Diana Fanelli , Rebecca Moroni , Giulia Sala , Pierre Melanie , Ilaria Tarabella , Nicole Telleschi , Saverio Maltinti , Mario Giorgi , Giovanni Barsotti , Fabrizio Passamonti , Paola Marmorini , Alessandra Rota , Francesco Camillo , Duccio Panzani
{"title":"Impact of low-dose ozone supplementation on motility parameters and bacterial growth in horse cryopreserved semen","authors":"Diana Fanelli , Rebecca Moroni , Giulia Sala , Pierre Melanie , Ilaria Tarabella , Nicole Telleschi , Saverio Maltinti , Mario Giorgi , Giovanni Barsotti , Fabrizio Passamonti , Paola Marmorini , Alessandra Rota , Francesco Camillo , Duccio Panzani","doi":"10.1016/j.vas.2024.100380","DOIUrl":"10.1016/j.vas.2024.100380","url":null,"abstract":"<div><p>Two studies were conducted to evaluate the use of medical ozone (O<sub>3</sub>) in commercial extenders for equine semen cryopreservation. In the first study (Study 1), 0, 5, and 15 µg/mL of O<sub>3</sub> were added to diluents of refrigerated or frozen semen. Samples were evaluated for sperm kinematics at different time points for the chilled samples and after a thermoresistence test for the frozen/thawed samples. In the second study (Study 2), 0, 5, and 10 µg/mL of O<sub>3</sub> were added to an antibiotic-free diluent for refrigerated semen for comparison with the control group in which semen was diluted in the same diluent enriched with antibiotics. Semen sample kinematics were analyzed and an aliquot was collected after ozonification for bacteriological analyses. For Study 1 no difference was found comparing all the kinematic parameters analyzed over time, in the various treatments (<em>P</em> > 0.05). In Study 2 the absence of antibiotics did not affect the kinematic parameters compared to the control (<em>P</em> > 0.05). However when antibiotics were added, a smaller number of bacterial colony-forming units were detected compared to samples without antibiotics and without or with different O<sub>3</sub> supplementations. In conclusion, O<sub>3</sub> treatment at low dosages did not affect the semen kinematics, although it was ineffective in preventing bacterial overgrowth. Higher O<sub>3</sub> concentrations should be evaluated to explore the possibility of reducing the use of antibiotics in equine sperm conservation.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100380"},"PeriodicalIF":1.9,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000474/pdfft?md5=dacb7e0e0af60e7c91f116a85947073b&pid=1-s2.0-S2451943X24000474-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veronika Vojtkovská, Eva Voslářová, Vladimír Večerek
{"title":"Causes of shelter cats mortality in the Czech Republic","authors":"Veronika Vojtkovská, Eva Voslářová, Vladimír Večerek","doi":"10.1016/j.vas.2024.100379","DOIUrl":"10.1016/j.vas.2024.100379","url":null,"abstract":"<div><p>Awareness of the causes of mortality in shelter cats can contribute to its effective reduction. The aim of this study was to investigate the causes of mortality in cat shelters in the Czech Republic, taking into account the age of the animals and their length of stay in the shelter facilities until natural death or euthanasia. A cooperation with two private no-kill shelters in the Czech Republic was established. The subject of the study were records of 3047 cats admitted to the selected shelters in the period from 2013 to 2023. A total of 357 (11.7 %) out of 3047 cats died or had to be euthanized in two monitored shelters. No significant difference (<em>p</em> > 0.05) in the cat mortality was found between the two shelters (11.5 % and 12 %). The median length of stay of cats in the shelter until death or euthanasia was 23 days. The highest mortality was recorded in the youngest cats (≤ 6 months). The most common causes of death or euthanasia of cats in both shelters were feline infectious peritonitis (21.6 %), panleukopenia (18.5 %) and upper and lower respiratory tract infections (17.5 %). Adult and older cats were significantly (<em>p</em> < 0.05) more prone to death due to renal failure and cancer than young animals and kittens. The results of the study contribute to the knowledge on the health of cat population in shelters in the Czech Republic and can serve as a basis for further work that will target mortality reduction strategies in at-risk categories.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100379"},"PeriodicalIF":1.9,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000462/pdfft?md5=07ecdf18c59a635b34cfbda05a30ad96&pid=1-s2.0-S2451943X24000462-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141732076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vida Juozaitienė, Vesta Jonikė, Dalytė Mardosaitė-Busaitienė, Loreta Griciuvienė, Evelina Kaminskienė, Jana Radzijevskaja, Vilius Venskutonis, Vitas Riškevičius, Algimantas Paulauskas
{"title":"Application of cold plasma therapy for managing subclinical mastitis in cows induced by Streptococcus agalactiae, Streptococcus uberis and Escherichia coli","authors":"Vida Juozaitienė, Vesta Jonikė, Dalytė Mardosaitė-Busaitienė, Loreta Griciuvienė, Evelina Kaminskienė, Jana Radzijevskaja, Vilius Venskutonis, Vitas Riškevičius, Algimantas Paulauskas","doi":"10.1016/j.vas.2024.100378","DOIUrl":"10.1016/j.vas.2024.100378","url":null,"abstract":"<div><p>The primary objective of this study was to assess the effectiveness of cold plasma therapy in managing subclinical mastitis in cows caused by <em>Streptococcus agalactiae, Streptococcus uberis</em> and <em>Escherichia coli</em>. After detection of mastitis pathogens, 38 cows were selected for cold plasma therapy for five days. On the fifth day of treatment, the mastitis agents were re-examined and no causative agents were identified. An additional evaluation conducted 28 days later confirmed the absence of mastitis. Cow productivity, milk composition and quality indicators were assessed at the beginning of the experiment and 32 days from the start (28 days after treatment cessation). After the mastitis treatment, the somatic cell count decreased significantly by between 2.89 and 7.09 times, and the milk yield of the cows at the end of the experiment increased from 0.63 kg per day to 2.82 kg per day (<em>P</em> < 0.01). These results highlight the potential of this innovative approach for managing a prevalent disease that causes substantial losses in the dairy industry. Furthermore, they lay the groundwork for expanded research involving larger sample sizes.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100378"},"PeriodicalIF":1.9,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000450/pdfft?md5=d8d88ba7a74bbfef6f831658074ebc40&pid=1-s2.0-S2451943X24000450-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141706175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of ketoprofen and meloxicam on oxidative stress through the glutathione pathway after ketamine-xylazine anesthesia and ulcer induction in rats: A comparative study","authors":"Azin Sheverini , Ali Khezrian , Ali Shojaeian","doi":"10.1016/j.vas.2024.100377","DOIUrl":"10.1016/j.vas.2024.100377","url":null,"abstract":"<div><p>Given that oxidative stress (OS) occurs as one of the complications of general anesthesia and surgical procedures, practicing the best and safest anesthesia regimen can have a significant share in various surgeries. So, this study compared the effects of non-steroidal anti-inflammatory drugs (NSAIDs) such as ketoprofen (KTP) and meloxicam (MLX) on OS through the glutathione pathway after the ketamine-xylazine (K-X) anesthesia and ulcer induction in rats to suggest post-operative regimens with promising analgesic and anti-inflammatory effects.</p><p>80 healthy adult male Wistar rats, were examined in this study. To obtain the baseline value cardiac blood collected of five rats, and the remaining 75 animals were randomized into three groups of 25, including (i) the control group receiving physiological serum, (ii) the experimental group 1 taking KTP, (iii) the experimental group 2, administered by MLX and all three groups received K-X combination IP after 30 min. Then, a full-thickness ulcer was induced under standard conditions, and the blood samples were collected from groups at T0, T30m, T60m, T24h, and T48h. The serum levels of the desired markers were measured. The study results revealed that the administration of K-X as an anesthetic agent made some changes in the markers of the OS-related glutathione (GSH) pathway. Moreover, KTP and MLX, significantly (<em>p</em> < 0.05) augmented the reduced GSH (rGSH), lowered the GSSG, increased the total values of the glutathione disulfide (GSSG) and the rGSH, reduced the rGSH/GSSG ratio, and accelerated the glutathione peroxidase (GPx) activity, but they had high inhibitory effects on the glutathione reductase (GR). Accordingly, both drugs could maintain the balance between the OS markers, caused by general anesthesia. In general, KTP can be a suitable regimen in surgeries wherein analgesia is of importance for less than 24 h, but MLX can be a preferable option if longer analgesia is needed for more than 24 h.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100377"},"PeriodicalIF":1.9,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000449/pdfft?md5=39119a52d2dc395fbf869e688935ba30&pid=1-s2.0-S2451943X24000449-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141694452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental long bone fracture healing in goats with cockle shell-based calcium carbonate bone paste","authors":"Saroj Kumar Yadav , Subrata Kumar Shil , Monoar Sayeed Pallab , Kh. Nurul Islam , Bibek Chandra Sutradhar , Bhajan Chandra Das","doi":"10.1016/j.vas.2024.100374","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100374","url":null,"abstract":"<div><p>Long bone fractures are common orthopedic conditions. There are numerous ways to repair these fractures. Bone grafting becomes necessary when a broken bone has a significant gap. However, due to insufficient donor volume and donor site morbidity, substitutes are required. In veterinary orthopaedics, calcium carbonate from cockle shells could be used as a bone biomaterial. We investigated its efficacy as a bone biomaterial repair for goat femoral fractures. The study included 10 healthy adult male Black Bengal goats weighing 8 kg and aged 12–13 months. The study includes control and treatment groups. Intramedullary pinning stabilized an 8-mm right femur diaphyseal fracture in the treatment and control groups. The treated group received 2 ml of bone paste in the fractured gap, whereas the control group left it empty. We examined all goats with X-rays on the 7th, 45th, and 60th days, followed by gross and histological findings. Due to callus bridging, radiographs revealed faster bone growth in the treated group than in the control group. Gross examination demonstrates the treated group had a larger fracture callus than the control group. Histopathology showed that bone formed faster and included more osteocytes, osteoblasts, osteoclasts, and bony spicules than in the control group. The treated group had more periosteum osteoblasts, while the control group had fibroblasts. These results showed that the treated group had more osteogenic activity than the control group. This study demonstrates the potential of cockle shell-based calcium carbonate bone paste as a synthetic biomaterial for healing long bone fractures in goats.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100374"},"PeriodicalIF":1.9,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000413/pdfft?md5=5d71e3625728f8b6f82275ebbb82b82b&pid=1-s2.0-S2451943X24000413-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genomic predictions under different genetic architectures are impacted by mating designs","authors":"Sahar Ansari, Navid Ghavi Hossein-Zadeh, Abdol Ahad Shadparvar","doi":"10.1016/j.vas.2024.100373","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100373","url":null,"abstract":"<div><p>Mating in animal communities must be managed in a way that assures the performance increase in the progenies without increasing the rate of inbreeding. It has currently become possible to identify millions of single nucleotide polymorphisms (SNPs), and it is feasible to select animals based on genome-wide marker profiles. This study aimed to evaluate the impact of five mating designs among individuals (random, positive and negative assortative, minimized and maximized inbreeding) on genomic prediction accuracy. The choice of these five particular mating designs provides a thorough analysis of the way genetic diversity, relatedness, inbreeding, and biological conditions influence the accuracy of genomic predictions. Utilizing a stochastic simulation technique, various marker and quantitative trait loci (QTL) densities were taken into account. The heritabilities of a simulated trait were 0.05, 0.30, and 0.60. A validation population that only had genotypic records was taken into consideration, and a reference population that had both genotypic and phenotypic records was considered for every simulation scenario. By measuring the correlation between estimated and true breeding values, the prediction accuracy was calculated. Computing the regression of true genomic breeding value on estimated genomic breeding value allowed for the examination of prediction bias. The scenario with a positive assortative mating design had the highest accuracy of genomic prediction (0.733 ± 0.003 to 0.966 ± 0.001). In a case of negative assortative mating, the genomic evaluation's accuracy was lowest (0.680 ± 0.011 to 0.899 ± 0.003). Applying the positive assortative mating design resulted in the unbiased regression coefficients of true genomic breeding value on estimated genomic breeding value. Based on the current results, it is suggested to implement positive assortative mating in genomic evaluation programs to obtain unbiased genomic predictions with greater accuracy. This study implies that animal breeding programs can improve offspring performance without compromising genetic health by carefully managing mating strategies based on genetic diversity, relatedness, and inbreeding levels. To maximize breeding results and ensure long-term genetic improvement in animal populations, this study highlights the importance of considering different mating designs when evaluating genomic information. When incorporating positive assortative mating or other mating schemes into genomic evaluation programs, it is critical to consider the complex relationship between gene interactions, environmental influences, and genetic drift to ensure the stability and effectiveness of breeding efforts. Further research and comprehensive analyzes are needed to fully understand the impact of these factors and their possible complex interactions on the accuracy of genomic prediction and to develop strategies that optimize breeding outcomes in animal populations.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100373"},"PeriodicalIF":1.9,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000401/pdfft?md5=5ac9870c8f4841a86fe0372a16a6f5ac&pid=1-s2.0-S2451943X24000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Donald Torsabo , Sairatul Dahlianis Ishak , Noordiyana Mat Noordin , Khor Waiho , Ivan Chong Chu Koh , Muhammad Abduh Yazed , Ambok Bolong Abol-Munafi
{"title":"Optimizing reproductive performance in pangasius catfish broodstock: A review of dietary and molecular strategies","authors":"Donald Torsabo , Sairatul Dahlianis Ishak , Noordiyana Mat Noordin , Khor Waiho , Ivan Chong Chu Koh , Muhammad Abduh Yazed , Ambok Bolong Abol-Munafi","doi":"10.1016/j.vas.2024.100375","DOIUrl":"10.1016/j.vas.2024.100375","url":null,"abstract":"<div><p><em>Pangasius</em> catfish, a significant player in the global whitefish market, encounters challenges in aquaculture production sustainability. Quality broodstock maintenance and seed production are impeded by growth, maturation, and fecundity issues. This review investigates the efficacy of strategic nutrient composition and molecular strategies in enhancing broodstock conditions and reproductive performance across various fish species. A notable knowledge gap for <em>Pangasius</em> catfish hampers aquaculture progress. The review assesses nutrient manipulation's impact on reproductive physiology, emphasizing <em>pangasius</em> broodstock. A systematic review analysis following PRISMA guidelines was conducted to identify research trends and hotspots quantitatively, revealing a focus on <em>P. bocourti</em> and fertilization techniques. Addressing this gap, the review offers insights into dietary nutrients manipulation and genetic tool utilization for improved seed production, contributing to <em>pangasius</em> catfish aquaculture sustainability.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100375"},"PeriodicalIF":1.5,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000425/pdfft?md5=71775ea3b185cb45c79b7de4fc4fb1d1&pid=1-s2.0-S2451943X24000425-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141398103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cajanus cajan and Lablab purpureus leaf meal-potential supplements over conventional protein sources for yearling Horro sheep fed a basal diet of fodder oat (Avena sativa) hay","authors":"Abuye Tulu, Worku Temesgen, Tusa Gemechu, Birmeduma Gadisa, Mekonnen Diribsa","doi":"10.1016/j.vas.2024.100376","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100376","url":null,"abstract":"<div><p>A 90-day feeding trial with 25 yearling Horro lambs (22.6 ± 1.8 kg) was conducted from November 2022 to January 2023. The trial was designed to see how supplementing forage varieties of <em>Cajanus cajan</em> (Degagsa and Belabas) and <em>Lablab purpureus</em> (Beresa-55 and Gebis-17) affected the growth performance, feed intake and digestibility of Horro lambs fed a basal diet of fodder oat hay compared to conventional protein supplements. Five experimental sheep per treatment were arranged in a Randomized Complete Blocked Design to receive dietary treatments formulated on an iso-nitrogenous basis which was targeted to provide crude protein (CP) of 64.37 g/h/day. Data were taken on weight gain, feed conversion ratio, feed intake and refusal, and in-vivo digestibility features. All lambs were fed <em>ad-libitum</em> fodder oat hay containing 91.5 % Dry matter, 7.8 % ash, 6.4 % crude protein, 60 % neutral detergent fiber, 42.8 % acid detergent fiber, 13.4 % acid detergent lignin and 65 % in-vitro organic matter digestibility. The dietary treatments were fodder oat hay plus 280.8 g Gebis-17 variety (T1), 274.4 g Beresa-55 variety (T2), 320.9 g Belabas variety (T3), 329.3 g Degagsa variety (T4) and 300 g concentrate mixture (T5). The total dry matter intake (DMI) was higher in T3 and T4 than in the other treatment groups. Except for CP, nutritional consumption differed significantly across treatments. T3 and T4 had higher fiber intakes than the other treatments, while T2 had higher ash intakes and T5 had higher metabolizable energy intakes. Even though there was no significant variation in final body weight, lambs in T5 and T1 showed the greatest weight change and average daily gain. Although the diet in T5 induced the lamb performance in a manner comparable to that of the other dietary supplements, its inaccessibility and high cost would limit the use of such supplements by smallholder farmers. Thus, depending on their availability, either <em>Lablab purpurues</em> or <em>Cajanus cajan</em> varieties could be employed as feed supplements in the lambs` diet.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100376"},"PeriodicalIF":1.9,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000437/pdfft?md5=5df03f52128955ca9a7f7a65f0155e37&pid=1-s2.0-S2451943X24000437-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeiczon Jaimes-Dueñez, Eyner Goyeneche-Ortiz, Marisol Tique-Oviedo, Melissa C Ortiz-Pineda, Luis Cardenas-Pinto, Angela Patricia Jimenez-Leaño, Julian Ruiz-Saenz
{"title":"Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia","authors":"Jeiczon Jaimes-Dueñez, Eyner Goyeneche-Ortiz, Marisol Tique-Oviedo, Melissa C Ortiz-Pineda, Luis Cardenas-Pinto, Angela Patricia Jimenez-Leaño, Julian Ruiz-Saenz","doi":"10.1016/j.vas.2024.100372","DOIUrl":"10.1016/j.vas.2024.100372","url":null,"abstract":"<div><p>Enzootic Bovine Leukosis (EBL), caused by the bovine leukosis virus (BLV), is a global infectious disease affecting livestock. This study focuses on studying the frequency and genetic traits of BLV in three Creole breeds including Chino Santandereano (Chino), Casanareño (CAS), and Sanmartinero (SM) in Eastern Colombia. We implemented a cross-sectional survey between 2019 and 2020 across four departments (Arauca, Casanare, Santander and Meta) in Eastern Colombia to assess the molecular characteristics of BLV infection in these breeds. A total of 253 cattle were analyzed, of which 42.6 %, 28.8 %, and 28.4 % belonged to the Chino, CAS, and SM breeds, respectively. BLV provirus was detected using nested polymerase chain reaction (n-PCR) targeting the conserved region of the <em>env</em> viral gene. Subsequently, the obtained amplicons were sequenced and subjected to phylogenetic analyses. The overall BLV infection frequency was 26.48 % (95 % CI: 21.01 – 31.98 %), with Chino exhibiting the highest frequency (35.1 %) following by SAM and CAS, respectively (<em>P</em> < 0.05). Other epidemiological variables associated with the infection included age, department, and season (<em>P</em> < 0.05). BLV-positive animals exhibited elevated levels of total serum proteins (<em>P</em> < 0.05), while molecular characterization revealed the exclusive circulation of BLV genotype 1 within these breeds. This study provides an updated assessment of BLV infection in Creole breeds from the eastern of Colombia, underscoring their lower infection frequency compared to introduced breeds and their reduced susceptibility to developing clinical signs. The epidemiological and molecular characteristics observed should be considered in developing control programs aimed at improving genetic resistance to BLV in Colombian cattle.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100372"},"PeriodicalIF":1.9,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000395/pdfft?md5=5402d965f37ba4f88cc99592abdbf324&pid=1-s2.0-S2451943X24000395-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141390199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beverly Gartland , William Strunk , Bruce Schulte , Fred DeGraves , Jim Koostra
{"title":"Time budgets differ in horses during continuous and space-restricted rotational grazing","authors":"Beverly Gartland , William Strunk , Bruce Schulte , Fred DeGraves , Jim Koostra","doi":"10.1016/j.vas.2024.100371","DOIUrl":"https://doi.org/10.1016/j.vas.2024.100371","url":null,"abstract":"<div><p>Horses can become obese and develop related health issues such as laminitis from excessive grazing on high-quality pasture grass; limiting pasture intake can allow weight loss to occur. The objective of this study was to determine the effect of space-restricted rotational grazing on body weight (BW) and time budgets in horses. Eight mature geldings and mares with maintenance-only requirements were randomly assigned to either a space-restricted rotational grazing group (SRG; BW 512 ± 6 kg; <em>n</em> = 4) or a continuous grazing group (CG; BW 517 ± 49 kg; <em>n</em> = 4) for 42 d SRG horses grazed an area with dimensions to provide 80–90 % of mean digestible energy requirement for the 4 horses over a 7-d grazing period; whereas, the CG horses continuously grazed similar non-toxic endophyte-infected tall fescue pasture providing greater than maintenance requirements for the 42 d Horses in the SRG group were moved to a new area every 7 d for 6 weeks. On d 7 at 1600 h of each week, horses were brought inside, and feed was withheld overnight. At 0700 h the next day, BWs were recorded prior to turnout. Observers recorded behaviors simultaneously on SRG and CG horses every six minutes throughout the day three days per week according to an ethogram. This included 30 s scans of all horses. Proportion of grazing and standing had an inverse relationship. Proportion of grazing was affected by the treatment by time interaction, which grazing was displayed more in SRG than CG during weeks 2 and 3, and then reversed weeks 4, 5 and 6.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":"25 ","pages":"Article 100371"},"PeriodicalIF":1.5,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000383/pdfft?md5=9b3c1e1e0f031e7264bc5ec0b870edff&pid=1-s2.0-S2451943X24000383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141324437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}