Veterinary and Animal Science最新文献

{"title":"Natural infection with Trypanosoma cruzi in a litter of dogs (Canis lupus familiaris) from Floridablanca, Colombia, suggests congenital transmission and a potential zoonotic risk","authors":"Jeiczon Jaimes-Dueñez ,&nbsp;María Victoria Esteban-Mendoza ,&nbsp;Ian Sebastián Murcia-Cueto ,&nbsp;Luz Zoraya Beatriz Duarte-Rodríguez ,&nbsp;Genny Liliana Uribe Gómez ,&nbsp;Harley Díaz Rincón ,&nbsp;Javier Alonso Herrera Cuadros ,&nbsp;Omar Cantillo-Barraza","doi":"10.1016/j.vas.2025.100562","DOIUrl":"10.1016/j.vas.2025.100562","url":null,"abstract":"<div><div>Chagas disease (CD) remains a major public health challenge in endemic regions of Colombia, where both domestic and sylvatic host sustain transmission. This study reports the first natural infection with <em>Trypanosoma cruzi</em> in a litter of dogs from Floridablanca, in the Metropolitan Area of Bucaramanga (MAB), Santander, most likely associated with congenital transmission. Eight puppies and their dam were clinically evaluated, and blood samples were analyzed through hematology, quantitative real-time PCR (qPCR), multiplex PCR for discrete typing units (DTUs) identification, and hemoculture. Serological surveillance was also performed in the owners’ nuclear family. All puppies and the dam tested positive for <em>T. cruzi</em> DNA by qPCR, with high parasitemia levels (751.8–977.3 parasite equivalents/mL in puppies and 526.2 parasite equivalents/mL in the dam), corresponding to DTU I (TcI). Hematological findings in puppies indicated regenerative anemia, thrombocytopenia, and lymphocytosis. Despite supportive treatment, seven puppies died (mortality rate = 87.5 %), and one was euthanized after three months of disease progression. Postmortem examination revealed multisystemic lesions, including interstitial pneumonia, necrotizing hepatitis, myocardial lymphocytic infiltrates with amastigote cysts, and meningoencephalitis, consistent with acute trypanosomiasis. Hemoculture was positive in this case. None of the family members tested positive for <em>T. cruzi</em> antibodies. To our knowledge, this is the first description of natural infection with <em>T. cruzi</em> DTU I (TcI) in a litter of dogs from Colombia. The marked parasitemia observed, together with hemoculture positivity, highlights the potential of these hosts to infect triatomine vectors and increase the risk of CD transmission in endemic areas.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100562"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of an optimized dairy calf-rearing protocol on performance and health in the subsequent fattening period on Swiss veal farms","authors":"Julia Rell ,&nbsp;Michael Walkenhorst ,&nbsp;Mirjam Holinger ,&nbsp;Corinne Bähler ,&nbsp;Jens Becker ,&nbsp;Martin Kaske","doi":"10.1016/j.vas.2026.100590","DOIUrl":"10.1016/j.vas.2026.100590","url":null,"abstract":"<div><div>We conducted a prospective field study to characterize the effects of an optimized rearing protocol of calves on dairy-farms on the subsequent performance, health and antimicrobial use (AMU) at the veal-farm. On 19 dairy-farms a total of 55 matched pairs of calves (same gender and breeding type, born within the same period and farm) were randomly assigned to the treatment group P (“preconditioned”) or C (“control”) during five two-week enrollment periods. The P-calves were fed milk ad libitum, received parenteral selenium and iron supplementation, wore calf jackets at low temperatures, and were vaccinated intranasally against bovine respiratory disease. The C-calves were fed restrictively and received no additional measures. At an average of 30 days of life, calves were transported directly to one of two veal-farms. Body weight and health score were determined between days 2 and 5 of life, at transport and in weeks 4 and 12 after arrival on the veal farm. All applications of antimicrobials were recorded. Mixed-effect models were applied for statistical analyses. While born with similar body weights, daily weight gain of the calves differed between groups on the dairy-farms (P: 1.10[0.96–1.25] vs. C: 0.70[0.55–0.84]kg/day; <em>p</em> &lt; 0.001) but not on the veal-farms (P: 1.34[1.24–1.44] vs. C: 1.29[1.19–1.39]kg/day). A similar carcass weight was reached in shorter time for P-calves compared to C-calves (104[99–110] vs. 115[109–120] days on the veal-farm; <em>p</em> = 0.04). Neither clinical health nor AMU differed between the groups. Thus, while preconditioning was effective regarding performance parameters, a significant advantage in respect of health status and AMU could not be demonstrated.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100590"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147419782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Feline lymphoma associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) infections in Brazil- Systematic review [Veterinary and Animal Science volume 30 (December 2025) 100514]","authors":"Pedro Antônio Bronhara Pimentel ,&nbsp;Antonio Giuliano ,&nbsp;Fernanda Vieira Amorim da Costa ,&nbsp;Paweł Marek Bęczkowski ,&nbsp;Letícia Neves Ribeiro ,&nbsp;Breno Neves Manzalli Oliveira ,&nbsp;Raquel Fernandes Pereira ,&nbsp;Rodrigo dos Santos Horta","doi":"10.1016/j.vas.2025.100547","DOIUrl":"10.1016/j.vas.2025.100547","url":null,"abstract":"","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100547"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147419783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Reproductive status effects on intraocular pressure in Mares, Veterinary and Animal Science 30 (2025) 100542","authors":"Sara Binesh ,&nbsp;Hamid Ghasemzadeh-nava ,&nbsp;Afshin Raoofi ,&nbsp;Seyed Mehdi Rajaei","doi":"10.1016/j.vas.2025.100552","DOIUrl":"10.1016/j.vas.2025.100552","url":null,"abstract":"","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100552"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147419784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of lignocellulose promotes the growth of Cherry Valley ducks by improving intestinal function","authors":"Xinzhi Geng ,&nbsp;Zhenzhen Chen ,&nbsp;Jian Wang ,&nbsp;Biao Dong ,&nbsp;Jing Ge ,&nbsp;Minmeng Zhao ,&nbsp;Long Liu ,&nbsp;Daoqing Gong ,&nbsp;Haixia Liu ,&nbsp;Tuoyu Geng","doi":"10.1016/j.vas.2025.100558","DOIUrl":"10.1016/j.vas.2025.100558","url":null,"abstract":"<div><div>Dietary fiber contributes to improving intestinal morphology, barrier integrity, and microbial balance, thereby enhancing nutrient utilization and growth performance in animals. This study evaluated the effects of supplementing lignocellulose on intestinal function and growth performance in meat ducks. A total of 180 one-day-old Cherry Valley ducks were randomly assigned to the control group (CON), lignocellulose group 1 (LC1), and lignocellulose group 2 (LC2) (6 replicates each group, 10 ducks each replicate). Ducks in the CON group were fed a basal diet; the LC1 group received the basal diet supplemented with 0.6% lignocellulose from days 1 to 42, while the LC2 group received the basal diet supplemented with 0.3 % lignocellulose from days 1 to 21 and 0.6 % from days 22 to 42. Lignocellulose supplementation increased average daily gain in the LC1 group (<em>P</em> &lt; 0.05), and improved feed conversion ratio in the LC2 group before day 28 (<em>P</em> &lt; 0.05). Additionally, lignocellulose increased intestinal length (<em>P</em> &lt; 0.05) and weight (<em>P</em> &lt; 0.05), and improved the intestinal structure (<em>P</em> &lt; 0.05) and antioxidant capacity (<em>P</em> &lt; 0.05). However, the expression of genes associated with tight junctions, immunity, and inflammation was not significantly affected. Overall, dietary lignocellulose promotes growth in meat ducks by improving intestinal morphology and antioxidant capacity without affecting the expression of genes involved in intestinal tight junction and immunity or inflammation. The magnitude of benefits depends on the supplementation level, duration, and the age of birds.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100558"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New frontiers in regenerative medicine: Protocol standardization and morphological assessment of leukocyte-platelet rich fibrin in cows, with a longitudinal study on growth factor release dynamics","authors":"Giovanni Della Valle ,&nbsp;Maria Chiara Alterisio ,&nbsp;Jacopo Guccione ,&nbsp;Chiara Caterino ,&nbsp;Federica Aragosa ,&nbsp;Gianmarco Ferrara ,&nbsp;Davide De Biase ,&nbsp;Paolo Ciaramella ,&nbsp;Gerardo Fatone","doi":"10.1016/j.vas.2025.100564","DOIUrl":"10.1016/j.vas.2025.100564","url":null,"abstract":"<div><div>Leukocyte-Platelets Rich Fibrin (L-PRF) is a platelet-derived autologous agent. It is a powerful wound-sealing biological scaffold that promotes the healing process through the release of growth factors (GFs) and matrix proteins. To date, the <span>l</span>-PRF in bovines has not yet been investigated and a standardized production protocol has not yet been described. Therefore, the aim of the study was to standardise the <span>l</span>-PRF production protocol and to evaluate macroscopically and histologically the <span>l</span>-PRF membranes obtained and observe the GFs release over time and lactation phases: at 1 h (T0), 4 h (T1), 24 h (T2), and 168 h (T3) after extraction. Eighty cows were divided into four groups of 20 animals, based on their lactation phase. A whole blood sample of 20 ml was taken and divided for <span>l</span>-PRF clot production and haemato-biochemical investigations. The membranes presented the following overall mean characteristics: a length of 34.4 ± 7.3 cm, a width of 12.5 ± 2.6 cm, and a weight of 4.9 ± 0.17 cm. Histological analysis showed a well-defined histoarchitecture consisting of a first layer of erythrocytes, a layer of leukocytes, erythrocytes, and platelets, a layer of leukocytes, a layer of platelets, and finally a layer of slightly eosinophilic fibrin. The GFs significantly increased with upward trends in all phases of lactation and at each time control, overlapping the behavior of GF in other species. Based on the encouraging findings of this study, further clinical trials for applicative potential of the <span>l</span>-PRF membrane in bovine medicine should be assessed.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"31 ","pages":"Article 100564"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Typing identification and biological characterization of Clostridium perfringens toxins associated with lamb dysentery in Xizang sheep","authors":"Zhuo Ma,&nbsp;Yuan Zhenjie","doi":"10.1016/j.vas.2025.100501","DOIUrl":"10.1016/j.vas.2025.100501","url":null,"abstract":"<div><div>The objectives of this study were to investigate the etiology of morbidity and mortality in Tibetan sheep from a township in Shannan, Tibet, and to characterize the biological characteristics of the causative pathogen, thereby providing a scientific basis for effective disease prevention and control. Pathological specimens were aseptically collected from deceased animals, and the pathogen was isolated and cultured based on clinical presentations. Identification of the isolate involved morphological observation, 16S rRNA PCR amplification and sequencing, biochemical assays, toxin gene typing, Kirby-Bauer disk diffusion antimicrobial susceptibility testing, and antibiotic resistance genes screening. On tryptose sulfite cycloserine (TSC) agar, the organism formed black, circular colonies. Gram staining revealed short, straight, Gram-positive bacilli arranged singly or in pairs, consistent with the <em>Clostridium perfringens</em> . 16S rRNA gene sequence alignment showed &gt;99 % identity to reference <em>C. perfringens</em> sequences in the NCBI database.</div><div>Confirming the strain asC. <em>perfringens</em>. Biochemical tests indicated positive reactions for glucose, Biochemical assays were positive for glucose, lactose, and maltose fermentation; gelatin hydrolysis; oxidase activity; nitrate reduction;hydrogen sulfide production; and ornithine decarboxylase, but negative results for indole production, mannitol and sorbitol fermentation, and urea hydrolysis, matching known traits of <em>C. perfringens</em>. . Toxin gene PCR detected <em>cpa,cpb</em>, and <em>etx,</em> but not <em>iap, cpe</em>, or <em>netB</em>, identifying the isolate as <em>C. perfringens type B</em>. Antimicrobial susceptibility testing revealed high sensitivity to sulfadiazine, norfloxacin, nitrofurantoin, and cefazolin, but resistance to amoxicillin, oxacillin, carbenicillin, and tetracycline.Among the resistance genes screened, only <em>aac(6′)-Ib-cr</em> was detected.This study reports the first isolation of C. perfringens type B in Shannan, Tibet; elucidates its biological characteristics and toxin profile; identifies effective antibiotics for targeted intervention; and provides foundational data for controlling lamb dysentery caused by this pathogen and for understanding its pathogenicity in sheep.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100501"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of postbiotics on the production parameters of rearing goose","authors":"Stella Szabó ,&nbsp;Mária Kovács-Weber ,&nbsp;Tibor István Pap ,&nbsp;Ágnes Dolányi ,&nbsp;Ferenc Pajor ,&nbsp;Ákos Bodnár ,&nbsp;Tamás Vajda ,&nbsp;Mónika Heincinger ,&nbsp;Rubina Tünde Szabó","doi":"10.1016/j.vas.2025.100541","DOIUrl":"10.1016/j.vas.2025.100541","url":null,"abstract":"<div><div>Our aim was to measure the mortality, live body weight, feed conversion, intake and detect the weight and stiffness of the liver, liver colour, breast and thigh weight as an effect of the dry form of Burain® postbiotic supplementation in rearing goose. The goslings were allocated into 5 equal groups: control (without supplementation), group 1 (1 g/kg postbiotic during the starter and grower period), group 2 (2 g/kg postbiotic during the starter and grower period), group 3 (0.2 g/kg postbiotic during the starter and grower period), group 4 (1 g/kg postbiotic during the starter period). Granulated feed and drinking water were provided ad libitum to all rearing goose. Supplementation significantly increased body weight in group 2 (6321.84±664.9 g) on week 8. Group 2 showed a significantly reduced feed conversion ratio (1.51±0.04), while groups 1 and 4 exhibited significantly lower daily feed intake than the control during the starter phase. There were no significant differences among groups in foie gras parameters or in thigh and breast weights. The liver weight and stiffness were found to be significantly positively correlated in each treatment group. The supplementation made a significant difference in the parameters of b* and L* for liver colour, but not for a* values. The impact of postbiotic supplementation in the rearing period is extended to weeks. Further investigations are needed to connect information from rearing to the fattening phase and the gut microbiome in goose.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100541"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145576292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiropractic evaluation in newborn foals: A preliminary study","authors":"Ilaria Arena ,&nbsp;Silvia Signor ,&nbsp;Jole Mariella ,&nbsp;Aliai Lanci ,&nbsp;Francesca Freccero ,&nbsp;Castagnetti Castagnetti","doi":"10.1016/j.vas.2025.100495","DOIUrl":"10.1016/j.vas.2025.100495","url":null,"abstract":"<div><div>This paper describes the more frequent chiropractic alterations in healthy and sick foals. The assessment is performed through a motion palpation exam, which locates the hypomobile joints along the skeleton. The motion palpation exam allowed the identification of multiple hypomobile areas in neonatal foals. The sick foals presented a statistically significant higher number of hypomobile joints in the cervical, thoracic, lumbar regions and limbs compared to the healthy foals. The most frequent alterations in sick foals involved the atlantooccipital joint, C5-C6, T14-T15, L5-L6, the sacrococcygeal joint and the scapulohumeral joint. In both groups, high prevalence of alterations occurred at the cervical and sacroiliac joints. The results show that some regions can be highly stressed during the perinatal period, especially in sick foals. Possible explanations are discussed. Chiropractic evaluation and subsequent manipulation could be routinely used to improve neonatal health, especially in sick foals.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100495"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular detection of Francisella tularensis and investigation of Coxiella burnetii in horse sera in Iran","authors":"Mehdi Narouei,&nbsp;Heidar Rahimi,&nbsp;Khatereh Kafshdouzan","doi":"10.1016/j.vas.2025.100529","DOIUrl":"10.1016/j.vas.2025.100529","url":null,"abstract":"<div><div>Infections caused by <em>Francisella tularensis</em> and <em>Coxiella burnetii</em>, as zoonotic diseases, pose a serious threat to the health of humans and animals. To date, there is limited information regarding these diseases in horses. This study aimed to evaluate the prevalence of <em>F. tularensis</em> and <em>C. burnetii</em> in the serum of racehorses in Iran (Golestan province). 350 blood samples were collected from racehorses in four regions of Golestan province, and demographic data (sex, age, and sampling location) were recorded. The collected serum samples were examined by PCR to identify the genomes of <em>F. tularensis</em> and <em>C. burnetii</em>. The results showed that 3.4 % (P &lt; 0.05, 95 % CI: 1.97 % – 5.9 %) of the serum samples were positive for <em>F. tularensis</em> genome, while no positive cases for <em>C. burnetii</em> genome were detected. Additionally, a significant relationship was observed between horse age and <em>F. tularensis</em> infection, with the highest prevalence (3.93 %) detected in animals younger than five years (P &lt; 0.05, 95 % CI: 2.21 % – 6.9 %). Moreover, the study revealed a significant difference in <em>F. tularensis</em> prevalence between sexes, with infection rates of 5.84 % in stallions and 1.88 % in mares. Statistical analysis showed no significant difference between the regions studied and the prevalence of <em>F. tularensis</em>. According to our knowledge, this is the first report of tularemia prevalence in horses in Iran. This study indicates that horses can be considered a potential weak reservoir for <em>F. tularensis</em>.</div></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"30 ","pages":"Article 100529"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}