Aquaculture and Fisheries最新文献

{"title":"A new crustin-like gene from the redclaw crayfish Cherax quadricarinatus and its response to decapod iridescent virus 1 (DIV1) challenge","authors":"Xiaoying Hang,&nbsp;Jianbo Zheng,&nbsp;Fei Li","doi":"10.1016/j.aaf.2023.07.005","DOIUrl":"10.1016/j.aaf.2023.07.005","url":null,"abstract":"<div><div>Crustin, a widely-studied antimicrobial peptides (AMPs), has been proven to be involved in mediating innate immune responses against intruding pathogens. In this study, a novel <em>crustin</em>-like gene, <em>Cqcarc1</em>, was characterized from the redclaw crayfish <em>Cherax quadricarinatus.</em> The full-length cDNA sequence was 488 bp, and the open reading frame encoded 101 amino acids with a signal peptide in the N-terminus and a conserved WAP domain in the C-terminus. Similar to the <em>Crustin2</em> gene (<em>CqCrs</em>) in <em>C. quadricarinatus</em>, the highest expression level of <em>Cqcarc1</em> was also detected in the hemocyte organ. Moreover, RNAi-mediated gene silencing of <em>Cqcarc1</em> could increase the DIV1 copy number compared with the control group, suggesting its involvement in the immune defense against the DIV1 challenge. Furthermore, RNA interference analysis also showed that <em>Cqcarc1</em> plays positive regulatory roles in the expressions of <em>CqToll</em> and <em>CqCrs</em> during DIV infection. In summary, the current findings suggested the Toll-crustin signal pathway as a potential target for improving immunity from the DIV1 challenge.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 49-54"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75637870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of new varieties adoption behavior of Chinese turbot farmer based on the perspective of heterogeneity","authors":"Qilei Ding ,&nbsp;Zhengyong Yang ,&nbsp;Renjie Xu ,&nbsp;Yifei Chen","doi":"10.1016/j.aaf.2023.07.001","DOIUrl":"10.1016/j.aaf.2023.07.001","url":null,"abstract":"<div><div>China is the largest turbot farming in the world, where germplasm source security is closely related to national security. To further promote the sustainable development of the turbot aquaculture industry, the China Marine Fish Industry Technology System team has developed a new variety of fast-growing turbot (hereinafter referred to as new varieties) in 2012. However, the promotion effect of new varieties has not reached the expectation, requiring research on the adoption behavior analysis of new varieties. Based on 272 turbot farmers, this study conducts theoretical analysis of framework of new varieties adoption, uses the binary Logit regression model to analyze the determinants of new varieties adoption behavior based on the perspective of heterogeneity. Heterogeneity means that farmers have certain differences, including differences in age, farming scale and enterprise form. Findings indicated that the adoption behavior of new varieties was affected by farming scale, education level, farming years, risk preference, channels of obtaining information, training effect, and government incentives. The adoption behavior of new varieties is group heterogeneity. The causes of low adoption rate of new varieties are farmer's lower risk preference, lower benefit of communication and limited government incentives. The results indicated that new varieties are more likely to be adopted in enterprises, young adult, and large-scale farmers groups. Policy implications from this research included strengthen government incentives for the promotion of new varieties, constructing the systematic and long-term training system and constructing the farmers-centered promotion system.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 163-170"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85465819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and behavioral studies reveal that glutamate receptors are involved in depressive-like behavior in adult olive flounder Paralichthys olivaceus","authors":"Liangfang Liu ,&nbsp;Tangsong Feng ,&nbsp;Mengmeng Shi ,&nbsp;Huafeng Zou ,&nbsp;Weiqun Lu","doi":"10.1016/j.aaf.2023.08.002","DOIUrl":"10.1016/j.aaf.2023.08.002","url":null,"abstract":"<div><div>Olive flounder (<em>Paralichthys olivaceus</em>), a marine demersal flatfish species, exhibits obvious timid and depressive-like characteristics. Adult olive flounder bury themselves in the sand and present motionless behavior. However, the detail mechanism of regulating the motionless behavior is not fully understood. In this study, using adult group (AG) and juvenile group (JG) flounder as a model, we compared the locomotor activity of AG and JG flounder, and analyzed the transcriptomic expression profiles in the spinal cord by RNA-seq methods. The behavioral experiment showed that AG flounder displayed lower locomotor activity. Transcriptome analysis found a total of 1711 significantly differential expressed genes (DEGs), among which 818 genes were up-regulated and 893 genes were down-regulated in AG flounder compared with JG flounder. Specifically, locomotor behavior related genes (<em>parvalbumin</em>, <em>urotensin II</em> and <em>creatine kinase</em>) were down-regulated. Meanwhile glial fibrillary acidic protein, the biomarkers of astroglial pathology, and glutamatergic synaptic genes were significantly up-regulated in AG flounder. In addition, Gene Ontology (GO) enrichment analysis revealed that DEGs enriched in peptide biosynthetic process, translation procession were down-regulated in the AG flounder. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that ribosome, oxidative phosphorylation and glutamatergic synapse pathway were over-represented. After treatment with antagonist of glutamate receptor LY341495, flounder more often swam out of the sand and displayed significantly more off-bottom swimming behavior, suggesting the anti-depressive effect of LY341495. Taken together, in combination with behavioral tests and transcriptomic analyses, we conclude that the glutamate receptor in spinal cord plays an important role in timid and depressive-like behavior in flounder.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 115-123"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90673099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis","authors":"Jirakanit Chimnual ,&nbsp;Jirawat Saetan ,&nbsp;Wilaiwan Chotigeat","doi":"10.1016/j.aaf.2023.06.003","DOIUrl":"10.1016/j.aaf.2023.06.003","url":null,"abstract":"<div><div>The genetic diversity of the banana shrimp <em>Fenneropenaeus merguiensis</em> is at risk because of over-harvesting, which consequently reduces food security. This endangerment is exacerbated because this species is not commonly cultivated by farmers. Overall, these factors necessitate conservation of this shrimp species across its natural habitat. Information on the migration of primordial germ cells (PGCs) to form a gonad is essential for shrimp preservation techniques such as broodstock preparation, sex differentiation, and germ cell transplantation. In this study, histological analysis and <em>in situ</em> hybridization of <em>vasa</em> expression (from embryo to testis development) were used to demonstrate the movement of PGCs. Hematoxylin and Eosin staining and <em>in situ</em> hybridization with the VASA probe revealed that the PGCs migrated retrogradely along the midgut, colonizing the area between the hepatopancreas and heart, a region that becomes the genital ridge in the postlarval stage. External sexual organs appeared at approximately 4 months of age. Through real-time PCR, the expression of the <em>vasa</em> gene was detected early on postlarval day 7, whereas its abundant expression was detected in the ovaries and testes of adult shrimp. This study could help with the identification and monitoring of PGCs or spermatogonia in banana shrimp and facilitate the implementation of other germ cell-relevant techniques in the future.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 94-104"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84587990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of selenium nanoparticles extracted from Bacillus subtilis and its antibacterial effects in Onychostoma macrolepis: Insight into ferroptosis","authors":"Chao Zhu ,&nbsp;Guofan Peng ,&nbsp;Jincan Li ,&nbsp;Yalong Deng ,&nbsp;Tianqi Jin ,&nbsp;Qimin Liu ,&nbsp;Heran Cao ,&nbsp;Zifang Wu ,&nbsp;Yuxuan Ma ,&nbsp;Zhenpeng Li ,&nbsp;Yang Wang ,&nbsp;Lijuan Zheng ,&nbsp;Xiangtong Yuan ,&nbsp;Lijun Zhang ,&nbsp;Fangxia Yang ,&nbsp;Hong Ji ,&nbsp;Wuzi Dong","doi":"10.1016/j.aaf.2023.05.008","DOIUrl":"10.1016/j.aaf.2023.05.008","url":null,"abstract":"<div><div>Selenium nanoparticles (SeNPs) were often used as growth and antioxidant capacity promoter. In this study, we found selenium nanoparticles (SeNPs) could improve the survival rate of <em>Onychostoma macrolepis</em> (<em>O. macrolepis</em>) fry as a feed additive after a challenged by <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>). To further explore the mechanism of SeNPs against bacterial infection, we examined the changes before and after bacterial infection, and the results showed that large amounts of peroxide were produced under <em>A. hydrophila</em> infection in <em>O. macrolepis</em> fry, and then the excessive accumulation of peroxides led to a large amount of ferroptosis in the liver. Interestingly, we found supplementing 0.9 mg/kg biogenic SeNPs decreased MDA, iron content, and enhanced T-AOC levels in the liver of <em>O. macrolepis</em> fry after a challenged by <em>Aeromonas hydrophila</em> (P˂0.01). Especially, the diet with 0.9 mg/kg biogenic SeNPs could significantly increase the expression level of Glutathione peroxidase-4 (GPX4), nuclear factor erythroid2-related factor 2 (Nrf2), and Ferroportin 1 (FPN1) in the liver of <em>O. macrolepis</em> after infection with <em>A. hydrophila</em>. In summary, the results of this research suggested that the diet with 0.9 mg/kg biogenic SeNPs was an optimal additive dose to improve the survival rate of <em>O. macrolepis</em> fry by increasing antioxidant capacity and inhibiting ferroptosis, which sheds a new light on the mechanism of antibacterial infection in fish from the perspective of ferroptosis.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 39-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75975606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress","authors":"Xiao Wu ,&nbsp;Yaoran Fan ,&nbsp;Jianbin Feng ,&nbsp;Keyi Ma ,&nbsp;Jiale Li","doi":"10.1016/j.aaf.2023.05.007","DOIUrl":"10.1016/j.aaf.2023.05.007","url":null,"abstract":"<div><div>Temperature is an essential environmental factor affecting the viability of organisms. Temperature changes could cause damages or even death. The oriental river prawn, <em>Macrobrachium nipponense</em>, is widely distributed in East Asian countries, and has been an important economical aquaculture species in China since 1990s. This prawn belongs to crustaceans, which is a kind of ectotherms and very sensitive to temperature changes due to their relatively low internal metabolic heat production and their high thermal conductivity. In order to reveal the thermal adaptation mechanisms, <em>M. nipponense</em> were acclimated at 25 °C for 24 h, and successively challenged at 30 °C and 35 °C for 24 h in the same tank, respectively. And histological, biochemical and transcriptomic analyses were carried out on the gills and hepatopancreas under different temperatures. The results showed that different degrees of histological damages were discovered both in the gills and hepatopancreas of <em>M. nipponense</em> at 30 °C and 35 °C. The digestive, metabolic, and antioxidative capacity were enhanced in the gills and hepatopancreas of <em>M. nipponense</em> at 30 °C and 35 °C. A total of 2293 and 3304 differentially expressed genes (DEGs) were identified from the gills and hepatopancreas, respectively. These DEGs were significantly involved in the regulation of transcription, proteolysis, nucleus, cytoplasm, metal ion binding, and ATP binding by Gene ontology (GO) enrichment analysis. Furthermore, the DEGs in the hepatopancreas were significantly enriched in KEGG pathways including neuroactive ligand-receptor interaction, thyroid hormone synthesis, and ECM-receptor interaction. And the DEGs in the gills were enriched in KEGG pathways including cGMP-PKG signaling pathway, ribosome, and calcium signaling pathway. These results could be helpful for further understanding the molecular mechanisms underlying the thermal response of <em>M. nipponense</em>, and increasing knowledge of the regulation mechanisms of freshwater crustaceans in the context of global warming.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 27-38"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86096103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chrysene alters the expression pattern of HSP70 genes in mandarin fish","authors":"Yufei Liu ,&nbsp;Tiantian Chen ,&nbsp;Jinliang Zhao ,&nbsp;Xiaowu Chen","doi":"10.1016/j.aaf.2023.07.007","DOIUrl":"10.1016/j.aaf.2023.07.007","url":null,"abstract":"<div><div>The heat shock protein 70 (HSP70) in fish plays a crucial role in various physiological and environmental stresses. To reveal the mechanisms of the <em>HSP70</em> gene response to abiotic stress in mandarin fish (<em>Siniperca chuatsi</em>), we analyzed the HSP70 gene family in this species. There are 16 <em>HSP70</em> genes in mandarin fish, and their protein three-dimensional structures were constructed. The aliphatic index of the 16 mandarin fish HSP70sranges from 73.50 (HSPa4b) to 99.46 (HSPa13), and all are hydrophilic proteins, with seven of them being unstable proteins. After exposure to chrysene, six <em>HSP70</em> genes in the intestine, four in the liver, and four in the brain tissues exhibited significant expression changes. These findings reveal that chrysene exposure affects the physiological status of fish, and <em>HSP70</em> genes may play a role in the chrysene exposure tolerance of the intestine, brain and liver in mandarin fish.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 63-67"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88076478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The involvement of reactive oxygen species in wounding-induced archeospores production in Neopyropia yezoensis","authors":"Dahai Gao ,&nbsp;Chaofeng Teng ,&nbsp;Xinghong Yan","doi":"10.1016/j.aaf.2023.06.002","DOIUrl":"10.1016/j.aaf.2023.06.002","url":null,"abstract":"<div><div>The archeospores released from the blades of <em>Neopyropia yezoensis</em> via dedifferentiated vegetative cells have drawn attention both in cultivation and research. Wounding-induced archeospores formation and release have been observed in <em>N. yezoensis</em>, but the mechanism behind them is unclear. In this study, the involvement of reactive oxygen species (ROS) in the process of wounding-induced archeospores formation and release was investigated. Based on ROS fluorescence observations, the blade fragments began to accumulate ROS after 12 h wounding, while no ROS signals were observed in normal blades. Next, when the blade fragments were treated with ROS inhibitor diphenyleneiodonium (DPI), it showed that the production of archeopores was significantly suppressed. Under normal culturing after wounding, 78.3% of the fragments could release archeospores, and the ROS fluorescence was enriched in the released archeospores. Under 0.05 μM DPI treatment, the percentage of fragments releasing archeospores was dropped to 16.2%, with decreased ROS fluorescence levels. Under 0.1 μM DPI treatment, no archeospores were released from the fragments, and ROS fluorescence was also undetectable in the fragments. Our findings proved that ROS are essential for wounding-induced archeospores production, which might play regulatory roles in the cell dedifferentiation of <em>N. yezoensis</em>.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 1","pages":"Pages 89-93"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82577788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary administration of probiotic Bacillus coagulans and Mentha piperita can protect histological architecture and DNA damage in Catla catla (Hamilton, 1822)","authors":"","doi":"10.1016/j.aaf.2023.02.002","DOIUrl":"10.1016/j.aaf.2023.02.002","url":null,"abstract":"<div><div>The present experimental investigation was executed to evaluate the combined effect of probiotic bacterium <em>Bacillus coagulans</em> along with herb <em>Mentha piperita</em> on histological architecture; and extent of DNA damage after challenge trial with pathogenic bacterium <em>Aeromonas hydrophila</em> in <em>Catla catla</em>. In this experiment, six dietary treatments in triplicate were formulated; C0, M3 and M6 in which <em>M. piperita</em> was supplemented at 0, 3, 6 g kg⁻¹ of feed, while in dietary treatments CP, MP3 and MP6; supplementation of probiotic bacterium <em>B. coagulans</em> at the rate of 3000 CFU g⁻¹ was done along with different inclusion level of <em>M. piperita</em> (0, 3, 6 g kg⁻¹ of feed). Fishes were fed for 60 days on different dietary treatments. Growth performance, histological study and extent of DNA damage using comet assay was examined. Significantly (<em>p</em> &lt; 0.05) high growth % gain in BW was observed in treatment MP6 followed by treatment M6. The histological analysis clearly revealed that dietary <em>M. piperita</em> without or with probiotic bacterium can protect the histoarchitecture of intestinal layers and liver even after challenge with <em>A. hydrophila</em>. Analysis of various comet parameters (tail length, olive moment, tail DNA, head DNA) affirmed that <em>M. piperita</em> supplemented diet can reduce the extent of DNA damage in fishes after challenge trial with <em>A. hydrophila</em>, however dietary supplementation of probiotic bacterium along with <em>M. piperita</em> can reduce the DNA damage up to larger extent confirming its combined protective effect of supplemented diets.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 948-956"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86798169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated mutation of Mstn confers growth performance in Culter alburnus juveniles","authors":"","doi":"10.1016/j.aaf.2023.04.008","DOIUrl":"10.1016/j.aaf.2023.04.008","url":null,"abstract":"<div><div>Myostatin is a member of the TGF-β superfamily and functions as a negative regulator for skeletal muscle development and growth. It has become the most targeted gene in aquaculture that used for selective breeding. Previous studies involved in genome editing in several fish species confirmed that CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) system was highly efficient with lower off-target effect, however, no reports were raised in <em>Culter alburnus</em>. In this study, we employed CRISPR/Cas9 gene editing system to successfully disrupt <em>mstn</em> gene by co-injection with Cas9 protein and the targeted sgRNA in <em>C. alburnus</em>. Various Indel mutations were obtained with 82% knockout efficiency in the F0 generation by PCR sequencing. In addition, mutations in <em>mstn</em> that induced by CRISPR/Cas9 were detected in the F1 generation by individually mating the wild-type female with the F0 generation of <em>mstn</em>-KO male at sexual maturity. More importantly, the body weight and length were significantly elevated in <em>mstn</em> ^<em>±</em> group when compared to those of the control. As expected in <em>mstn</em> ^<em>±</em> group, the expression level of <em>mstn</em> was sharply reduced, whereas a slight increase was observed in two growth-related genes (<em>myod</em> and <em>myog</em>). Moreover, higher numbers of muscle fibers were observed in <em>mstn</em> ^<em>±</em> group, meaning that growth performance in <em>mstn</em> ^<em>±</em> individuals might be represented by increasing the number of muscle fibers. Taken together, our current study successfully obtained a site-specific modification of <em>mstn</em> using CRISPR/Cas9 technology, and these results provided a new insight for facilitating topmouth culter genetic studies and breeding.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 900-907"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75635969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}