 703 studies identified 29 relevant ones, encompassing 607 cats with feline lymphoma. After bias analysis, 273 cases were included in this review: 190 were FeLV-positive and FIV-negative, 98 were FeLV-negative and FIV-negative, and 15 were FeLV-negative and FIV-positive. Cases were reported from six Brazilian states and the Federal District of Brazil (2000–2023). The anatomical location of lymphoma varied depending on the cats' viral status, with notable differences in intestinal and mediastinal manifestations. FeLV-negative cats showed an 11.1-fold increased risk (95 % CI=6.0–20.0) for intestinal lymphoma, while FeLV-positive cases had a 7.5-fold increased risk (95 % CI=3.7–15.6) for mediastinal lymphoma. Due to the scarcity of FIV-related cases, analyzing associations was not feasible. Given the apparent association between FeLV-positive status and lymphoma location, further research into this neoplasm is strongly encouraged. We also recommend widespread retrovirus testing and expanded FeLV vaccination efforts in Brazil.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100514"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modeling growth performance of heterogeneous rabbits in a pastured system using nonlinear, spline and random regression models 利用非线性、样条和随机回归模型模拟放牧系统中异种兔的生长性能
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1016/j.vas.2025.100540
Hameed Akande Bashiru, Saidu Oyarekhua Oseni
{"title":"Modeling growth performance of heterogeneous rabbits in a pastured system using nonlinear, spline and random regression models","authors":"Hameed Akande Bashiru,&nbsp;Saidu Oyarekhua Oseni","doi":"10.1016/j.vas.2025.100540","DOIUrl":"10.1016/j.vas.2025.100540","url":null,"abstract":"<div><div>Accurate modeling of growth trajectories is critical for decision-making in rabbit production, particularly under low-input systems. However, limited studies have evaluated the comparative performance of growth models for rabbits reared in such systems. This study evaluated the growth performance of heterogeneous rabbits using three modeling approaches. Weekly body weight records of 343 rabbits from birth to 20 weeks of age across four parities were analyzed. Four classical nonlinear models (Gompertz, Logistic, von Bertalanffy, and Richards) were fitted using the NLIN procedure of SAS®. Further, the REG procedure of SAS® was used to fit four linear spline models (SP3 to SP6) while four random regression models with varying variance-covariance structures (Compound Symmetry, Unstructured, Autoregressive, and Toeplitz) were also fitted to the growth data using the MIXED procedure of SAS®. There were significant effects of parity (<em>p</em> &lt; 0.05) on growth, with kits from later parities consistently achieving higher body weights than those from earlier parities. Among nonlinear models evaluated, the von Bertalanffy model had the lowest AIC, ΔAIC, Akaike weight and BIC values and was therefore selected as the best fit model. However, all spline regression models performed poorly and consistently over-estimated body weight at all ages. Random regression analysis also showed that the Unstructured model had the best performance in terms of goodness-of-fit tests evaluated. The findings suggest that nonlinear models especially the von Bertalanffy and random regression models with flexible covariance structures provide more accurate and biologically interpretable fits than spline models for growth prediction in heterogeneous rabbits.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100540"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145579047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A bromoform based Investigational Veterinary Product fed twice daily to lactating dairy cows had no adverse effects on eating, rumination, or locomotion behaviours 一种基于溴仿的研究性兽医产品,每天两次喂给泌乳奶牛,对进食、反刍或运动行为没有不良影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-11-10 DOI: 10.1016/j.vas.2025.100539
R. Tognelli , P.S. Alvarez-Hess , A.S. ó Neachtain , S. Chandra , S.R.O. Williams , S. Jacques , S.E. Denman , R.J. Eckard , J.L. Jacobs
{"title":"A bromoform based Investigational Veterinary Product fed twice daily to lactating dairy cows had no adverse effects on eating, rumination, or locomotion behaviours","authors":"R. Tognelli ,&nbsp;P.S. Alvarez-Hess ,&nbsp;A.S. ó Neachtain ,&nbsp;S. Chandra ,&nbsp;S.R.O. Williams ,&nbsp;S. Jacques ,&nbsp;S.E. Denman ,&nbsp;R.J. Eckard ,&nbsp;J.L. Jacobs","doi":"10.1016/j.vas.2025.100539","DOIUrl":"10.1016/j.vas.2025.100539","url":null,"abstract":"<div><div>An Investigational Veterinary Product (IVP) containing synthetic bromoform has emerged as a promising technology for reducing livestock enteric methane emissions. However, the effects of feeding the bromoform-based IVP on eating, rumination and locomotion behaviour (critical animal welfare indicators) remain unclear. This study assessed the response of supplementing twice daily an IVP at two bromoform inclusion rates to lactating dairy cows at milking on i) eating and rumination behaviour; and ii) locomotion behaviour. Thirty multiparous, Holstein-Friesian cows (214 ± 15.2 days in milk) were allocated to one of three treatments; CON (0 mg bromoform/cow per day), LowBR (227 mg bromoform/cow per day) and HighBR (455 mg bromoform/cow per day). The IVP was mixed with 3.1 kg dry matter of grain and offered during each milking. The basal diet consisted of vetch hay offered <em>ad libitum</em>. Cows were equipped with a RumiWatch System noseband pressure sensor and pedometer (ITIN + HOCH GmbH, Liestal, Switzerland) for 11 days. Data were analysed by CRD-based repeated measures split-plot ANOVA using Genstat 24 (VSN International, Hemel Hempstead, UK). Results showed no significant effect of the IVP on eating, rumination, lying or standing behaviour. Although cows fed the IVP showed an increase in walking duration, the difference was negligible in practical terms (twelve minutes per day). These findings indicate that feeding dairy cows with the bromoform-based IVP does not change their behaviour, resulting in a potential technology to reduce enteric methane emissions without compromising animal welfare.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100539"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145525325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Securidaca longipedunculata (violet tree) effect on semen quality and blood sex hormone levels in indigenous goats 紫罗兰树对地方山羊精液质量和血液性激素水平影响的评价
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-26 DOI: 10.1016/j.vas.2025.100531
Kamogelo Shai , Sogolo L. Lebelo , Freddy Manyeula , Monnye Mabelebele , Amenda N. Sebola
{"title":"Assessment of Securidaca longipedunculata (violet tree) effect on semen quality and blood sex hormone levels in indigenous goats","authors":"Kamogelo Shai ,&nbsp;Sogolo L. Lebelo ,&nbsp;Freddy Manyeula ,&nbsp;Monnye Mabelebele ,&nbsp;Amenda N. Sebola","doi":"10.1016/j.vas.2025.100531","DOIUrl":"10.1016/j.vas.2025.100531","url":null,"abstract":"<div><div>Semen quality, sperm characteristics and profiling of reproductive hormones of indigenous goat bucks (IGB) are of paramount importance for both the sustainable management of livestock populations and the economic well-being of farming communities. The current study assessed the impact of S.longipedunculata leaf extract (SLLE) on semen quality, sperm characteristics, and reproductive hormones in IGB. Sixteen pubertal bucks (12–18 months) were randomly assigned to four treatment groups receiving SLLE at 0, 50, 100, and 125 mg/kg body weight. Results showed that SLLE at 50 and 100 mg/kg improved spermatogenesis, increasing sperm concentration and semen volume. However, higher doses (125 mg/kg) reduced live sperm cell percentages. SLLE significantly influenced sperm motility and velocity but had no significant effect on oestrogen and testosterone levels. These findings suggest SLLE may enhance IGB reproductive performance.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100531"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential effects of flunixin meglumine and meloxicam on TNF- α production in LPS-stimulated equine neutrophils in vitro 氟尼新-大明与美洛昔康对体外lps刺激马中性粒细胞TNF- α生成的差异影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-09-21 DOI: 10.1016/j.vas.2025.100513
Shuntaro Urayama , Ryo Muko , Masanori Muranaka , Hiroshi Mita , Minoru Ohta , Hiroshi Matsuda , Akane Tanaka
{"title":"Differential effects of flunixin meglumine and meloxicam on TNF- α production in LPS-stimulated equine neutrophils in vitro","authors":"Shuntaro Urayama ,&nbsp;Ryo Muko ,&nbsp;Masanori Muranaka ,&nbsp;Hiroshi Mita ,&nbsp;Minoru Ohta ,&nbsp;Hiroshi Matsuda ,&nbsp;Akane Tanaka","doi":"10.1016/j.vas.2025.100513","DOIUrl":"10.1016/j.vas.2025.100513","url":null,"abstract":"<div><div>Systemic inflammatory response syndrome (SIRS) induced by endotoxemia is usually secondary to colitis and is a major cause of high morbidity and mortality in horses. Non-steroidal anti-inflammatory drugs (NSAIDs), such as flunixin meglumine (FM) and meloxicam (MX), are used to improve clinical outcomes in SIRS/endotoxemia. These NSAIDs suppress tumor necrosis factor-alpha (TNF-α) levels; however, the underlying mechanisms remain unclear. The aim of this study was to investigate the inhibitory effects of FM and MX on TNF-α in lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) and neutrophils <em>in vitro</em>. Blood samples were collected from three healthy thoroughbred horses, and PBMCs and neutrophils were isolated using density gradient centrifugation. Cells were cultured with LPS (0.3 ng/mL) and FM or MX (5, 10, or 50 μM). TNF-α gene expression was analyzed using quantitative real-time PCR, and protein levels were measured using ELISA. No significant inhibitory effects of FM or MX on TNF-α gene or protein expression were observed in LPS-stimulated PBMCs. However, FM significantly inhibited the increase in TNF-α protein levels in LPS-stimulated neutrophils in a concentration-dependent manner (<em>p</em> &lt; 0.05). MX showed a similar tendency in LPS-stimulated neutrophils, but the differences were not significant. The regulation of neutrophil-derived TNF-α by FM administration could be a promising therapeutic strategy for equine SIRS/endotoxemia, providing mechanistic insight for optimizing anti-inflammatory therapy.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100513"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a TaqMan probe-based dual real time PCR assay for the identification of NADC34-like PRRSV 基于TaqMan探针的双实时PCR方法鉴定nadc34样PRRSV的建立
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-08-27 DOI: 10.1016/j.vas.2025.100503
Shanshan Han , Tao Qin , Taichun Chen , Xiaocheng Xing , Qiang Zhang
{"title":"Development of a TaqMan probe-based dual real time PCR assay for the identification of NADC34-like PRRSV","authors":"Shanshan Han ,&nbsp;Tao Qin ,&nbsp;Taichun Chen ,&nbsp;Xiaocheng Xing ,&nbsp;Qiang Zhang","doi":"10.1016/j.vas.2025.100503","DOIUrl":"10.1016/j.vas.2025.100503","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus that induces reproductive disorders in sows and respiratory diseases in growing pigs. Recently, the NADC34-like strain of PRRSV has become more prevalent, with outbreaks occurring across pig farms in China. However, a reliable diagnostic method for the clinical detection of this strain has been absent. This study developed a TaqMan probe-based dual real-time quantitative PCR assay targeting the M and GP5 genes to specifically identify the NADC34-like PRRSV strain. The assay exhibited high specificity, detecting exclusively the NADC34-like strain without cross-reactivity with other PRRSV strains. The detection limits for pMD-M and pMD-GP5 plasmids were 2.67 × 10² and 1.35 × 10¹ copies/μL, respectively, indicating high assay sensitivity. The assay also demonstrated excellent reproducibility, with coefficient of variation (CV) values for both recombinant plasmids below 2 %. Among 251 clinical samples, 27 tested positive for NADC34-like PRRSV. This study establishes an accurate, sensitive, and reliable TaqMan dual real-time PCR assay for detecting NADC34-like PRRSV, offering a valuable tool for clinical diagnostics and outbreak management in pig farms.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100503"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Body condition score at calving, subclinical ketosis, postpartum body condition score losses, diseases, and fertility in Holstein cows: modelling confounding associations” “荷斯坦奶牛产犊时身体状况评分、亚临床酮症、产后身体状况评分损失、疾病和生育力:模型混淆关联”的勘误
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-08-19 DOI: 10.1016/j.vas.2025.100497
Pedro Melendez , Julian Bartolome , Gerardo Gonzalez , Gustavo Lastra-Duran , Pablo Pinedo
{"title":"Corrigendum to “Body condition score at calving, subclinical ketosis, postpartum body condition score losses, diseases, and fertility in Holstein cows: modelling confounding associations”","authors":"Pedro Melendez ,&nbsp;Julian Bartolome ,&nbsp;Gerardo Gonzalez ,&nbsp;Gustavo Lastra-Duran ,&nbsp;Pablo Pinedo","doi":"10.1016/j.vas.2025.100497","DOIUrl":"10.1016/j.vas.2025.100497","url":null,"abstract":"","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100497"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of dietary wine lees on oxidative stress, egg quality and yolk lipid stability in laying hens fed with a diet rich in n-3 polyunsaturated fats 饲粮中添加酒糟对饲粮富含n-3多不饱和脂肪蛋鸡氧化应激、蛋品质和蛋黄脂质稳定性的影响
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-09-15 DOI: 10.1016/j.vas.2025.100509
Daniel Mierlita , Stelian Dărăban , Adrian Macri , Ioan Mircea Pop
{"title":"Effect of dietary wine lees on oxidative stress, egg quality and yolk lipid stability in laying hens fed with a diet rich in n-3 polyunsaturated fats","authors":"Daniel Mierlita ,&nbsp;Stelian Dărăban ,&nbsp;Adrian Macri ,&nbsp;Ioan Mircea Pop","doi":"10.1016/j.vas.2025.100509","DOIUrl":"10.1016/j.vas.2025.100509","url":null,"abstract":"<div><div>Wine lees (WLs) are a by-product of the winemaking process, but the valorization of WLs as poultry feed has not yet been achieved. Accordingly, the aim of the present study was to evaluate the potential benefits of using WLs as a feed ingredient. To this end, we investigated their effects on serum and hepatic markers of oxidative stress and egg quality in laying hens susceptible to lipid peroxidation by feeding them diets enriched in n-3 PUFA. In addition, lipid profile and oxidative stability of the yolk during storage were evaluated. A total of 128 30-week-old Tetra SL laying hens were divided into four groups (n = 32): the negative control (C–) received a standard diet, while the three other groups (positive control, C+; WL2.5; and WL5) received the standard diet supplemented with 5 % linseed oil rich in n-3 PUFA. In the WL2.5 and WL5 groups, WLs were introduced into the diet at levels of 2.5 % and 5 %, respectively. The increased intake of n-3 PUFA induced oxidative stress, which was evidenced by increased malondialdehyde (MDA) and reduced serum and liver antioxidant enzyme activity. However, these parameters were significantly improved in hens supplemented with WLs. The reduction in n-3 PUFA content in stored eggs was &lt; 19 % in hens fed with WLs, while the reduction exceeded 33 % in the C+ group. In conclusion, WLs can improve egg production and egg quality (yolk color and cholesterol content), the antioxidant status of hens and the oxidative stability of eggs, with the possibility of extending their shelf life.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100509"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of ascorbic acid as an intervention of metal toxicity in dogs in Kabwe district 抗坏血酸对卡布韦地区犬金属毒性干预的评价
IF 1.9
Veterinary and Animal Science Pub Date : 2025-12-01 Epub Date: 2025-10-09 DOI: 10.1016/j.vas.2025.100519
Nelly Banda , Mahongo Selwa , Rio Doya , Nyein Chan Soe , Andrew Kataba , John Yabe , Golden Zyambo , Kaampwe Muzandu , Yared Beyene Yohannes , Yoshinori Ikenaka , Mayumi Ishizuka , Shouta MM Nakayama
{"title":"Evaluation of ascorbic acid as an intervention of metal toxicity in dogs in Kabwe district","authors":"Nelly Banda ,&nbsp;Mahongo Selwa ,&nbsp;Rio Doya ,&nbsp;Nyein Chan Soe ,&nbsp;Andrew Kataba ,&nbsp;John Yabe ,&nbsp;Golden Zyambo ,&nbsp;Kaampwe Muzandu ,&nbsp;Yared Beyene Yohannes ,&nbsp;Yoshinori Ikenaka ,&nbsp;Mayumi Ishizuka ,&nbsp;Shouta MM Nakayama","doi":"10.1016/j.vas.2025.100519","DOIUrl":"10.1016/j.vas.2025.100519","url":null,"abstract":"<div><div>Non-essential metals and metalloids are known to induce oxidative stress in exposed organisms, often leading to cellular damage and systemic toxicity. While chelation therapy remains the primary treatment for metal toxicity, its application is limited by side effects. L-ascorbic acid (L-AA), a widely available antioxidant, has emerged as a promising nutritional intervention for mitigating metal-induced oxidative stress. Dogs, whose blood lead levels (BLLs) closely mirror those of humans, have been utilized as sentinel species in environmental toxicology studies.</div><div>This study aimed to evaluate the therapeutic potential of L-AA in dogs residing in Kabwe, Zambia, a former mining town where remediation of heavy metal contamination is ongoing. The reported BLLs in Kabwe dogs ranged from 0.43 µg/dL to 123.5 µg/dL. A total of 22 dogs (10 females and 12 males) received oral L-AA supplementation daily for 14 days. Blood samples were collected on Day 1 of L-AA administration and Day 14 to assess biochemical and toxicological changes.</div><div>Post-treatment analysis revealed statistically significant reductions in plasma malondialdehyde, cortisol, blood urea nitrogen, and creatinine levels, as determined by Student’s t-test and Wilcoxon signed-rank test. Additionally, δ-aminolaevulinic acid dehydratase activity was significantly elevated, indicating improved oxidative status. These findings support the efficacy of L-AA in attenuating oxidative stress associated with metal and metalloid exposure, even in the absence of exposure cessation.</div><div>Interestingly, Pb levels declined predominantly in dogs younger than 24 months. Furthermore, reductions in Zn and Cu commonly linked to L-AA administration were observed exclusively in male dogs, suggesting a sex-specific response.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100519"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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