Aquaculture and Fisheries最新文献

Dietary administration of probiotic Bacillus coagulans and Mentha piperita can protect histological architecture and DNA damage in Catla catla (Hamilton, 1822) 膳食中添加益生菌凝结芽孢杆菌和薄荷可保护猫鼬(Hamilton，1822)的组织结构和DNA损伤

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.02.002

{"title":"Dietary administration of probiotic Bacillus coagulans and Mentha piperita can protect histological architecture and DNA damage in Catla catla (Hamilton, 1822)","authors":"","doi":"10.1016/j.aaf.2023.02.002","DOIUrl":"10.1016/j.aaf.2023.02.002","url":null,"abstract":"<div><div>The present experimental investigation was executed to evaluate the combined effect of probiotic bacterium <em>Bacillus coagulans</em> along with herb <em>Mentha piperita</em> on histological architecture; and extent of DNA damage after challenge trial with pathogenic bacterium <em>Aeromonas hydrophila</em> in <em>Catla catla</em>. In this experiment, six dietary treatments in triplicate were formulated; C0, M3 and M6 in which <em>M. piperita</em> was supplemented at 0, 3, 6 g kg<sup>−1</sup> of feed, while in dietary treatments CP, MP3 and MP6; supplementation of probiotic bacterium <em>B. coagulans</em> at the rate of 3000 CFU g<sup>−1</sup> was done along with different inclusion level of <em>M. piperita</em> (0, 3, 6 g kg<sup>−1</sup> of feed). Fishes were fed for 60 days on different dietary treatments. Growth performance, histological study and extent of DNA damage using comet assay was examined. Significantly (<em>p</em> < 0.05) high growth % gain in BW was observed in treatment MP6 followed by treatment M6. The histological analysis clearly revealed that dietary <em>M. piperita</em> without or with probiotic bacterium can protect the histoarchitecture of intestinal layers and liver even after challenge with <em>A. hydrophila</em>. Analysis of various comet parameters (tail length, olive moment, tail DNA, head DNA) affirmed that <em>M. piperita</em> supplemented diet can reduce the extent of DNA damage in fishes after challenge trial with <em>A. hydrophila</em>, however dietary supplementation of probiotic bacterium along with <em>M. piperita</em> can reduce the DNA damage up to larger extent confirming its combined protective effect of supplemented diets.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 948-956"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86798169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated mutation of Mstn confers growth performance in Culter alburnus juveniles CRISPR/Cas9 介导的 Mstn 基因突变可提高白腹角雉幼体的生长性能

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.04.008

{"title":"CRISPR/Cas9-mediated mutation of Mstn confers growth performance in Culter alburnus juveniles","authors":"","doi":"10.1016/j.aaf.2023.04.008","DOIUrl":"10.1016/j.aaf.2023.04.008","url":null,"abstract":"<div><div>Myostatin is a member of the TGF-β superfamily and functions as a negative regulator for skeletal muscle development and growth. It has become the most targeted gene in aquaculture that used for selective breeding. Previous studies involved in genome editing in several fish species confirmed that CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) system was highly efficient with lower off-target effect, however, no reports were raised in <em>Culter alburnus</em>. In this study, we employed CRISPR/Cas9 gene editing system to successfully disrupt <em>mstn</em> gene by co-injection with Cas9 protein and the targeted sgRNA in <em>C. alburnus</em>. Various Indel mutations were obtained with 82% knockout efficiency in the F0 generation by PCR sequencing. In addition, mutations in <em>mstn</em> that induced by CRISPR/Cas9 were detected in the F1 generation by individually mating the wild-type female with the F0 generation of <em>mstn</em>-KO male at sexual maturity. More importantly, the body weight and length were significantly elevated in <em>mstn</em> <sup><em>±</em></sup> group when compared to those of the control. As expected in <em>mstn</em> <sup><em>±</em></sup> group, the expression level of <em>mstn</em> was sharply reduced, whereas a slight increase was observed in two growth-related genes (<em>myod</em> and <em>myog</em>). Moreover, higher numbers of muscle fibers were observed in <em>mstn</em> <sup><em>±</em></sup> group, meaning that growth performance in <em>mstn</em> <sup><em>±</em></sup> individuals might be represented by increasing the number of muscle fibers. Taken together, our current study successfully obtained a site-specific modification of <em>mstn</em> using CRISPR/Cas9 technology, and these results provided a new insight for facilitating topmouth culter genetic studies and breeding.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 900-907"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75635969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simvastatin enhanced growth and altered testis transcriptome profiles of male tilapias 辛伐他汀促进雄性罗非鱼的生长并改变其睾丸转录组图谱

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.05.010

引用次数: 0

Stability analysis of reef fish communities based on symbiotic graph model 基于共生图模型的珊瑚礁鱼类群落稳定性分析

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.03.005

{"title":"Stability analysis of reef fish communities based on symbiotic graph model","authors":"","doi":"10.1016/j.aaf.2023.03.005","DOIUrl":"10.1016/j.aaf.2023.03.005","url":null,"abstract":"<div><div>The community stability of coral reefs and fish is the focus of ecological monitoring of coral reefs. Among them, the realization of effective metrics of variations in reef fish communities (i.e., the combined communities of coral reefs and fish) is important for analyzing the stability of communities as well as maintaining the ecological balance of coral reefs. Based on coral reef and fish data collected at St. John's Island from 2004 to 2010, this study proposes a symbiotic graph modeling method to express the biological relationships of reef fish communities, and a Pyramid Match graph kernel method for fusing Attributes (PMA) to quantify community fluctuations to measure interannual variability of communities. The results showed that the community similarity was low in 2006, 2007, and 2008. The total coral cover rate in the study area decreased by 32.04% from 2006 to 2007 and increased by 24% in 2008. The total number of fish fell from 3780 in 2006 to 2596 in 2007 and rose to 6249 in 2008. Among them, the proportion of herbivorous fish decreased to 30.84% in 2007. Furthermore, we have combined the Louvain algorithm with the proposed PMA method to effectively identify the regions that should be prioritized for protection. Experiments were conducted on real datasets with good results, demonstrating the potential of the proposed method to assist in the analysis of community stability and identification of priority conservation areas.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 1007-1017"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72851961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of biological flocculation technology (BFT) on water quality dynamics and immune response of grass carp (Ctenopharyngodon idella) 生物絮凝技术（BFT）对草鱼水质动态和免疫反应的影响

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.04.005

{"title":"Effects of biological flocculation technology (BFT) on water quality dynamics and immune response of grass carp (Ctenopharyngodon idella)","authors":"","doi":"10.1016/j.aaf.2023.04.005","DOIUrl":"10.1016/j.aaf.2023.04.005","url":null,"abstract":"<div><div>The biofloc technology (BFT) offers benefits in improving feed utilization and reducing the feed conversion ratio compared to the recirculating aquaculture system (RAS). In this study, high-throughput 16S rRNA gene sequencing was employed to investigate and compare the bacterial communities in these aquaculture systems and the gut microbiota of grass carp reared in them. We observed a significant distinction between the microbial communities of the aquaculture systems and the intestinal microbiota of grass carp. The genera <em>cetobacterium</em> and <em>bacillus</em> were more abundant in the BFT system, accounting for only 0.05% and 0.25% in the RAS. Our study also demonstrated that BFT could influence the intestinal microorganisms of grass carp (<em>Ctenopharyngodon Idella</em>) by reducing the relative abundance of <em>burkholderia</em>-<em>caballeronia</em>-<em>paraburkholderia</em> and increasing that of <em>cetobacterium</em>. Based on bugBase community predictions, the potential pathogenicity in BFT and grass carp intestine was lower than in RAS. Regarding gene expression in the grass carp intestine, immunosuppressive genes showed higher expression, while pro-inflammatory genes exhibited lower expression in grass carp reared in BFT. Furthermore, the final average length and weight of grass carp in the BFT system were significantly higher than those in the RAS. This study provides valuable insights into gut microbiome ecology in relation to two grass carp aquaculture systems, which could be effectively applied in grass carp management to promote health and improve water quality.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 921-928"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90102894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of microbe-derived antioxidants on growth, digestive and aminotransferase activities, and antioxidant capacities in the hepatopancreas of Eriocheir sinensis under ammonia nitrogen stress 微生物源抗氧化剂对氨氮胁迫下中华鳖肝胰腺的生长、消化酶和转氨酶活性以及抗氧化能力的影响

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2022.12.002

{"title":"Effects of microbe-derived antioxidants on growth, digestive and aminotransferase activities, and antioxidant capacities in the hepatopancreas of Eriocheir sinensis under ammonia nitrogen stress","authors":"","doi":"10.1016/j.aaf.2022.12.002","DOIUrl":"10.1016/j.aaf.2022.12.002","url":null,"abstract":"<div><div>This study evaluated the effects of feeding a microbial-derived antioxidant diet (MA) for 56 days on the growth performance of Eriocheir sinensis, as well as on digestive and metabolic enzyme activity and antioxidant capacity under ammonia stress (400 mg/L ammonia nitrogen for 48 h). The weight gain at 28d and the specific growth rate at 56ds for crabs in the MA group were significantly higher than those in the control diet (C) group (<em>p</em> < 0.05). Compared with the C group, the color of the hepatopancreas in the MA group was significantly improved (<em>p</em> < 0.05). A significant decrease in the level of Hemolymph ammonia was found in the microbial antioxidant ammonia-N (MA_A) group (<em>p</em> < 0.05), which may be related to the decrease of transaminase (AST, ALT, GDH) activities caused by MA diet (<em>p</em> < 0.05). There is a significant increase in the digestive enzyme activities (Trypsin and Lipase), which reduced the accumulation of triglycerides in the hepatopancreas in the (MA_A) group (<em>p</em> < 0.05). In addition, the antioxidant damage of crabs in hepatopancreas caused by ammonia nitrogen toxicity could also significantly be improved by MA fed in the (MA_A) group (<em>p</em> < 0.05). microbe-derived antioxidants (supplemented with 0.2% in feeds) can protect the health of hepatopancreas, promote growth, and improve the ability to resist ammonia nitrogen stress for E.sinensis.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 957-966"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73160500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IKKε positively regulates NF-κB, MAPK, and IRF3-mediated type I IFN signaling pathways in Japanese eel (Anguilla japonica) IKKε 正向调节日本鳗鲡体内 NF-κB、MAPK 和 IRF3 介导的 IFN 信号通路

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.01.001

{"title":"IKKε positively regulates NF-κB, MAPK, and IRF3-mediated type I IFN signaling pathways in Japanese eel (Anguilla japonica)","authors":"","doi":"10.1016/j.aaf.2023.01.001","DOIUrl":"10.1016/j.aaf.2023.01.001","url":null,"abstract":"<div><div>IKKε is an IκB kinase participating in the control of NF-κB and type I IFN signal pathways in mammals. However, the function of IKKε in regulating immune response is largely unknown in teleost. Herein, an IKKε homologue named <em>Aj</em>IKKε was characterized in Japanese eel (<em>Anguilla japonica</em>). <em>Aj</em>IKKε has an N-terminal kinase domain, a ubiquitin-like domain, and a coiled coil-containing domain (CC), which is conserved and similar to its counterpart in mammals. Expression analysis showed that <em>Aj</em>IKKε could be up-regulated in kidney, spleen, and particularly in liver under the stimulation of poly I:C, LPS, and <em>Aeromonas hydrophila</em> infection. <em>In vitro</em>, the mRNA levels of <em>Aj</em>IKKε were significantly provoked in eel liver cells stimulated by LPS and poly I:C, or the different concentrations of <em>A. hydrophila</em>. The overexpression of <em>Aj</em>IKKε could not only induce a significantly higher level of promoter activity of human NF-κB, AP-1, and IFN-β in a dose-dependent manner but also up-regulate the activation of promoters of Japanese eel cRel, AP1, IL6, IFN4, IRF3, and IRF7 in HEK293 cells. RNAi studies showed that after <em>Aj</em>IKKε was knocked down, the expression levels of IL1, IL6, TNFα, c-Jun, IFN2, IFN3, MX1, MX2, and IRF3 genes were significantly down-regulated in liver, spleen, and kidney of Japanese eels. In addition, the mutants of <em>Aj</em>IKKε-K39A, <em>Aj</em>IKKε-S174A, and <em>Aj</em>IKKε-ΔCC failed to activate Japanese eel IFN4, IRF3 and human IFN-β promoters in HEK293 cells. Collectively, these results suggest that <em>Aj</em>IKKε may function as a positive regulator of NF-κB, MAPK, and IRF3-mediated type I IFN signaling pathways related to immune response evoked by bacterial and viral infection.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 908-920"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78132520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A compact underwater stereo vision system for measuring fish 用于测量鱼类的紧凑型水下立体视觉系统

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.03.006

{"title":"A compact underwater stereo vision system for measuring fish","authors":"","doi":"10.1016/j.aaf.2023.03.006","DOIUrl":"10.1016/j.aaf.2023.03.006","url":null,"abstract":"<div><div>In the context of aquaculture, fish length is a key parameter to assess fish stocks, essential for feeding regime and contributes for decisions at several production levels. However, taking length measurements is a cumbersome task that, when applied to living individuals, can induce considerable stress, increasing the risk of damage or hindering their growth. Computer vision is one of the most used non-contact tools to overcome this issue, being fast, consistent and repeatable. However, its use in aquatic environments is limited by the high cost, the difficulty of calibrating the system in underwater conditions and the complexity of implementation. This paper proposes a low-cost easy-to-use vision system that can take measurements on live fish in aquatic conditions, without the need for a special calibration or a demanding in-water calibration service. The present work implemented a compact stereo vision system and developed a method that estimates the correct length of fish, based on the variation of the angle of incidence of the light rays in the water. Given some structural conditions such as a short baseline, the system is able to measure fish with an error of less than 1%. The short baseline allows to have a compact system and reduces the effect of water refraction on the 3D reconstruction. A set of experiments were performed with real fish, working robustly for a set of orientations of the fish (even when the caudal fin and snout are on different distances to the cameras).</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 1000-1006"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75300570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sturgeon and paddlefish: Review of research on broodstock and early life stage management 鲟鱼和桨鱼：鱼种和早期生命阶段管理研究综述

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.04.001

{"title":"Sturgeon and paddlefish: Review of research on broodstock and early life stage management","authors":"","doi":"10.1016/j.aaf.2023.04.001","DOIUrl":"10.1016/j.aaf.2023.04.001","url":null,"abstract":"<div><div>Sturgeon aquaculture has taken a parallel yet unique path relative to other teleost rearing. One of the driving factors has been the fact that virtually all extant species are facing extinction; thus, laws concerning the transport and processing of sturgeon are stringent. As a result, most sturgeon companies vertically integrate the farming process from hatchery to harvest, in contrast to the production of other commercial fish species (e.g., trout, sea bass, salmon), where farmers tend to purchase fry from producers specialized only in the production of that life stage and sell product to unaffiliated fish processors. Sturgeon aquaculture is furthermore unique in the extremely high value of the main product (caviar). The secondary product (meat) also surpasses other finfish aquaculture products' average price per weight. These expensive outputs reveal the greatest challenge in production — an exceptionally long time to market from larvae to harvestable adults. For all their rigor and robustness as juveniles and adults, sturgeon species are highly fragile during larval stages. Due to the unique legal framework around sturgeon cultivation, aquaculturists often specialize in regional species, although some hybrids have achieved global popularity. This lends to a heterogenous swath of literature on best cultivation and management practices, especially at early growth stages. Nonetheless, the role of aquaculture as both a food source and restocking program to thwart further extinction of sturgeon species has hardly been fully tapped. In light of the many challenges, this review examines recent advances in sturgeon farming with an emphasis on broodstock and early life-stage management to summarize studies that can help researchers and farmers assess the industry's current state.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 871-882"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82357053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of structural parameters on the hydrodynamic performance of vertical curved V-Type otter board 结构参数对垂直曲面 V 型獭兔板水动力性能的影响

Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.02.004

{"title":"Effect of structural parameters on the hydrodynamic performance of vertical curved V-Type otter board","authors":"","doi":"10.1016/j.aaf.2023.02.004","DOIUrl":"10.1016/j.aaf.2023.02.004","url":null,"abstract":"<div><div>In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board, this paper based on the computational fluid dynamics method, a three-dimensional numerical model of vertical curved V-type otter board is established to calculate and analyze its hydrodynamic performance, and the validity of the numerical simulation results is verified by designing and constructing a scaled model for dynamic flume experiments. On this basis, the effects of different structural parameters, such as the deflector angle, camber, and spacing, on the hydrodynamic performance of the vertical curved V-Type otter board are investigated and the optimal structural design scheme is determined. The results demonstrate that single or synchronous changes of the angle, camber, and spacing of the two deflectors have varying degrees of impact on the hydrodynamic performance of the vertical curved V-Type otter board. To improve the expansion effect of the otter board, setting the angle of the deflector A to 40° and that of the deflector B to 30°, or setting the camber of deflectors A and B to 18%, or setting the spacing between deflectors A and B to 380 mm and that between the deflector and the main panel to 670 mm can increase the lift coefficient of the vertical curved V-Type otter board to its maximum value. Considering the overall performance of the otter board, when the angle of the deflector A is set to 25° and that of the deflector B is set to 30°, or the camber of the deflector A is set to 6% and that of the deflector B is set to 9%, or the spacing between deflectors A and B is set to 340 mm and that between the deflector and the main panel is set to 610 mm, the lift-to-drag ratio of the otter board can be increased to its maximum value.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 989-999"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90304361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0