Xue Liu , Lingxia Zhou , Biyun Luo , Hongli Qian , Baoqing Ye , Keyi Ma , Gaofeng Qiu
{"title":"Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii","authors":"Xue Liu , Lingxia Zhou , Biyun Luo , Hongli Qian , Baoqing Ye , Keyi Ma , Gaofeng Qiu","doi":"10.1016/j.aaf.2022.01.003","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.01.003","url":null,"abstract":"<div><p>The giant freshwater prawn (<em>Macrobrachium rosenbergii</em>) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of <em>M. rosenbergii</em>, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of <em>M. rosenbergii</em>.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tingwen Cui , Jiaqi Wang , Zhongjun Hu , Xiaowu Chen
{"title":"Expression of gastrin and cholecystokinin B receptor in Lateolabrax maculatus","authors":"Tingwen Cui , Jiaqi Wang , Zhongjun Hu , Xiaowu Chen","doi":"10.1016/j.aaf.2021.11.002","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.11.002","url":null,"abstract":"<div><p>Gastrin (gas) is a peptide hormone that stimulates gastric acid secretion by gastric parietal cells and stimulates gastric motility. The cholecystokinin B receptor (cckbr) can act as a receptor for gastrin, conveying regulatory information on gastrin, but there are fewer studies on its function in fish. The <em>Lateolabrax maculatus</em> is one of the marine aquaculture species in China, it widely distribute in coastal areas. In the study, we cloned the genes of <em>Lateolabrax maculatus</em> gastrin (Lm-gas) and <em>Lateolabrax maculatus</em> cholecystokinin B receptor (Lm-cckbr). The results showed that the full-length gene of Lm-gas is 638bp and the carboxy-terminal conserved domain (DFGRR) is the core functional domain of gastrin protein. The Lm-cckbr gene has a total nucleotide sequence of 2066 bp, and the open reading frame encodes a total of 453 amino acids. The result of protein sequence alignment showed that the similarity between Lm-cckbr protein and other different species was 50.11%–89.67%. The PCR results showed that Lm-gas and Lm-cckbr were expressed in brain and stomach. Further localization by immunehistochemical staining showed that Lm-gas protein was located in the mucosal layer of the gastric wall, but the expression signal was weak in the brain. Hunger causeed a significant decrease in these two genes. The results provided basic research data for further study on the function of Lm-gas and its recepter Lm-cckbr in the in the central nervous system and digestive system of <em>Lateolabrax maculatus</em>.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49766743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Predicting the shelf life of Trachinotus ovatus during frozen storage using a back propagation (BP) neural network model","authors":"Weiqing Lan , Xin Yang , Taoshuo Gong , Jing Xie","doi":"10.1016/j.aaf.2021.12.016","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.12.016","url":null,"abstract":"<div><p>The research aimed to create a shelf life prediction model for <em>Trachinotus ovatus</em> in different freezing temperatures by using back propagation (BP) neural network model. The pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), water retention (water holding capacity [WHC]; cooking loss), and sensory evaluation were measured under 266 K, 255 K, 243 K, 233 K, and 218 K temperatures. The results of TVB-N and water retention during 266 K, 255 K, 233 K, and 218 K conditions were selected to build a BP neural network model and verify the model at 243 K. Results indicated that low temperatures retarded the rise of pH, TVB-N, and TBA values, improving water retention capacity of <em>Trachinotus ovatus</em>. The BP neural network model had high regression coefficients (<em>r</em><sup>2</sup>: 0.8642–0.9904), low mean square error (MES: 0.1658–1.7882), and relative error within 10% and could accurately predict the quality change of <em>Trachinotus ovatus</em> under the freezing temperatures of 266 K–218 K. Therefore, (BP) neural network model has great potential in predicting the shelf life of <em>Trachinotus ovatus</em> in frozen storage.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jilei Zhang , Shuwei Zhang , Kangle Lu , Ling Wang , Kai Song , Xueshan Li , Chunxiao Zhang , Samad Rahimnejad
{"title":"Effects of dietary phosphorus level on growth, body composition, liver histology and lipid metabolism of spotted seabass (Lateolabrax maculatus) reared in freshwater","authors":"Jilei Zhang , Shuwei Zhang , Kangle Lu , Ling Wang , Kai Song , Xueshan Li , Chunxiao Zhang , Samad Rahimnejad","doi":"10.1016/j.aaf.2022.02.004","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.02.004","url":null,"abstract":"<div><p>The present study was conducted to determine the effects of dietary phosphorus (P) levels on growth performance, body composition, liver histology and enzymatic activity, and expression of lipid metabolism-related genes in spotted seabass (<em>Lateolabrax maculatus</em>). Seven diets were prepared to contain available P levels of 0.48% (the control group), 0.69%, 0.89%, 1.10%, 1.28%, 1.51% and 1.77% and feed fish (4.26 ± 0.03 g) to satiety twice daily for 10 weeks. Significantly higher weight gain and specific growth rate were recorded at P levels of 0.69%–1.51% compared to the control group. Feed conversion ratio decreased with increasing P levels up to 0.89% and increased thereafter. The lowest liver lipid content, viscerosomatic index and lipid content of whole-body were obtained in the 0.89%-P group among dietary treatments. P and calcium (Ca) contents in whole body were increased, while liver triglyceride and cholesterol contents were decreased with increasing dietary P levels from 0.48% to 1.77%. The highest activity of hepatic lipase was recorded in the 1.10%-P group among dietary treatments. Compared to the control group, 1.10% P enhanced the proportion of HUFA and reduced the proportion of SFA and MUFA. The histological observations showed that P deficiency (0.48%) led to the vacuolization of hepatocytes and increased number of lipid droplets. Meanwhile, overall liver tissue structure was improved when P level increased to 1.28%. Compared to the control group, expression of lipid metabolism-related genes such as <em>FAS</em>, <em>ACC-2</em> and <em>SREBP-1</em> was decreased at 0.89%–1.10% P group while an opposite trend was observed in the expression of <em>PPARa2</em> and <em>CPT-1</em> genes. The current study showed that 0.89% dietary P levels could promote growth performance of spotted seabass and reduce lipid accumulation in the liver. A broken-line regression analysis based on weight gain showed that the optimum dietary P level (available P) for juvenile spotted seabass reared in freshwater was 0.72%.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seaweed farming for food and nutritional security, climate change mitigation and adaptation, and women empowerment: A review","authors":"Fahmida Sultana , Md Abdul Wahab , Md Nahiduzzaman , Md Mohiuddin , Mohammad Zafar Iqbal , Abrar Shakil , Abdullah-Al Mamun , Md Sadequr Rahman Khan , LiLian Wong , Md Asaduzzaman","doi":"10.1016/j.aaf.2022.09.001","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.09.001","url":null,"abstract":"<div><p>Seaweed is a promising marine macroalgae of the millennium, providing various ecological, social, and economic benefits. At present, seaweed production reached 35.8 million t from farming, accounting for 97% of global seaweed output, with a world market of US$ 11.8 billion. Seaweeds are an excellent source of nutritious human food because of their low lipid content, high minerals, fibers, polyunsaturated fatty acids, polysaccharides, vitamins, and bioactive compounds. Many seaweed sub-products offer unique properties to develop various functional foods for the food processing industries. In the perspective of climate change mitigation, seaweed farms absorb carbon, serve as a CO<sub>2</sub> sink and reduce agricultural emissions by providing raw materials for biofuel production and livestock feed. Seaweed farming system also helps in climate change adaptation by absorbing wave energy, safeguarding shorelines, raising the pH of the surrounding water, and oxygenating the waters to minimize the impacts of ocean acidification and hypoxia on a localized scale. Moreover, it contributes substantially to the sustainable development of the economic condition of coastal women by providing livelihood opportunities and ensuring financial solvency. This review paper highlights the significance of seaweed farming in global food and nutritional security, mitigation and adaptation to global climate change, and women empowerment within a single frame. This review paper also outlined the major issues and challenges of seaweed farming for obtaining maximum benefits in these aspects. The main challenges of making seaweed as a staple diet to millions of people include producing suitable species of seaweeds, making seaweed products accessible, affordable, nutritionally balanced, and attractive to the consumers. Various food products must be developed from seaweeds that may be considered equivalent to the foods consumed by humans today. Lack of effective marine spatial planning to avoid user conflicts is vital for expanding the seaweed farming systems to provide aquatic foods and contribute globally for mitigation and adaptation of climate change impacts. Hence, women's empowerment through seaweed farming is primarily constrained by the lack of technical knowledge and financial resources to establish the coastal farming system. All the information discussed in this paper will help to understand the critical needs for large-scale seaweed farming for climate resilience mariculture, potentials for global food security, and future research on various aspects of seaweed farming and their diverse utilization.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49728156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An evaluation of a catch and release trout regulation in Rapid Creek, South Dakota","authors":"Jacob L. Davis","doi":"10.1016/j.aaf.2020.04.004","DOIUrl":"https://doi.org/10.1016/j.aaf.2020.04.004","url":null,"abstract":"<div><p>Often, catch and release regulations are meant to maximize a fisheries potential to create trophy fish. I evaluated adult Brown Trout populations at five locations within Rapid Creek within Rapid City, SD over a two-year period. In each year, one of these locations was within a catch and release area, while two were outside. In 2012, density and biomass estimates were higher in a site located outside of the catch and release area. Additionally, size structure within the catch and release area was only significantly larger than one of the two sites outside of the regulation area. Mean relative weight values were inversely related to density estimates, with the lowest density sites having the highest mean relative weights. Similarly, a site outside of the catch and release area had the highest abundance and biomass estimates in 2013. The cumulative length distribution was significantly different between the site within the catch and release area and the two located outside. Relative weight was significantly higher in the catch and release area than the two sites outside. As population characteristics within the catch and release area were not consistently better than outside, it is more likely that factors such as habitat and density dependence are impacting the Brown Trout population more than the regulation itself.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aaf.2020.04.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chantelle Clain, John Stewart, Ashley Fowler, Sandra Diamond
{"title":"Age, growth and length-to-weight relationship of largehead hairtail (Trichiurus lepturus) in south-eastern Australia suggest a distinct population","authors":"Chantelle Clain, John Stewart, Ashley Fowler, Sandra Diamond","doi":"10.1016/j.aaf.2023.08.005","DOIUrl":"https://doi.org/10.1016/j.aaf.2023.08.005","url":null,"abstract":"The age, growth rates and length to weight relationships for the south-eastern Australian population of largehead hairtail (Trichiurus lepturus) were investigated for the first time. Age was estimated by counting annuli in sectioned sagittal otoliths, with a maximum age of 8 years estimated for males and females. We found no significant differences in the growth rates of males and females over the size (12–78 cm pre-anal length (PL)) and age (0–8 years) ranges sampled. However, females in our collection were larger on average than males, and all fish sampled >60 cm PL were female, suggesting that females may grow to larger sizes than males and that sampling of greater numbers of larger and older fish may be required in future. T. lepturus growth was described by the von Bertalanffy growth function parameters L∝ = 74.89 cm PL, K = 0.13yr−1 and t0 = −0.80 yr, with the L∝ being amongst the largest reported for the species. Growth was variable, with the sizes at any given age spanning up to 50 cm PL. Female T. lepturus increased in body weight relative to length faster than males, as has been reported in other populations. The length/weight relationship for the south-eastern Australian population was significantly different, with almost no overlap, from that reported for T. lepturus in the Arabian Sea. The substantial differences in growth rates, maximum sizes and body morphometrics of T. lepturus from south-eastern Australia in comparison to other populations globally are consistent with the hypotheses that it represents a distinct population, although investigations using additional population markers are required to verify this.","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongji Huang , Cheng Zhou , Liuxiong Xu , Jiangfeng Zhu , Xuefang Wang , Jie Cao
{"title":"Spatial variation in bigeye tuna Thunnus obesus size at sexual maturity in the eastern Pacific Ocean","authors":"Hongji Huang , Cheng Zhou , Liuxiong Xu , Jiangfeng Zhu , Xuefang Wang , Jie Cao","doi":"10.1016/j.aaf.2021.11.003","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.11.003","url":null,"abstract":"<div><p>Understanding the reproductive characteristics of a species is of crucial for accurate stock assessment and management plans to ensure sustainable fisheries. In this study, the size at 50% sexual maturity (<em>L</em><sub>50</sub>) parameters in different bio-ecological provinces were estimated for bigeye tuna, <em>Thunnus obesus</em>, sampled from the Eastern Pacific Ocean tuna fisheries-dependent survey from 2013 to 2019. The overall sex ratio of the catch during the sampling differed significantly from 1:1. Bigeye tuna exhibit sexual dimorphism in the growth of males and females, with a clear shift in predominance from female to male with increasing sizes. In the North Pacific Sub-tropical Gyre (east) (NPST-east), North Pacific Tropical Gyre (NPTG), Pacific North Equatorial Countercurrent (PNEC), and Pacific Equatorial Divergence (PEQD), females (meals) reached sexual maturity round 102 cm (106 cm), 106 cm (100 cm), 125 cm (110 cm), and 113 cm (110 cm), respectively, the estimated <em>L</em><sub>50</sub> of bigeye tuna was 124.08 cm, 121.97 cm, 139.92 cm and 132.45 cm, respectively. The degree of populations mixing between equatorial (PNEC and PEQD) and high-latitude regions (NPST-east and NPTG) is extremely small, but it is reasonably high between the NPST-east and NPTG or PNEC and PEQD. These parameters were significantly different, suggesting the occurrence of a spatial difference in the size-at-maturity of bigeye tuna between these bio-ecological provinces. The findings of this study provide the key information for understanding the life history of bigeye tuna in the Eastern Pacific Ocean and will contribute to the conservation and sustainable yield of this species.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49842016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The competitiveness of China's seaweed products in the international market from 2002 to 2017","authors":"Huiyu Kang, Zhengyong Yang, Zhiyi Zhang","doi":"10.1016/j.aaf.2021.10.003","DOIUrl":"https://doi.org/10.1016/j.aaf.2021.10.003","url":null,"abstract":"<div><p>China is the most productive seaweed farming country in the world and her seaweed imports and exports have a significant impact on global seaweed trade commodities and food security. Nevertheless, few studies had delved into the main characteristics and development of China's seaweed farming industry. This paper aims to narrow this research gap by analyzing trade patterns in China's seaweed products and the international competitiveness of seaweed product by using the international market share index (IMS), the trade competitiveness index (TC), and revealed comparative advantage index (RCA) from 2002 to 2017. The results showed that Japan, the Association of Southeast Asian Nations (ASEAN), Chile, Peru, and the Republic of Korea are China's main trade partners, the total trade value of China's seaweed products has grown rapidly, its imports have gradually exceeded exports, the trade deficit continues to expand, and its competitiveness keeps decreasing. The reasons for this trend may include the changing seaweed trade commodity structure, product differentiation, increased government support in competing countries, and trade barriers among the trade partners. We propose actively expanding international trade markets, establishing a strong seaweed product processing industry to develop high value-added seaweed products, optimizing the trade commodity structure, and differentiating of seaweed products to improve the competitiveness of China's seaweed products.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melinda Mei Lin Lau , Leonard Whye Kit Lim , Hung Hui Chung , Han Ming Gan
{"title":"The first draft genome assembly and data analysis of the Malaysian mahseer (Tor tambroides)","authors":"Melinda Mei Lin Lau , Leonard Whye Kit Lim , Hung Hui Chung , Han Ming Gan","doi":"10.1016/j.aaf.2022.05.002","DOIUrl":"https://doi.org/10.1016/j.aaf.2022.05.002","url":null,"abstract":"<div><p>The Malaysian mahseer (<em>Tor tambroides</em>), one of the most valuable freshwater fish in the world, is mainly targeted for human consumption. The mitogenomic data of this species is available to date, but the genomic information is still lacking. For the first time, we sequenced the whole genome of an adult fish on both Illumina and Nanopore platforms. The hybrid genome assembly had resulted in a sum of 1.23 Gb genomic sequence from the 44,726 contigs found with 44 kb N50 length and BUSCO genome completeness of 87.6%. Four types of SSRs had been detected and identified within the genome with a greater AT abundance than that of GC. Predicted protein sequences had been functionally annotated to public databases, namely GO, KEGG and COG. A maximum likelihood phylogenomic tree containing 52 Actinopterygii species and one Sarcopterygii species as outgroup was constructed, providing first insights into the genome-based evolutionary relationship of <em>T. tambroides</em> with other ray-finned fish. These data are crucial in facilitating the study of population genomics, species identification, morphological variations, and evolutionary biology, which are helpful in the conservation of this species.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49699860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}