Aquaculture and Fisheries最新文献

{"title":"The isolation and functional identification of a phosphoenolpyruvate carboxykinase gene from Saccharina japonica","authors":"Peichong Lin, Yatong Yao, Lijuan Lu, Yanhui Bi, Zhigang Zhou","doi":"10.1016/j.aaf.2023.08.007","DOIUrl":"https://doi.org/10.1016/j.aaf.2023.08.007","url":null,"abstract":"Similar to other macroalgae, Saccharina japonica has CO2-concentrating mechanism (CCM) that allows high photosynthesis efficiency and elevates biomass. Phosphoenolpyruvate carboxykinase (PEPCK) in cytoplasm is an essential component of CCM. However, no reports on cytosolic PEPCK of S. japonica are available. In this study, the full-length cDNA sequence of a PEPCK gene (SjPCK1) predicted to be localized in cytoplasm, was screened from the full-length transcriptome of S. japonica gametophytes and identified by RT-PCR. The complete cDNA sequence of SjPCK1 was 2174 bp in length, which encompassed an open reading frame (ORF) of 1734 bp, a 5′- untranslated region (UTR) of 216 bp and a 3′-UTR of 224 bp. In parallel, the genomic DNA of SjPCK1 was 21 294 bp in length, characterized by the presence of 11 introns and 12 exons. It encoded a protein of 577 amino acids with a molecular weight of 63 kD and an isoelectric point of 8.47. Amino acid sequence alignment showed that the functional sites of PEPCK were highly conserved in the selected species. Phylogenetic analysis and sequence characterization showed that SjPCK1 was an ATP-dependent PEPCK. SjPCK1 gene was expressed by using Escherichia coli prokaryotic expression system, and the SjPCK1 protein with His 6 tag (rSjPCK1) was 81.93 kD in molecular weight. Enzyme activity assay results showed that the specific activity of carboxylase and decarboxylase of rSjPCK1 was 1.91 ± 0.29 U/mg prog and 11.3 ± 1.97 U/mg prog, respectively. These findings provide valuable molecular and biochemical insights for a further analysis of the role of cytosolic PEPCK in the storage of inorganic carbon in S. japonica.","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of light emitting diode (LED) lamps in the offshore purse seine fishery in Vietnam","authors":"Nguyen Dang Nhat ,&nbsp;Do Thanh Tien ,&nbsp;Truong Van Dan ,&nbsp;Nguyen Duy Quynh Tram ,&nbsp;Nguyen Quang Lich ,&nbsp;Ho Dang Phuc ,&nbsp;Nguyen Ngoc Phuoc","doi":"10.1016/j.aaf.2022.01.005","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.01.005","url":null,"abstract":"<div><p>Fishing with artificial light has become one of the most advanced, efficient, and common methods for the night-time purse seining in Vietnam. This study evaluated the radiation spectrum, CIE chromaticity coordinates, correlated color temperature (CCT), catch rate, fuel consumption, and CO₂ emissions when using Light emitting diode (LED) lamps (0.196 kW) in comparison with the use of metal halide (MH) lights (1 kW) in the offshore purse seine fishery in Quang Tri province, Vietnam. The fishing efficiency of the purse seine fishing boats using LED lamps has increased 1.58 times in catch rate than MH lights, although the energy consumption of LED lamp is 4 times smaller. Fuel consumption of boats per trip using LED lamps was one third of that using MH lights. The use of LED reduced the radiation spectrum, especially the intense UV radiation which negatively affects the health of fishermen. This study also showed the potential of CO₂ emission reduction up to 1.09 tons of CO₂ per trip per boat from the use of LED lamps in the offshore purse seine fishing boats.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 551-557"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of transport stress on immune response, physiological state, and WSSV concentration in the red swamp crayfish Procambarus clarkii","authors":"Ruixue Shi,&nbsp;Siqi Yang,&nbsp;Qishuai Wang,&nbsp;Long Zhang,&nbsp;Yanhe Li","doi":"10.1016/j.aaf.2022.01.001","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.01.001","url":null,"abstract":"<div><p>Transport is an essential part of the aquaculture and research of the main freshwater aquaculture crayfish <em>Procambarus clarkii</em> in China. However, transport is often accompanied by a low survival rate. Assessing the physiological state of <em>P. clarkii</em> before and after transport may discover the cause of this high mortality rate. In this study, ice-cold and exposed-to-air transport methods were compared using an array of parameters, including relative expression level of heat shock protein 70 (<em>HSP70</em>), content of serum glucose and cortisol, immune parameters (enzyme and immune-related genes), and white spot syndrome virus (WSSV) concentration were investigated to understand the physiological state of <em>P. clarkii</em> before and after transport, as well as the cause of dying crayfish on days 5 and 7 after transport stress. Histological sections of hepatopancreas, gills, and intestines reflected pathological changes. The survival rate of crayfish with ice-cold transport was significantly higher than that with exposed-to-air transport, and mortality peaked at 3–9 days after transport stress. A prolonged response to oxidative stress and short-term immunosuppression was present after transport, and the trend of the WSSV concentration in the hepatopancreas was similar to the mortality rate of <em>P. clarkii.</em> The contents of serum glucose and cortisol, antioxidant enzymes and immune-related indexes, and the concentration of WSSV in hepatopancreas of dying crayfish were significantly higher than those of vibrant crayfish on the 5th and 7th days after transport. The hepatopancreas, intestines, and gills of dying crayfish had varying degrees of damage, and the hepatopancreas and intestines were severely damaged. The results suggested that the death of <em>P. clarkii</em> after transport stress is caused by oxidative stress, the imbalance of reactive oxygen species regulation, and decreased WSSV resistance, which eventually led to irreversible tissue damage. The increase of WSSV in the body of crayfish might be the direct cause of crayfish death.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 498-508"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomolecular response of CD4+, CD8+, TNF-α and IFN-γ in Myxobolus-infected koi (Cyprinus carpio) treated with probiotics","authors":"Uun Yanuhar ,&nbsp;Nico Rahman Caesar ,&nbsp;Nur Sakinah Junirahma ,&nbsp;Rachmat Noer Soelistyoadi","doi":"10.1016/j.aaf.2022.01.004","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.01.004","url":null,"abstract":"<div><p>A poor environment increases fish's susceptibility to myxosporean infection that can cause the death of larval fish, especially for koi fish (<em>Cyprinus carpio</em>). This study aimed to determine the effect of probiotics, local anti-parasitic drugs (kutuklin), and the chemical compound diflubenzuron treatments on the koi immune response. This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic. The treatments were divided into 5 groups: Treatment (A) (healthy koi without treatment), (B) (infected koi without treatment), (C) (infected koi with 0.55 mL/30 L probiotics), (D) (infected koi with 1 μL/g of feed kutuklin), and (E) (infected koi with 0.02 mg/5 L dimilin). Myxospore has observed with Scanning Electron Microscopy (SEM) and 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining. The histological analysis using semi-quantitative scoring methods, and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4 (CD4⁺), Cluster of differentiation 8 (CD8⁺), Tumor Necrosis Factor-alpha (TNF-α), Interferon gamma (IFN-γ) cells in the gills. Results show that the histological analysis indicated edema, hyperplasia, lamella fusion, congestion, and hypertrophy lesions in infected koi. Treatment with probiotics shows the lowest damage (30.6%). The immune responses of CD4⁺ and CD8⁺ cells to dimilin treatment were 10.54% and 16.86%, respectively. The largest TNF-α and IFN-γ response were for the kutuklin treatment (29.26%) and probiotics treatment (8.23%).</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 514-527"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49766745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway","authors":"Zhiwen Wang, Ping Deng, Zemao Gu, Lijuan Li, Junfa Yuan","doi":"10.1016/j.aaf.2023.08.004","DOIUrl":"https://doi.org/10.1016/j.aaf.2023.08.004","url":null,"abstract":"Largemouth bass have been plagued by diseases elicited by bacteria, parasites, and viruses, particularly viral diseases, which have caused significant economic losses in recent years. And there is a lack of approved antiviral drugs in the aquaculture industry. Micropterus salmoides Rhabdovirus (MSRV) is the causative agent of Micropterus salmoides Rhabdovirus disease, which mostly affects largemouth bass fry. By using experimental infection, transcriptome sequencing, and inhibitor treatment, the inflammatory response mediated by mitogen-activated protein kinase (MAPK), phosphatidylinositol3-kinase (PI3K), and nuclear factor kappa-B (NF-κB) pathways was verified as one of the pathological processes of MSRV infection. Further, Myricetin, arteannuin, naringenin, ursolic acid, andrographolide, and resveratrol were selected to evolute their anti-inflammatory activity based on the promoter of NF-κB and found myricetin, naringenin, andrographolide, and resveratrol significantly inhibited NF-κB 1 promoter activity. Myricetin was found to damp MSRV caused-inflammation and inhibit MSRV infection by suppressing NF-κB signaling. Besides, myricetin improves the survival rate of largemouth bass after MSRV infection. In conclusion, myricetin presented anti-MSRV activity by targeting the NF-κB inflammatory pathway and can be further developed as a potential preventative drug of rhabdovirus disease.","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Square mesh codend improves size selectivity and catch pattern for Trichiurus lepturus in bottom trawl used along Northwest coast of India","authors":"Madhu Vettiyattil ,&nbsp;Bent Herrmann ,&nbsp;Meenakumari Bharathiamma","doi":"10.1016/j.aaf.2021.12.015","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.12.015","url":null,"abstract":"<div><p>The size selectivity and catch pattern of <em>Trichiurus lepturus</em> in 40 mm diamond and square mesh codends were investigated in this study. A 34 m high opening trawl was used, which is a common design used by fishermen operating along the northwest coast of India, and the cover codend method was employed to determine codend selectivity. Along with the changes in the length at 50% retention rate for <em>Trichiurus lepturus</em> with the usage of the square and diamond mesh codends, indicators to understand the exploitation pattern of this species in the fishery were assessed based on the total number of individuals and on total weight of the catch. The mean selection length increased and discard fraction is reduced when 40 mm square mesh codends are used in place of diamond meshes, however, it is observed that individuals at commercial length are also lost. The results demonstrate that mandatory use of legal mesh sizes alone will be insufficient to aid in the sustainable harvest of this species, given its estimated length at first sexual maturity of 61.2 cm. This is the first study to compare the size selection of this species in 40 mm diamond and square mesh codends along Northwest coast of India. Results of the study will help as a baseline for gear-based regulations in the region.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 564-571"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding previously published articles","authors":"","doi":"10.1016/j.aaf.2022.03.006","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.03.006","url":null,"abstract":"","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 600-601"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii","authors":"Xue Liu ,&nbsp;Lingxia Zhou ,&nbsp;Biyun Luo ,&nbsp;Hongli Qian ,&nbsp;Baoqing Ye ,&nbsp;Keyi Ma ,&nbsp;Gaofeng Qiu","doi":"10.1016/j.aaf.2022.01.003","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.01.003","url":null,"abstract":"<div><p>The giant freshwater prawn (<em>Macrobrachium rosenbergii</em>) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of <em>M. rosenbergii</em>, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of <em>M. rosenbergii</em>.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 509-513"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of gastrin and cholecystokinin B receptor in Lateolabrax maculatus","authors":"Tingwen Cui ,&nbsp;Jiaqi Wang ,&nbsp;Zhongjun Hu ,&nbsp;Xiaowu Chen","doi":"10.1016/j.aaf.2021.11.002","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.11.002","url":null,"abstract":"<div><p>Gastrin (gas) is a peptide hormone that stimulates gastric acid secretion by gastric parietal cells and stimulates gastric motility. The cholecystokinin B receptor (cckbr) can act as a receptor for gastrin, conveying regulatory information on gastrin, but there are fewer studies on its function in fish. The <em>Lateolabrax maculatus</em> is one of the marine aquaculture species in China, it widely distribute in coastal areas. In the study, we cloned the genes of <em>Lateolabrax maculatus</em> gastrin (Lm-gas) and <em>Lateolabrax maculatus</em> cholecystokinin B receptor (Lm-cckbr). The results showed that the full-length gene of Lm-gas is 638bp and the carboxy-terminal conserved domain (DFGRR) is the core functional domain of gastrin protein. The Lm-cckbr gene has a total nucleotide sequence of 2066 bp, and the open reading frame encodes a total of 453 amino acids. The result of protein sequence alignment showed that the similarity between Lm-cckbr protein and other different species was 50.11%–89.67%. The PCR results showed that Lm-gas and Lm-cckbr were expressed in brain and stomach. Further localization by immunehistochemical staining showed that Lm-gas protein was located in the mucosal layer of the gastric wall, but the expression signal was weak in the brain. Hunger causeed a significant decrease in these two genes. The results provided basic research data for further study on the function of Lm-gas and its recepter Lm-cckbr in the in the central nervous system and digestive system of <em>Lateolabrax maculatus</em>.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 492-497"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49766743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting the shelf life of Trachinotus ovatus during frozen storage using a back propagation (BP) neural network model","authors":"Weiqing Lan ,&nbsp;Xin Yang ,&nbsp;Taoshuo Gong ,&nbsp;Jing Xie","doi":"10.1016/j.aaf.2021.12.016","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.12.016","url":null,"abstract":"<div><p>The research aimed to create a shelf life prediction model for <em>Trachinotus ovatus</em> in different freezing temperatures by using back propagation (BP) neural network model. The pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), water retention (water holding capacity [WHC]; cooking loss), and sensory evaluation were measured under 266 K, 255 K, 243 K, 233 K, and 218 K temperatures. The results of TVB-N and water retention during 266 K, 255 K, 233 K, and 218 K conditions were selected to build a BP neural network model and verify the model at 243 K. Results indicated that low temperatures retarded the rise of pH, TVB-N, and TBA values, improving water retention capacity of <em>Trachinotus ovatus</em>. The BP neural network model had high regression coefficients (<em>r</em>²: 0.8642–0.9904), low mean square error (MES: 0.1658–1.7882), and relative error within 10% and could accurately predict the quality change of <em>Trachinotus ovatus</em> under the freezing temperatures of 266 K–218 K. Therefore, (BP) neural network model has great potential in predicting the shelf life of <em>Trachinotus ovatus</em> in frozen storage.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"8 5","pages":"Pages 544-550"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}