Veterinary and Animal Science最新文献

{"title":"Experimental long bone fracture healing in goats with cockle shell-based calcium carbonate bone paste","authors":"Saroj Kumar Yadav ,&nbsp;Subrata Kumar Shil ,&nbsp;Monoar Sayeed Pallab ,&nbsp;Kh. Nurul Islam ,&nbsp;Bibek Chandra Sutradhar ,&nbsp;Bhajan Chandra Das","doi":"10.1016/j.vas.2024.100374","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100374","url":null,"abstract":"<div><p>Long bone fractures are common orthopedic conditions. There are numerous ways to repair these fractures. Bone grafting becomes necessary when a broken bone has a significant gap. However, due to insufficient donor volume and donor site morbidity, substitutes are required. In veterinary orthopaedics, calcium carbonate from cockle shells could be used as a bone biomaterial. We investigated its efficacy as a bone biomaterial repair for goat femoral fractures. The study included 10 healthy adult male Black Bengal goats weighing 8 kg and aged 12–13 months. The study includes control and treatment groups. Intramedullary pinning stabilized an 8-mm right femur diaphyseal fracture in the treatment and control groups. The treated group received 2 ml of bone paste in the fractured gap, whereas the control group left it empty. We examined all goats with X-rays on the 7th, 45th, and 60th days, followed by gross and histological findings. Due to callus bridging, radiographs revealed faster bone growth in the treated group than in the control group. Gross examination demonstrates the treated group had a larger fracture callus than the control group. Histopathology showed that bone formed faster and included more osteocytes, osteoblasts, osteoclasts, and bony spicules than in the control group. The treated group had more periosteum osteoblasts, while the control group had fibroblasts. These results showed that the treated group had more osteogenic activity than the control group. This study demonstrates the potential of cockle shell-based calcium carbonate bone paste as a synthetic biomaterial for healing long bone fractures in goats.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100374"},"PeriodicalIF":1.9,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000413/pdfft?md5=5d71e3625728f8b6f82275ebbb82b82b&pid=1-s2.0-S2451943X24000413-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic predictions under different genetic architectures are impacted by mating designs","authors":"Sahar Ansari,&nbsp;Navid Ghavi Hossein-Zadeh,&nbsp;Abdol Ahad Shadparvar","doi":"10.1016/j.vas.2024.100373","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100373","url":null,"abstract":"<div><p>Mating in animal communities must be managed in a way that assures the performance increase in the progenies without increasing the rate of inbreeding. It has currently become possible to identify millions of single nucleotide polymorphisms (SNPs), and it is feasible to select animals based on genome-wide marker profiles. This study aimed to evaluate the impact of five mating designs among individuals (random, positive and negative assortative, minimized and maximized inbreeding) on genomic prediction accuracy. The choice of these five particular mating designs provides a thorough analysis of the way genetic diversity, relatedness, inbreeding, and biological conditions influence the accuracy of genomic predictions. Utilizing a stochastic simulation technique, various marker and quantitative trait loci (QTL) densities were taken into account. The heritabilities of a simulated trait were 0.05, 0.30, and 0.60. A validation population that only had genotypic records was taken into consideration, and a reference population that had both genotypic and phenotypic records was considered for every simulation scenario. By measuring the correlation between estimated and true breeding values, the prediction accuracy was calculated. Computing the regression of true genomic breeding value on estimated genomic breeding value allowed for the examination of prediction bias. The scenario with a positive assortative mating design had the highest accuracy of genomic prediction (0.733 ± 0.003 to 0.966 ± 0.001). In a case of negative assortative mating, the genomic evaluation's accuracy was lowest (0.680 ± 0.011 to 0.899 ± 0.003). Applying the positive assortative mating design resulted in the unbiased regression coefficients of true genomic breeding value on estimated genomic breeding value. Based on the current results, it is suggested to implement positive assortative mating in genomic evaluation programs to obtain unbiased genomic predictions with greater accuracy. This study implies that animal breeding programs can improve offspring performance without compromising genetic health by carefully managing mating strategies based on genetic diversity, relatedness, and inbreeding levels. To maximize breeding results and ensure long-term genetic improvement in animal populations, this study highlights the importance of considering different mating designs when evaluating genomic information. When incorporating positive assortative mating or other mating schemes into genomic evaluation programs, it is critical to consider the complex relationship between gene interactions, environmental influences, and genetic drift to ensure the stability and effectiveness of breeding efforts. Further research and comprehensive analyzes are needed to fully understand the impact of these factors and their possible complex interactions on the accuracy of genomic prediction and to develop strategies that optimize breeding outcomes in animal populations.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100373"},"PeriodicalIF":1.9,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000401/pdfft?md5=5ac9870c8f4841a86fe0372a16a6f5ac&pid=1-s2.0-S2451943X24000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing reproductive performance in pangasius catfish broodstock: A review of dietary and molecular strategies","authors":"Donald Torsabo ,&nbsp;Sairatul Dahlianis Ishak ,&nbsp;Noordiyana Mat Noordin ,&nbsp;Khor Waiho ,&nbsp;Ivan Chong Chu Koh ,&nbsp;Muhammad Abduh Yazed ,&nbsp;Ambok Bolong Abol-Munafi","doi":"10.1016/j.vas.2024.100375","DOIUrl":"10.1016/j.vas.2024.100375","url":null,"abstract":"<div><p><em>Pangasius</em> catfish, a significant player in the global whitefish market, encounters challenges in aquaculture production sustainability. Quality broodstock maintenance and seed production are impeded by growth, maturation, and fecundity issues. This review investigates the efficacy of strategic nutrient composition and molecular strategies in enhancing broodstock conditions and reproductive performance across various fish species. A notable knowledge gap for <em>Pangasius</em> catfish hampers aquaculture progress. The review assesses nutrient manipulation's impact on reproductive physiology, emphasizing <em>pangasius</em> broodstock. A systematic review analysis following PRISMA guidelines was conducted to identify research trends and hotspots quantitatively, revealing a focus on <em>P. bocourti</em> and fertilization techniques. Addressing this gap, the review offers insights into dietary nutrients manipulation and genetic tool utilization for improved seed production, contributing to <em>pangasius</em> catfish aquaculture sustainability.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100375"},"PeriodicalIF":1.5,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000425/pdfft?md5=71775ea3b185cb45c79b7de4fc4fb1d1&pid=1-s2.0-S2451943X24000425-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141398103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cajanus cajan and Lablab purpureus leaf meal-potential supplements over conventional protein sources for yearling Horro sheep fed a basal diet of fodder oat (Avena sativa) hay","authors":"Abuye Tulu,&nbsp;Worku Temesgen,&nbsp;Tusa Gemechu,&nbsp;Birmeduma Gadisa,&nbsp;Mekonnen Diribsa","doi":"10.1016/j.vas.2024.100376","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100376","url":null,"abstract":"<div><p>A 90-day feeding trial with 25 yearling Horro lambs (22.6 ± 1.8 kg) was conducted from November 2022 to January 2023. The trial was designed to see how supplementing forage varieties of <em>Cajanus cajan</em> (Degagsa and Belabas) and <em>Lablab purpureus</em> (Beresa-55 and Gebis-17) affected the growth performance, feed intake and digestibility of Horro lambs fed a basal diet of fodder oat hay compared to conventional protein supplements. Five experimental sheep per treatment were arranged in a Randomized Complete Blocked Design to receive dietary treatments formulated on an iso-nitrogenous basis which was targeted to provide crude protein (CP) of 64.37 g/h/day. Data were taken on weight gain, feed conversion ratio, feed intake and refusal, and in-vivo digestibility features. All lambs were fed <em>ad-libitum</em> fodder oat hay containing 91.5 % Dry matter, 7.8 % ash, 6.4 % crude protein, 60 % neutral detergent fiber, 42.8 % acid detergent fiber, 13.4 % acid detergent lignin and 65 % in-vitro organic matter digestibility. The dietary treatments were fodder oat hay plus 280.8 g Gebis-17 variety (T1), 274.4 g Beresa-55 variety (T2), 320.9 g Belabas variety (T3), 329.3 g Degagsa variety (T4) and 300 g concentrate mixture (T5). The total dry matter intake (DMI) was higher in T3 and T4 than in the other treatment groups. Except for CP, nutritional consumption differed significantly across treatments. T3 and T4 had higher fiber intakes than the other treatments, while T2 had higher ash intakes and T5 had higher metabolizable energy intakes. Even though there was no significant variation in final body weight, lambs in T5 and T1 showed the greatest weight change and average daily gain. Although the diet in T5 induced the lamb performance in a manner comparable to that of the other dietary supplements, its inaccessibility and high cost would limit the use of such supplements by smallholder farmers. Thus, depending on their availability, either <em>Lablab purpurues</em> or <em>Cajanus cajan</em> varieties could be employed as feed supplements in the lambs` diet.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100376"},"PeriodicalIF":1.9,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000437/pdfft?md5=5df03f52128955ca9a7f7a65f0155e37&pid=1-s2.0-S2451943X24000437-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia","authors":"Jeiczon Jaimes-Dueñez,&nbsp;Eyner Goyeneche-Ortiz,&nbsp;Marisol Tique-Oviedo,&nbsp;Melissa C Ortiz-Pineda,&nbsp;Luis Cardenas-Pinto,&nbsp;Angela Patricia Jimenez-Leaño,&nbsp;Julian Ruiz-Saenz","doi":"10.1016/j.vas.2024.100372","DOIUrl":"10.1016/j.vas.2024.100372","url":null,"abstract":"<div><p>Enzootic Bovine Leukosis (EBL), caused by the bovine leukosis virus (BLV), is a global infectious disease affecting livestock. This study focuses on studying the frequency and genetic traits of BLV in three Creole breeds including Chino Santandereano (Chino), Casanareño (CAS), and Sanmartinero (SM) in Eastern Colombia. We implemented a cross-sectional survey between 2019 and 2020 across four departments (Arauca, Casanare, Santander and Meta) in Eastern Colombia to assess the molecular characteristics of BLV infection in these breeds. A total of 253 cattle were analyzed, of which 42.6 %, 28.8 %, and 28.4 % belonged to the Chino, CAS, and SM breeds, respectively. BLV provirus was detected using nested polymerase chain reaction (n-PCR) targeting the conserved region of the <em>env</em> viral gene. Subsequently, the obtained amplicons were sequenced and subjected to phylogenetic analyses. The overall BLV infection frequency was 26.48 % (95 % CI: 21.01 – 31.98 %), with Chino exhibiting the highest frequency (35.1 %) following by SAM and CAS, respectively (<em>P</em> &lt; 0.05). Other epidemiological variables associated with the infection included age, department, and season (<em>P</em> &lt; 0.05). BLV-positive animals exhibited elevated levels of total serum proteins (<em>P</em> &lt; 0.05), while molecular characterization revealed the exclusive circulation of BLV genotype 1 within these breeds. This study provides an updated assessment of BLV infection in Creole breeds from the eastern of Colombia, underscoring their lower infection frequency compared to introduced breeds and their reduced susceptibility to developing clinical signs. The epidemiological and molecular characteristics observed should be considered in developing control programs aimed at improving genetic resistance to BLV in Colombian cattle.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100372"},"PeriodicalIF":1.9,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000395/pdfft?md5=5402d965f37ba4f88cc99592abdbf324&pid=1-s2.0-S2451943X24000395-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141390199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time budgets differ in horses during continuous and space-restricted rotational grazing","authors":"Beverly Gartland ,&nbsp;William Strunk ,&nbsp;Bruce Schulte ,&nbsp;Fred DeGraves ,&nbsp;Jim Koostra","doi":"10.1016/j.vas.2024.100371","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100371","url":null,"abstract":"<div><p>Horses can become obese and develop related health issues such as laminitis from excessive grazing on high-quality pasture grass; limiting pasture intake can allow weight loss to occur. The objective of this study was to determine the effect of space-restricted rotational grazing on body weight (BW) and time budgets in horses. Eight mature geldings and mares with maintenance-only requirements were randomly assigned to either a space-restricted rotational grazing group (SRG; BW 512 ± 6 kg; <em>n</em> = 4) or a continuous grazing group (CG; BW 517 ± 49 kg; <em>n</em> = 4) for 42 d SRG horses grazed an area with dimensions to provide 80–90 % of mean digestible energy requirement for the 4 horses over a 7-d grazing period; whereas, the CG horses continuously grazed similar non-toxic endophyte-infected tall fescue pasture providing greater than maintenance requirements for the 42 d Horses in the SRG group were moved to a new area every 7 d for 6 weeks. On d 7 at 1600 h of each week, horses were brought inside, and feed was withheld overnight. At 0700 h the next day, BWs were recorded prior to turnout. Observers recorded behaviors simultaneously on SRG and CG horses every six minutes throughout the day three days per week according to an ethogram. This included 30 s scans of all horses. Proportion of grazing and standing had an inverse relationship. Proportion of grazing was affected by the treatment by time interaction, which grazing was displayed more in SRG than CG during weeks 2 and 3, and then reversed weeks 4, 5 and 6.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100371"},"PeriodicalIF":1.5,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000383/pdfft?md5=9b3c1e1e0f031e7264bc5ec0b870edff&pid=1-s2.0-S2451943X24000383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141324437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of probiotic-supplemented milk replacer on growth, blood biochemistry, fermentation, digestibility, and carcass traits in lambs","authors":"SH Mousavi Esfiokhi,&nbsp;MA Norouzian,&nbsp;MR Sahl Abadi,&nbsp;MR Rezaei Ahvanooei","doi":"10.1016/j.vas.2024.100368","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100368","url":null,"abstract":"<div><p>The objective of this study was to investigate the effects of feeding cow's milk replacer and probiotic supplementation on growth performance, blood metabolites, ruminal fermentation parameters, and nutrient digestibility in male lambs, with ewe's milk serving as the control treatment. Eighteen male lambs with an average initial body weight of 5.0 ± 1.2 kg and age of 15 ± 4 days were randomly assigned to three experimental groups: ewe's milk, cow's milk replacer, and cow's milk replacer with probiotic supplementation. The experiment was conducted using a completely randomized design. Lambs fed ewe's milk showed significantly higher average daily gain (218.4 g/day) compared to those fed cow's milk replacer (183.7 g/day) or cow's milk replacer with probiotic (209.1 g/day). Similarly, dry matter intake was highest in the ewe's milk group (585.6 g/day) compared to the cow's milk replacer (435.9 g/day) and cow's milk replacer with probiotic (510.5 g/day) groups. Blood glucose levels were higher in the ewe's milk group (75.3 mg/dL) compared to the cow's milk replacer (70.3 mg/dL) and cow's milk replacer with probiotic (72.1 mg/dL) groups. Probiotic supplementation resulted in increased blood urea nitrogen (15.6 mg/dL) and total protein (7.3 g/dL) levels compared to the other groups. Furthermore, the ewe's milk group showed higher apparent dry matter (76.1%) and crude protein (68.5%) digestibility compared to the other treatments. The fecal score on day 30 was higher in the cow's milk replacer group (2.34) compared to the ewe's milk (1.24) and cow's milk replacer with probiotic (1.45) groups. There were no significant differences in the carcass traits of the experimental lambs. In conclusion, based on the results of this study, it seems that feeding cow's milk + probiotic as a replacement for ewe's milk did not significantly affect the performance, carcass traits, and blood and fermentation parameters in infant lambs.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100368"},"PeriodicalIF":1.5,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000358/pdfft?md5=e9eb3b31b47801d0eae819b300224837&pid=1-s2.0-S2451943X24000358-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141303809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the digestibility, growth performance, hematological and serum biochemical profile of Bandjock Local Pigs (BLP) and Duroc X Large White pigs (DLW)","authors":"Sandra Olivia Magne Ghomsi ,&nbsp;Sylvain Nsangou Pechangou ,&nbsp;Regine Sandra Maafo ,&nbsp;Hippolyte Tene Mouafo ,&nbsp;Agbor Kingsley Etchu ,&nbsp;Felix Charles Bilong Bilong ,&nbsp;Paul Fewou Moundipa","doi":"10.1016/j.vas.2024.100370","DOIUrl":"10.1016/j.vas.2024.100370","url":null,"abstract":"<div><p>The study aimed to assess the digestibility, growth performance, and selected biochemical and hematological parameters of Bandjock Local pigs (BLP) and Duroc X Large White pigs (DLW). We hypothesize that the use of local ingredients in diet formulation associated to improved rearing conditions, enhance the growth performance, digestibility, biochemical and hematological parameters of BLP. The trial involved dividing twenty-four eight-week-old weaner pigs into two groups. Each group was randomly assigned to separate pig units. Animal management and feeding were consistent between both groups. The trial lasted for 120 days during which the growth performance, the digestibility of ingested nutrients, and biochemical and hematological parameters were evaluated. The results indicate that DLW pigs registered a weight gain (46.56±5.19 kg) significantly higher than BLP pigs (34.02), with a statistically similar feed conversion ratio. Except for albumin which was significantly higher in DLW and urea in BLP, there were no significant differences (<em>p</em> &gt; 0.05) in total protein, cholesterol, globulin, triglycerides, creatinine, AST, and ALT as well as hematological parameters between the breeds. However, the exotic DLW had a relatively high mean blood platelets and white blood cell concentration. Although the fecal nitrogen was higher in BLP and the urinary nitrogen was lower in the DLW, there were significant differences (<em>p</em> &lt; 0.05) for urine, fecal, and total excreted nitrogen in both breeds. The lowest scores in nutrient digestibility were recorded with BLP compared to DWL, except for crude fibers. The results of this study demonstrated that using diet formulated with local ingredients and improved rearing conditions, Cameroonian local indigenous pigs have a better productivity compared to exotic breeds. It suggests that efforts should be made by authorities to promote and valorize the rearing of indigenous pigs in complement to exotic pig breeds in view of strengthening the Cameroonian pig industry.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100370"},"PeriodicalIF":1.5,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000371/pdfft?md5=345247319eaa704868f45fd2aaecddbc&pid=1-s2.0-S2451943X24000371-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of recombinant Ybgf in a double antigen-ELISA for the detection of Coxiella antibodies in ruminants","authors":"Gianmarco Ferrara ,&nbsp;Barbara Colitti ,&nbsp;Flores-Ramires Gabriela ,&nbsp;Sergio Rosati ,&nbsp;Giuseppe Iovane ,&nbsp;Ugo Pagnini ,&nbsp;Serena Montagnaro","doi":"10.1016/j.vas.2024.100366","DOIUrl":"10.1016/j.vas.2024.100366","url":null,"abstract":"<div><p>Q fever is a zoonosis whose main reservoirs are domestic ruminants. Surveillance in these species is carried out mainly with serological tests, which, however, have limited diagnostic performance, and their manufacturing requires laboratories equipped with high biosafety requirements for antigen production. Recombinant ELISAs do not depend on these requirements and, being based on a single antigen, can reduce potential false positivity by identifying antibodies specific to a phase of infection. The aim of this study was to apply a new technology (dual antigen test) to a recombinant protein (Ybgf), an antigen produced in recombinant form and already used in previous studies for the design of an indirect ELISA. The successfully produced recombinant antigen was used to coat 96-well plates and, at the same time, another antigen aliquot was conjugated with HRP to obtain an HRP-conjugated Ybgf. After setting the test conditions, the results obtained with the recombinant double antigen test were compared with those obtained with a commercial assay (considered as reference assay) testing a total of 514 ruminant samples (280 goats and 234 cattle). A concordance of 86.2 and a Cohen's Kappa value of 0.72 were obtained, with no significant difference between the two species tested. Notably, the test proved to be highly specific, having correctly identified 250 out of 253 animals. This research represents an additional effort to use recombinant antigens to enhance serological methods in veterinary medicine. In a “one-health scenario”, improving the performance of serological tests used in veterinary practice also means improving the surveillance of this infection.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100366"},"PeriodicalIF":1.5,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000334/pdfft?md5=6ae62c96b7bc775f49399e0806b91e6a&pid=1-s2.0-S2451943X24000334-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141281034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh","authors":"Jarin Al Naser ,&nbsp;Hemayet Hossain ,&nbsp;Md. Shahidur Rahman Chowdhury ,&nbsp;Nasrin Akter Liza ,&nbsp;Rayhan Mahmud Lasker ,&nbsp;Asikur Rahman ,&nbsp;Md. Ariful Haque ,&nbsp;Md. Mukter Hossain ,&nbsp;Md. Mahfujur Rahman","doi":"10.1016/j.vas.2024.100367","DOIUrl":"10.1016/j.vas.2024.100367","url":null,"abstract":"<div><p>The emergence of <em>Salmonella enterica</em> serovars that produce extended-spectrum beta-lactamase and exhibit multi-drug resistance (MDR) poses a substantial global threat, contributing to widespread foodborne illnesses and presenting an alarming issue for public health. This study specifically concentrated on the isolation and identification of ESBL-resistant genes (<em>bla</em>_TEM, <em>bla</em>_SHV, <em>bla</em>_CTX-M1, <em>bla</em>_CTX-M2, <em>bla</em>_CTX-M9, <em>MultiCase</em>_ACC, <em>MultiCase</em>_MOX, <em>MultiCase</em>_DHA, <em>bla</em>_OXA) and the antibiogram profiling of <em>Salmonella enterica</em> serovars found in goat meat samples procured from retail outlets in Bangladesh. During the research in the Sylhet district of Bangladesh, researchers gathered a total of 210 samples of goat meat from 13 different Upazilas. Primarily, cultural and biochemical methods were used for isolation of bacteria from the selected samples. <em>Salmonella enterica</em> serovars Typhimurium and Enteritidis, along with three ESBL-resistant genes, were identified through polymerase chain reactions (PCRs). The disk diffusion test was used to determine antimicrobial susceptibilities. Out of 210 samples analysed, <em>Salmonella</em> spp<em>.</em> was detected in 18.10 % (38 out of 210), with <em>S.</em> Enteritidis and <em>S.</em> Typhimurium found in 9.05 % (19 out of 210) and 5.24 % (11 out of 210) of the samples, respectively. A total of 72.73 % (8/11) of <em>S.</em> Enteritidis and 100 % (19/19) of <em>S.</em> Typhimurium isolates were positive by Multidrug-resistant patterns. The positive outcomes were found of <em>S.</em> Typhimurium tested 63.16 % (12 out of 19) for the <em>bla</em>_TEM gene and 21.05 % (4/19) for the <em>bla</em>_SHV, gene. The study proposes that the retail goat meat market channel could be a prominent transmission way of ESBL-producing MDR <em>Salmonella enterica</em> serovars, representing a significant public health hazard.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100367"},"PeriodicalIF":1.5,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000346/pdfft?md5=0fda53a5833e587404ff5adb1fd2dcdb&pid=1-s2.0-S2451943X24000346-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}