Aquaculture and Fisheries最新文献

{"title":"Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis","authors":"Shirin Akter ,&nbsp;Melissa L. Wos-Oxley ,&nbsp;Sarah R. Catalano ,&nbsp;Md Mahbubul Hassan ,&nbsp;Xiaoxu Li ,&nbsp;Andrew P.A. Oxley ,&nbsp;Jian G. Qin","doi":"10.1016/j.aaf.2024.08.001","DOIUrl":"10.1016/j.aaf.2024.08.001","url":null,"abstract":"<div><div>Feed composition affects the gut bacterial community in aquatic species, but little is known about how diet type impacts gut microbiota in molluscan bivalves. This study investigates the impact of feed composition on the gut bacterial community in two commercially important bivalves: Pacific oyster <em>Magallana gigas</em> and Mediterranean mussel <em>Mytilus galloprovincialis</em>. Oysters and mussels were fed with two types of marine algae, live <em>Isochrysis galbana</em> (microalgae) and the dried seaweed powder <em>Ulva</em> sp. (macroalgae) under a laboratory condition for two months. The V1–V2 region of the 16S rDNA was sequenced through an Illumina MiSeq platform to compare the gut bacterial community of oysters and mussels at the start and the end of the feeding trial. Feed composition directly impacted both alpha and beta diversities of gut microbiota, but the degree of the impact differed between bivalve species. The difference in the gut bacterial assemblages of oysters and mussels in the pre- and post-feeding trials indicated that the diet type and host species shape the composition of gut bacteria. Spirochaeta was absent in mussel guts during the pre- and post-feeding trials but was present in oysters, revealing host specificity in colonizing gut bacteria. The results suggest that the diet type and host species can significantly impact the gut bacterial community structure. This study confirms that diet can modulate gut microbial composition in marine filter-feeding organisms, but the extent of dietary modulation depends on feed type and host species.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 852-859"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size selectivity of flatfish in trawl codends","authors":"Zita Bak-Jensen ,&nbsp;Bent Herrmann ,&nbsp;Juan Santos ,&nbsp;Daniel Stepputtis ,&nbsp;Valentina Melli ,&nbsp;Jordan P. Feekings","doi":"10.1016/j.aaf.2024.10.001","DOIUrl":"10.1016/j.aaf.2024.10.001","url":null,"abstract":"<div><div>Flatfish constitute a substantial proportion of the catch in several demersal trawl fisheries across the globe. Therefore, knowledge on how to discriminate between the individuals that are to be captured or released, by size, is important for the sustainability of exploited stocks. Using European plaice (<em>Pleuronectes platessa</em>), flounder (<em>Platichthys flesus</em>), and dab (<em>Limanda limanda</em>) as case-study species, here we investigate how flatfish are selected in trawl codends by experimentally testing the influence of mesh geometry, and its variability, on size selection. Both diamond-mesh and square-mesh codends were tested, as well as three codends where the mesh shape was fixed to minimize its variation during fishing. The most discriminating size selectivity was found with fixed mesh geometry, revealing that variability in mesh openness negatively affects the selectivity of flatfish. Our results further demonstrate that the risk of retaining undersized flatfish tends to increase with increasing mesh opening angle in diamond-mesh codends. Our results also confirm that when fishing with codends of the same nominal mesh size, the square-mesh codend retains significantly higher proportions of undersized flatfish than the traditional diamond-mesh.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 899-910"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economics of small-scale aquaculture farms in Brazilian Northeastern semi-arid region: Characteristics, feasibility, and profitability models","authors":"Vinícius Fellype Cavalcanti de França ,&nbsp;Luis Otávio Brito da Silva ,&nbsp;Emerson José da Silva Oliveira ,&nbsp;Humber Agrelli de Andrade","doi":"10.1016/j.aaf.2024.07.003","DOIUrl":"10.1016/j.aaf.2024.07.003","url":null,"abstract":"<div><div>Inland aquaculture represents a significant component of the aquaculture sector in Brazil. This activity is present in all Brazilian regions, yet the economic feasibility and the relationship between their productive variables and profitability indices remain poorly accessed in Brazil's semi-arid regions. This study aims to characterise, assess the economic feasibility, and evaluate the relationship between productive variables and profitability indices in the aquaculture sector in Feira Nova (Northeast Brazil). The data was collected through interviews with all producers in Feira Nova city and modelled using the software R. The aquaculture sector in Feira Nova is predominantly small-scale and encompasses a wide range of production strategies for shrimp and fish cultures, with profitable outcomes. The models demonstrated that economic and management variables were more closely associated with profitability indices than farm characteristics such as farm size. Feira Nova's profitability indices reinforces the small-scale aquaculture as a promising activity to alleviate poverty and generate wealth in Brazil semi-arid regions. However, governance improvements are necessary to boost the development of inland aquaculture in the country.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 911-920"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of different percentages of water reuse with bioflocs on the composition of the microbiota in the stomach of Penaeus vannamei shrimp","authors":"Natália Pereira San Martin ,&nbsp;Wilson Wasielesky Jr. ,&nbsp;Francesca Cucchi ,&nbsp;Wellica Gomes dos Reis ,&nbsp;Dariano Krummenauer","doi":"10.1016/j.aaf.2024.09.002","DOIUrl":"10.1016/j.aaf.2024.09.002","url":null,"abstract":"<div><div>The objective of this study was to analyze the stomach composition of <em>Penaeus vannamei</em> shrimp in biofloc cultures with varying percentages of reused bioflocs to identify the optimal culture conditions based on microorganism content. Four treatments were employed: clear water (CW), 25% biofloc inoculum (BF 25%), 50% biofloc inoculum (BF 50%), and 100% biofloc inoculum (BF 100%). Bioflocs were reused from mature bioflocs of previous cultures in biofloc treatments. Shrimp performance was monitored weekly through biometrics, and stomach samples were collected at the experiment's onset and conclusion. The samples underwent fixation in 4% formaldehyde for subsequent counting and identification of predominant microorganism groups using an inverted microscope. Bacterial abundance was assessed by fixing, filtering, and staining samples with acridine orange, followed by quantification with an epifluorescence microscope. Water quality parameters remained satisfactory in all treatments. Shrimp stomachs in biofloc systems contained <em>Oocystis</em> sp., ciliates, flagellates, diatoms, free coccoids, free and attached filamentous, <em>Bacillus</em> spp., and <em>Vibrio</em> spp. CW treatment exhibited the highest <em>Vibrio</em> spp. concentration. Overall, reusing bioflocs in all volume percentages effectively maintained water quality and colonized the animal's microbiota, with a recommended minimum reuse percentage of 25% for satisfactory results.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 867-877"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organization, density, and content of collagen in the body wall of sea cucumbers Acaudina rosettis and Phyllophorus sp.","authors":"Nithasya Nabilla ,&nbsp;Imarotus Shofiyah ,&nbsp;Sugiharto ,&nbsp;Dita Alvitasari ,&nbsp;Sri Sumarsih ,&nbsp;Firas Khaleyla ,&nbsp;Ismiliana Wirawati ,&nbsp;Dwi Winarni","doi":"10.1016/j.aaf.2024.09.003","DOIUrl":"10.1016/j.aaf.2024.09.003","url":null,"abstract":"<div><div>Marine collagen has been known to have lower immunogenicity compared to bovine collagen. The primary component in the body wall of sea cucumbers is collagen. This study aimed to investigate the density, bundle organization, and yield of collagen in the two sea cucumber species, <em>Acaudina rosettis</em> and <em>Phyllophorus</em> sp. collected from the Madura Strait, Indonesia. The body wall parts, including the dorsal anterior, dorsal median, dorsal posterior, ventral anterior, ventral median, and ventral posterior, from five samples of each species, were fixed and decalcified. They were histologically processed using the paraffin method before staining them with Van Gieson to visualize collagen in the cross-sections of body wall tissue. Collagen density was determined using the ImageJ software, while collagen bundle organization was examined negatively by utilizing the image editing application. Collagen density data were analyzed using repeated-measures ANOVA (α = 0.05). Collagen content in the body wall of each species was examined using acid solubilization-based collagen extraction, which did not significantly differ between different parts of the body wall in each species (p = 0.161 for <em>A. rosettis</em>, p = 0.095 for <em>Phyllophorus</em> sp.). However, <em>Phyllophorus</em> sp. exhibited a higher collagen density and collagen bundles, which were larger and more organized compared to <em>A</em>. <em>rosettis</em>. The collagen content was higher in <em>A</em>. <em>rosettis</em> (10.07%) compared to <em>Phyllophorus</em> sp. (8.07%). Further study can be performed to explore the potential of collagen from these sea cucumber species for commercial use.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 816-824"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between dietary rapeseed meal, condensed tannin and growth, nutrive metabolism in largemouth bass (Micropterus salmoides)","authors":"Peng Kang ,&nbsp;Xiaoshuang Xia ,&nbsp;Ying Hang ,&nbsp;Xueming Hua","doi":"10.1016/j.aaf.2024.02.004","DOIUrl":"10.1016/j.aaf.2024.02.004","url":null,"abstract":"<div><div>This study evaluated the effects of rapeseed meal (RM) and condensed tannin (CT) on the growth and nutritional metabolism of largemouth bass (<em>Micropterus salmoides</em>), and speculated whether there was interaction between anti-nutritional factors in RM. Eight diets were formulated for 55-day feeding trial, six of which were practical diet groups, including control group (CG), five RM inclusive groups with graded RM levels of 5% (RM5), 10% (RM10), 15% (RM15), 20% (RM20) and 25% (RM25) partially replacing fishmeal, and the other two were semi-purified diets with 0.062% CT (CT5) ≈ the CT content in the RM5 and 0.31% CT (CT25) ≈ the CT content in the RM25. The results of the experiments on replacing fishmeal with RM demonstrated that with the increase of RM substitution level, the weight gain rate (WGR), survival rate (SR) and specific growth rate (SGR) of largemouth bass showed a downward trend. High levels of RM led to the inhibition of digestive enzyme activity in largemouth bass, as well as the manifestation of abnormal markers related to protein, fat, and carbohydrate metabolism in both plasma and liver. RM reduced muscle protein and fat content, although the effect was not statistically significant at 5% RM level. The results of CT concentration experiments showed that the addition of 0.31% CT significantly reduced WGR, SGR, SR, protein efficiency ratio (PER) and gastrointestinal digestive enzyme activity, and significantly increased the feed coefficient ratio (FCR), plasma ammonia (PA), plasma glucose (PG), and hepatic glycogen (HG) and liver triglyceride (TG). The addition of 0.062% CT significantly reduced PG and HG. Supplementation of CT significantly reduced muscle fat. Among the groups of CT supplementation and RM, CT5 exhibited superior WGR, PER and FCR compared to RM5, along with enhanced intestinal trypsin, amylase activity, liver glutamic pyruvic transaminase (GPT) activity and decreased content of HG and liver TG; CT25 exhibited decreased activities of gastric lipase (LPS), enteric trypsin and pepsin, and increased content of total cholesterol (T-CHO) and glucose in plasma in compariosn to RM25, accompanied with no significant change in growth performance. Therefore, largemouth bass exhibited tolerance to a 5% inclusion of RM in the feed without compromising their growth, digestion and metabolic functions. The incorporation of 5% RM, equivalent to a supplemental 0.062% CT, had regulatory effects on the digestion and metabolism of largemouth bass while improving feed utilization. There was an interaction among anti-nutritional factors in RM, with synergistic effect being evident at low RM levels, whereas antagonism more promient at high levels of RM.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 825-835"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141036572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of tumor necrosis factor receptor 1 and its association with LPS-induced apoptosis in Onchidium reevesii","authors":"Kai Wang ,&nbsp;Heding Shen","doi":"10.1016/j.aaf.2024.03.006","DOIUrl":"10.1016/j.aaf.2024.03.006","url":null,"abstract":"<div><div>The sea slug (<em>Onchidium reevesii</em>) inhabits intertidal environments and face environmental pressures and disease threats. The immune system of mollusks plays a crucial role in their defense against pathogens and maintaining homeostasis. The molecular mechanisms underlying its immune defense and response to pathogens remain largely unexplored. This study aimed to isolate and identify the cDNA sequence of tumor necrosis factor receptor 1 (TNFR1) in the sea slug. The obtained cDNA sequence was named OrTNFR1. Like other members of the TNF receptor superfamily, OrTNFR1 contains a signal peptide, TNF homologous domain, and death-related domain. qPCR results showed that OrTNFR1 was highly expressed in the hepatopancreas, blood, and intestines of sea slug. This indicates that OrTNFR1 plays a role in the immune-related tissues of sea slug and is consistent with the broad expression of TNFR1 in bivalves. To investigate the inducible expression pattern and downstream effects of OrTNFR1 in response to lipopolysaccharide (LPS) stimulation, we studied the functional role of the OrTNFR1 gene by stimulating with LPS in vivo and suppressing OrTNFR1 expression using siRNA technology. Our findings demonstrate that LPS stimulation rapidly up-regulates the expression of OrTNFR1 in the hepatopancreas, blood, and intestine of the sea slug. Moreover, si-TNFR1 effectively inhibits LPS-induced TNFR1 expression and its downstream genes. Additionally, we discovered that inhibiting OrTNFR1 reduces LPS-induced cell apoptosis and the expression of apoptosis-associated protein Caspase.</div><div>TNFR1 is involved in the immune defense of the sea slug, and blocking TNFR1 effectively attenuates LPS-induced cell apoptosis through the TNFR1 pathway. This finding suggests that inhibiting TNFR1 in the sea slug may serve as a potential approach to reduce cell apoptosis. As a shell-less living in harsh intertidal environments, TNFR1 likely participates in its innate immunity. Therefore, studying the TNFR1 pathway contributes to understanding the innate immune signaling pathway in the sea slug, further elucidating host defense against pathogens and apoptotic functions.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 804-815"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141043618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the influencing factors of fishery producers' willingness to participate in aquaculture insurance--based on the perspective of subjective norms and risk perception","authors":"Siyu Xu ,&nbsp;Jianjun Wang ,&nbsp;Ning Cao","doi":"10.1016/j.aaf.2024.04.002","DOIUrl":"10.1016/j.aaf.2024.04.002","url":null,"abstract":"<div><div>The development of aquaculture is an important way to ensure the diversification of China's food supply and food safety. As a market-oriented risk management tool, aquaculture insurance plays a vital role in enhancing the risk resistance of China's aquaculture industry and promoting its high-quality development. Although there has been some discussion on aquaculture insurance in existing studies, there is still a lack of research on the influencing factors of fishery producers' willingness to participate in aquaculture insurance. In order to better understand the intrinsic and extrinsic factors affecting fishery producers' participation in aquaculture insurance, in this paper, based on the Theory of Reasoned Action and from the perspectives of external subjective norms and internal risk perceptions, we thoroughly investigate the effects and mechanism of subjective norms and risk perceptions on the willingness of fishery producers to participate in aquaculture insurance, and we further explore the moderating effect of insurance cognition in this relationship, and construct a corresponding theoretical model. By empirically analyzing the sample data of 238 fishery producers in the Yangtze River Delta region, we find that injunctive norms, descriptive norms and risk perception have positive effects on fishery producers' willingness to participate in aquaculture insurance, with descriptive norms having the most significant impact. In addition, insurance cognition positively moderated the effects of subjective norms and risk perception on fishery producers' willingness to participate in aquaculture insurance, and the moderating effect on risk perception and fishery producers' willingness to participate in aquaculture insurance was the most obvious. Based on the above findings, we put forward a series of targeted recommendations, including strengthening the publicity of aquaculture insurance, providing diversified and targeted aquaculture insurance subsidies, constructing grassroots service stations for aquaculture insurance, and strengthening the training of professional knowledge in aquaculture insurance, etc. The above recommendations are intended to provide a scientific theoretical basis and decision-making reference for the high-quality development of the aquaculture industry.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 921-929"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141031280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation pattern of crustacean zooplankton in a bigheaded carps-dominated reservoir","authors":"Jinyi Zhang ,&nbsp;Jiaoyang Duan ,&nbsp;Ji'ao Zhang ,&nbsp;Jianguo An ,&nbsp;Hongyan Zhang ,&nbsp;Zhongjun Hu ,&nbsp;Zhongbing Chen ,&nbsp;Qigen Liu","doi":"10.1016/j.aaf.2024.03.009","DOIUrl":"10.1016/j.aaf.2024.03.009","url":null,"abstract":"<div><div>The relationship between crustacean zooplankton and (planktivorous) fish has not been well clarified in bigheaded-carps-dominated reservoirs, for most of the past studies to explore that kind of knowledge above are often obtained from the studies in temperate lakes and/or shallow lakes. The limitation of these studies is that crustacean zooplankton lack of either large grazers such as the bigheaded carps or the vertical migrating space for zooplankton to avoid predation by planktivorous fish. Reservoirs are more and more important drinking water source for human consumption, so a better environmental management of reservoirs will rely on a better understanding on the relationship between zooplankton and fish in bigheaded carp dominated reservoirs. To help reach that goal, we investigated the structure and variation of zooplankton and studied the relationship between zooplankton and fish in Tangpu reservoir, Zhejiang province, China. Our results showed that the species of crustacean zooplankton were richer. A total of 59 species of crustacean zooplankton were detected, including 33 species of copepods and 26 species of cladoceran, and dominated by larger species. The density of crustacean zooplankton varied between 15.5–68.5 ind./L with an annual average density of 24.9 ± 14.4 ind./L, it is higher in this planktivorous carps dominated deep reservoir than in many carp-less-dominated reservoirs or in shallow lakes. We also found that zooplankton and fish had a differential vertical distribution, i.e., zooplankton occurred 5 m–10 m but fish occurred 0.5 m–10 m in many months of the year. This could well explain why zooplankton were not reduced and not dominated by small species in bigheaded carps dominated reservoir, so we concluded that in planktivorous carp dominated deep reservoir ecosystems, zooplankton might not compete directly with planktivorous carps for phytoplankton, instead by distributing or migrating to deeper water column, they may survive and thrive on the phytoplankton in deeper column and the detritus resulted from the feces of planktivorous fish, thus might be able to coordinately improve water quality with planktivorous fish.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 887-898"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of lipopolysaccharide from fish-derived Aeromonas hydrophila and their immunostimulatory effect on Qihe crucian carp","authors":"Xinxin Liu ,&nbsp;Yiran Wang ,&nbsp;Junli Wang ,&nbsp;Xiao Yan ,&nbsp;Liping Yang ,&nbsp;Chaobin Qin ,&nbsp;Guoxing Nie","doi":"10.1016/j.aaf.2024.04.003","DOIUrl":"10.1016/j.aaf.2024.04.003","url":null,"abstract":"<div><div>Purified lipopolysaccharide (LPS) was first obtained from fish-derived <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>) by hot phenol-water combined with enzymatic hydrolysis. Subsequently, different concentrations of LPS (0, 0.2, 0.4, 0.8, and 1.2 mg/mL) were injected intraperitoneally into Qihe crucian carp (<em>Carassius auratus</em> var. <em>Qihe</em>) with a dose of 3 μL/g body weight to evaluated their immunostimulatory activity by detecting the expression of immune-related genes in the spleen, head kidney, and hepatopancreas. The results showed that the average extraction rate of LPS was about 1.063% based on the precipitation weight of wet bacteria, of which the polysaccharide content was 9.39%. The silver staining band of LPS after SDS-PAGE electrophoresis showed a ladder distribution typically characterized by smooth-type LPS. Protein and nucleic acid electrophoresis results showed very low amounts of residual protein and nucleic acid, and high-performance liquid chromatography (HPLC) results showed that the purity of the extracted <em>A. hydrophila</em> LPS was similar to that of high-purity commercial LPS. Pro-inflammatory cytokine genes such as <em>IL-1</em>, <em>IL-8</em>, <em>TNF-α</em>, and <em>IL-6</em> were more sensitive to LPS stimulation than anti-inflammatory factors such as <em>IL-10</em> and <em>TGF-β</em>. After 2 h of injection, their expression levels in the spleen, head kidney, and hepatopancreas were significantly increased (<em>P</em> &lt; 0.05), with the 0.8 and 1.2 mg/mL injection groups showing the most significant increase. The gene expression of <em>IL-10</em> and <em>TGF-β</em> showed slight fluctuations in response to <em>A. hydrophila</em> LPS stimulation (<em>P</em> &lt; 0.05). In conclusion, high-purity LPS was extracted from <em>A. hydrophila</em> derived from fish by modified hot phenol-water method, it demonstrating that LPS product could significantly regulate the innate immunity of Qihe crucian carp, with the most effective vaccination dose being 2 mg/kg/BW. These findings provide useful information for future research on endotoxin vaccines.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 5","pages":"Pages 843-851"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141026817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}