运输胁迫对克氏原螯虾免疫反应、生理状态和WSSV浓度的影响

Q1 Agricultural and Biological Sciences
Ruixue Shi, Siqi Yang, Qishuai Wang, Long Zhang, Yanhe Li
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引用次数: 0

摘要

运输是我国主要淡水养殖克氏原螯虾养殖和研究的重要组成部分。然而,运输往往伴随着低存活率。评估克氏疟原虫运输前后的生理状态可能会发现这种高死亡率的原因。在这项研究中,使用一系列参数对冰冷和暴露于空气运输方法进行了比较,包括热休克蛋白70(HSP70)的相对表达水平、血清葡萄糖和皮质醇含量、免疫参数(酶和免疫相关基因)、,为了了解克氏疟原虫运输前后的生理状态以及运输应激后第5天和第7天小龙虾死亡的原因。肝胰腺、鳃和肠的组织学切片反映了病理变化。冰冷运输小龙虾的存活率显著高于暴露于空气运输的小龙虾,死亡率在运输应激后3–9天达到峰值。运输后对氧化应激和短期免疫抑制的反应延长,肝胰腺中WSSV浓度的趋势与克氏疟原虫的死亡率相似。运输后第5天和第7天,死亡小龙虾的血糖和皮质醇含量、抗氧化酶和免疫相关指标以及肝胰腺WSSV浓度均显著高于活力小龙虾。濒临死亡的小龙虾的肝胰腺、肠道和鳃都有不同程度的损伤,肝胰腺和肠道受损严重。结果表明,克氏疟原虫在运输应激后的死亡是由氧化应激、活性氧调节失衡和WSSV耐药性降低引起的,最终导致不可逆的组织损伤。WSSV在小龙虾体内的增加可能是小龙虾死亡的直接原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of transport stress on immune response, physiological state, and WSSV concentration in the red swamp crayfish Procambarus clarkii

Transport is an essential part of the aquaculture and research of the main freshwater aquaculture crayfish Procambarus clarkii in China. However, transport is often accompanied by a low survival rate. Assessing the physiological state of P. clarkii before and after transport may discover the cause of this high mortality rate. In this study, ice-cold and exposed-to-air transport methods were compared using an array of parameters, including relative expression level of heat shock protein 70 (HSP70), content of serum glucose and cortisol, immune parameters (enzyme and immune-related genes), and white spot syndrome virus (WSSV) concentration were investigated to understand the physiological state of P. clarkii before and after transport, as well as the cause of dying crayfish on days 5 and 7 after transport stress. Histological sections of hepatopancreas, gills, and intestines reflected pathological changes. The survival rate of crayfish with ice-cold transport was significantly higher than that with exposed-to-air transport, and mortality peaked at 3–9 days after transport stress. A prolonged response to oxidative stress and short-term immunosuppression was present after transport, and the trend of the WSSV concentration in the hepatopancreas was similar to the mortality rate of P. clarkii. The contents of serum glucose and cortisol, antioxidant enzymes and immune-related indexes, and the concentration of WSSV in hepatopancreas of dying crayfish were significantly higher than those of vibrant crayfish on the 5th and 7th days after transport. The hepatopancreas, intestines, and gills of dying crayfish had varying degrees of damage, and the hepatopancreas and intestines were severely damaged. The results suggested that the death of P. clarkii after transport stress is caused by oxidative stress, the imbalance of reactive oxygen species regulation, and decreased WSSV resistance, which eventually led to irreversible tissue damage. The increase of WSSV in the body of crayfish might be the direct cause of crayfish death.

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来源期刊
Aquaculture and Fisheries
Aquaculture and Fisheries Agricultural and Biological Sciences-Aquatic Science
CiteScore
7.50
自引率
0.00%
发文量
54
审稿时长
48 days
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